1,721,020 research outputs found
Detection of Helicobacter in gastric washing of cats
No full textThe aim of the current study was to evaluate the efficacy of gastric washing for detecting the presence of Helicobacter spp. in feline gastric mucus. Gastric fluids were collected from 11 cats undergoing routine surgical procedures. The fluids were centrifuged and the pellets were subjected to cytological examination by May-Grünwald/Giemsa (MGG) staining and testing by polymerase chain reaction (PCR) for the presence of Helicobacter after DNA extraction. Helicobacter spp. were detected in 8 of 11 samples by MGG staining and in 10 of 11 samples by PCR. Gastric washing proved to be a useful sampling technique and a valuable alternative to the gold-standard method of gastric biopsy, which has the risk of missing colonized spots in the case of patchy Helicobacter colonization. Gastric washing, a noninvasive way of sampling, could be applied to cats undergoing general anesthesia for different causes. Detection of Helicobacter in cats could allow epidemiological investigations, and sequencing of samples could assist in assessing the distribution of various Helicobacter species
UNUSUAL CASE OF UTERINE STUMP PIOMETRA IN A CAT
No full textThis report describes an unusual case of uterine stump pyometra in a cat whose main clinical sign at presentation was abdominal straining. At the time of ovariohysterectomy, the surgeon reported that the uterine body had a purulent content. Nearly a month after the surgery the cat showed abdominal straining. The enlarged uterine stump, filled with purulent fluid, had caused a compression of the rectum and secondary intestinal sub-occlusion. Surgical revision consisted of draining the purulent content of the remnant of the uterine body and ablating as much of it as possible; checking of the ovarian pedicles revealed the presence of a small fragment of whitish tissue on the right side, which was shown to contain, by means of histological observation and immunohistochemical staining, ovarian tissue. Four months after surgical revision the queen did not show any pathological signs and 1 year later she is still in good healt
Prevalence of Chlamydophila felis and feline herpesvirus 1 in cats with conjunctivitis in northern Italy.
No full textImmunofluorescence staining for the detection of immunoglobulins and complement (C3) in dogs with renal disease.
No full textRenal cortical biopsies from 74 dogs with different degrees of renal failure were studied by immunofluorescence to assess the frequency and extent of the deposition of immunoglobulins G, M and A (IgG, IgM, IgA) and complement C3. The dogs were divided into two groups on the basis of their clinical signs, and standard histological and electron microscopical examinations, according to whether their disease was an immune-mediated nephropathy (IMN) or a non-immune-mediated nephropathy (NIMN). in the dogs with an IMN there was strong immunofluorescence due to IgG in the mesangium and the glomerular basement membrane and to IgM in the mesangium. The mechanism of immune complex trapping in the glomerulus also resulted in positive reactions to IgM in the dogs with an NIMN
DETECTION OF ELICOBACTER CANDIDATUS SUIS BY PCR IN OESOPHAGOGASTRIC ULCERS OF SWINE IN ITALY
No full textThe aim of this study was to evaluate by PCR the presence of Helicobacter spp. in gastric mucus from the fundic region of the stomach and to investigate its role in oesophagogastric ulcers in swine bred and regularly slaughtered in Piedmont (Northern Italy). Stomachs from 595 regularly slaughtered swine were subjected to gross pathological examination in order to evaluate the presence of gastric ulcers (revealed in 75 cases, 12.6%). Histopathological examination was performed to better characterise erosions and ulcers. DNA extracted from gastric mucus collected from all the ulcer-affected and from 25 normal stomachs was submitted to PCR using Helicobacter spp. 16S rRNA gene target primers. Sixty-three percent (47/75) of the affected stomachs was positive as well as 24% (6/25) of the non-affected ones. Sequence analysis from 5 positive samples showed 99% homology with Helicobacter candidatus suis 16S ribosomal RNA gen
CONTROLLO DELLE INFEZIONI DA SALMONELLA NEL SUINO: STRATEGIE VACCINALI CONTROLLING SALMONELLA INFECTIONS IN PIGS: VACCINATION PROTOCOLS
Full text linkAbstract
first results.
Salmonella sp. is responsible of human infections due to consumption of contaminated
pork products. Carrier pigs introduce bacteria in the slaughter process. Italian situation is
characterized by a high percentage of infected farms, and vaccination could have a decisive
role in controlling infection. Unfortunately, none vaccine, licensed for swine, is available in
Italy. The aim of our work is to describe data related to eficacy and safety of an attenuated
S.Typhimurium vaccine, tested in experimental conditions, and an inactivated autogenous
vaccine, tested in ield conditions. Attenuated strain is safe and protective, limiting fecal
spread and organs colonization by virulent S.Typhimurium and it is also effective when intramuscularly administered or challenge is performed with S.Choleraesuis. Inactivated
autogenous vaccine was administered in pregnant sows and their piglets of two farms.
The results of this trial conirmed that sows vaccination, therefore antibody transfer with
colostrum, is more protective than direct piglets vaccination
16s rrna sequencing analysis of the gut microbiota in broiler chickens prophylactically administered with antimicrobial agents
Full text linkIn poultry production, gut microbiota (GM) plays a pivotal role and influences different host functions related to the efficiency of production performances. Antimicrobial (AM) use is one of the main factors affecting GM composition and functions. Although several studies have focused their attention on the role of AMs as growth promoters in the modulation of GM in broilers, the consequences of higher AM concentrations administered during prophylactic treatments need to be better elucidated. For this purpose, 16S rRNA gene sequencing was performed to evaluate the impact of different prophylactic AM protocols on the composition and diversity of the broiler GM. Diversity analysis has shown that AM treatment significantly affects alpha diversity in ileum and beta diversity in both ileum and caecum. In ileal samples, the Enterobacteriaceae family has been shown to be particularly affected by AM treatments. AMs have been demonstrated to affect GM composition in broiler. These findings indicate that withdrawal periods were not enough for the restoral of the original GM. Further studies are needed for a better elucidation of the negative effects caused by an altered GM in broilers
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