1,721,030 research outputs found
First report of grapvine pinot gris virus in Emilia-Romagna and Veneto regions
No full textA recently discovered trichovirus, grapevine pinot gris virus (GPGV), was suggested to be associated with a new grapevine disease in Trentino. the main symptoms are represented by leaf deformation with chlorotic mottling and various mosaic pattern. Shoots are also malformed, showing short internodes and abnormal branching. Diseased vines show a stunting, low vigour, reduced yields and low quality of the berries. Similar symptoms have been observed in some viticultural areas of Emilia-Romagna and Veneto. RT-PCR analysis were carried out to check the virological status of both symptomatic and symptomless grapevines. Results have revealed the presence of GPGV in all symptomatic vines but also in some apparently symptomless samples. PCR products were sequenced and all sequences showed a high identity with the published sequence of GPGV. Further investigations are in progress to better define the distribution and aetiology of this grapevine disease in the Emilia-Romagna and Veneto vineyards
Valutazione di nuove varietà di albicocco nei confronti del giallume europeo delle drupacee (ESFY) mediante l'utilizzo di tecniche molecolari.
No full textLa valutazione della sensibilità di numerose varietà di albicocco nei confronti di ESFY, associato alla presenza di candidatus Phytoplasma prunorum, realizzata nell'ambito di un progetto triennale (2006-2009), finanziato dal CRPV e dalle principali O.P. ha dimostrato come la quasi totalità delle cv. diffuse negli ultimi anni in Emilia-Romagna, presenti un preoccupante livello di suscettibilità a tale malattia. nel corso del biennio 2011-2012, nell'ambito di un progetto regionale, è stata così effettuata una prima valutazione del comportamento, nei confronti di ESFY, di nuove varietà di albicocco (Faralia, Farbaly, Petra), innestate su mirabolano
Utilizzo del virus del rachitismo cespuglioso del pomodoro (TBSV) come vettore virale per l’induzione di resistenza al virus della vaiolatura delle drupacee (PPV)
No full textDetection and molecular characterization of viruses infecting Actinidia spp.
No full textKiwifruit (genus Actinidia) is an important horticultural crop grown in the temperate regions. The four world’s largest producers are China, Italy, New Zealand and Chile. More than 50 species are recognized in the genus but the principal species in cultivation are A. deliciosa and A. chinensis. In Italy, as well as in many other countries, the kiwifruit crop has been considered to be relatively disease free and then no certification system for this species has been developed to regulate importation of propagation plant material in the European Union. During the last years a number of fungal and bacterial diseases have been recorded such as Botrytis cinerea and Pseudomonas syringae pv. actinidiae. Since 2003, several viruses and virus-like diseases have been identified and more recent studies demonstrated that Actinidia spp can be infected by a wide range of viral agents. In collaboration with the University of Auckland we have been detected thirteen different viral species on kiwifruit plants. During the three years of my PhD I worked on the characterization of Cucumber mosaic virus (CMV) and Pelargonium zonate spot virus (PZSV). The determination of causal agents has been based on host range, symptom expression in the test plant species and morphological properties of the virus particles using transmission electron microscopy (TEM) and using specific oligonucleotide primers in reverse transcription-polymerase chain reaction (RT-PCR). Both viruses induced several symptoms on kiwifruit plants. Moreover with new technologies such as high-throughput sequencing we detected additional viruses, a new member of the family Closteroviridae and a new member of the family Totiviridae. Taking together all results of my studies it is clear that, in order to minimize the risk of serious viral disease in kiwifruit, it is vital to use virus-free propagation material in order to prevent the spread of these viruses
Studies on a new grapevine disease in Trentino vineyards.
No full textSymptoms of stunting, chlorotic mottling and leaf deformation with reduced yields and low quality of berries, were observed on cv. Pinot gris, in Trentino vineyards since 2003. Afterwards, similar symptoms were reported from cvs. Traminer, Pinot Noir in Trentino and Friuli-Venezia Giulia. Analysis by high throughput sequencing led to the discovery of a new trichovirus, named grapevine pinot gris virus (GPGV), closely related to grapevine inner berry necrosis (GINV). To define the aetiology and the biological features of the disease, field, greenhouse and laboratory studies were made. Field observations on a time span of three years (2010-12) indicated a progressive increase of diseased vines from 14,7 to 33,9 % in a cv. of Pinot gris vineyard whereas the incidence in a cv. Traminer vineyard was stationary and lower (ca.3%). RT-PCR assays showed that, in these vineyards, GPGV was present in all symptomatic but also in 70% of symptomless vines. Biological indexing demonstrated that GPGV is efficiently transmitted by grafting to Vitis riparia and V. vinifera cvs Pinot gris and Traminer inducing specific symptoms. A screening on different varieties and vineyards in the area suggests a widespread distribution of GPGV. Its presence appears closely associated with a disease condition although latent infections in symptomless vines can also occur
Il giallume europeo delle drupacee.
No full textIn Italia ed in Europa, particolarmente nei paesi che si affacciano sul Mediterraneo, sono presenti su varie drupacee coltivate e spontanee diverse sindromi causate dal fitoplasma del giallume europeo delle drupacee; alcune di queste come l’accartocciamento fogliare clorotico dell’albicocco e la leptonecrosi del susino sono note da tempo ed ampiamente diffuse, mentre su altre specie, come il pesco, la gravità appare minore, anche negli ultimi anni si è assistito ad un preoccupante aumento della malattia negli impianti peschicoli del Italia Settentrionale. Altre specie come il susino europeo sono invece tolleranti, anche talvolta alcune cultivar possono evidenziare sintomi lievi. Il patogeno viene trasmesso dalla psilla delle drupacee, Cacopsylla pruni (Scopoli).
Si prendono in considerazione le principali sindromi causate dal fitoplasma del giallume europeo delle drupacee nel nostro Paese, indicando anche il comportamento di varietà e portinnesti nei confronti della malattia. Viene inoltre dedicata una particolare attenzione alle modalità di trasmissione, ai metodi diagnostici e alle possibili forme di contenimento della malattia: molto importante risulta la scelta del materiale vivaistico per la costituzione di impianti sani, mentre permangono ancora dubbi circa la capacità di limitare la diffusione della malattia con la difesa insetticida in presenza di popolazioni ridotte del vettore
Aspetti epidemiologici e valutazione della resistenza alla sharka in nuovi genotipi di pesco.
No full textSono indicati i principali aspetti epidemiologici del plum pox virus: i vari isolati virali e le loro principali caratteristiche, le modalità di trasmissione a breve, media e lunga distanza, la diffusione secondaria dei vari isolati negli impianti di albicocco, pesco e susino, il ruolo di drupacee spontanee ed erbacee bienni e perenni nel ciclo biologico del patogeno. Vengono infine indicate le principali modalità di controllo della maalttia, basate essenzialmente sulla eradicazione delle piante infette e sul reperimento di germoplasma resistente al patogeno
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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