21 research outputs found
Sliced Island Tragal Cartilage Perichondrial Composite Graft in Type I Tympanoplasty: Early Results and Experience
No full textAbstract: Aim: The purpose of this study is to evaluate anatomical and audiological results using technique of sliced island of tragal cartilage perichondrial composite graft in type I tympanoplasty. Study Design: Prospective study. Methods and Materials: A total of 56 type I tympanoplasties (51 primary and 5 revision ears) were operated using sliced island tragal cartilage graft from December 2011 to August 2012 in M.I.M.E.R Medical College and Sushrut ENT Hospital with a follow up ranging from 6 months to 16 months. Results: Our early results revealed a successful closure of the tympanic membrane in all patients at the end of the follow up period ranging from 6 to 16 months. The average postoperative Air Bone Gap was 10.035+/-1.7473 dB. Conclusion: To our knowledge, this is the first study which involves the modification of the sliced tragal cartilage into island as a sliced tragal cartilage island perichondrial composite graft in tympanoplasty. This technique is reliable not only in terms of successful closure of tympanic membrane perforations but also overall reduction of residual and recurrent perforations
Lopsided Blood-thinning Drug Increases the Risk of Internal Flow Choking and Shock Wave Generation Causing Asymptomatic Stroke
No full textLopsided Blood-thinning Drug Increases the Risk of Internal Flow Choking and Shock Wave Generation Causing Asymptomatic Stroke
Author Block: V R SANAL KUMAR, ISRO; S.K.Choudhary, AIIMS; P.K.Radhakrishnan, GU; Suresh Menon, GT; Vrishank Raghav, AU; K.K.N Namboodiri, Sapna E.Sreedharan, SCTIMST; Bharath R.S, Nichith C, C.Oommen, IISc; V.Sankar, IITK; A.Sukumaran, KCT; Arun K, DHMMC; A.Pal, Tharikaa R.K, AU, Abhirami R, AIMS.
Introduction
Consequence of lopsided blood-thinning-drug, lowering blood-viscosity (BV), is bleeding and very frequently asymptomatic-hemorrhage (AH) and the acute-heart-failure (AHF) happen. V.R.S.Kumar et al. (2020) reported that such asymptomatic episodes are due to the internal flow choking in the cardiovascular system (CVS) at a critical blood-pressure-ratio (BPR), which is regulated by biofluid/blood heat capacity ratio (BHCR).
Methods
The closed-form-analytical-methodology is used for correlating BV, BPR, BHCR, vessel geometry and ejection fraction (EF). In vitro method is used for the BHCR estimation of healthy subjects. In silico method is used for demonstrating the Sanal flow choking.
Results
The analytical models reveal that the relatively high and low BV are risk factors of internal flow choking. In vitro study shows that N2, O2, CO2 & Ar gases are predominant in fresh-blood samples of the healthy subjects at a temperature range of 37-400 C (98.6-1040 F), which increases the risk of flow-choking. In silico results demonstrated the Sanal flow choking followed by the shock wave generation and pressure-overshoot in a simulated artery with the divergent/bifurcation region.
Conclusions
An overdose of blood-thinning drug reduces BV and increases Reynolds number causing high-turbulence leading to the Sanal flow choking. Asymptomatic stroke could be diminished by concurrently lessening the BV and flow turbulence by rising thermal tolerance level in terms of BHCR or by decreasing the BPR. In conclusion, BPR must always be lower than 1.8257 as dictated by the lowest BHCR of the evolved gas for prohibiting asymptomatic stroke
Reinforcement of Sliced Tragal Cartilage Perichondrium Composite Graft with Temporalis Fascia in Type I Tympanoplasty: Our Techniques and Results
No full textAbstract: In our otological practice, we prefer sliced tragal cartilage- perichondrium grafting for reconstruction of the tympanic membrane perforations. In an attempt to reduce residual and recurrent perforations in our series of cartilage tympanoplasty using sliced tragal cartilage perichondrium composite graft, we reinforce tragal cartilage with temporalis fascia in large perforations. Aim: The purpose of this study is to evaluate anatomical and audiological results after primary cartilage tympanoplasty using sliced tragal cartilage reinforced with temporalis fascia in large perforations.Methods and Materials: We carried a prospective study of 28 patients operated from March 2010 to January 2011 in M.I.M.E.R Medical College and Sushrut ENT Hospital using sliced tragal cartilage reinforced with temporalis fascia. The surgical technique is described in detail.Results: Successful closure of the tympanic membrane was achieved in all patients at 2 years of follow up. The average postoperative Air Bone Gap was 9.6429+/-2.6557 dB. Conclusion: Reinforcement of temporalis fascia with sliced tragal cartilage is a reliable technique for tympanoplasty, especially in large perforations. To our knowledge, this is the first study involving the reinforcement of sliced tragal cartilage with temporalis fascia in type I tympanoplasty
Sliced Island Tragal Cartilage Perichondrial Composite Graft in Type I Tympanoplasty: Early Results and Experience
No full textAim: The purpose of this study is to evaluate anatomical and audiological results using technique of sliced island of tragal cartilage perichondrial composite graft in type I tympanoplasty.Study Design: Prospective study.Methods and Materials: A total of 56 type I tympanoplasties (51 primary and 5 revision ears) were operated using sliced island tragal cartilage graft from December 2011 to August 2012 in M.I.M.E.R Medical College and Sushrut ENT Hospital with a follow up ranging from 6 months to 16 months. Results: Our early results revealed a successful closure of the tympanic membrane in all patients at the end of the follow up period ranging from 6 to 16 months. The average postoperative Air Bone Gap was 10.035+/-1.7473 dB.Conclusion: To our knowledge, this is the first study which involves the modification of the sliced tragal cartilage into island as a sliced tragal cartilage island perichondrial composite graft in tympanoplasty. This technique is reliable not only in terms of successful closure of tympanic membrane perforations but also overall reduction of residual and recurrent perforations
Tree bark scrape fungus: a potential source of laccase for application in bioremediation of non-textile dyes
Get PDFAlthough laccase has been recognized as a wonder molecule and green enzyme, the use of low yielding fungal strains, poor production, purification, and low enzyme kinetics have hampered its large-scale application. Thus,this study aims to select high yielding fungal strains and optimize the production, purification, and kinetics of laccase of Aspergillus sp. HB-RZ4. The results obtained indicated that Aspergillus sp. HB-RZ4 produced a significantly large amount of laccase under meso-acidophilic shaking conditions in a medium containing glucose and yeast extract. A 25 μM CuSO4 was observed to enhance the enzyme yield. The enzyme was best purified on a Sephadex G-100 column. The purified enzyme resembled laccase of A. flavus. The kinetics of the purified enzyme revealed high substrate specificity and good velocity of reaction,using ABTS as a substrate. The enzyme was observed to be stable over various pH values and temperatures. The peptide structure of the purified enzyme was found to resemble laccase of A. kawachii IFO 4308. The fungus was observed to decolorize various dyes independent of the requirement of a laccase mediator system. Aspergillus sp. HB-RZ4 was observed to be a potent natural producer of laccase, and it decolorized the dyes even in the absence of a laccase mediator system. Thus, it can be used for bioremediation of effluent that contains non-textile dyes. © 2020 Sayyed et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
