102,742 research outputs found
Kinetic and spectroscopic characterization of the putative monooxygenase domain of human MICAL-1
No full textMICALs form a conserved multidomain protein family essential for cytoskeletal rearrangements. To complement structural information available, we produced the FAD-containing monooxygenase-like domain of human MICAL-1 (MICAL-MO) in forms differing for the presence and location of a His-tag, which only influences the protein yields. The K(m) for NADPH of the NADPH oxidase reaction is sensitive to ionic strength and type of ions. The apparent k(cat) (pH 7) is limited by enzyme reduction by NADPH, which occurs without detectable intermediates, as established by anaerobic rapid reaction experiments. The sensitivity to ionic strength and type of ions and the pH dependence of the steady-state kinetic parameters extend MICAL-MO similarity with enzymes of the p-hydroxybenzoate hydroxylase class at the functional level. The reaction is also sensitive to solvent viscosity, providing a tool to monitor the conformational changes predicted to occur during turnover. Finally, it was confirmed that MICAL-MO promotes actin depolymerization, and it was shown that F-actin, but not G-actin, stimulates NADPH oxidation by increasing k(cat) and k(cat)/K(NADPH) (approximate to 15 and approximate to.200-fold, respectively) with an apparent K(m) for actin of 4.7 mu M, under conditions that stabilize F-actin. The time-course of NADPH oxidation shows substrate recycling, indicating the possible reversibility of MICAL effect
Bibliographie Hilarion G. Petzold 1958 – 2009 mit Anhang als Einführung
No full textDieses Archiv enthält die Gesamtbibliographie der Werke des Autors nebst einiger Texte „Über H. G. Petzold“ im Schlussteil der Bibliographie sowie einen Anhang mit einer Einführung in die Architektur des Werkes in seinem wissenslogischen Aufbau als Ausarbeitung seines „Tree of Science Modells“ (2007).This archive contains the complete bibliography of the author and some texts about H. G. Petzold, moreover an epilogue with an introduction to the architecture of the works in its epistemological structure and composition and as an elaborations of Petzold’s „Tree of Science Modell (2007).https://www.fpi-publikation.de/polyloge/01-2009-petzold-h-g-gesamtbibliographie-h-g-petzold-1958-2009-updating-november2009/peerReviewedpublishedVersio
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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3346: Samuel G. Freedman, author, 2013
No full textPhotograph of author Samuel G. Freedman, at NT Daily Slash meeting in the Mayborn School of Journalism at UNT
Kinetic and spectroscopic characterization of the putative monooxygenase domain of human MICAL-1
No full textTunnel induced settlement damage: A case study to improve damage prediction for facades
No full textThe tunnel boring process introduces soil settlements. Damage to nearby building could occur if settlements become too large. A reliable damage prediction model is necessary too asses the risks of damage to tunnel induced settlements. A widely used method for damage prediction is the Limiting Tensile Strain Method (LTSM). The LTSM models a masonry building as a weightless, isotropic, linear-elastic, rectangular beam on 2 supports. Although the LTSM is an easy method to use, it has its limitations. For facades for instance the perforation of the wall, which introduces weak spots in the wall and reduces the stiffness, is neglected. In this project the applicability of the LTSM in the case of facades is studied to improve damage predictions in the case of facades. A case study is performed to examine the response of facades in the Daniel Stalpertstraat due to settlements caused by the tunnelling process of the North/Southline. This field data is used to find a calibrated 2D numerical model of the facades. The calibrated numerical model is then subjected to larger settlements to obtain the behaviour of the facades at large settlements. Using linear and nonlinear analyses of the numerical model it is evaluated how accurate and how conservative 4 damage prediction models are. The first two LTSM models were examined: the standard LTSM model with E/G=2.6 and one with E/G=12.5. Based on the findings in the linear analyses also two models based on conventional beam theory were examined for their applicability: portal frame model and Forget-Me-Not model. With linear analyses it is checked how reliable the methods are in terms of strains and deformation under the imposed settlements. With nonlinear analyses the conservativeness of each method in terms of damage is evaluated by comparing the crack with found I the numerical model to the crack width calculated with the damage prediction models. The LTSM with E/G=12.5 gives the best results according to linear numerical analyses results. The LTSM with E/G provided the same curvature and shear distortion as found din the numerical analyses, the strains were approximated with 90%. The Forget-Me-Not model shows the best results according to the nonlinear analyses results. At large settlements this model provides the same results as found in the nonlinear numerical analysis results.Structural MechanicsStructural EngineeringCivil Engineering and Geoscience
The Right to Strike under the United States Constitution: Theory, Practice, and Possible Implications for Canada
No full textAnswering critics of the Canadian Supreme Court's judgment in B.C. Health, the author argues that the Court laid the foundation for a principled and durable doctrine protecting constitutional labour rights, one that goes directly to the heart of the matter — the inequality of workers’ power in the employment relation. In the author’s view, two paths could lead from B.C. Health to the recognition of Charter protec- tion for a right to strike: one that treats the right as an accessory to col- lective bargaining, and one that upholds the right directly on the basis of the Charter values of equality and participation. The author supports the latter approach, contending that constitutional rights should be defined in relation to fundamental values, in a way that is not contingent on time-bound or fact-sensitive assessments about the role of strikes within a particular collective bargaining regime. Although a Charter right to strike may involve the courts in difficult choices about when to defer to legislative policy decisions, and courts may lack the institutional capac- ity to deal effectively with labour law issues, the author points out that judges can look to ILO standards for expert guidance. Noting that the U.S. experience in this area might be of considerable use to Canadians, the author concludes by providing an overview of American case law concerning a constitutional right to strike.Peer reviewe
G-Rank: Unsupervised Continuous Learn-to-Rank for Edge Devices in a P2P Network
No full textRanking algorithms in traditional search engines are powered by enormous training data sets that are meticulously engineered and curated by a centralized entity. Decentralized peer-to-peer (p2p) networks such as torrenting applications and Web3 protocols deliberately eschew centralized databases and computational architectures when designing services and features. As such, robust search-and-rank algorithms designed for such domains must be engineered specifically for decentralized networks, and must be lightweight enough to operate on consumer-grade personal devices such as a smartphone or laptop computer. We introduce G-Rank, an unsupervised ranking algorithm designed exclusively for decentralized networks. We demonstrate that accurate, relevant ranking results can be achieved in fully decentralized networks without any centralized data aggregation, feature engineering, or model training. Furthermore, we show that such results are obtainable with minimal data preprocessing and computational overhead, and can still return highly relevant results even when a user’s device is disconnected from the network. G-Rank is highly modular in design, is not limited to categorical data, and can be implemented in a variety of domains with minimal modification. The results herein show that unsupervised ranking models designed for decentralized p2p networks are not only viable, but worthy of further research.https://github.com/awrgold/G-RankComputer Scienc
Author inscription in The Chinese slave-girl: a story of woman's life in China
No full textThis edition includes a gift inscription by author Rev. J.A. Davis, "To Rev. A. G. Russell with the warmest regards of the author J.A. Davis."Davis, John Agnell, 1839-1897
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