1,190 research outputs found
Brief von Gerhard Bartl an Konstantin Langhaider (16.04.1770)
No full textTitel fingiertIncipit: Dum P[atrem] Innocentium Förtsch Carinthu
Molluginaceae Bartl.
No full text<p> 309. <b>Molluginaceae</b> Bartl. in F. G. Bartling & H. L. Wendland, <i>Beitr</i>. <i>Bot</i>. 2: 158. Dec 1825, <i>nom</i>. <i>cons</i>. <i>Adenogrammaceae</i> Nakai, <i>J</i>. <i>Jap</i>. <i>Bot</i>. 18: 101. 10 Mar 1942</p> <p> <i>Glinaceae</i> Mart., <i>Consp</i>. <i>Regn</i>. <i>Veg</i>.: 64. Sep–Oct 1835</p> <p> <i>Pharnaceaceae</i> Martinov, <i>Tekhno-Bot</i>. <i>Slovar</i>: 477. 3 Aug 1820</p> <p> <i>Polpodaceae</i> Nakai, <i>J</i>. <i>Jap</i>. <i>Bot</i>. 18: 102. 10 Mar 1942</p> <p> <i>Telephiaceae</i> Martinov, <i>Tekhno-Bot</i>. <i>Slovar</i>: 633. 3 Aug 1820</p>Published as part of <i>Reveal, James L., Chase, Mark W., Iii, - Apg & Iii, Apg, 2011, APG III: Bibliographical Information and Synonymy of Magnoliidae Abstract Kew words Introduction, pp. 71-134 in Phytotaxa 19 (1)</i> on page 101, DOI: 10.11646/phytotaxa.19.1.4, <a href="http://zenodo.org/record/10121353">http://zenodo.org/record/10121353</a>
Brief von Gerhard Bartl an Konstantin Langhaider (02.07.1773)
No full textTitel fingiertIncipit: Das mein lieber P. Bed
Brief von Gerhard Bartl an Konstantin Langhaider (02.07.1773)
No full textTitel fingiertIncipit: Das mein lieber P. Bed
Brief von Gerhard Bartl an Konstantin Langhaider (16.04.1770)
No full textTitel fingiertIncipit: Dum P[atrem] Innocentium Förtsch Carinthu
The influence of red blood cell storage on complement activation
No full textEINLEITUNG: Lagerschäden in Erythrozytenkonzentraten sind vielfältig und ein
limitierender Faktor in der Transfusionsmedizin. Welche Rolle Komplement in
diesem Zusammenhang einnimmt, wurde bislang nicht hinreichend untersucht.
METHODIK: Erythrozytenkonzentrate wurden 42 Tage gelagert, dabei wöchentlich
Proben entnommen und diese durchflusszytometrisch auf CD35, CD55, CD47, CD59
und C3d untersucht. Des Weiteren erfolgte ein direkter Antiglobulintest auf
C3d und die Immunglobuline IgA, IgG und IgM. Um den Kontakt des
Erythrozytenkonzentrats mit Serum zu Simulieren, wurde eine
Transfusionssimulation durchgeführt, wobei Erythrozyten für 30 Minuten bei 37°
mit homologem Serum inkubiert wurden. ERGEBNISSE: C3d stieg über die
Lagerungszeit an, wobei die Zunahme nach Transfusionsimulation stärker war
(ANOVA < 0.0001) als vor Transfusionsimulation (ANOVA p = 0.0103). Im direkten
Agglutinations Test wurden diese Beobachtungen bestätigt. Die Abnahme von
CD35, CD55 und CD47 über den Zeitraum der Lagerung war signifikant (jeweils
ANOVA p < 0.0001), wobei die meisten Veränderungen ab Tag 14 auftraten. CD59
zeigte keinen Abfall, und Immunglobuline wurden nicht nachgewiesen, unabhängig
der Transfusionssimulation. ZUSAMMENFASSUNG: Die Transfusion macht
Erythrozyten sensibler für spontane Komplementaktivierung. Ursächlich hierfür
könnte die zunehmende Anfälligkeit von CD35 gegenüber proteolytischer Spaltung
oder Vesikularisierung sein. Die Transfusion von Erythrozytenkonzentraten
jenseits einer Lagerungszeit von 14 Tagen könnte in kritisch kranken Patienten
durchaus relevant sein.BACKGROUND: Storage lesions in Red Blood Cell Concentrates are manifold and a
limiting factor within transfusion medicine. Up to now the role of complement
within this context has not sufficiently been researched. STUDY DESIGN AND
METHODS: Red Blood Cell Concentrates were stored for 42 days, with samples
being retrieved on a weekly basis. Such samples were examined by flow
cytometry for CD35, CD55, CD47, CD59 and C3d. In addition a direct
Agglutination Test was carried out for C3d and the Immunglobulins IgA, IgG and
IgM. To simulate the contact of Red Blood Cell Concentrates with serum, a
transfusion was simulated by incubating erythrocytes with homologue serum for
30 minutes at 37 degree Celsius. RESULTS: C3d increased during the storage
period. The increase was stronger after the transfusion was simulated (ANOVA <
0.0001) than before the transfusion was simulated (ANOVA p = 0.0103). The
direct Agglutination test confirmed these observations. The decline in CD35,
CD55 and CD47 during the storage period was significant (in each case ANOVA p
< 0.0001), with the largest changes arising after 14 days. CD59 showed no
decline and there were no Immunoglobulins detected, irrespective of the
transfusion simulation. CONCLUSION: RBCs are becoming increasingly following
TS, which might be associated with the increased susceptibility of CD35 to
proteolytic cleavage and vesiculation. The use of RBCs stored longer than 14
days might, in fact, be clinically significant in critically ill patients
Systematic assessment of psychopathology in sporadic Creutzfeldt-Jakob disease and possible associations with genetic polymorphism and clinical variables
No full textAnalysis of the arrangement of the binding pocket of the MDR pump Cdr1p of the pathogenic yeast Candida albicans - a major contributor to clinical drug resistance.
No full textNázev práce: Analýza uspořádaní vazebné kapsy MDR pumpy Cdr1p patogenní kvasinky Candida albicans - hlavního přispěvatele ke klinické lékové rezistenci Autor: Tomáš Bartl Katedra / Ústav: Fyzikální ústav UK Vedoucí diplomové práce: doc. RNDr. Dana Gášková, CSc., Fyzikální ústav UK Abstrakt: Kandidózové infekce jsou častou příčinou úmrtí hospitalizovaných pacientů. U nejvýznamnějšího kvasinkového patogenu, Candida albicans, je za jeho klinicky významnou rezistenci vůči používaným lékům (azolům) zodpovědná nadprodukce proteinů transportních pump, které léčivé látky odstraňují z buněk, a vzniká tak fenomén mnohačetné lékové rezistence - MDR. Cílem této práce bylo ověřit vhodnost použití kmenů kvasinky Saccharomyces cerevisiae využívajících heterologní expresi MDR pump z patogenní kvasinky Candida albicans, a na tomto systému potvrdit výsledky získané přímo na C. albicans s homologní expresí. Skutečnost, že azolová léčiva mikonazol, bifonazol a ketokonazol, a potenciometrická fluorescenční sonda diS-C3(3), jsou substráty pump CaCdr1p a CaCdr2p, ale nikoliv, nebo minimálně, pumpy CaMdr1p, tedy odpovídají dříve publikovaným výsledkům. Za pomoci jednak zónových testů, jednak diS-C3(3) fluorescenční metody, byla studována vazebná kapsa CaCdr1p s využitím strukturně podobných látek vorikonazolu, flukonazolu,...Title: Analysis of the arrangement of the binding pocket of the MDR pump Cdr1p of the pathogenic yeast Candida albicans - a major contributor to clinical drug resistance. Author: Tomáš Bartl Department: Institute of Physics of Charles University Supervisor: doc. RNDr. Dana Gášková, CSc., Institute of Physics of Charles University Abstract: Candida infections are becoming an increasing cause of death in hospitalized patients. The main reason for drug resistance in the most common yeast pathogen Candida albicans is an increased production of transport proteins, which are removing the drug from the cell cytosol and thus producing the phenomenon called multidrug resistance - MDR. The goal of this thesis was to verify the suitability of the yeast strain Saccharomyces cerevisiae with heterologously expressed MDR pumps from the pathogenic yeast Candida albicans and comparison to the results from C. albicans with homologous expression. The results that the azole drugs miconazole, bifonazole, and ketoconazole, together with potentiometric fluorescent probe diS-C3(3), are substrates of the CaCdr1p and CaCdr2p pumps, but not, or minimally, of the pump CaMdr1p, are consistent with the previously published work. The binding pocket of CaCdr1p was explored using the disc diffusion assay and the diS-C3(3) fluorescent probe...Institute of Physics of Charles UniversityFyzikální ústav UKMatematicko-fyzikální fakultaFaculty of Mathematics and Physic
The Correlation Between TP53 Expression and Ki-67 Proliferation with Bartl Malignancy Degree of Plasma Cell Neoplasm
No full textBACKGROUND: Plasma cell neoplasm (PCN) is a neoplastic plasma cell proliferation which includes solitary bone plasmacytoma (SBP), extramedullary plasmacytoma (EMP) and multiple myeloma (MM). Bartl classifies the degrees of PCN as low, intermediate and high. The aim of this study is to find the correlation between tumor suppressor gene p53 (TP53) expression and Ki-67 proliferation with Bartl Malignancy degree of PCN. Therefore earlier PCN diagnostic method to prevent the development of PCN into MM can be found.METHODS: Thirty-two PCN cases were classified into three groups based on Bartl's degrees of Malignancy. TP53 and Ki-67 immunohistochemical staining were performed on samples and the percentage of positivity was evaluated.RESULTS: The Bartl's low degree of Malignancy was found in 10 MM cases (31.2%), intermediate degree in 5 SBP cases (15.6%) and high in 2 SBP and EMP cases (6.2%). TP53 expression was obtainable at 4% of low, 16% of intermediate and 10% of high degree. There was a significant difference between TP53 expression in low and intermediate degree (p=0.004). Mean proliferation index of Ki-67 was 57% in low, 44.6% in intermediate, and 32.6% in high degree. There was no significant difference of Ki-67 proliferation indexes among the group (p=0.339).CONCLUSION: Increasing expression TP53 was in accord with Bartl's degrees of Malignancy, especially in low and intermediate degree, but there was no significant difference between Ki-67 proliferation index and Bartl's degrees ofmalignancy
Study of membrane transport processes in yeast using potentiometric fluorescent porbe diS-C3(3)
No full text1 Title: Study of membrane transport processes in yeast using potentiometric fluorescent probe diS-C3(3) Author: Tomáš Bartl Department: Institute of Physics of Charles University Supervisor: doc. RNDr. Dana Gášková, CSc., Institute of Physics of Charles University Abstract: Yeast membranes contain a number of transporters. Some are responsible for flow of nutrients to the inside of the cell, others for disposing of waste and foreign substances and some for transport of small ions or protons across the membrane. The focus of this work is on the activity of specific transport membrane proteins, so-called MDR pumps, which are responsible for transport of foreign substances or drugs, out of the cell. Using the series of mutant strains of the yeast Saccharomyces cerevisiae (AD1-3, AD1-8 and AD12) differentiated in the presence of specific MDR pumps in their membrane, an influence of various chemical substances on the intracellular concentration of the potentiometric fluorescent probe diS-C3(3), which is actively being transported out of the cell by some of the MDR pumps, was observed. By the examination of the effect of 2-deoxyglucose we proved the active contribution of not only the main MDR pump, Pdr5p, but also of some other pumps, in lowering the intracellular probe concentration. It was observed that..
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