1,721,084 research outputs found
Salmonella spp. in Sardinian slaughter pigs: prevalence, serotypes and genotypic characterization
Full text linkIn order to further the knowledge about Salmonella prevalence at farm level and pork meat in
Sardinia, the prevalence and the sources of Salmonella at 5 pig slaughterhouses (slaughtered pigs
and environment) and 2 swine farms (feed and faeces), were investigated and the isolates
characterized. Salmonella was isolated from mesenteric lymph nodes (30.5%), colon content
(16.4%), carcass and liver (both 14.1%); as regards to the slaughterhouse environment Salmonella
prevalence was 37.5% in the any contact surfaces samples and 35.2% in contact surfaces samples.
Salmonella could not be isolated in any of the samples taken at farm. N. 31 pigs were carriers of
Salmonella in lymph nodes and/or colon content, but among this only 8 were positive at carcasses
level. Eight different serotypes were detected; the most common were S. derby (43%) and S.
typhimurium (23%). The prevalent S. typhimurium phage type was DT193 (50%). N.32 isolates
were found to be resistant to more than one antimicrobial (MDR). Heterogeneous integronassociated
resistance genes were present in the isolates. PFGE permitted the resolution of XbaI
macrorestriction fragments of the Salmonella strains into 20 distinct pulsetypes. The combinated
application of PPA and PFGE gave additional useful information to trace the Salmonella routes in
abattoirs. To reduce Salmonella prevalence some preventive measures should be encouraged: the
identification of origin of infected slaughtered animals should be performed, and the direct and
cross-contamination of carcasses avoided according to HACCP principles in association with good
hygiene procedures (GHP)
Techniques of immunofluorescence and confocal microscopy applied to the study of syngnathids gonadal structure and development and to the dopaminergic control of the reproduction in teleosts
Full text linkReproduction is an indispensable function for the perpetuation of the species and is perfomed with extraordinarily diverse reproductive strategies (adaptations that improve the chances of fertilization and/or increase the survaival rate of offsprings), each of wich is under control of a sophisticated network of regulatory signals. The array of resporductive strategies among teleosts are extraordinarily diverse and, among them, those of syngnathids are peculiar. The Syngnathidae are a small family of brackish and freshwater species. They have attracted attention for decades due to their unique morphology, remarkable camouflage ability and the distinctive phenomenon of male pregnancy. Females deposit eggs, on or into a male incubation area, on the tail (subfamily Urophori) or on the trunk (subfamily Gastrophori). Syngnathids Mediterranean species only belong toSyngnathus,HippocampusandNerophis genera. Very little is known about the male reproductive biology and on the nervous system control on the reproductibe cycle of these fish. The aims of this work are therefore to clarify: 1) relevant aspects of male reproduction in both internal and external brooder species, such as those belongin toSyngnathusandHippocanous(Urophori) andNerophis genera(Gastrophori); 2) characterize the Dopaminergic system in adult and young males of the pipefish Syngnathus abaster. The aims have been achieved using different techniques of light, confocal and electron microscopy
"Sierotipizzazione e valutazione della sensibilità agli antibiotici di stipiti di Salmonella spp. isolati da carni suine al macello"
No full text“Metodi molecolari per la caratterizzazione e la tracciabilità di Listeria monocytogenes nei prodotti a base di carne”
No full textInvestigation of Salmonella enterica in Sardinian slaughter pigs: prevalence, serotype and genotype characterization
No full text"In order to improve the knowledge about the presence of Salmonella in pork meat in Sardinia (Italy), the prevalence and the sources of Salmonella at 5 pig slaughterhouses (slaughtered pigs and environment) were investigated and the isolates were characterised. A total of 462 samples were collected, 425 from pigs at slaughter and 41 from the slaughterhouse environment. Salmonella was isolated from 26\/85 (30.5%) mesenteric lymph nodes, 14\/85 (16.4%) colon contents, and from 12\/85 (14.1%) carcasses and livers. Salmonella prevalence was 38% (8\/21) in samples from surfaces not in contact with meat, and 35% (7\/20) in those from surfaces in contact with meat. Thirty-one pigs were identified as carriers of Salmonella in lymph nodes and\/or colon content, but of these, only 8 carcasses were positive. A total of 103 Salmonella isolates were serotyped and genotyped. Eight different serotypes were detected; the most common were S. Derby (44\/103, 42.7%) and S. Typhimurium (24\/103, 23.3%). The most prevalent S. Typhimurium phage type was DT193. Thirty-two isolates were found to be resistant to more than one antimicrobial (MDR). Pulse-field gel electrophoresis (PFGE) permitted the resolution of XbaI macrorestriction fragments of the Salmonella strains into 20 distinct pulsotypes. Combined application of a plasmid profiling assay (PPA) and PFGE gave useful additional information to assist in tracing the routes of Salmonella contamination in abattoirs. To reduce Salmonella prevalence some preventive measures should be encouraged: the origin of infected slaughter animals should be identified and direct and cross-contamination of carcasses should be avoided by adhering to HACCP principles in association with good hygiene procedures (GHP).
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Application of PFGE typing to trace the routes of Listeria monocytogenes contamination in “Salsiccia Sarda” processing plants: preliminary results
No full textVariations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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