826 research outputs found
Regulation of Anopheles gambiae male accessory gland genes influences postmating response in female.
No full textIn Drosophila, the accessory gland proteins (Acps) secreted from the male accessory glands (MAGs) and transferred along with sperm into the female reproductive tract have been implicated in triggering postmating behavioral changes, including refractoriness to subsequent mating and propensity to egg laying. Recently, Acps have been found also in Anopheles, suggesting similar functions. Understanding the mechanisms underlying transcriptional regulation of Acps and their functional role in modulating Anopheles postmating behavior may lead to the identification of novel vector control strategies to reduce mosquito populations. We identified heat-shock factor (HSF) binding sites within the Acp promoters of male Anopheles gambiae and discovered three distinct Hsf isoforms; one being significantly up-regulated in the MAGs after mating. Through genome-wide transcription analysis of Hsf-silenced males, we observed significant down-regulation in 50% of the Acp genes if compared to control males treated with a construct directed against an unrelated bacterial sequence. Treated males retained normal life span and reproductive behavior compared to control males. However, mated wild-type females showed a ∼46% reduction of egg deposition rate and a ∼23% reduction of hatching rate (∼58% combined reduction of progeny). Our results highlight an unsuspected role of HSF in regulating Acp transcription in A. gambiae and provide evidence that Acp down-regulation in males leads a significant reduction of progeny, thus opening new avenues toward the development of novel vector control strategies.-Dottorini, T., Persampieri, T., Palladino, P., Baker, D. A., Spaccapelo, R., Senin, N., Crisanti, A. Regulation of Anopheles gambiae male accessory gland genes influences postmating response in female
Silencing of the Hsf gene, the transcriptional regulator of A. gambiae male accessory glands, inhibits the formation of the mating plug in mated females and disrupts their monogamous behaviour
No full textDiscovering the molecular factors that shape the mating behaviour and the fertility of the mosquito Anopheles gambiae, the principal vector of human malaria, is regarded as critical to better understand its reproductive success as well as for identifying new leads for malaria control measures. In A. gambiae mating induces complex behavioural and physiological changes in the females, including refractoriness to subsequent mating and induction of egg-laying. In other insects including Drosophila a group of proteins named Accessory gland proteins (Acps), produced by males and transferred with sperm to the female reproductive tract, have been implicated in this post-mating response. Although Acps represent a set of promising candidates for unravelling the mating physiology, their role in inducing behavioural changes in mated A. gambiae females remains largely unknown. In this work, we demonstrate that a down-regulation of a large fraction of Acp genes via silencing of the Acp regulating transcription factor Hsf, abolishes the formation of mating plug in mated females and fails to induce refractoriness of mated female to subsequent inseminations. A significant fraction of females mated to Hsf silenced males (66%) failed to receive the mating plug though seminal fluid had been transferred as documented by the presence of spermatozoa in the female sperm storage organ. Furthermore, nearly all females (95%) mated to HSF-silenced males were re-inseminated when exposed to males carrying EGPF marked sperm. Our findings provide evidence showing that Acp genes regulated by the transcription factor HSF play a key role in the function of the male accessory glands
CluGene: A Bioinformatics Framework for the Identification of Co-Localized, Co-Expressed and Co-Regulated Genes Aimed at the Investigation of Transcriptional Regulatory Networks from High-Throughput Expression Data
No full textThe full understanding of the mechanisms underlying transcriptional regulatory networks requires unravelling of complex causal relationships. Genome high-throughput technologies produce a huge amount of information pertaining gene expression and regulation; however, the complexity of the available data is often overwhelming and tools are needed to extract and organize the relevant information. This work starts from the assumption that the observation of co-occurrent events (in particular co-localization, co-expression and co-regulation) may provide a powerful starting point to begin unravelling transcriptional regulatory networks. Co-expressed genes often imply shared functional pathways; co-expressed and functionally related genes are often co-localized, too; moreover, co-expressed and co-localized genes are also potential targets for co-regulation; finally, co-regulation seems more frequent for genes mapped to proximal chromosome regions. Despite the recognized importance of analysing co-occurrent events, no bioinformatics solution allowing the simultaneous analysis of co-expression, co-localization and co-regulation is currently available. Our work resulted in developing and valuating CluGene, a software providing tools to analyze multiple types of co-occurrences within a single interactive environment allowing the interactive investigation of combined co-expression, co-localization and co-regulation of genes. The use of CluGene will enhance the power of testing hypothesis and experimental approaches aimed at unravelling transcriptional regulatory networks. The software is freely available at http://bioinfolab.unipg.it/
Regulation of Anopheles gambiae male accessory gland genes influences postmating response in female
No full textIn Drosophila, the accessory gland proteins (Acps) secreted from the male accessory glands (MAGs) and transferred along with sperm into the female reproductive tract have been implicated in triggering postmating behavioral changes, including refractoriness to subsequent mating and propensity to egg laying. Recently, Acps have been found also in Anopheles, suggesting similar functions. Understanding the mechanisms underlying transcriptional regulation of Acps and their functional role in modulating Anoph-eles postmating behavior may lead to the identification of novel vector control strategies to reduce mosquito populations. We identified heat-shock factor (HSF) binding sites within the Acp promoters of male Anoph-eles gambiae and discovered three distinct Hsf isoforms; one being significantly up-regulated in the MAGs after mating. Through genome-wide transcription analysis of Hsf-silenced males, we observed significant down-regulation in 50% of the Acp genes if compared to control males treated with a construct directed against an unrelated bacterial sequence. Treated males retained normal life span and reproductive behavior compared to control males. However, mated wild-type females showed a 46% reduction of egg deposition rate and a 23% reduction of hatching rate (58% combined reduction of progeny). Our results highlight an unsus-pected role of HSF in regulating Acp transcription in A. gambiae and provide evidence that Acp down-regulation in males leads a significant reduction of progeny, thus opening new avenues toward the development of novel vector control strategies.—Dottorini, T., Persampieri, T.
La descrizione delle risorse bibliografiche in linked data
No full textThe article is an appendix of the volume Come lavorare con Wikidata in biblioteca = How to work with Wikidata in the library, written by Alessandra Boccone and Tania Maio, published by Editrice Bibliografica in April 2021. In this article, after a brief introduction to Wikidata and to the construction of an element, the tables of the WikiProject Books and Periodicals are reported. They have been translated, revised and expanded by the author, for the benefit of librarians who wish to approach the creation of items of bibliographic interest, such as authors, monographs, journals, articles in linked open data. It ends with a brief reflection on the possibilities offered by Wikidata in the field of authority control
. 1096 Año 22 (2023) septiembre. El Tlacuache
No full textLas festividades dan cuenta de una diversidad de formas de relaciones sociales y de vinculación social con el territorio. Se ha destacado el papel que han tenido para generar momentos de convivencia y cooperación colectiva. Las personas brindan a la comunidad trabajo, dinero, productos y/o aportan capital social (es decir, facilitar algún trámite o el uso de algún espacio en la localidad).- Territorialidad y festividad. Celebración a Santo Domingo de Guzmán en Oaxtepec por Tania Alejandra Ramírez Rocha y Patricia Ramírez Ramírez.Jaripeo. Fotografía: Patricia Ramírez Ramírez, Oaxtepec, Morelos, 2023
El Tlacuache Núm. 921 (2020). 921 Año 19 (2020) febrero. El Tlacuache
No full textEl devenir de la infancia en México por Tania Alejandra Ramírez Rocha
El Tlacuache Núm. 926 (2020). 926 Año 19 (2020) marzo. El Tlacuache
No full text- La participación de la niñez en los huehuenches de Tlayacapan por Tania Ramírez Rocha y Patricia Ramírez Ramírez
The success of a collective mobilization
No full textTania Scacchetti, General Secretary of CGIL, introduces a comment on the collective agreement by Just Eat and Filt CIGL, Fit CISL, UIL Trasporti. The Author explains the
legislative and negotiating framework in which the last collective agreement was born and reconstructs the antecedents that led to its stipulation. She also discusses what she considers to be the salient parts of the contract, including the regulation of working time and rider compensation
#201| At Land's End: The Emergence Of Capitalist Relations On An Indigenous Frontier w/ Tania Li
No full textEpisode Notes: Learn more about Tania’s work at: taniamurrayli.wordpress.com, Learn more about ‘Land’s End’ and purchase a copy: bit.ly/2XZRO9b / bit.ly/2YPJeXA, The title card features a photo by Tania Li.
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EVERYTHING ELSE: linktr.ee/patterns.of.behavior,In this episode, I speak with Tania Li, Ph.D — Professor of Anthropology at the University of Toronto and the author of ‘Land’s End: Capitalist Relations on an Indigenous Frontier.’
In our era of globalized neoliberal capitalism, we tend to examine the emergence of capitalist economic and social relations among indigenous communities primarily as a result of overbearing external pressures, e.g. governments, nonprofit organizations, and multinational corporations (often in tandem). It is important, however, to recognize that while this is often the case, this view does not include the ways capitalism can emerge and take hold in far more subtle ways. As documented in ‘Land’s End,’ from 1990 to 2009 Tania conducted annual ethnographic research in the Lauje highlands of Sulawesi Indonesia, and bore witness to the indigenous population’s rapid adoption of the tree crop cocoa for cultivation, transitioning away from the more communally managed production of food crops, as had been done traditionally in these communities for generations. As Tania explains in this episode, the seemingly banal transformation the highlanders of this region experienced —transitioning from the communal production of food crops to the more privatized production of cocoa — not only produced capitalist relations among the Lauje, but did so with very minimal to non-existent pressures from outside institutions. How did this happen? What can we learn about nature of capitalism and its emergence from Tania’s profound ethnographic study, and how can we apply this knowledge to more adequately respond to the material conditions that produce these results? Tania and I discuss these questions and much more in this episode.
Tania Li, Ph.D is Professor of Anthropology at the University of Toronto and the author of several books, including ‘The Will to Improve,’ ‘Powers of Exclusion,’ and ‘Land’s End: Capitalist Relations on an Indigenous Frontier.’ Her current writing project is an ethnography, exploring the forms of social, political, cultural and economic life that emerge in Indonesia’s oil palm plantation zone
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