47 research outputs found
Translational process engineering for tissue engineered hollow organ advanced therapy investigational medicinal products
No full textTissue engineering has experienced increasing exposure and translational success in recent times, with tissue engineered products accounting for more than a quarter of approved advanced therapy medicinal products within Europe. Hollow organs represent a key target for developing novel therapies, typified by the recent success reported for tissue engineered hemilaryngeal replacements in a preclinical study. This thesis investigates the translational process engineering required to progress from a preclinical, good laboratory practice (GLP) process, to one that is compliant with good manufacturing practice (GMP) guidelines and suitable for clinical manufacture. A GLP decellularisation protocol was translated to conform to GMP-guidelines by modifying the existing standard operating procedure, and adapting an off-the-shelf bioreactor to form a closed-system for aseptic processing. The process was successfully validated for aseptic operation and decellularisation efficacy evaluated, relative to the preclinical process. The decellularised, human hemilarynx scaffolds produced were demonstrated to support bone marrow mesenchymal stromal cells (BM-MSCs) up to product release. A bespoke, modular bioreactor was designed and fabricated to enable manufacture of hemilarynges at scale. The bioreactor was successfully validated for aseptic use, whilst biocompatibility testing indicated no preclusion to use with BM-MSCs or epithelial cells. Proof-of-principle data supported the concept of epithelial sheet production inside the bioreactor, utilising a sheet-specific cassette. The bioreactor was retrospectively adapted to enable closed-system decellularisation processing of a third tissue-type, juvenile oesophagus. Acellular scaffold biomolecular composition and biomechanics were characterised, preceding implantation in a large-animal model. A second, bespoke bioreactor was designed, manufactured and employed to improve the manufacturing process. The combined human larynx data supported the award of a clinical trial authorisation, whilst the oesophageal work is now transitioning to a pivotal animal study. These findings support the application of bespoke bioreactor systems in process closure and translation towards robust, regulatory compliant, manufacturing processes for tissue engineered products
The sacred choral music of Francis Poulenc: a contextual and analytical study
No full textPoulenc is perhaps best known for his instrumental works, for his adherence to the aesthetics of Neo-classicism, and his place among the Parisian intellectual circles in tJie 1920s and 1930s in which his friend, Jean Cocteau, played a central role. This essentially secular side of Poulenc's creativity was, after the composer's return to Roman Catholicism in 1936, challenged by a need to express a newly-found religious conviction in sacred music. Consequently Poulenc, who had been accustomed to the secular aesthetics of Neo-classicism of Parisian artistic life and the French capital's concert halls, found it necessary to 'rediscover' and assimilate the language of French church music and its history (notably through the filter of the Cecilian Movement, Niedermeyer and the pkinchant of Solesmes) in order to create for himself an appropriate 'sacred style’ that could also incorporate those essential elements of his characteristically playful and sensual, 'secular' language. This study aims to explore this confrontation of styles and how Poulenc successfully forged a cohesive and congruent language for his sacred works. The opening chapters have several distinct perspectives: chapter one outlines the tortuous history of the Church's relationship with the State in France dating back to the pivotal effects of the 1789 Revolution, in an attempt to provide a necessary context for the importance that Poulenc and his predecessors and contemporaries (most significantly Debussy) attached to the past; chapter two, by contrast, discusses some of the principal issues at the heart of Parisian artistic society in the early decades of the twentieth century and focuses on the lively artistic community which existed in Paris with the influx of large numbers of foreign musicians (particularly Americans and Russians) and artists, the emergence of 'Les Six' (of which Poulenc was a member) and the artistic leadership and inspiration given by figures such as Jean Cocteau, Serge Diaghilev and Igor Stravinsky. Cocteau and Stravinsky, indeed, had a huge impact on the young Poulenc. The second part of the thesis is an analytical study of Poulenc's sacred works (putting aside the Gloria, Stabat Mater and Sept Repais de Tetibres which are unmistakably concert works) and connects these analyses with the issues presented in the earlier chapters, beginning with the emotionally powerful Litanies a la vierge noire for women’s voices, composed soon after his Catholic faith returned in 1936, and ending with the decidedly hard-edged, Stravinskian Neo-classicism, yet relative placidity, of the Laudes de Saint Antoine de Padoue for men's voices, completed in Cannes in 1959. Central to the analytical discussion are the well known eclectic Mass in G (1937), the dramatic Quatre motets pour un temps de penitence (1939) and the stylistically distilled Quatre petite prieres de Saint Francois d'Assise which display the greatest variety of style and form and which combine to present significant examples of Poulenc's skilful unification of sacred and secular, ancient and modem sound worlds
Translating men : humanism and masculinity in Renaissance renditions of patristic texts
No full textPhDThis doctoral thesis focusses upon the translation of patristic works into English in
the sixteenth and seventeenth centuries. Considering the pragmatic usage of texts in humanist
culture, this research project explores the mobilisation of vernacular versions of the Church
Fathers in response to historical crises. Regarding Renaissance humanism as a gendered
intellectual methodology,I have investigated the way in which these texts particularly aim to
address the needs of men, offering them exemplars to 'cope' with their social circumstances.
The first chapter involves the analysis of Thomas Drant's rendition of Gregory of
Nazianzus' Epigrams (1568) as part of the struggles of the early Elizabethan era. I suggest
that this verse translation may possibly have played a supportive role for Protestant clerics
facing a loss of humanist confidence due to educational deficiencies and the conflict of
learning with the Catholic Louvainist scholars.
The second chapter examines John Healey's version of Augustine's City of God
(1610) in the context of the colonisation of Virginia. I propose that the Augustinian text - and
the included commentary by Vives - may have represented a 'handbook' for the
predominantly male community of planters confronted by (among other problems) the severe
difficulty of establishing a household and fathering the next generation.
The third chapter looks at Tobie Matthew's translation of Augustine's Confessions
(1620) as an aid for Catholic Englishmen in an age of religious persecution. I contend that
this text advertises and advances a passive / feminine form of manhood - which had been
initially propagated by late sixteenth-century recusant ideology - in order to offer succour to
its socially debilitated male readers.
By undertaking an examination of these previously neglected texts, this thesis has
attempted to expand the understanding of Renaissance humanist translation, as well as to
offer a unique insight into the history of gender
All About Eve
No full textFasten your seat belts folks!
With 90 years of the Academy Awards to choose from, Paul Gambaccini is back with a swashbuckling, all singing, all dancing, behind the scenes new series of AND THE ACADEMY AWARD GOES TO... digging up the stories behind the greatest Best Picture Oscar winners - reflecting the world in which they were made.
With "La La Land" a hot favourite at this year's awards, with 14 nominations, Gambaccini takes as his first subject a similarly feted film from 1950.
"All About Eve" was Bette Davis's come back picture, as she took on the role of Margo Channing, a Broadway star on the wrong side of 40. Davis had been Hollywood divinity, but the previous year she had won Worst Actress of the Year from the San Francisco Critics Circle.
Even back then navel gazing was a favourite Hollywood obsession, and the Academy had to weigh up the merits of two films about show business - "All About Eve" and "Sunset Boulevard".
With 14 nominations and 6 wins, "All About Eve" carried the day - but the night was a keen disappointment for Davis, who was hoping for a third Oscar, as her co-star, Anne Baxter, would cancel out her chances of winning Best Actress.
Michael Merrill, Davis's son, recalls her bitter disappointment, but it's still his favourite film - "Because it was how my mother and father met each other".
Gary Merrill, Davis's on screen love interest, would become her 3rd husband.
Paul also hears from Cheryl Bray Lower - biographer of director Joseph L Mankiewicz, Ed Sikov - author of "Dark Victory - The Life Of Bette Davies", film writer David Thomson, critic Larushka Ivan Zadeh, and Professor Toby Miller.
Is it all about Eve, or all about Bette Davies
Lucian Blaga: Romanian Spatial Horizons
No full textThe article seeks to present the concept of space of the Romanian thinker Lucian Blaga (1895–1961). The author of the article presents the main assumptions of Blaga’s philosophy of culture, as presented in his work Trilogia culturii. One of its premises is the existence of deep, unconscious spatial horizons that condition many basic cultural forms, and which the Romanian philosopher contrasts with surface landscapes, which emerge through direct sensory perception. Blaga’s concept of spatial horizon relies on the analyses of pastoral space (spațiul mioritic): an undulating spatial horizon, a source for the Romanian spirit. The article contrasts Blaga’s thinking with an alternative concept developed by Vasile Băncili, who onsiders the steppe landscape, characteristic of the south-eastern Danubian areas of Romania (Câmpia Bărăganului, Brăgan plain), to be equally significant for Romanian [email protected] Zawadzki, pracuje w Katedrze Komparatystyki Literackiej Wydziału Polonistyki UJ, autor książek Nowoczesna eseistyka filozoficzna w piśmiennictwie polskim I połowy XX wieku (2001), Literatura a myśl słaba (2009), Obraz i ślad (2015). Współredaktor tomów: Myśl mocna myśl słaba. Hermeneutyka włoska w od połowy XX wieku (wspólnie z Monika Surmą-Gawłowską, 2015) oraz Constantin Noica i filozofia XX wieku (wspólnie z Joanną Kornaś-Warwas, 2018).Katedra Komparatystyki Literackiej, Wydziału Polonistyki, Uniwersytetu JagiellońskiegoBăncilă Vasile (2007), Spațiul Bărăganului, w: V. Băncilă, Opere, vol. 7, Brăila: Istros.Blaga Lucian (1985), Trilogia culturii, w: Opere, vol. 9, București: Minerva.Blaga Lucian (1998), Przestrzeń pasterska, przeł. I. Kania, „Literatura na Świecie”, nr 9.Cassirer Ernst (2011), Logika nauk o kulturze, przeł. P. Paszutowicz, Kęty: Wydawnictwo Marek Derewiecki.Głowiński Michał, Kostkiewiczowa Teresa, Okopień-Sławińska Aleksandra, Sławiński Janusz (1998), Słownik terminów literackich, red. J. Sławiński, Wrocław–Warszawa–Kraków: Ossolineum.Hartmann Eduard, von (1982), Filozofia nieświadomego. Wybór tekstów, przeł. B. Markiewicz, Warszawa: Wydawnictwo Instytutu Filozofii i Socjologii PAN.Heidegger Martin (2004), Bycie i czas, przeł. B. Baran, Warszawa: Wydawnictwo Naukowe PWN.Kant Immanuel (1986), Krytyka władzy sądzenia, przeł. J. Gałecki, Warszawa: Państwowe Wydawnictwo Naukowe.Leo Frobenius în România | Veni, vidi, velo!, archive.org/web/20130601225527/http:/ciclist.wordpress.com/2012/06/25/leo-frobenius-in-romania/ [dostęp 31.08.2021].Liiceanu Gabriel (2012), 24 cuvânte cheie ale lui Heidegger, București: Humanitas.Noica Constantin (1996), Cuvânt împreună despre rostirea românească, București: Humanitas.Pakalski Dariusz (2013), Estetyka Eduarda von Hartmanna, Toruń: Wydawnictwo Tako.Pareyson Luigi (2014), Estetica dell’idealismo tedesco, t. 2: Fichte e Novalis, Milano: Mursia.Schelling Friedrich Wilhelm Joseph (1979), System idealizmu transcendentalnego, przeł. K. Krzemień, Warszawa: Państwowe Wydawnictwo Naukowe.Schelling Friedrich Wilhelm Joseph (2015), Filozofia sztuki, przeł. K. Krzemień, Warszawa: Państwowe Wydawnictwo Naukowe.Spengler Oswald (2014), Zmierzch Zachodu: zarys morfologii historii powszechnej, przeł. J. Marzęcki, Warszawa: Wydawnictwo Aletheia.Wilkinson Toby (2019), Powstanie i upadek starożytnego Egiptu: dzieje cywilizacji od 3000 p.n.e do czasów Kleopatry, przeł. N. Radomski, Poznań: Dom Wydawniczy Rebis.Zimmer Heinrich (2014), Mity i symbole w indyjskiej sztuce i kulturze, przeł. M. Szymański, Warszawa: Wydawnictwo Aletheia.2511512
Can the Theory of Planned Behaviour Improve Our Understanding of the Influence of Organisational Factors on Workers' Behaviour?
No full textCopyright 2007 the Australian Psychological Society. Author version reproduced here with permission from the publisher. This is an electronic version of an article published in 'Freeman, T., Roche, A.M., Williamson, P., & Pidd, K. (2007). Can the Theory of Planned Behaviour improve our understanding of the influence of organisational factors on workers’ behaviour? Proceedings of the 7th Industrial & Organisational Psychology Conference, 81-85.'Understanding and changing workers’ behaviour are key goals of Organisational Psychology. The Theory of Planned Behaviour has the potential to make an important contribution to our understanding of how organisational factors influence workers’ behaviour and of ways to achieve behaviour change with workers. According to the Theory of Planned Behaviour, intentions, attitudes, subjective norms, and perceived behavioural control are the most proximal predictors of behaviour. Any distal variables, such as organisational factors, only influence behaviour through the theoretical predictors. Though a substantial body of research has applied the Theory of Planned Behaviour to the organisational setting, no research to-date has examined whether the Theory of Planned Behaviour accounts for the influence of organisational variables on workers’ behaviour. This paper presents the results of a survey of 273 dental hygienists which applied the Theory of Planned Behaviour to the behaviour of assisting their patients to quit smoking. The findings indicated that organisational factors like the presence of a policy and education or training influenced behaviour only through subjective norms and perceived behavioural control. These results inform understanding of the pathways through which organisational factors influence workers’ behaviour. Practical implications of applying the theory to a wide range of work behaviours are highlighted.Melbourn
De la hache à la tronçonneuse : transformations, résistances et évolution du rapport au territoire chez les Cris de la Baie James (Québec)
No full textThrough an ethnographic approach, characterized by a long and inductive fieldwork, the author of this thesis analyses the transformations faced by the Cree of James Bay (Northern Quebec) following the agreement called the "Peace of the Braves ". The latter, signed in 2002, approved the Eastmain-Rupert hydroelectric project and redefined the partnership between the Cree, the State, Hydro-Québec and various other economic actors. The research displays the multiple aspects of this redefinition, characterized by a renewed relationship between the Crees and their territory, which can be seen as the matrix of their identity. As much their involvement in the megaprojects of resource exploitation as the persistence of their animist ontology and their hunting practices are analyzed. Based on a meticulous and nuanced portrait of the inhabitants of Nemaska, the study also questions the global issues (ontological, social and economic) of territorial agreements and the consequences for Aboriginal people of their increasing integration in the neoliberal model of a globalized Canada.À travers une approche ethnographique, caractérisée par un terrain long et inductif, l'auteure de cette thèse étudie les processus de transformation en cours chez les Cris de la Baie James (Nord du Québec) induits par l'entente dite de la « Paix des Braves ». Cette dernière, signée en 2002, a entériné le projet hydroélectrique Eastmain-Rupert et a redéfini les relations de partenariat entre les Cris, l'État, Hydro-Québec et divers acteurs économiques. La recherche expose les multiples facettes qui, suite à cette redéfinition, caractérisent aujourd'hui le rapport des Cris à leur territoire, matrice de leur identité. Tant leur implication dans les mégaprojets d'exploitation des ressources que la persistance de leur ontologie animiste et de leurs pratiques de chasse y sont analysées. Sur la base d'un portrait fouillé et nuancé des habitants de Nemaska, cette étude questionne également les enjeux globaux (fonciers, ontologiques, sociaux et économiques) des ententes territoriales ainsi que les conséquences pour les Autochtones d'une insertion toujours plus grande dans le modèle néolibéral d'un Canada globalisé.(POLS - Sciences politiques et sociales) -- UCL, 201
A necessary fiction: The ritualisation of stakeholder practices in New Zealand cinema
No full textThis thesis argues that stability of the concept ‘national cinema’ is located in the discursive positioning of individual films in such a way that they are connected to a national ‘common ground’, one which is ritually accessed via engagement with media such as cinema. This positioning, however, is not quantifiable and may not be identified as arising from any particular production practice, dimension of popularity, theme, style, characteristic of production personnel, and so on. By synthesising the work of several theorists and applying this synthesis to a selection of films, a framework of ideas (around the ritualised ‘flagging’ of the national via the expression of stakeholder interests) is applied to cinema in New Zealand. In particular, an ideoscape is ultimately mapped as a result of applying this framework of ideas. The normative assumptions of national cinema are examined in this way and found to be lacking despite the weight that the term ‘national cinema’ continues to have
MCUR1 Is a Scaffold Factor for the MCU Complex Function and Promotes Mitochondrial Bioenergetics
No full textSummaryMitochondrial Ca2+ Uniporter (MCU)-dependent mitochondrial Ca2+ uptake is the primary mechanism for increasing matrix Ca2+ in most cell types. However, a limited understanding of the MCU complex assembly impedes the comprehension of the precise mechanisms underlying MCU activity. Here, we report that mouse cardiomyocytes and endothelial cells lacking MCU regulator 1 (MCUR1) have severely impaired [Ca2+]m uptake and IMCU current. MCUR1 binds to MCU and EMRE and function as a scaffold factor. Our protein binding analyses identified the minimal, highly conserved regions of coiled-coil domain of both MCU and MCUR1 that are necessary for heterooligomeric complex formation. Loss of MCUR1 perturbed MCU heterooligomeric complex and functions as a scaffold factor for the assembly of MCU complex. Vascular endothelial deletion of MCU and MCUR1 impaired mitochondrial bioenergetics, cell proliferation, and migration but elicited autophagy. These studies establish the existence of a MCU complex that assembles at the mitochondrial integral membrane and regulates Ca2+-dependent mitochondrial metabolism
Adaptations of Hamlet in Different Cultural Contexts: Globalisation, Postmodernism, and Altermodernism
No full textAlthough there has traditionally been a resistance to the study of adaptations, adaptation studies as a subsection of 'intertextuality‘ currently has a significant place in academic debates. Hamlet is "the Mona Lisa of literature" (T.S. Eliot), and has been the subject of constant scrutiny, mythologizing and adaptation. Hamlet has been adapted and appropriated into and by various cultural contexts. Even confining our attention to the same medium as Shakespeare‘s text, there exists an array of theatrical adaptations in languages and cultures as diverse as Persian, Korean, Arabic, German, Russian, and Turkish. Borrowing Ludwig Wittgenstein‘s metaphor of 'family resemblance,‘ I argue the usefulness of his idea, enabling us to examine not simply a small number of common properties among adaptations of Hamlet, but rather to explore the 'complicated network of similarities overlapping and criss-crossing‘ (Philosophical Investigations, §66). I further propose subdividing the 'global family‘ of Hamlets from around the world that participate in this/these web-like resemblances into 'local families‘ of adapted Hamlets, to enable better intercultural and cross-cultural studies.
In this thesis I analyse seven theatrical adaptations of Hamlet in Turkish, Russian, Arabic and Persian cultural contexts, from the perspectives of postmodernism, globalisation and altermodernism. I also scrutinise the Persian family of Hamlet in the light of 'intertextuality‘. Given that each adaptation per se brings together 'self‘ and 'other‘ at the same time, I go on to coin two new terms: homointertextuality and heterointertextuality, in order to explore fully the various connections of the adaptations of Hamlet in Iran with the 'cultural self‘ (Persian culture) and the 'cultural other‘ (Anglophone culture)
