1,721,081 research outputs found
Ciona robusta hemocyte populational dynamics and PO-dependent cytotoxic activity
No full textHemocyte populations from the ascidian Ciona robusta, separated through a Percoll discontinuous density gradient, are further characterized by May-Grünwald-Giemsa staining and a cytochemical reaction for phenoloxidase. Variability in cell density, acidophilic property and phenoloxidase activity suggest multiple hemocyte type populations, cell lineages and morphotypes that may be involved in distinct cellular responses. Therefore, unilocular refractile granulocytes, typical of this ascidian species, enriched in a fraction separated from the hemolymph show in vitro phenoloxidase-dependent cytotoxic activity against mammalian erythrocytes and a tumor cell lineage, in addition the properties listed above indicate relationships with vacuolated signet ring cells. Finally, bromo-deoxyuridine with, diamino-phenylindole fluorescent reaction and May-Grünwald-Giemsa staining show that in the hemolymph there are hyaline amoebocytes and granulocytes with potential proliferating activity. Present findings and reviewed images of previously reported inflammatory hemocytes in the tunic and pharynx allow us to speculate on theoretical outlines of hemocyte differentiation pathways
Hotel Las Arenas Balneario Resort: del uso popular al reuso exclusivo
No full textLas Arenas beach resort in Valencia: from the popular use to the exclusive re-use Las Arenas beach resort in the Malvarrosa beach was for many years a popular gathering spot during the summer season for the inhabitants of the city of Valencia. The bathing complex had a spa built in 1888, swimming pools, a restaurant and a cinema. It was constituted by two identical buildings, facing each other, with a row of marble columns surrounding the whole perimeter according to the classical architectural style. The beach resort was severely affected during the Spanish Civil War and one of the two buildings was completely destroyed. In the early 2000s, the bathing complex was fully restored and the destroyed pavilion was rebuilt as a perfect replica of the original one. In addition, a luxury four-storey hotel was built in the area between the two colonnaded buildings and the pool. This paper describes the lido renovation project highlighting how the wise use of materials and construction techniques
LA FOSSA DI SAN GUGLIELMO A CAGLIARI. Metodologie di lettura per il recupero e la valorizzazione del paesaggio urbano.
No full textThe paper proposes the study of an urban landscape set in a "fragile" urban environment. The area surveyed, is the so called "Fossa di San Guglielmo” in Cagliari. The site, involved since ancient times as a constant human presence, preserved until the beginning of the twentieth century interesting natural features. From the first half of the twentieth century, we observe the appearance of the first buildings related to activities for hospital care. In a short time they will be complemented by a series of design interventions that will compromise the delicate balance of the environment and landscape system. Through drawing and examination of historical maps, we want to read and represent the local current spatial configuration and the occurred transformations. The drawing shows the signs of permanence and the recent changes, marks that derive from physical and anthropogenic factors. The signs identified, are placed as the starting point of a path of knowledge for the recovery and enhancement of the urban landscape of this area
Osservazioni sul non finito nel recupero in architettura
Full text linkConservation of historical memory is usually neglected in current building renovation practice, which involves the
remodelling of plasterwork, floors, window frames and finishings in general. However, it does constitute the key element of certain renovation projects that have kept visible the traces of previous lives, rejecting the idea that new finishings should cancel all traces of the past.
Terms such as "unfinished" and "imperfect" concur with an attitude of openness to the possibility that the renovated building could be subject to new modifications, with knowledge of its past conditions offering great opportunities for the relative design. Moreover, the display of the untreated materials and the procedures undertaken corresponds to a frank, sincere, and truthful way of operating that may indeed have an ethical impact.
England and the Switzerland are the countries where you can find most cases of this tendency of refusal to smooth aver and camouflage. In these contexts we see the greatest evidence of that influence exercised by the neo-brutalist architecture of half a century ago, and by the As found tendency which, together, have contributed to mark the end of orthodoxy in the Modern Movement
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
La Valorizzazione del Paesaggio Culturale Italiano
No full textThe essay documents the state of research about the study of tools and methods to plan the preservation and enhancement of the Metropolitan Area of Rome. The research, after treating the southwestern suburbs of Rome, along the Tiber to include the archaeological sites of Ostia and Portus, currently is dedicated to the study of the Tiburtino territory, along the Aniene as far as Rome. The first topic of discussion in this new area of research is the Tivoli urban site. The study aims to describe and document traditional Tivoli building techniques to learn about the history and the culture material, in order to inform the technicians engaged in the practice of philological restoration. The research uses the historical-critical method, which is typical in the architectural restoration discipline; the product of the research, at this time, is a repertoire of drawings aimed to describe the architecture and the constructive lexicon. The mode of communication and management of this repertoire of cognitive data is a digital geographic system
Restauro e applicazioni informatiche. La gestione digitale della commessa per la documentazione e il miglioramento della qualità degli interventi di conservazione delle superfici architettoniche
No full textThe research project develops procedures and digital processes in order to enhance the design and the execution quality of interventions for the conservation of architectural surfaces. The aim is to finally promote the dialogue among disciplines with different natures but which are actually very close. The proposal consists in a mobile software based on the platform FileMaker for the management of restoration operations through the creation of a database organised in modules, permitting to register the analytic documentation of the entire restoration process. Moreover, the software allows to involve directly and at the same time the designer, the works supervisor, the construction company and the offices appointed for the preservation of historical buildings. These figures are called to participate directly and simultaneously in the decision procedure, in order to reduce the lenght of control and realization process in favor of a better cultural and technical quality, thanks to the implementation of “good practices” studied and developed with specific computer systems
RISARCIRE IL DANNO, RITROVARE UN SIMBOLO: IL PONTE E LA ROCCA DI CODIPONTE IN LUNIGIANA
No full textThe town of Codiponte, located in the inner part of Lunigiana (Tuscany), represents the typical historical centre perched on the limestone rocks of the Apennines, which have experienced, in the past, the importance of a strategic position and nowadays is in a progressive state of neglect. A recent seismic event, occurred in 2013, despite its moderate intensity, has damaged the two main symbols of the town: the bridge (which gives name to the town itself) and the castle, which suffered partial collapses, due to the lack of maintenance occurred in the last decades. This negative event was seen as an occasion of relaunch by the strong local community, aware of their architectural heritage and willing to promote strategies to enhance the area. The exposed project thus aims to recover the bridge and the Medieval fortress from the damages suffered and, through their preservation, to promote and enhance the neighboring area by encouraging tourism and the quality of life of the inhabitants. The project is based on an attentive survey and analysis of the current situation and aims, through minimal interventions, to give these symbolic constructions back to their inhabitants, increasing the safety and preservation conditions
Evolution, adaptation and immune functions of fish F-type lectins. The novelty of FBL from Trematomus bernacchii (Boulenger, 1902)
Full text linkLectins are a protein family, present in almost all living organisms and involved in different biological pathways, such as immune responses. The Fucose Binding Lectin (FBL), constitute the latest lectin family identified and characterized in fishes. The FBL family is constituted by a large number of proteins exhibiting multiples of the F-type motif, either tandemly arrayed or in mosaic combinations with other domains. In an early step a FBL has been isolated and characterized from serum of the Antarctic fish
Trematomus bernacchii by affinity chromatography on fucose-agarose column. A clear Bacterial agglutinating activity (BA) towards different bacteria strains (Escherichia coli, Kokuria rhizophyla and Bacillus subtilis) and Hemagglutinating activity (HA) toward rabbit erythrocytes was induced from the serum as well from the purified protein and thus confirm its involvement in host pathogen interactions. In SDS-PAGE analysis, the FBL exhibited an apparent molecular weight of 30 kDa. This data is confirmed from the sequence of the F lectin recognised on the T. bernacchii transcriptome. The sequence shows a similar and coherent structure with a supposed Mw 32.16 kDa and an isoelectropoint of 5.21. Furthermore, sequencing the N-terminus confirmed the identity of the sequence runned on SDS PAGE and blotted on PVDF membrane. The HA activity was analyzed at different temperatures and it was maintained also at the physiological living low temperatures of this fish habitat (close to 0 °C). Therefore, in order to identify trends linked to cold adaptation in Antarctic fish, we present our hypothesis on the conformational change determined by the aminoacidic substitutions respect the others fish fucolectins living in warmer water on the light of the general phylogenetic scenario including the new preliminary data on sharks FB
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