1,721,488 research outputs found
Le nostre storie sono i nostri orti, ma anche i nostri ghetti
No full textColloquio con il leader radicale italiano Marco Pannella nel quadro della politica italiana dal dopoguerra a oggi
Valutazione degli effetti del Dexametasone e del Cisplatino nei modelli sperimentali in vitro (OC-k3)e in vivo(Ratti Wistar)
No full textIn this thesis I want to evaluate the Dexamethasone and Cisplatin effects in vitro (OC-k3) and in vivo (Rats Wistar) model. On more I want to get the protocol for future gene therapy, using the new non-viral transposable element Sleeping Beauty in vitro model (Human Fetal Auditory Stem Cells (hFASC).
The Cisplatin is an antineoplastic agent used to contrast different types of malignant tumors (testicolaris, ovaric, bladder, neck and head). This drug is very toxic and it generates many side effects like ototoxicity. This agent destroys the inner ear tissues, but they are not able to regenerate for this manner it is very important to study the otoprotective drugs mechanisms. There are many agents otoprotective and I want to remember the Dexamethasone. Dexamethasone is a glucocorticoid and usually it is used to contrast the inflammatory processes.
The aim of this thesis is evaluating the drugs effects and protective effect of Dexamethasone against the Cisplatin toxicity.
I used the cell line OC-k3 for in vitro model, because these cells derive from immortal mouse inner ear. The OC-k3 don’t show the neurological markers, but epidermal markers.
For evaluating the drugs toxicity I used the flow citometry and I noted that the Cisplatin toxicity is time dependent. Dexamethasone is not toxic and is able to protect the cells from Cisplatin action.
Another method for toxicity and protection evaluation is studying the cytoskeleton morphology.
I noted that after 24h Cisplatin and Dexamethasone are not toxic for OC-k3, but after 48h Cisplatin destroys the cytoskeleton, while Dexamethasone is not toxic and protects the cells from Cisplatin action.
Finally I studied the apoptosis marker after Cisplatin and Dexamethasone treatment.
Cisplatin generates apoptosi intrinsecal via, but Dexamethasone is not toxic and it protects the cells, because they don’t show the apoptosis marker.
I can conclude that Cisplatin is toxic for OC-k3 and Dexamethasone is not toxic and it is able to protect the cells from Cisplatin action.
In vivo model I used the Wistar rats and I injected the drugs introperitoneal via alone and in co-treatmennt.
To evaluate the drugs effects I used the microscope (SEM) and Immunoistochemistry.
I noted that Cisplatin destroys the inner ear tissues and Dexamethasone is not toxic and protects the tissues from Cisplatin action.
In vitro and in vivo model, Cisplatinand Dexamethasone generate the same effects; Cisplatin is toxic as for the cells as for the tissues.
Dexamethasone is not toxic and it is protective as for cells as for tissues.
For gene therapy I used hFASC and I trasfected them with new non viral transposable element: Sleeping Beauty. Two plasmids derive from this transposable element (pT2/veus: gene for GFP, SB100: gene for transposase).
I used different concentrations of these plasmids for evaluating their toxicity, I noted that high concentrations of plasmids are toxic for the cells and the green cells number decreases during the incubation time. I decided to decrease the plasmids concentrations and I noted that the green cells number increases.
I can conclude that high concentrations of plasmids are toxic for the cells, but I can transfect the cells without problem if I use low concentrations.
After I evaluate if the cells are able to express the typical marker of undifferentiated cells (SOX 2, PAX 2, OCT4). With immunoistochemistry I noted the cells show these typical markers. Finally I decided to differentiate the transfected cells to bipolar neurons and cells like hair cells. I got that no transfected cells are able to differentiate to neuronal cells and cells like hair cells only. It is not significant result because I had one only single cell transfected in that cells pool
Influenza degli ormoni maschili e femminili sull'insorgenza e sviluppo dei tumori sperimentali da 20-metilcolantrene in ratti albini di giovane età
No full textIN VITRO PROTECTIVE EFFECTS OF DEXAMETHASONE AGAINST CISPLATIN OTOTOXICITY IN OCK3 MOUSE CELL LINE.
No full textPresent therapies of malignant tumours are still based on cytotoxic drugs that damage DNA or inhibit cell division, causing death of cancerous cells and tissues. However, most chemiotherapeutic drugs are non-specific, thus their cytoxic action also affects healthy cells and tissues. One of the most widely employed chemiotherapic drug, cisplatin, shows serious ototoxic effects leading to more or less profound hearing loss. To counteract these effects, several strategies have been devised, such as the administration of antioxidants and glucocorticoids. Dexamethasone, a glucocorticoid widely employed as anti-inflammatory drug, is known to exert some protective effects against cisplatin toxicity. This study was aimed to test by cytofluorimetry the time/dose effects of dexamethasone and cisplatin, administered separately and in co-treatment, in an inner ear mouse cell line (OCK3). The results showed that, in the experimental conditions tested, dexamethasone had no cytotoxic effects at any concentration, while cisplatin had time/dose dependent cytotoxicity. Concerning the co-treatment, when OCK3 cells were pre-treated with dexamethasone before cisplatin administration, a lower cell mortality was observed at 48 h incubation time and concentrations between 50 and 250 nM. Thus dexamethasone apparently exerts in vitro protective effects against cisplatin-induced ototoxicity
European Court of Human Rights : Associazione Politica Nazionale Lista Marco Pannella and Radicali Italiani v. Italy and Associazione Politica Nazionale Lista Marco Pannella v. Italy
No full textOn 31 August 2021 the European Court of Human Rights (ECtHR) has delivered two judgments dealing with political pluralism in programmes on public television (RAI). In both cases the ECtHR emphasized the need for pluralism in the news and current affairs programmes and in the political platform programmes on the public broadcaster. In the first case (Associazione Politica Nazionale Lista Marco Pannella and Radicali Italiani v. Italy) the ECtHR found no violation of the right to freedom of expression of a political association who complained about the discontinuance of the political platform programmes on RAI since 2008. In the second case (Associazione Politica Nazionale Lista Marco Pannella v. Italy) the ECtHR found a violation of the political association’s right to freedom of expression as guaranteed by Article 10 of the European Convention on Human Rights (ECHR). In this case the ECtHR found that the applicant association had been excluded from, or at least highly marginalized in media coverage of political debate, as on three occasions they had been excluded from taking part in very popular current-affairs television programmes on the RAI
An Extended Relational Data Model for Multimedia Databases
No full textWe have extended the canonical relational data model to enable the management of multimedia objects. In an attempt to provide a smooth paradigm shift to multimedia information system development, we have enhanced the relational data model framework with techniques for modeling, storing and manipulating multimedia data. In particular, we have provided a graphical conceptual model for structuring a multimedia
document and mapping rules for translating it into an extended relational data schema. Extensions have regarded the management of foreign keys, active components, mechanisms
for the management of spatial and temporal relations, and finally functions for handling multimedia presentations. As a consequence, we have also provided extensions
to the SQL language to handle these new mechanisms
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
INTRACLUSTER STELLAR POPULATION PROPERTIES FROM N-BODY COSMOLOGICAL SIMULATIONS. I. CONSTRAINTS AT Z = 0
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