10,538 research outputs found
Characterisation of Bombyx mori odorant-binding proteins reveals that a general odorant-binding protein discriminates between sex pheromone components
No full textIn many insect species, odorant-binding proteins (OBPs) are thought to be responsible for the transport of pheromones and other semiochemicals across the sensillum lymph to the olfactory receptors (ORs) within the antennal sensilla. In the silkworm Bombyx mori, the OBPs are subdivided into three main subfamilies; pheromone-binding proteins (PBPs), general odorant-binding proteins (GOBPs) and antennal-binding proteins (ABPs). We used the MotifSearch algorithm to search for genes encoding putative OBPs in B. mori and found 13, many fewer than are found in the genomes of fruit flies and mosquitoes. The 13 genes include seven new ABP-like OBPs as well as the previously identified PBPs (three), GOBPs (two) and ABPx. Quantitative examination of transcript levels showed that BmorPBP1, BmorGOBP1, BmorGOBP2 and BmorABPx are expressed at very high levels in the antennae and so could be involved in olfaction. A new two-phase binding assay, along with other established assays, showed that BmorPBP1, BmorPBP2, BmorGOBP2 and BmorABPx all bind to the B. mori sex pheromone component (10E,12Z)-hexadecadien-1-ol (bombykol). BmorPBP1, BmorPBP2 and BmorABPx also bind the pheromone component (10E,12Z)-hexadecadienal (bombykal) equally well, whereas BmorGOBP2 can discriminate between bombykol and bombykal. X-ray structures show that when bombykol is bound to BmorGOBP2 it adopts a different conformation from that found when it binds to BmorPBP1. Binding to BmorGOBP2 involves hydrogen bonding to Arg110 rather than to Ser56 as found for BmorPBP1
Memento Mori
No full textMemento Mori explora terreno muerto bajo las reglas de los vivos, una búsqueda constante de sacralizar lo profano, re significar el rechazo concebido y el deseo de mantener oculta a la muerte misma.Pregrad
Um modelo matematico para otimização da produção de casulos
No full textDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro TecnologicoO presente trabalho fornece uma contribuição à atividade sericícola, abordando aspectos como fertilização do solo e técnicas de criação do bicho-da-seda. A meta é utilizar tais técnicas de forma a obter o máximo lucro possível na produção. A metodologia baseia-se na construção de um modelo matemático para programação não linear, cujo objetivo é fornecer medidas adequadas dos elementos empregados na criação, tais como número de tratos diários, número máximo de larvas por metro quadrado, etc, para atingir a meta acima citada
Use of fuzzy algorithms for phonetic and phonemic labeling of continuous speech
No full textA model for assigning phonetic and phonemic labels to speech segments is presented. The system executes fuzzy algorithms that assign degrees of worthiness to structured interpretations of syllabic segments extracted from the signal of a spoken sentence. The knowledge source is a series of syntactic rules whose syntactic categories are phonetic and phonemic features detected by a precategorical and a categorical classification of speech sounds. Rules inferred from experiments and results for male and female voices are presented
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