163,729 research outputs found
PERK-mediated phosphorylation of eIF2α causes elimination of <i>rer1</i> mutant cells.
No full text(A-D) Third-instar wing epithelium containing hs-FLP-induced MARCM clones (72 hrs AHS), of following genotypes, (A) UAS-GFP (rer1+/+), (B) UAS-GFP, rer1–/–, (C) UAS-GFP, rer1–/–+ UAS-PERK RNAi and (D) UAS-GFP, rer1–/–+ UAS-GCN2 RNAi, stained with the anti-p-eIF2α antibody. (E) Quantification of the p-eIF2α levels inside the GFP-positive clones with respect to the nearby GFP-negative control tissue in, A (N = 17 wing discs), B (N = 9 wing discs), C (N = 11 wing discs) and D (N = 10 wing discs). Statistical analysis was performed using the Ordinary one-way ANOVA with Tukey’s multiple comparison test (**** p(F) Quantification of the relative size of GFP-labeled clones area in; A (N = 29 wing discs), B, (N = 25 wing discs), C (N = 27 wing discs) and D (N = 29 wing discs). Statistical analysis was performed using the Ordinary one-way ANOVA with Tukey’s multiple comparison test (**** p(G-J) Third-instar wing epithelium containing hs-FLP-induced MARCM clones (72 hrs AHS) of following genotypes, (G) UAS-GFP (rer1+/+), (H) UAS-GFP, rer1–/–, (I) UAS-GFP, rer1–/–+ UAS-PERK RNAi and (J) UAS-GFP, rer1–/–+ UAS-GCN2 RNAi, stained with the anti-Dcp-1 antibody. (K) Quantification of the Dcp-1 in the GFP-labeled clones area in, G (N = 13 clones in 3 wing discs), H (N = 22 clones in 9 wing discs), I (N = 28 clones in 8 wing discs) and J (N = 8 clones in 6 wing discs). Statistical analysis was performed using the Ordinary one-way ANOVA with Tukey’s multiple comparison test (**** pS7 Fig.</p
Ralph J. Perk, the “New ethnicity”, and the making of urban ethnic republicans
No full textHistorians seeking to explain the late twentieth century rightward shift of urban ethnic whites have tended to ignore the shifting meaning and content of white ethnic identity in this transition, and the utility of these changes to conservative political discourse. This article, focusing on the ethnic strategies of the Republican mayor of Cleveland, Ralph Perk, seeks to illustrate the importance of the “New Ethnicity” of the 1970s, and its reconceptualization of white ethnicity as a series of “values”, in the making of urban ethnic Republicans. In doing so it reorients our understanding of Perk – the “Ethnic Mayor” – and places ethnicity at the heart of the conservative insurgency reshaping urban and national politics during this period
Cleveland Mayor Ralph J. Perk: Strong Leadership During Troubled Times
No full textThis book is a tribute to one of Cleveland’s greatest leaders. Ralph J. Perk embodied the quintessential 20th century politician. A dedicated administrator with both insight and foresight, he dedicated nearly fifty years to public service. During his six-year tenure as mayor, Cleveland went through extraordinary economic, political and social changes. The citizenry demanded strong leadership and Ralph Perk provided it. An optimistic leader with strong moral and religious convictions, he never walked away from a challenge.https://engagedscholarship.csuohio.edu/clevmembks/1017/thumbnail.jp
A short history of the European Association of Preventive Cardiology (EAPC)
No full textDe Backer G, Perk J, Wood D, et al. A short history of the European Association of Preventive Cardiology (EAPC). European Journal of Preventive Cardiology . 2022: zwac027.The history of the EAPC is closely related to the history of the European Society of Cardiology (ESC). The ESC decided at the turn of the century to overcome the splitting into 27 topic related Working Groups with great differences in size and activities and to create a new organizational concept based on Associations, each having an official ESC scientific journal and an annual ESC connected congress. The European Association for Cardiovascular Prevention and Rehabilitation was built on the fundaments of epidemiology and prevention, exercise physiology, cardiac rehabilitation and sports cardiology. The official journal of the Association was launched in 2003 and the first EuroPRevent Congress was held in Athens in 2006. During the following years, the different interests of the founding working groups came closer together, which resulted in a name change of the Association into "European Association of Preventive Cardiology" and of the journal into "European Journal of Preventive Cardiology". The name change marked the migration of Preventive Cardiology to centre stage in the ESC. This document summarizes how and from where the EAPC started and where it stands now. Published on behalf of the European Society of Cardiology. All rights reserved. © The Author(s) 2022. For permissions, please email: [email protected]
[Report to Chief J. E. Curry, by an unknown author #1]
No full textReport to Chief J. E. Curry, by an unknown author. The report contains a list of officers who gave depositions to the United States Attorney
[Report to Chief J. E. Curry, by an unknown author #2]
No full textReport to Chief J. E. Curry, by an unknown author. The report contains a list of officers who gave depositions to the United States Attorney
Co-localization of pERK/pRet and of pERK/IB4 immunoreactivity in the CA3 region following acute GDNF exposure.
No full text<p>(A-C): Low magnification fluorescence microphotographs of OHSCs immunolabeled for pERK (green), and pRet (red) in the CA3 region. (D-F) Higher magnification fluorescence images of the frame depicted in A-C displaying both pERK (green) and pRet (red) labelling in strata pyramidale of CA3 region. (G-I): Low magnification fluorescence microphotographs of OHSCs immunolabeled for IB4 (green), and pERK (red) in the CA3 region. (J-L): Higher magnification of frames depicted in G-I displaying cells double-labelled for both pERK and IB4 signals (arrows) and a cells positive for pERK only (arrowheads). Scale bars: 200 µm in A-C and G-I; 100 µm inD-F, 50 µm in J-L.</p
PERK-mediated expression of peptidylglycine α-amidating monooxygenase supports angiogenesis in glioblastoma
No full textPKR-like kinase (PERK) plays a significant role in inducing angiogenesis in various cancer types including glioblastoma. By proteomics analysis of the conditioned medium from a glioblastoma cell line treated with a PERK inhibitor, we showed that peptidylglycine α-amidating monooxygenase (PAM) expression is regulated by PERK under hypoxic conditions. Moreover, PERK activation via CCT020312 (a PERK selective activator) increased the cleavage and thus the generation of PAM cleaved cytosolic domain (PAM sfCD) that acts as a signaling molecule from the cytoplasm to the nuclei. PERK was also found to interact with PAM, suggesting a possible involvement in the generation of PAM sfCD. Knockdown of PERK or PAM reduced the formation of tubes by HUVECs in vitro. Furthermore, in vivo data highlighted the importance of PAM in the growth of glioblastoma with reduction of PAM expression in engrafted tumor significantly increasing the survival in mice. In summary, our data revealed PAM as a potential target for antiangiogenic therapy in glioblastoma
The Life & Times of Ralph J. Perk
No full textCleveland’s economic transformation from heavy manufacturing to health-related industries has not been easy. Many leaders are still discussing what course of action to follow. Studying successful policies from past politicians may afford new insight into how to handle this transition.
Effective politicians have always worked well with others; established achievable goals; and advanced community objectives. Mayor Ralph J. Perk was no exception. He initiated many broad-based programs while never forgetting the needs of the electorate.
A Web site dedicated to his life and work is intended to shed some new light on his political mastery during a very turbulent time in our city’s history. Many of his policies worked well then, perhaps they can be successfully replicated in today’s world.https://engagedscholarship.csuohio.edu/cmpex/1043/thumbnail.jp
Immunohistochemical single-labeling of pERK.
No full text<p><b>A–G:</b> schematic drawings of coronal brain sections (derived from the Paxinos rat brain atlas, 63) with grey squares corresponding to the location of the quantified brain areas (<b>A:</b> LSv, bregma +0.20; <b>B:</b> BSTmpm<b>,</b> bregma −0.80; <b>C</b>: PVNm and PVNl, bregma −1.80; <b>D:</b> HAA, bregma −2.80; <b>E:</b> MeApd, CeA, VHMvl<b>,</b> bregma −3.14; <b>F:</b> DRd, bregma −7.80; <b>G:</b> PAGvl, bregma −8.30); <b>H:</b> Average density of pERK-IR in non-aggressive (white bars, n = 7) compared with aggressive (grey bars, n = 7) adolescent (7–8 week old) female NAB rats. Data are means ± s.e.m; *P<0.05; <b>I–J:</b> Illustration of pERK-IR in the PVNmp (“mp”) and PVNlm (“lm”) of non-aggressive (left) and aggressive (right) individuals (3v = third ventricle); <b>K–L:</b> Illustration of pERK-IR in the HAA of non-aggressive (left) and aggressive (right) individuals.</p
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