1,435 research outputs found
Detection of Treponema pedis associated with digital dermatitis in Mediterranean buffalo (Bubalus bubalis)
No full textClinical outcomes compatible with digital dermatitis (DD) were observed in a second lactation, milking Mediterranean buffalo (MB). The animal was sharing the free stall with other 99 milking MBs separated by a fence from a pen harbouring 40 milking crossbreed dairy cows. The macroscopic lesion observed on the right rear limb was categorised as M4.1 stage and associated with a severe lameness (locomotion score 5). In the same farm, DD was also observed in 14/40 cows. Nested PCR analysis of genomic DNA isolated from the centre lesion's biopsy showed the presence of Treponema pedis. Sequence analysis of 494 bp of the PCR fragment revealed 100 per cent sequence identity to the T pedis-type strain, confirming the PCR result's specificity and for the first time the presence of the bacterium in MB. In order to establish the spread of the DD, a farm inspection was performed to identify risk factors and preventive strategies
DD-Pose: A large-scale Driver Head Pose Benchmark
No full textWe introduce DD-Pose, the Daimler TU Delft Driver Head Pose Benchmark, a large-scale and diverse benchmark for image-based head pose estimation and driver analysis. It contains 330k measurements from multiple cameras acquired by an in-car setup during naturalistic drives. Large out-of-plane head rotations and occlusions are induced by complex driving scenarios, such as parking and driver-pedestrian interactions. Precise head pose annotations are obtained by a motion capture sensor and a novel calibration device. A high resolution stereo driver camera is supplemented by a camera capturing the driver cabin. Together with steering wheel and vehicle motion information, DD-Pose paves the way for holistic driver analysis. Our experiments show that the new dataset offers a broad distribution of head poses, comprising an order of magnitude more samples of rare poses than a comparable dataset. By an analysis of a state-of-the-art head pose estimation method, we demonstrate the challenges offered by the benchmark. The dataset and evaluation code are made freely available to academic and non-profit institutions for non-commercial benchmarking purposes
Molecular detection of Treponema species organisms in foremilk and udder cleft skin of dairy cows with digital dermatitis
No full textIdentification of reservoirs and transmission routes of digital dermatitis (DD)-associated Treponema spp. is considered an effective means for controlling DD infection in dairy cows. The objective of this study is to identify and characterize the potential reservoir niches for DD-associated Treponema spp. from healthy udder cleft skin and foremilk in lactating dairy cows. A large dairy farm was visited weekly from March to July 2015. Clinical investigation revealed that a total of 25 lame cows had DD lesions located at the plantar aspect of the interdigital cleft. A total of 75 samples, three per cow, were collected including deep swabs from DD lesions (n = 25), non-aseptically collected foremilk samples (n = 25) and skin swabs from udder cleft (n = 25). Treponema spp. were identified using nested PCR assays and confirmed by DNA sequencing. Results revealed that Treponema phagedenis (T. phagedenis)-like was the most identified species in the foremilk 40% (10/25), in comparison with DD lesions and udder cleft skin samples with 32% (8/25) and 20% (5/25), respectively. On the other hand, Treponema pedis (T. pedis) was the most identified species in the udder cleft skin 80% (20/25), in comparison with DD lesions and foremilk samples with 68% (17/25) and 60% (15/25), respectively. None of the examined samples were identified by PCR as containing DNA from Treponema medium (T. medium) or Treponema vincentii (T. vincentii)-like. To the best of our knowledge, this is the first report for detection of T. phagedenis-like and T. pedis from healthy skin of udder cleft and foremilk samples. Detection of DD Treponema spp. from udder cleft skin and foremilk samples indicates that these sites could be potential reservoirs for spirochetes involved in DD. Udder cleft skin and foremilk may have a role in transmission routes of DD Treponema in dairy farms.info:eu-repo/semantics/acceptedVersio
Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species
No full textAbstract Digital dermatitis (DD) presents as painful, ulcerative or proliferative lesions that lead to bovine lameness affecting economic efficiency and animal welfare. Although DD etiological agent(s) have not been established, it is widely accepted that DD is a polymicrobial disease significantly associated with species of Treponema and the non-linear disease progression may be attributed to interactions among infecting bacteria. We postulated the morphological changes associated with DD lesion grades are related to interactions among infecting species of Treponema. We developed a novel species-specific qPCR that can identify the absolute abundance of the four of the most common species of Treponema in DD, T. phagedenis, T. medium, T. pedis and T. denticola, in a single reaction. We found species abundance and the number of distinct Treponema species present is higher in active, ulcerative lesions than in healing lesions, chronic lesions, and DD-free skin. Treponema spp. were present in both DD-free skin and M3 lesions following treatment with oxytetracycline. We have also found positive correlations among T. phagedenis, T. medium and T. pedis indicating they are significantly more likely to be found together than apart and their absolute quantities tend to increase together, a relationship which is not present with T. denticola. Further, we found Treponema, particularly viable T. denticola, in lesions 5 days post treatment with oxytetracycline (M3). Our findings suggest that pathogenicity may be closely associated with Treponema abundance, particularly T. phagedenis, T. medium and T. pedis, and interactions among them, independent of T. denticola. Our results provide a novel, consistent method to identify species of Treponema within DD lesions and associate Treponema spp. and abundance with morphological changes related to host pathogenicity
Developmental Disorders (DD) and Fibromyalgia (FM), we might be able to develop both treatments at once!?
No full textThis was written when the author was 2nd year undergraduate student in Kyushu University. In this paper, I explain following 3 topics: ①Development Disorders (DD), are one of physical disabilities ②study of pain (brain, neuron, and fibromyalgia (FM)) ③ DD and FM, we might be able to develop both treatments at once!
Developmental Disorders (DD) and Fibromyalgia (FM), we might be able to develop both treatments at once!?
No full textThis was written when the author was 2nd year undergraduate student in Kyushu University. In this paper, I explain following 3 topics: ①Development Disorders (DD), are one of physical disabilities ②study of pain (brain, neuron, and fibromyalgia (FM)) ③ DD and FM, we might be able to develop both treatments at once!
Developmental Disorders (DD) and Fibromyalgia (FM), we might be able to develop both treatments at once!?
No full textThis was written when the author was 2nd year undergraduate student in Kyushu University. In this paper, I explain following 3 topics: ①Development Disorders (DD), are one of physical disabilities ②study of pain (brain, neuron, and fibromyalgia (FM)) ③ DD and FM, we might be able to develop both treatments at once!
Evidence for B-0 -> D+D- and observation of B-->(DD-)-D-0 and B-->(DD*-)-D-0 decays
No full textWe report evidence for B-0 -> D+D- and the first observation of the decay modes B-->(DD-)-D-0 and B-->(DD*-)-D-0 based on a sample of 152x10(6) B (B) over bar events collected by the Belle detector at KEKB. The branching fractions for B-0 -> D+D-, B-->(DD-)-D-0, and B-->(DD*-)-D-0 are found to be (1.91 +/- 0.51 +/- 0.30)x10(-4), (4.83 +/- 0.78 +/- 0.58)x10(-4), and (4.57 +/- 0.71 +/- 0.56)x10(-4), respectively. Charge asymmetries in the B-->(DD-)-D-0 and B-->(DD*-)-D-0 channels are consistent with zero
The Influence of Intellectual/Developmental Disabilities (I/DD) Curriculum Content on Career Interests for Social Work Students
No full textThis study examines how curriculum content on Intellectual/Developmental Disabilities (I/DD) impacts the career interests of social work students. With the growing demand for social workers proficient in I/DD services, it is crucial to understand how educational experiences shape career choices. Using a QUANT→Qual mixed-method approach, this quasi-experimental pre/post-test survey combined quantitative data (N 66 [experimental group]; N =103 [control group] and open-ended survey questions (N = 66 [experimental group]; N =103 [control group] from social work students.
The author-constructed survey assessed student attitudes, perceptions, preparedness, ability to identify I/DD, knowledge of I/DD, and willingness to practice with individuals with I/DD before and after exposure to I/DD-focused curriculum content over a 15-week period. The quantitative findings indicated that exposure to I/DD-focused curriculum content significantly improved students’ preparedness, ability to identify I/DD, knowledge about I/DD, and willingness to work with this population. Qualitative findings indicated that students who completed the I/DD courses reported increased confidence and interest in pursuing careers involving I/DD roles. The study findings emphasize the importance of including dedicated I/DD content in social work education to better equip future practitioners
Multilocus sequence typing of pathogenic treponemes isolated from cloven-hoofed animals and comparison to treponemes isolated from humans
No full textTreponema species are implicated in many diseases of humans and animals. Digital dermatitis (DD) treponemes are reported to cause severe lesions in cattle, sheep, pigs, goats, and wild elk, causing substantial global animal welfare issues and economic losses. The fastidiousness of these spirochetes has previously precluded studies investigating within-phylogroup genetic diversity. An archive of treponemes that we isolated enabled multilocus sequence typing to quantify the diversity and population structure of DD treponemes. Isolates (n121) were obtained from different animal hosts in nine countries on three continents. The analyses herein of currently isolated DD treponemes at seven housekeeping gene loci confirm the classification of the three previously designated phylogroups: the Treponema medium, Treponema phagedenis, and Treponema pedis phylogroups. Sequence analysis of seven DD treponeme housekeeping genes revealed a generally low level of diversity among the strains within each phylogroup, removing the need for the previously used "-like" suffix. Surprisingly, all isolates within each phylogroup clustered together, regardless of host or geographic origin, suggesting that the same sequence types (STs) can infect different animals. Some STs were derived from multiple animals from the same farm, highlighting probable within-farm transmissions. Several STs infected multiple hosts from similar geographic regions, identifying probable frequent between-host transmissions. Interestingly, T. pedis appears to be evolving more quickly than the T. medium or T. phagedenis DD treponeme phylogroup, by forming two unique ST complexes. The lack of phylogenetic discrimination between treponemes isolated from different hosts or geographic regions substantially contrasts with the data for other clinically relevant spirochetes
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