1,013 research outputs found
Leis e planos urbanos na produção da cidade: o caso de Chapecó, SC
No full textDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-graduação em Urbanismo, História e Arquitetura da CidadeO presente trabalho discute a relação entre as Leis e Planos Urbanos e a organização espacial das cidades. Para isso, desenvolve a análise do Plano de Desenvolvimento Urbano de Chapecó, no Oeste de Santa Catarina, da metade da década de 70 e da Lei de Zoneamento aprovada no início da década de 80. O estudo objetiva compreender, à luz das condições sociais, políticas, econômicas de cada período, que tipo de repercussões espaciais essas Leis e Planos provocaram no espaço urbano de Chapecó, identificando seu papel como indutor de transformações urbanas no município, aliados aos interesses dos demais agentes urbanos, principalmente do capital agroindustrial. Sendo assim, a partir do estudo de um caso concreto relacionando os Planos e Leis Urbanas e sua proposta de intervenção em uma realidade urbana determinada, o trabalho contribui para a discussão dos alcances, limites, potencialidades e, principalmente, as repercussões espaciais atribuídas aos Planos e Leis Urbanas no espaço urbano, dando maior ênfase aos aspectos da distribuição sócio-espacial da população. The present work discusses the relationship between the Urban Laws and Plans and the spatial organization of the cities. For this, it develops the analysis of the Urban Development Planning of Chapecó, in the West of Santa Catarina, from the half of the 70´s and the approved Zoning Law at the beginning of the 80´s. The study aims to understand what kind of spatial consequences these Laws and Plans had provoked in the urban space of Chapecó, considering the social conditions, politics and economy of each period, identifying its roll as an inductive agent of urban transformations in the city, along with the interests of others urban agents, mainly the agroindustrial capital one. This way, from the study of a concrete case relating the Plans and Urban Laws and its proposal of intervention in a determined urban reality, the work contribute for the discussion of the reach, limits, potentialities and, mainly, the spatial consequences attributed to the Plans and Urban Laws in the urban space, putting more emphasis on the aspects of the partner-spatial distribution of the population
Remy Ambühl: Prisoners of War in the Hundred Year War: Ransom Culture in the Late Middle Ages, Cambridge, Cambridge University Press, 2013.
No full textReseña de Remy AMBÜHL: Prisoners of War in the Hundred Year War: Ransom Culture in the Late Middle Ages, Cambridge, Cambridge University Press, 2013 (ISBN: 9781107010949). A cargo de Alberto Reche Ontillera
Optimization and analisys of vaccination shedules using Simulated Annealing and Agent Based Models
No full textNutrients and organic matter reduction by sea cucmber in marine miltireopci aquaculture systems
No full textTesis Univ. Granada. Programa Oficial de Doctorado en Bioquímica y Biología MolecularThis research was supported by the projects CEI BioTic P-BS-46 of the University of Granada and CGL2014–52362R of the Spanish Ministry of Economy and Competitiveness and FEDER funds to Isabel Reche, a PhD fellowship from the Iranian Ministry of Science to Seyed Mohammad Sadeghi Nassaj. The time series and the experiments were performed at the iMare Natural S.L. facilities
The combination of artificial intelligence and systems biology for intelligent vaccine design
No full textIntroduction: A new body of evidence depicts the applications of artificial intelligence and systems biology in vaccine design and development. The combination of both approaches shall revolutionize healthcare, accelerating clinical trial processes and reducing the costs and time involved in drug research and development. Areas covered: This review explores the basics of artificial intelligence and systems biology approaches in the vaccine development pipeline. The topics include a detailed description of epitope prediction tools for designing epitope-based vaccines and agent-based models for immune system response prediction, along with a focus on their potentiality to facilitate clinical trial phases. Expert opinion: Artificial intelligence and systems biology offer the opportunity to avoid the inefficiencies and failures that arise in the classical vaccine development pipeline. One promising solution is the combination of both methodologies in a multiscale perspective through an accurate pipeline. We are entering an ‘in silico era’ in which scientific partnerships, including a more and more increasing creation of an ‘ecosystem’ of collaboration and multidisciplinary approach, are relevant for addressing the long and risky road of vaccine discovery and development. In this context, regulatory guidance should be developed to qualify the in silico trials as evidence for intelligent vaccine development
G-Quadruplex DNA as a Target in Pathogenic Bacteria: Efficacy of an Extended Naphthalene Diimide Ligand and Its Mode of Action
No full textGuanidine DNA quadruplex (G4-DNA) structures convey a distinctive layer of epigenetic information that is critical for regulating key biological activities and processes as transcription, replication, and repair in living cells. The information regarding their role and use as therapeutic drug targets in bacteria is still scarce. Here, we tested the biological activity of a G4-DNA ligand library, based on the naphthalene diimide (NDI) pharmacophore, against both Gram-positive and Gram-negative bacteria. For the best compound identified, NDI-10, a different action mechanism was described for Gram-positive or negative bacteria. This asymmetric activity profile could be related to the different prevalence of putative G4-DNA structures in each group, the influence that they can exert on gene expression, and the different roles of the G4 structures in these bacteria, which seem to promote transcription in Gram-positive bacteria and repress transcription in Gram-negatives
Anxiety and depressive symptoms among youth in Italy, Spain, and Portugal: A three-year post-pandemic study
No full textThe COVID-19 pandemic disrupted the population's lives. Stressful conditions during the lockdown and the reintroduction to a changed social environment emotionally affected children and adolescents. The aim of this work was to study anxiety and depressive symptoms in Italian, Spanish, and Portuguese children and adolescents aged 3 to 18 years at different moments of the COVID-19 pandemic: April 2020 (during confinement), September 2020 (with the schools' reopening), and September 2023 (with the situation restored). Parents of 1,097 children participated in at least one assessment, completing measures of child emotional symptoms online. Cases with subclinical symptoms of anxiety and depression were higher compared to pre-pandemic studies. Overall, anxiety increased from April 2020 to September 2020, decreasing in September 2023 with no differences compared to the first assessment. Depression was high in April 2020 but decreased in September 2020, with no significant differences three years later, in September 2023. Cross-country comparisons at each point are discussed. Moreover, boys showed higher levels of depression during the pandemic compared to girls. Older children, compared to younger ones, had more anxiety and depressive symptoms throughout all the moments. These findings highlight the emotional impact of the pandemic and its conditions on children and adolescents
Thiosugar naphthalene diimide conjugates: G-quadruplex ligands with antiparasitic and anticancer activity
No full textGlycosyl conjugation to drugs is a strategy being used to take advantage of glucose transporters (GLUT) overexpression in cancer cells in comparison with non-cancerous cells. Its extension to the conjugation of drugs to thiosugars tries to exploit their higher biostability when compared to O-glycosides. Here, we have synthesized a series of thiosugar naphthalene diimide conjugates as G-quadruplex ligands and have explored modifications of the amino sidechain comparing dimethyl amino and morpholino groups. Then, we studied their antiproliferative activity in colon cancer cells, and their antiparasitic activity in T. brucei and L. major parasites, together with their ability to bind quadruplexes and their cellular uptake and location. We observed higher toxicity for the sugar-NDI-NMe2 derivatives than for the sugar-NDI-morph compounds, both in mammalian cells and in parasites. Our experiments indicate that a less efficient binding to quadruplexes and a worse cellular uptake of the carb-NDI-morph derivatives could be the reasons for these differences. We found small variations in cytotoxicity between O-carb-NDIs and S-carb-NDIs, except against non-cancerous human fibroblasts MRC-5, where thiosugar-NDIs tend to be less toxic. This leads to a notable selectivity for β-thiomaltosyl-NDI-NMe2 12 (9.8 fold), with an IC50 of 0.3 μM against HT-29 cells. Finally, the antiparasitic activity observed for the carb-NDI-NMe2 derivatives against T. brucei was in the nanomolar range with a good selectivity index in the range of 30- to 69- fold
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