830 research outputs found
Mind “De GaPP”: in vitro efficacy of deferiprone and gallium-protoporphyrin against Staphylococcus aureus biofilms
No full textData source: Supporting information, http://onlinelibrary.wiley.com/doi/10.1002/alr.21735/abstract#footer-support-infoAbstract not availableKatharina Richter, Mahnaz Ramezanpour, Nicky Thomas, Clive A. Prestidge, Peter-John Wormald and Sarah Vreugd
Silica-lipid hybrid (SLH) microcapsules: a novel oral delivery system for poorly soluble drugs
No full textAbstract not availableAngel Tan, Spomenka Simovic, Andrew K. Davey, Thomas Rades, Clive A. Prestidg
Clive Small on the real-life "Underbelly"
No full textOver the course of his career, Clive Small, one of NSW\u27s most successful detectives, saw it all. His book, "Smack Express: How organised crime got hooked on drugs" is an insight into drug trafficking and organised crime on Australia\u27s east coast. Written with journalist Tom Gilling, it features an extraordinary range of colourful characters and situations. Take "Aunty", the female drug lord who has been successfully importing kilos of cocaine into Australia for decades. Or the bloke who thought that throwing someone into the boot of a car and driving it to South Australia wasn\u27t kidnapping, because "he never asked to get out of the boot".
Clive Small is a former Assistant Commissioner of Police in NSW, and a former ICAC chief investigator. He resigned from ICAC in 2007 to pursue a defamation case against broadcaster Alan Jones. His investigations included the death of Griffith anti-drugs campaigner Donald McKay, the assassination of Cabramatta MP John Newman and the backpacker murders of Ivan Milat.
Tom Gilling is a former journalist and author. He has written a number of novels and co-authored "The Bagman: Final Confessions of Jack Herbert", about a corrupt Queensland policeman whose evidence in the 1980s Fitzgerald Inquiry had a huge impact on Queensland
Photograph - Burrows, Geoff, Accounting and Business Law, and Clive Morton, co-author, after receiving award for their book ‘The Canecutters’
No full textThis record was harvested from a previous catalogue system and will be withdrawn in 2025. Information in this record may be superseded or incomplete. Visit this record in UMA's new catalogue at: https://archives.library.unimelb.edu.au/nodes/view/283960Burrows, Geoff, Accounting and Business Law, and Clive Morton, co-author, after receiving award for their book ‘The Canecutters’286830
Item: [2003.0003.00938] "Photograph - Burrows, Geoff, Accounting and Business Law, and Clive Morton, co-author, after receiving award for their book ‘The Canecutters’
Photograph - Burrows, Geoff, Accounting and Business Law, and Clive Morton, co-author, who won award for their book ‘The Canecutters’
No full textThis record was harvested from a previous catalogue system and will be withdrawn in 2025. Information in this record may be superseded or incomplete. Visit this record in UMA's new catalogue at: https://archives.library.unimelb.edu.au/nodes/view/283981Burrows, Geoff, Accounting and Business Law, and Clive Morton, co-author, who won award for their book ‘The Canecutters’ 27 Feb 1987286851
Item: [2003.0003.00959] "Photograph - Burrows, Geoff, Accounting and Business Law, and Clive Morton, co-author, who won award for their book ‘The Canecutters’
Temperature-induced gelation of emulsions stabilised by responsive copolymers: A rheological study
No full textThe steady-state and dynamic rheological properties of 1-bromohexadecane-in-water emulsions stabilised by responsive poly(N-isopropylacrylamide)-co-poly(ethyleneglycol methacrylate) (poly(NIPAM-co-PEGMa)) copolymer have been investigated. The data were compared to measurements performed using dilute and concentrated poly(NIPAM-co-PEGMa) copolymer solutions. These solutions exhibit viscosities that decrease and increase, respectively, with increasing temperature. The increase in viscosity for the concentrated solution is attributed to transient network formation. The presence of dispersed oil droplets (volume fraction = 0.30) in the emulsion causes temperature-induced gelation even though the copolymer concentration in the aqueous phase is such that transient network formation due to overlapping copolymer chains throughout the aqueous phase does not occur. Dynamic rheological data confirmed gelation for the emulsions at elevated temperatures. Emulsion gelation is attributed to flocculation between neighbouring droplets. The adhesive droplets form a network that entraps the aqueous phase. The strength of the network increased with increasing temperature. To our knowledge these data represent the first comprehensive rheological investigation of reversible, temperature-induced gelation for an oil-in-water emulsion.Andrew Y. C. Koh, Clive Prestidge, Igor Ametov and Brian R. Saunder
A topical hydrogel with deferiprone and gallium-protoporphyrin targets bacterial iron metabolism and has antibiofilm activity
No full textAccepted manuscript posted online 10 April 2017Many infectious diseases are associated with multidrug-resistant (MDR) bacteria residing in biofilms that require high antibiotic concentrations. While oral drug delivery is frequently ineffective, topical treatments have the potential to deliver higher drug concentrations to the infection site while reducing systemic side effects. This study determined the antibiofilm activity of a surgical wound gel loaded with the iron chelator deferiprone (Def) and the heme analogue gallium-protoporphyrin (GaPP), alone and in combination with ciprofloxacin. Activity against MDR Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Acinetobacter johnsonii biofilms was assessed in the colony biofilm and artificial wound model by enumeration of CFU and correlative light/electron microscopy. While Staphylococcus biofilms were equally susceptible to GaPP and Def-GaPP gels (log10 reduction of 3.8 and 3.7, respectively), the Def-GaPP combination was crucial for significant activity against P. aeruginosa biofilms (log10 reduction of 1.3 for GaPP and 3.3 for Def-GaPP). When Def-GaPP gel was combined with ciprofloxacin, the efficacy exceeded the activity of the individual compounds. Def-GaPP delivered in a surgical wound gel showed significant antibiofilm activity against different MDR strains and could enhance the gel's wound-healing properties. Moreover, Def-GaPP indicated a potentiation of ciprofloxacin. This antibiofilm strategy has potential for clinical utilization as a therapy for topical biofilm-related infections.Katharina Richter, Nicky Thomas, Jolien Claeys, Jonathan McGuane, Clive A. Prestidge, Tom Coenye, Peter-John Wormald, Sarah Vreugd
A Liposome-Micelle-Hybrid (LMH) Oral Delivery System for Poorly Water-Soluble Drugs: Enhancing Solubilisation and Intestinal Transport
No full textA novel liposome-micelle-hybrid (LMH) carrier system was developed as a superior oral drug delivery platform compared to conventional liposome or micelle formulations. The optimal LMH system was engineered by encapsulating TPGS micelles in the aqueous core of liposomes and its efficacy for oral delivery was demonstrated using lovastatin (LOV) as a model poorly soluble drug with P-gp (permeability glycoprotein) limited intestinal absorption. LOV-LMH was characterised as unilamellar, spherical vesicles encapsulating micellar structures within the interior aqueous core and showing an average diameter below 200 nm. LMH demonstrated enhanced drug loading, water apparent solubility and extended/controlled release of LOV compared to conventional liposomes and micelles. LMH exhibited enhanced LOV absorption and transportation in a Caco-2 cell monolayer model of the intestine by inhibiting the P-gp transporter system compared to free LOV. The LMH system is a promising novel oral delivery approach for enhancing bioavailability of poorly water-soluble drugs, especially those presenting P-gp effluxes limited absorption
Capturing and Quantifying Particle Transcytosis with Microphysiological Intestine-on-Chip Models
No full textData source: Supporting information, https://doi.org/10.1002/smtd.202200989Understanding the intestinal transport of particles is critical in several fields ranging from optimizing drug delivery systems to capturing health risks from the increased presence of nano- and micro-sized particles in human environment. While Caco-2 cell monolayers grown on permeable supports are the traditional in vitro model used to probe intestinal absorption of dis-solved molecules, they fail to recapitulate the transcytotic activity of polar-ized enterocytes. Here, an intestine-on-chip model is combined with in silico modeling to demonstrate that the rate of particle transcytosis is ≈350× higher across Caco-2 cell monolayers exposed to fluid shear stress compared to Caco-2 cells in standard “static” configuration. This relates to profound phe-notypical alterations and highly polarized state of cells grown under mechan-ical stimulation and it is shown that transcytosis in the microphysiological model is energy-dependent and involves both clathrin and macropinocytosis mediated endocytic pathways. Finally, it is demonstrated that the increased rate of transcytosis through cells exposed to flow is explained by a higher rate of internal particle transport (i.e., vesicular cellular trafficking and baso-lateral exocytosis), rather than a change in apical uptake (i.e., binding and endocytosis). Taken together, the findings have important implications for addressing research questions concerning intestinal transport of engineered and environmental particles.Ludivine C. Delon, Matthew Faria, Zhengyang Jia, Stuart Johnston, Rachel Gibson, Clive A. Prestidge, and Benjamin Thierr
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