1,514 research outputs found
Rational identification of a colorectal cancer targeting peptide through phage display
No full textColorectal cancer is frequently diagnosed at an advanced stage due to the absence of early clinical indicators. Hence, the identification of new targeting molecules is crucial for an early detection and development of targeted therapies. This study aimed to identify and characterize novel peptides specific for the colorectal cancer cell line RKO using a phage-displayed peptide library. After four rounds of selection plus a negative step with normal colorectal cells, CCD-841-CoN, there was an obvious phage enrichment that specifically bound to RKO cells. Cell-based enzyme-linked immunosorbent assay (ELISA) was performed to assess the most specific peptides leading to the selection of the peptide sequence CPKSNNGVC. Through fluorescence microscopy and cytometry, the synthetic peptide RKOpep was shown to specifically bind to RKO cells, as well as to other human colorectal cancer cells including Caco-2, HCT 116 and HCT-15, but not to the normal non-cancer cells. Moreover, it was shown that RKOpep specifically targeted human colorectal cancer cell tissues. A bioinformatics analysis suggested that the RKOpep targets the monocarboxylate transporter 1, which has been implicated in colorectal cancer progression and prognosis, proven through gene knockdown approaches and shown by immunocytochemistry co-localization studies. The peptide herein identified can be a potential candidate for targeted therapies for colorectal cancer.Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2019 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte and the Project FCOMP-01–0124-FEDER-021053 (PTDC/SAU-BMA/121028/2010). Débora Ferreira is recipient of a fellowship supported by a doctoral advanced training (call NORTE-69-2015-15) funded by the European Social Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. Franklin L. Nobrega, Sara Granja and Ligia R Rodrigues acknowledge FCT for the grants SFRH/BD/86462/2012, SFRH/BPD/117858/2016 and SFRH/BSAB/142991/2018, respectively. Catarina Barbosa-Matos also acknowledge her research grant UMINHO/BI/395/2018info:eu-repo/semantics/publishedVersio
Análise de canais, fluxos e protagonismos: um estudo de caso sobre a comunicação interna na Embrapa caprinos e ovinos.
No full textResumo: O artigo traça uma avaliação de projeto de Comunicação Interna na Embrapa Caprinos e Ovinos, observando o impacto das mudanças em canais, veículos e processos de comunicação, por meio da pesquisa junto ao público interno da entidade. Como destaque, os resultados de satisfação e sugestões dos colaboradores, analisados sob a ótica de correntes teóricas da Comunicação Organizacional, para verificar o cumprimento de premissas de uma comunicação interna participativa e de diálogo, prevista como objetivo do projeto
Melhorias de processos, fluxos e canais para comunicação interna: uma análise de caso da Embrapa Caprinos e Ovinos.
No full textResumo: O artigo traz resultados premilinares da implantação do projeto ?Comunicação Interna na Embrapa Caprinos e Ovinos - melhorias de processo, fluxos e canais?, implementado naquela unidade da empresa, situada em Sobral (CE). Na abordagem, estão presentes a caracterização de problemas de comunicação interna que originaram o projeto e a fundamentação teórica de suas ações, em alinhamento com ideias de orientação estratégica para comunicação interna e com valores e diretrizes para uma comunicação interna que se pretende eficiente, democrática e plural
Complete genome sequences of two T4-like Escherichia coli bacteriophages
No full textBacteriophages and their proteins have potential applications in biotechnology for the detection and control of bacterial diseases. Here, we describe the sequencing and genome annotations of two strictly virulent Escherichia coli bacteriophages that may be explored for biocontrol strategies and to expand the understanding of phage-host interactions.BN/Stan Brouns La
Effects of Bleaching Agents Combined with Regular and Whitening Toothpastes on Surface Roughness and Mineral Content of Enamel
No full textMade available in DSpace on 2019-09-12T16:53:49Z (GMT). No. of bitstreams: 0
Previous issue date: 2015Objective: The purpose of this study was to evaluate surface roughness and changes in the composition of enamel submitted to different bleaching protocols and toothbrushing with regular and whitening toothpastes. Background data: Bleaching treatment could promote morphological and chemical changes in enamel surface. Methods: Enamel blocks were randomized into nine groups (n=10) according to the bleaching treatment (no bleaching, control group; 6% hydrogen peroxide, HP; or 10% carbamide peroxide, CP) and toothpaste used (placebo, PL; regular, R; or whitening dentifrice, W). Bleaching was performed according to manufacturers' instructions and all groups were submitted to 30,000 cycles of simulated toothbrushing with toothpaste (PL, R, or W). Mineral content evaluation and enamel roughness were evaluated initially (T-1), after bleaching (T-2), and after toothbrushing (T-3), using an energy-dispersive micro X-ray fluorescence spectrometer and profilometry, respectively. Data were statistically analyzed with two way ANOVA, Tukey, and Dunnett tests (5%). Results: Enamel surface roughness was influenced by bleaching and toothbrushing. Surface roughness increased for the groups that brushed with the placebo dentifrice (CP+PL, HP+PL, C+PL) and for the control group that brushed with whitening dentifrice (C+W). Enamel Ca/P ratio decreased after bleaching, but toothbrushing, regardless of the dentifrice used, did not reduce the enamel mineral content. Conclusions: The bleaching treatment resulted in a decrease of enamel mineral content, but the studied dentifrices did not contribute to surface mineral loss.[Attia, Mariana Lerner; Cavalli, Vanessa; Freitas D'Arce, Maria Beatriz; do Rego, Marcos Augusto; Suzy Liporoni, Priscila Christiane] Universidade de Taubaté (Unitau), Dept Restorat Dent, BR-12020330 Taubate, SP, Brazil[do Espirito Santo, Ana Maria] Univ Fed Sao Paulo, Dept Math & Earth Sci, Diadema, SP, Brazil[Martin, Airton Abrahao] Univ Paraiba Valley, Res & Dev Inst IP&D, Sao Jose Dos Campos, SP, Brazil[Baggio Aguiar, Flavio Henrique; Lovadino, Jose Roberto; Suzy Liporoni, Priscila Christiane] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Restorat Dent, Piracicaba, SP, Brazil[Cavalcanti, Andrea Nobrega] Univ Fed Bahia, Sch Med & Publ Hlth Bahia, Sch Dent, Area Restorat Dent, Salvador, BA, Brazil[Cavalcanti, Andrea Nobrega] Univ Fed Bahia, Sch Dent, Salvador, BA, Brazi
Status of the Test System of the MWPC for the LHCb Muon System
No full textThis document describes the apparatus and the procedure used to test the 1368 Multi Wire Proportional Chambers (MWPC) after front-end assembly for the LHCb Muon System. Given a fully equipped chamber, this system is able to diagnose every channel through an analysis of front-end output drivers and noise rate versus threshold response. In addition, it verifies if the noise rate at the operational threshold is within appropriate limits. Aiming at an automatic, fast and userfriendly system for mass production tests of MWPC, the project has foreseen as well electronic identification of every chamber and front-end board, and data archiving in such a way to make it available to the Experiment Control System (ECS). The results obtained on about 130 chambers tested are described
Physicochemical changes in enamel submitted to pH cycling and bleaching treatment
No full textMade available in DSpace on 2019-09-12T16:57:00Z (GMT). No. of bitstreams: 0
Previous issue date: 2018Objective: The purpose of this study was to assess the hardness, mineral content, surface roughness, and micromorphology of sound and slightly demineralized enamels, before and after bleaching procedure using 10% carbamide peroxide. Methods: Sixty bovine dental blocks were randomly divided into the following two groups: 30 slabs were submitted to three cycles of pH and 30 slabs were noncycled. Hardness (n=10) was measured using the microhardness tester with Knoop indenter under a 50 g load for 5 seconds. The calcium/phosphate (Ca/P) ratio (n=10) was obtained using a micro-energy-dispersive X-ray fluorescence (mu-EDXRF) spectrometer. The measurement of roughness average (n=10) was performed using a surface roughness tester. Specimens were bleached 6 hours/day during 21 days, and then, physicochemical properties were re-evaluated. Two additional specimens were carried out to evaluate surface micromorphology using the scanning electron microscopy. Data were statistically analyzed by ANOVA and Tukey's test (alpha=0.05). Results: Sound and slightly demineralized enamels showed no difference in Ca/P ratio after dental bleaching according to the mu-EDXRF analysis, but the Ca/P ratio decreased after bleaching for slightly demineralized enamel. Bleaching treatment decreased the hardness and increased the surface roughness, causing micromorphology alterations. Conclusion: Therefore, bleaching procedure promoted change in bovine enamel surface and increased the demineralization of slightly demineralized enamel but not affected the mineral content of sound enamel.[Eskelsen, Evania; Catelan, Anderson; Suzy Liporoni, Priscila Christiane] Universidade de Taubaté (Unitau), Dept Dent, R Expedicionario Ernesto Pereira 110, BR-12020330 Taubate, SP, Brazil[Aparecida Pinto Hernades, Natalia Maria; Baggio Aguiar, Flavio Henrique] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Restorat Dent, Piracicaba, SP, Brazil[Silva Soares, Luis Eduardo] Univ Vale Paraiba, Res & Dev Inst, Lab Biomed Vibrat Spect, Sao Jose Dos Campos, SP, Brazil[Cavalcanti, Andrea Nobrega] Sch Med & Publ Hlth Bahia, Dept Restorat Dent, Salvador, BA, Brazil[Cavalcanti, Andrea Nobrega] Univ Fed Bahia, Salvador, BA, Brazi
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