1,964 research outputs found
L'"ERETICO" PIERO GOBETTI E LE TRE "DIMENSIONI" DELLA STORIA DEL RISORGIMENTO
No full textSOMMARIO: 1. Premessa - 2. La dimensione “teorica” - 3. La dimensione “pratica” - 4. La dimensione “personale” - 5. Conclusione.The “heretic” Piero Gobetti and the three “dimensions” of the history of the RisorgimentoABSTRACT: Piero Gobetti’s “the heresy of the Risorgimento” allows to distinguish three “points of view” of the political and cultural experience of the “heretical” Gobetti, the “theoretical” one, the “practical” one and “personal” one. Three “profiles” that realized a systematic dimension, in the relationship of the Risorgimento with the “Liberal Revolution”, according to a research perspective that has never been fully analyzed. A reflection that highlights the “drama of the Risorgimento”, the “programmatic formulas” of “The Liberal Revolution” and Piero Gobetti\u27s “clarity of authentic feelings” which moves on the “ground of heresy” with his “choice” of life
Returning culture to peacebuilding : contesting the liberal peace in Sierra Leone
No full textThis thesis investigates the advantages and limitations of applying culture to the analysis of violent conflict and peacebuilding, with a particular focus on liberal peacebuilding in Sierra Leone. While fully aware of the critique of the concept of culture in terms of its uses for the production of difference and ‘otherness,’ it also seeks to respond to the critique of liberal peacebuilding on the account of its low sensitivity towards local culture, which allegedly undermines the peace effort. After a careful examination of the terms of discussion about culture enabled by theoretical approaches to conflict in Chapter 2, the thesis presents a theoretical framework for the analysis of cultural aspects of conflict and peace based on the processes and effects of meaning-generation (Chapter 3), developing the conceptual apparatus and vocabulary for the subsequent empirical study. Instead of bracketing out the recursive nature of cultural theorising, the developed approach embraces the recursive dynamics which arise as a result of cultural ‘embeddedness’ of the analyst and the processes which s/he seeks to elucidate, mirroring similar dynamics in the cultural production of meaning and knowledge. The framework of ‘embedded cultural enquiry’ is then used to analyse the practices of liberal peacebuilding as a particular culture, which shapes the interaction of the liberal peace with its ‘subjects’ and critics as well as framing its reception of the cultural problematic generally (Chapter 4). The application of the analytical framework to the case study investigates the interaction between the liberal peace and ‘local culture,’ offering an alternative reading of the conflict and peace process in Sierra Leone (Chapter 5). The study concludes that a greater attention to cultural meaning-making offers a largely untapped potential for peacebuilding, although any decisions with regard to its deployment will inevitably be made from within an inherently biased cultural perspective
Erratum to: Reasoning with infinite stable models [Artificial Intelligence 156 (1) (2004) 75-111]
No full textAbstractTheorem 16 in [Piero A. Bonatti, Reasoning with infinite stable models, Artificial Intelligence 156 (1) (2004) 75–111] states that ground skeptical and credulous inferences under the stable model semantics are decidable when the given normal logic program is finitary. Giovanni Criscuolo and Nicola Leone independently observed in personal communications that the proof of this theorem relies on an unproved assumption that—at the best of our current knowledge—might turn out to be false. In this note we correct Theorem 16 by adding the set of odd-cyclic atoms to the inputs of the computation, and argue that this change has no impact on the current applications of the theory of finitary programs
Gian Piero Brunetta, Il ruggito del Leone. Hollywood alla conquista dell’impero dei sogni nell’Italia di Mussolini
No full textL’ouvrage de Gian Piero Brunetta, récemment publié chez Marsilio, vient compléter une trilogie autour du cinéma comme véhicule d’une histoire et d’un rêve collectif et populaire entamée avec Buio in sala (1989) et poursuivie avec Il viaggio dell’icononauta (1997). Étroitement lié au premier pour son analyse du rapport entre spectateur et film – entendu dans sa double valeur d’œuvre artistique et produit d’une industrie culturelle – Il ruggito del Leone (« le rugissement du lion » – de la MGM)..
Gian Piero Brunetta, Il ruggito del Leone. Hollywood alla conquista dell’impero dei sogni nell’Italia di Mussolini
No full textL’ouvrage de Gian Piero Brunetta, récemment publié chez Marsilio, vient compléter une trilogie autour du cinéma comme véhicule d’une histoire et d’un rêve collectif et populaire entamée avec Buio in sala (1989) et poursuivie avec Il viaggio dell’icononauta (1997). Étroitement lié au premier pour son analyse du rapport entre spectateur et film – entendu dans sa double valeur d’œuvre artistique et produit d’une industrie culturelle – Il ruggito del Leone (« le rugissement du lion » – de la MGM)..
Continuous and Discontinuous Approaches to Study FAD Synthesis and Degradation Catalyzed by Purified Recombinant FAD Synthase or Cellular Fractions
No full textRiboflavin, or vitamin B2, is the precursor of flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), essential redox (and sometimes non-redox) cofactors of a large number of flavoenzymes involved in energetic metabolism, protein folding, apoptosis, chromatin remodeling, and a number of other cell regulatory processes.The cellular and subcellular steady-state concentrations of flavin cofactors, which are available for flavoprotein biogenesis and assembly, depend on carrier-mediated transport processes and on coordinated synthesizing/destroying enzymatic activities, catalyzed by enzymes whose catalytic and structural properties are still matter of investigation.Alteration of flavin homeostasis has been recently correlated to human pathological conditions, such as neuromuscular disorders and cancer, and therefore we propose here protocols useful to detect metabolic processes involved in FAD forming and destroying.Our protocols exploit the chemical-structural differences between riboflavin, FMN , and FAD , which are responsible for differences in the spectroscopic properties (mainly fluorescence) of the two cofactors (FMN and FAD); therefore, in our opinion, when applicable measurements of fluorescence changes in continuo represent the elective techniques to follow FAD synthesis and degradation. Thus, after procedures able to calibrate flavin concentrations (Subheading 3.1), we describe simple continuous and rapid procedures, based on the peculiar optical properties of free flavins, useful to determine the rate of cofactor metabolism catalyzed by either recombinant enzymes or natural enzymes present in cellular lysates/subfractions (Subheading 3.2).Fluorescence properties of free flavins can also be useful in analytical determinations of the three molecular flavin forms, based on HPLC separation, with a quite high sensitivity. Assaying at different incubation times the molecular composition of the reaction mixture is a discontinuous experimental approach to measure the rate of FAD synthesis/degradation catalyzed by cell lysates or recombinant FAD synthase (Subheading 3.3). Continuous and discontinuous approaches can, when necessary, be performed in parallel
Purification of Recombinant Human 6His-FAD Synthase (Isoform 2) and Quantitation of FAD/Protein Monomer Ratio by UV-Vis Spectra
No full textHere we describe a protocol for a one-step purification of a soluble form of human FAD synthase (isoform 2; hFADS2), overexpressed as a 6-His-tagged fusion protein in Escherichia coli, with a yield of about 15 mg from 1 L of transformed bacterial culture.Following a desalting procedure, the protein is obtained in its FAD-bound form (about 0.8 molecules of FAD per 1 protein monomer). A simple method is also proposed here, for the rapid estimation of the [FAD ]/[protein monomer] ratio, starting from the typical flavoprotein spectrum of the purified protein fraction.The procedure described gives the protein at a quite high grade of purity (about 95%) and in its bifunctional (2.7.7.2/3.6.1.18) enzymatically active form, useful for further kinetical and molecular characterization
Author Visit inside Leone Cole Auditorium 1
No full textAn unidentified author was a special guest at Jacksonville State University in Spring 1968. Shown she stands on stage in Leone Cole Auditorium with unidentified students signing a book.https://digitalcommons.jsu.edu/lib-ac-histimg/20307/thumbnail.jp
Alteration of flavin homeostasis in neuromuscular disorders and cancer
No full textRiboflavin, otherwise known as vitamin B2, is an essential dietary component and represents the precursor of flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), the redox enzymatic cofactors required for mitochondrial terminal metabolism and for the functionality of mitochondrial respiratory chain. Protein folding, ROS production and defense, as well as redox epigenetics also depend on cellular supply of FAD.
FAD formation in different cells starts from riboflavin uptake, which occurs via specialized carrier-mediated processes which are supported by three specific members of the solute carrier family 52 (SLC52A), identified and named respectively RFVT1, RFVT2 and RFVT3. Once in the cell, riboflavin is converted to FMN by riboflavin kinase (RFK, EC2.7.1.26) and FMN is converted, in turn, to FAD by FAD synthase (FADS, EC 2.7.7.2), coded by human FLAD1 gene. Alternative splicing of the FLAD1 gene generates different hFADS isoforms, with different sub-cellular localization, most of them containing and a C-terminal 3-phosphoadenosine 5-phosphosulfate (PAPS) reductase domain, which per se performs the FAD synthase activity and a N-terminal molybdopterin binding (MPTb) domain, which could perform a FAD hydrolase activity.
Alterations of FAD synthesis and RFVT1 impairment have been correlated with a rare inherited neuro-muscular disorders, in some cases treatable with high doses of Riboflavin, named Multiple Acyl-CoA Dehydrogenase Deficiency (MADD, MIM: 231680). Here we describe a novel case of MADD caused by a truncating variant in FLAD1 resulting in a premature STOP codon in exon 2. Studies, carried out on patient fibroblasts, revealed a dramatic reduction in FADS protein level with corresponding reduction in FAD synthesis rate and in mitochondrial flavoenzyme amounts. The residual FAD activity measured could result from the expression of a novel isoform named 6, lacking MPTb domain, relevant for patient survival [1]. Immunoblotting analysis of RFVTs revealed a reduction in RFTV2 protein amount in fibroblasts of this patient, which is in line with the reduction of the total flavin cellular content observed by HPLC. The secondary defect of riboflavin transport rate in MADD patient, laying the foundations for explaining riboflavin therapy, links MADD to Brown-Vialetto-Van-Laere Syndrome (BVVLS, MIM: 21153; 614707) [2]. It is a neurological disorder characterized by bilateral sensoneural deafness, respiratory insufficiencies and progressive ponto-bulbar palsy, linked to mutations in RFVT2 and RFVT3 genes. A significant reduction in the intracellular levels of FMN and FAD was found in BVVLS patient fibroblasts grown in low extracellular riboflavin conditions [2].
We also pointed our attention on dysregulation of RFVTs expression linked to human colorectal [3] and some other types of cancer, generating a profound alteration of flavin cofactor homeostasis. Changing the level of RFVTs expression as a possible mean to reprogram cellular flavoproteome will be discussed
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