1,720,966 research outputs found
DESIGN, VALIDATION AND IMPLEMENTATION OF FAST MOLECULAR DIAGNOSTIC TESTS IN EMERGENCY MEDICINE
No full textCilj istraživanja: glavni cilj istraživanja je uspostaviti brzu molekularnu dijagnostiku na
mjestu skrbi za pacijenta, tzv. POCT (od eng. „point-of-care tests“) molekularnu dijagnostiku
u odjelu hitne medicine (OHBP od Objedinjeni hitni bolnički prijam) Kliničkog bolničkog
centra Rijeka.
Ispitanici i metode: u prvi i drugi dio istraživanja u kojemu smo usporedili direktni qPCR
(dqPCR) sa standardnim qPCR (stndqPCR), optimizirali i validirali dqPCR u POCT, uključili
smo 126 ispitanika podijeljenih u 4 grupe: pacijenti, zaposlenici KBC-a Rijeka, pacijenti
hospitalizirani u COVID-19 respiracijskom centru i pacijenti s potvrđenom COVID-19
infekcijom dva ili više tjedana prije testiranja. Treći dio istraživanja u kojem smo tijekom 4
mjeseca analizirali implementiranu POCT – PCR metodu proveli smo na preko 10.000
pacijenata i zaposlenika KBC-a Rijeka čiji su se uzorci slali u naš Laboratorij na dijagnostičku
molekularnu analizu. Za istraživanje izabrali smo Seegene-ov Allplex SARS-CoV-2 paket
kemikalija koji dolazi u kompletu s računalnim programom „SARS-CoV-2 Viewer“ za
automatiziranu interpretaciju rezultata, a rezultate smo analizirali primjenom Seegene i strožih
naših kriterija.
Rezultati: analizirajući i uspoređujući osjetljivost i specifičnost dqPCR i POCT varijante
dqPCR sa stndqPCR-a broj pozitivnih, negativnih ili neodređenih rezultata bilo je u snažnom
slaganju s rezultatima dobivenih stndqPCR-om. Nadalje, korelacija sa stndqPCR-a bila je još
izraženija kada su za stratifikaciju dobivenih rezultata korišteni naši kriteriji te smo na temelju
učinjenih analiza i dobivenih rezultata zaključili da je POC-dqPCR, modificirani postupak
dqPCR-a u kojem medicinsko osoblje bez prethodnog iskustva u molekularnoj dijagnostici
može pouzdano koristiti prethodno pripremljenu reakcijsku smjesu za detekciju SARS-CoV-2
u odjelima hitne medicine i/ili sličnim odjelima koji neposredno skrbe za oboljele. Dodatno
smo validirali metodu korištenjem standardiziranih uzoraka Svjetske zdravstvene organizacije
te na zbirnim uzorcima djelatnika OHBP-a KBC Rijeka.
Zaključak: Seegene-ov Allplex SARS-CoV-2 test smo prilagodili u direktni qPCR test sličan
POCT-u koji može učinkovito i pouzdano provoditi medicinsko osoblje u Laboratoriju za brzu
molekularnu dijagnostiku u hitnoj medicini, OHBP-a KBC Rijeka.Objectives: main objective of this research is to establish fast, point – of – care molecular
diagnostics, at the Emergency department of the Clinical hospital center Rijeka.
Patients and methods: in the first and second part of research we compared direct qPCR with
standard qPCR, optimized and validated dqPCR in the POC setting. We included 126 subjects
in 4 groups: patients from ED, hospital employees, patients hospitalized in COVID-19
respiratory center and patients in whom COVID -19 infection has been confirmed two or more
weeks before testing. For the third par we included more than 10.000 patients and employee
samples which were analyzed in out Laboratory for the diagnostic molecular analysis. Seegene
Allplex SARS-CoV-2 reagents were used coupled with SEEGENE SARS-CoV-2 Viewer
software for the automated data interpretation. For data interpretation we applied both Seegene
and our – more stringent criteria.
Results: comparing sensitivity and specificity of dqPCR and POCT dqPCR with stndqPCR we
obtained strong correlation of results in terms of positive, negative, and undetermined results.
Furthermore, correlation was even more pronounced applying our criteria. Based on the results
obtained we conclude that POC-dqPCR, modified dqPCR in which medical staff without
previous molecular diagnostics can reliably use SARS-COV-2 detection reagents prepared in
advance in emergency departments and/or other departments which provide direct patient care.
We, additionally, validated the method using standardized World health organization samples
and pooled samples from the Clinical hospital center Rijeka Emergency department staff.
Conclusion: We adapted a Seegene Allplex SARS-CoV-2 test into a direct, point-of-care like,
test which can be efficiently and reliably used in Laboratory for fast molecular diagnostics in
emergency medicine of the Clinical hospital Center Rijeka
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Znanost temeljena na dokazima u nefrologiji, dijalizi i transplantaciji bubrega
Full text linkNephrology is a young branch of internal medicine whose development began in the 60s of the last century by introducing renal replacement therapy in everyday clinical practice. After nearly half a century of publicity, it has been shown that a large number of studies are of questionable quality and it has been concluded that the nephrology lacked randomized controlled trials of high quality. A series of actions were carried out in co-operation with the renal Cochrane group with the aim of advancing the profession and giving clear recommendations for future clinical research. Our group in Rijeka has three review titles accepted in the renal Cochrane group and we have been drafting a protocol on the prevention of contrast nephropathy.Nefrologija je mlada grana interne medicine čiji razvoj započinje 60-tih godina prošlog stoljeća uvođenjem nadomještanje bubrežne funkcije u svakodnevnu kliničku praksu. Nakon gotovo pola stoljeća i publiciranog velikog broja studija pokazalo se je da je veliki broj njih upitne kvalitete te je zaključeno da nefrološkoj struci nedostaju randomizirane studije visoke kvalitete. Učinjeno je niz akcija u suradnji i s nefrološkom Cochrane grupom s ciljem unapređenja struke te su date jasne preporuke za buduća klinička istraživanja. Naša grupa u Rijeci ima od strane nefrološke Cochrane grupe prihvaćena tri naslova i u fazi smo izrade protokola o prevenciji kontrastne nefropatije
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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