6,967 research outputs found
TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation
No full textThe interaction between TACC3 (transforming acidic coiled coil protein 3) and the microtubule polymerase ch-TOG (colonic, hepatic tumor overexpressed gene) is evolutionarily conserved. Loading of TACC3–ch-TOG onto spindle microtubules requires the phosphorylation of TACC3 by Aurora-A kinase and the subsequent interaction of TACC3 with clathrin to form a microtubule binding surface. Whether there is a pool of TACC3–ch-TOG that is independent of clathrin in human cells, and what is the function of this pool, are open questions. Here, we report that TACC3 is recruited to the plus-ends of microtubules by its association with ch-TOG and that this pool is independent of phosphorylation and binding to clathrin. The plus-end binding of TACC3–ch-TOG persists in interphase and we propose that one cellular function of TACC3–ch-TOG is to modulate cell migration. We also describe the distinct subcellular pools of TACC3, ch-TOG and clathrin. TACC3 is often described as a centrosomal protein, but we show that there is no significant population of TACC3 at centrosomes. The delineation of distinct protein pools reveals a simplified view of how these proteins are organized and controlled by post-translational modification
Dopant loss mechanism in n+/p germanium junctions during rapid thermal annealing
No full text10.1149/1.2073048Journal of the Electrochemical Society15212G895-G899JESO
A study of the acid lability and acute toxicity of dimethoxymethane in rats
No full textThe pH stability and acute oral toxicity of dimethoxymethane (DMM) in rats were studied, In in vitro studies, DMM was found to be stable in artificial gastric juice at pH 2.5 and above but readily hydrolyzed at pH2 and below, with 1 mole of DMM producing approximately 1 mole of formaldehyde and 2 moles of methanol. DMM incubated in sealed, excised stomachs appeared to equilibrate with the external incubation fluid over a 24-hour period without significant hydrolysis, In the toxicity study, three groups of female rats (n = 5) were administered, by gavage, phosphate-buffered saline (control), DMM (31 mmoles/kg body weight [bw]/day), or methanol (MeOH) (30 mmoles/kg bw/day), respectively, for 3 consecutive days and then sacrificed on day 5, Transient ataxia lasting about 30 minutes was observed in animals following DMM administration, increased urine volume was observed in the DMM group at the end of days 4 and 5, Markedly elevated level of formic acid was present in the urine of the MeOH group at days 1, 2, and 3, but not in the DMM group, Urinary ascorbic acid levels were increased In the DMM group on days 1, 2 and 3, and returned to baseline level at days 4 and 5, Microscopic examination detected no abnormal histological changes in the liver and kidneys of MeOH- or DMM-treated animals, No treatment effects were observed on the following endpoints: organ weights (liver, heart, thymus, kidneys), serum corticosterone, serum chemistry profile, hematology, hepatic phase 1 and phase 2 mixed-function oxidase activities, hepatic and serum thiobarbituric acid, hepatic glutathione, urinary protein, and urinary N-acetylglucosaminidase activity, These observations suggested that hydrolysis of DMM in the stomach required a gastric acidity below pH 2,5, At higher DW, intact DMM readily penetrated the stomach and therefore likely reached the circulation and other body tissues. There was no significant hydrolysis of orally administered DMM, and the acute effects were limited to transient ataxia and biochemical responses in the liver.PT: J; CR: *ACGIH, THRESH VAL TLVS CHEM *CCAC, 1993, GUID CAR US EXP AN *HSDB, 1998, HSDB *IARC, 1995, IARC MON EV CARC RIS, V62, P217 *US EPA, 1998, CHEM HAZ DAT AV STUD *USP, 1995, US PHARM, V23 BENINI L, 1995, GUT, V36, P825 BURCHELL B, 1981, METHOD ENZYMOL, V77, P169 BURKE MD, 1985, BIOCHEM PHARMACOL, V34, P3337 CHIVERTON SG, 1989, ALIMENT PHARM THERAP, V3, P403 CHU I, 1992, ORGANOHALOGEN COMPOU, V10, P17 DODGE L, 1994, NTIDDE94006949 GARGAS ML, 1989, TOXICOL APPL PHARM, V98, P87 GRADY S, 1986, J ANAL TOXICOL, V10, P1 HABIG WH, 1974, J BIOL CHEM, V249, P7130 JONES AW, 1993, CLIN CHEM, V39, P1837 KAO CH, 1998, J NUCL MED, V39, P1798 KONTUREK JW, 1993, SCAND J GASTROENTERO, V28, P401 LUBET RA, 1985, MUTAT RES, V142, P127 MAHE S, 1995, GASTROEN CLIN BIOL, V19, P20 MARICQ MM, 1998, 216 AM CHEM SOC NAT, V43, P578 MCLAUCHLAN G, 1989, GUT, V30, P573 MCMARTIN KE, 1980, AM J MED, V68, P414 POON R, 1992, P 4 ANN M INT SOC ST, P146 POON R, 1994, J BIOCHEM TOXICOL, V9, P297 POON R, 1995, INHAL TOXICOL, V7, P1195 ROGERS JM, 1993, TERATOLOGY, V47, P175 SAKANASHI TM, 1996, TERATOLOGY, V54, P198 SCHENKMAN JB, 1967, MOL PHARMACOL, V3, P113 TOMILINA LA, 1984, GIG TR PROF ZABOL, V6, P27 VANHERCK H, 1991, LAB ANIM, V25, P325 VINET B, 1987, CLIN CHEM, V33, P2204 YAGI K, 1982, LIPID PEROXIDE BIOL, P232; NR: 33; TC: 4; J9: INT J TOXICOL; PG: 7; GA: 331DTSource type: Electronic(1
Electrical evaluation of laser annealed junctions by Hall measurements
No full textHall measurements performed on laser annealed ultrashallow ion implanted p-type layers on n-type substrates over a wide temperature range showed expected p-type characteristics at low temperature but unexpected n-type behaviour at higher temperature. Samples annealed with lower laser energy fluences displayed the unexpected n-type characteristics over a wider temperature range compared to those annealed at higher energy fluences. These results can be explained by the increased contribution of the n-type substrate to the Hall readings, made possible by a more leaky junction, when the implant damage is inadequately removed. Thus, Hall measurements can be proposed as a quick evaluation tool of the integrity of laser annealed junctions without the need of device fabrication or high-resolution transmission electron microscopy. (C) 2004 Elsevier B.V. All rights reserved
Boron profile narrowing in laser-processed silicon after rapid thermal anneal
No full text10.1149/1.1633764Journal of the Electrochemical Society1511G80-G83JESO
Les diminutifs basques avec ch
No full textSe presentan formaciones similares a los diminutivos vascos con "ch" en España y América latina. Se dan ejemplosThe author introduces similar formations to the Basque diminutive "ch" in Spain and Latin America. Examples are provide
COUPLING OF THE C-H STRETCH TO LARGE-AMPLITUDE TORSION AND INVERSION MOTIONS: COMPARISON OF CHCH, CHOH AND CHNH
No full textAuthor Institution: Department of Polymer Science and Department of Chemistry, The University of Akron; Department of Chemistry, The University of Akron, OH 44325In each of the title molecules, torsional and inversion tunneling occurs between six equivalent minima. Coupling of these degrees of freedom to the CH stretch occurs via variation of the C-H stretching force constants as a function of the torsional () and inversion () angles. Maps of the couplings have been computed at the MP2/6-311++G(3df,2p) level. Both the single bond CH stretch force constants and the bilinear couplings between CH bonds are presented as a function of and . Although the torsional barriers differ by more than a factor of 20, the torsion-inversion-vibration coupling patterns are very similar for CHNH and CHCH. On the other hand, the torsion-inversion-vibration coupling in the charged species CHOH is much weaker
Reply to the Ch. Lagrange’s note
No full textThe author answers to a Ch. Lagrange’s note who refutes his theory on the diurnal nutation.L’auteur répond à une note de Ch. Lagrange qui réfute sa théorie sur la nutation diurne
NATURE OF TORSION-INVERSION COUPLING IN CHNH, CHOH AND CHCH
No full textAuthor Institution: Department of Chemistry, The University of Akron, OH; 44325-3601Two-dimensional torsion-inversion surfaces for methylamine, protonated methanol and ethyl radical were calculated and fit to a function containing a polynomial in the inversion angle() and trigonometric functions of the torsional angle(). Calculations were done at the B3LYP, MP2, and CCSD(T) levels with the 6-311++G(d,p) and 6-311++G(3df, 2p) basis sets and partial optimization. CHNH, CHOH and CHCH have G symmetry with 6-equivalent minima which are located by the various calculations at inversion angles 6.5 to 11; 42 to 45.5 and 52.5 to 55 degrees respectively on either side of planar. The three molecules have very different barriers to inversion ranging from no barrier for CHCH to 838 cm for CHOH to 1837 cm for CHNH. The dominant torsion-inversion coupling term in all cases has the form
Note about Mr. Ch. Lagrange’s recent communication
No full textThe author establishes a note about Mr. Ch. Lagrange’s communication on the Eulerian nutation period.L’auteur établit une note au sujet d’une communication de Ch. Lagrande sur la période de la nutation eulérienne
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