1,720,997 research outputs found
3D scaffolds prepared from platelet rich plasma versus conventional plastic surface for culturing adipose-derived canine mesenchymal stem cells and stromal vascular fraction cells
No full text3D scaffolds prepared from Platelet Rich Plasma versus conventional plastic surface for culturing adipose-derived canine Mesenchymal Stromal Cells and Stromal Vascular Fraction cells.
Suelzu C., Conti V., Basini G., Ramoni R. and Grolli S.
Dipartimento di Scienze Mediche Veterinarie, Università di Parma
OBJECTIVE: Mesenchymal Stem Cells (MSCs) are considered a valuable tool for regenerative medicine applications. Stromal vascular fraction (SVF), a heterogeneous population of cells derived from adipose tissue, represents a valid source of MSCs. We evaluated the ability of canine MSCs and adipose-derived SVF cells to be amplified in vitro by exploiting, in addition to the canonical plastic adhesion, a three-dimensional matrix obtained by gelling Platelet Rich Plasma (PRP) or Platelet Poor Plasma (PPP). The aim was to evaluate the possibility of obtaining autologous preparations capable of supporting MSCs and SVF cells growth for the "point of care" application in the veterinary clinic.
MATERIALS AND METHODS: 1. MSCs growth on plastic surface vs 3D fibrin matrix. Total cell number, cell doubling time and cell doubling number of MSCs grown on plastic surface were compared to MSCs cells grown inside a 3D-fibrin matrix prepared by mixing PPP or PPP (50%v/v), DMEM, thrombin (10% v/v) and calcium gluconate (100mg/ml, 10%v/v). 2. SVF growth on plastic surface vs 3D fibrin matrix. SVF cells prepared by collagenase type-I digestion were cultured either on conventional culture dishes or within a 3D fibrin matrix. Total cell number, cell doubling time and cell doubling number were determined for both culture conditions until P3. 3.Phenotypic characterization by RT-PCR of MSCs and SVF cells. Gene expression was compared between MSCs and SVF grown in different culture conditions, i.e. plastic surface versus 3D fibrin matrix. A set of typical MSCs markers and gene involved in their biological properties were evaluated.
RESULTS: Canine MSCs grow within 3D fibrin-based matrices, demonstrating a shorter doubling time and a higher duplication rate when compared to cells grown on the plastic surface: the number of cells obtained is about seven-fold higher in the 3D environment after 144 hours of culture (p<0.01). Moreover, the cells included herein can be frozen and sub-cultured. In our experimental setup, the use of PRP instead of PPP in the preparation of the matrix does not modify cell replication rate. RT-PCR characterisation of cells cultured within the 3D matrix confirms the expression framework of MSCs markers. Furthermore, 3D environment improves SVF cells replication rate, producing at the first culture passage, a mean 1.5-fold increase in cell number.
CONCLUSION: 3D matrices prepared with autologous PRP or PPP are suitable for canine MSCs and SVF cells cultures. Cells grow faster than in standard 2D culture on the plastic surface, while they maintain their panel of gene expression. Furthermore, they can be sub-cultured and frozen for future applications. These results could contribute to set-up more effective MSCs-based therapies, with advantages regarding time shortening for the production of adequate amounts of cells to be applied in the clinical practice
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
Author Under Sail The Imagination of Jack London, 1893-1902
No full textIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Intro -- Title Page -- Copyright Page -- Dedication -- Contents -- Acknowledgments -- Introduction -- 1. Spirit Truth -- 2. From Absorption to Theatricality and Back Again -- 3. "I Will Build a New Present" -- 4. Sons as Authors -- 5. Fathers as Publishers -- 6. The Daughter as Author -- 7. Lovers as Authors -- 8. At Sea with the Family -- 9. Yellow News, Yellow Stories -- 10. The Return Home -- Notes -- Bibliography -- Index -- About Jay WilliamsIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
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