1,721,010 research outputs found
HIV-1 p17 binds heparan sulfate proteoglycans to activated CD4(+) T cells
No full textWe have previously shown that HIV-1 p17 binds to activated peripheral blood mononuclear cells and enhances secretion of pro-inflammatory
cytokines, but we were unable to define a ligand on activated cells. In this work we evaluate the hypothesis that HIV-1 p17 may be a heparin/heparan
sulfate-binding protein.
HIV-1 p17 contains C- and N-terminal sequences with positively charged residues and a consensus cluster for heparin binding.We demonstrated
by affinity chromatography that HIV-1 p17 binds strongly to heparin-agarose at physiological pH. Soluble heparins and heparan sulfate but not
chondroitin 4-sulfate and dextran sulfate inhibit binding of HIV-1 p17 to heparin solid phase and to activated CD4+ T cells. Furthermore the
inhibition of cell sulfatation by chlorate treatment completely counteracts HIV-1 p17 binding to activated cells.
These results indicate for the first time that HIV-1 p17 can be ascribed to the heparin binding protein family and suggest that this interaction
might play a key role in the ability of the protein to induce an inflammatory effect on activated cells
HIV p17 reverses the anti-inflammatory activity of IL-4 on IL-15 stimulated monocytes and modulates their ability to secrete MIP-1 alpha
No full textMonocytes play a central role in the immune system by producing and reacting to different soluble factors. Cytokine dysregulation is an hallmark
in HIV-infected individuals and it is one of the most significant factors leading to impaired immunity in HIV/AIDS disease.
This study investigates the possibility of modulation in the secretion of some inflammatory cytokines and chemokines induced by HIV p17 in
monocytes.
The results show that p17, while ineffective on resting monocytes, exerts an inflammatory action on IL-4 mediated inhibition of TNF-alpha and
IFN- gamma production induced by IL-15 stimulation. In addition, p17 is able to reduce MIP-1alpha secretion, but unable to influence IL-6 production.
The ability of HIV p17 to contribute to an altered pattern of secreted soluble factors might imply a key role for this viral protein in the development
of AIDS pathogenesi
Impiego di una formula adattata a base di aminoacidi in bambini con allergia al latte vaccino
No full textHIV-1 p17 matrix protein interacts with heparan sulfate side chain of CD44v3, syndecan-2, and syndecan-4 proteoglycans expressed on human activated CD4+ T cells affecting tumor necrosis factor alpha and interleukin 2 production.
No full textAllergia alle proteine del latte vaccino: tolleranza di una formula adattata a base di aminoacidi.
No full textInhibitory effects of galanin on growth hormone (GH) release in cultured GH-secreting adenoma cells: comparative study with octreotide, GH-releasing hormone, and thyrotropin-releasing hormone.
No full text
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Monoclonal antibodies for the serodiagnosis of Campylobacter pylori-associated gastritis
No full textVariations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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