1,894,979 research outputs found
Anthropometric and physiological changes in elite female water polo players during a training year
No full textThe aim of the current study was to monitor physiological and anthropometric characteristics of elite female water polo players within the periodized training year. Fourteen subjects participated in the current study. However, only six subjects (Mean ± SD: Age 22.8 ± 3.7 years, Height 171.0 ± 10.8 cm, Body Mass 66.3 ± 4.7 kg) completed all sessions and thus were used for subsequent analyses. Subjects undertook testing in the general preparation, specific preparation and competition phases, with the final session being during the peaking component of the competition phase. Laboratory-based physiological measurements comprised peak oxygen uptake, anaerobic power, leg power, strength and flexibility, while anthropometric measurements included body fat percentage. Sport-specific tests involved the Multistage Swimming Shuttle Test (MSST) and the 30-second crossbar jumps. A repeated measures ANOVA revealed significant differences between testing sessions for body mass (F3,15 = 4.025, P = 0.028), body fat (F3,15 = 9.194, P = 0.001), MSST (F3,15 = 5.050, P = 0.017) and crossbar jumps (F2,10= 16.034, P = 0.001). No statistically significant differences were found for any other variables. The results of the study suggest that changes in anthropometric characteristics and performance parameters of elite female water polo players over a periodized training year occur with no changes in laboratory-based physiological measurements
Comparison of two anaerobic water polo-specific tests with the Wingate test
Get PDFThe purpose of the current study was to compare 2 water polo–specific tests—the 14 3 25-m swims (SWIM) and the 30-second crossbar jumps (30CJ)—with a laboratory-based test of anaerobic power, the Wingate Anaerobic Test (WAnT). Thirteen elite women’s water polo players (mean 6 SD: age 22.0 6 4.4 years, height 168.7 6 7.9 cm, body mass 65.9 6 6.1 kg, body fat 23.6 6 3.5 %, maximum oxygen uptake 51.4 6 4.5 mlkg21min21) participated in the study. The SWIM involved 14 repeated ‘‘all-out’’ sprints every 30 seconds. Swimming time was recorded, and sprint velocity, mean velocity (Vmean), and the gradient of the linear regression equation (GRADIENT) were calculated. The 30CJ involved repeated in-water water polo jumps and touching the goal crossbar with both hands. The number of touches in 30 seconds was recorded. Additionally, the subjects completed a 30-second WAnT, and mean power (Mp) and fatigue index (FI) were calculated. Kendall tau (t) rank correlation was used to examine for correlation between ranks. Significance level was set at p # 0.05. No significant correlation was found between any of the measures of the WAnT and the 2 sport-specific tests. It was suggested that the WAnT may not be an appropriate evaluation tool for anaerobic power assessment of water polo players, stressing the importance of sport-specific tests
STIL binding to Polo-box 3 of PLK4 regulates centriole duplication
Get PDFPolo-like kinases (PLK) are eukaryotic regulators of cell cycle progression, mitosis and cytokinesis; PLK4 is a master regulator of centriole duplication. Here, we demonstrate that the SCL/TAL1 interrupting locus (STIL) protein interacts via its coiled-coil region (STIL-CC) with PLK4 in vivo. STIL-CC is the first identified interaction partner of Polo-box 3 (PB3) of PLK4 and also uses a secondary interaction site in the PLK4 L1 region. Structure determination of free PLK4-PB3 and its STIL-CC complex via NMR and crystallography reveals a novel mode of Polo-box-peptide interaction mimicking coiled-coil formation. In vivo analysis of structure-guided STIL mutants reveals distinct binding modes to PLK4-PB3 and L1, as well as interplay of STIL oligomerization with PLK4 binding. We suggest that the STIL-CC/PLK4 interaction mediates PLK4 activation as well as stabilization of centriolar PLK4 and plays a key role in centriole duplication
Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis
Get PDFWe have investigated the function of mitotic kinesin-like protein (MKlp) 2, a kinesin localized to the central spindle, and demonstrate that its depletion results in a failure of cleavage furrow ingression and cytokinesis, and disrupts localization of polo-like kinase 1 (Plk1). MKlp2 is a target for Plk1, and phosphorylated MKlp2 binds to the polo box domain of Plk1. Plk1 also binds directly to microtubules and targets to the central spindle via its polo box domain, and this interaction controls the activity of Plk1 toward MKlp2. An antibody to the neck region of MKlp2 that prevents phosphorylation of MKlp2 by Plk1 causes a cytokinesis defect when introduced into cells. We propose that phosphorylation of MKlp2 by Plk1 is necessary for the spatial restriction of Plk1 to the central spindle during anaphase and telophase, and the complex of these two proteins is required for cytokinesis
COORDENADOR DE POLO: UMA DISCUSSÃO ACERCA DOS CONHECIMENTOS NECESSÁRIOS À UMA BOA GESTÃO
Get PDFO presente trabalho tem como objetivo apresentar e discutir uma série de competências complementares às exigidas legalmente que auxiliarão a figura do Coordenador de Polo no processo de gerenciamento de um Polo de Apoio Presencial. Entende-se que, apenas com os pré-requisitos mínimos exigidos por lei, que privilegiam os aspectos acadêmicos em detrimento de conhecimentos de práticas de gestão, os Coordenadores de Polo podem não se mostrar preparados para o exercício de suas funções. Desta forma, num cenário de expressivo crescimento de cursos de Educação a Distância (EaD), não tendo as competências técnicas necessárias, os coordenadores de Polos poderão não suprir as necessidades do Polo, conseqüentemente, não atenderão as expectativas, demandas e necessidades dos alunos, professores e da própria comunidade. Sugere-se, então, alguns processos de gestão que poderão auxiliá-los em suas tarefas, a saber: gestão estratégica, gestão da infra-estrutura, gestão de pessoas, gestão de processos, gestão de marketing. Os pré-requisitos básicos para a contratação de um coordenador de Polo não exigem, a certificação dos conhecimentos nas áreas de gestão, mas a necessidade imposta pelo meio, cada vez mais requer tais conhecimentos
Polo Event- Polo Cup Packet
No full textInformation packet for polo event for U of U College of Pharmacy, June 5, 2010
Polo Event- Small Book
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Polo Event- Small Handouts
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Polo Event- Large Book
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Polo Event- Ticket Holder
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