1,721,193 research outputs found
The evolution of extracellular hemoglobins of annelids, vestimentiferans, and pogonophorans.
No full textThe evolution of extracellular hemoglobins of annelids, vestimentiferans, and pogonophorans was investigated by applying cladistic and distance-based approaches to reconstruct the phylogenetic relationships of this group of respiratory pigments. We performed this study using the aligned sequences of globin and linker chains that are the constituents of these complex molecules. Three novel globin and two novel linker chains of Sabella spallanzanii described in an accompanying paper (Pallavicini, A., Negrisolo, E., Barbato, R., Dewilde, S., Ghiretti-Magaldi, A., Moens, L., and Lanfranchi, G. (2001) J. Biol. Chem. 276, 26384--26390) were also included. Our results allowed us to test previous hypotheses on the evolutionary pathways of these proteins and to formulate a new most parsimonious model of molecular evolution. According to this novel model, the genes coding for the polypeptides forming these composite molecules were already present in the common ancestor of annelids, vestimentiferans, and pogonophorans
Exploring the immune resilience of Mediterranean mussels: Recent advances and future directions
Full text linkThe Mediterranean mussel (Mytilus galloprovincialis) is a key species in European aquaculture, known for its economic and societal importance, particularly as a primary source of income for local fisheries in European coastal areas. While historically resilient to the mass mortality events that have affected other bivalve species, M. galloprovincialis may face increasing threats from emerging pathogens, including bacteria, viruses, and eukaryotic parasites. These microorganisms, often opportunistic, pose heightened risks in the current climate change scenario, where heatwaves are becoming increasingly frequent and the persistent presence of pollutants is suspected to impair the functional response of hemocytes. Over the past decade, significant advancements in immunological research have provided deeper insights into the cellular and molecular mechanisms underlying the robust defense system of M. galloprovincialis, which allows this species to efficiently cope with a broad range of infections. By analyzing the scientific literature published on mussel immunology over the past ten years, this review consolidates current knowledge on the immune system of the Mediterranean mussel. We place a particular focus on the cellular and molecular components involved in the recognition and elimination of microbial pathogens and discuss how the most recent discoveries may inform improved management and disease mitigation strategies for Mediterranean mussel farming in the in the years to come
Relevance of plant and fungal DNA metabarcoding in aerobiology
No full textAerobiology studies biological particles present in the air of both indoor and outdoor environments. In the past few years, the application of molecular techniques has considerably improved the knowledge of organismal diversity in air samples and highlighted its ecological and clinical importance. DNA metabarcoding in particular has been recently applied worldwide to describe the biological composition of airborne bacteria, fungi and plants. Here, we provide a comprehensive overview of those aerobiology studies which, in the past few years, have applied DNA metabarcoding techniques. We focus specifically on researches including plants and fungi and review the multiplicity of experimental plans, sampling procedures, laboratory processing methods, the choice of the DNA barcode(s), the selection of sequencing platforms and bioinformatics pipelines for the comparability of data obtained from multiple origins
The Evolution of Extracellular Hemoglobins of Annelids, Vestimentiferans, and Pogonophorans.
No full textThe evolution of extracellular hemoglobins of annelids, vestimentiferans, and pogonophorans was investigated by applying cladistic and distance-based approaches to reconstruct the phylogenetic relationships of this group of respiratory pigments. We performed this study using the aligned sequences of globin and linker chains that are the constituents of these complex molecules. Three novel globin and two novel linker chains of Sabella spallanzanii described in an accompanying paper (Pallavicini, A., Negrisolo, E., Barbato, R., Dewilde, S., Ghiretti-Magaldi, A., Moens, L., and Lanfranchi, G. (2001) J. Biol. Chem. 276, 26384--26390) were also included. Our results allowed us to test previous hypotheses on the evolutionary pathways of these proteins and to formulate a new most parsimonious model of molecular evolution. According to this novel model, the genes coding for the polypeptides forming these composite molecules were already present in the common ancestor of annelids, vestimentiferans, and pogonophorans
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Comparative sequence analysis of antimicrobial peptides in Mytilus galloprovincialis and Ruditapes philippinarum
No full textVariations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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