1,169 research outputs found
INTERKOMMUNALT RENSEANLEGG I HAMAR FORSØK I HALVTEKNISK MÅLESTOKK. Delrapport nr. 2: Forsøk i biologisk-kjemisk rensing
No full textFORSØK I HALVTEKNISK MÅLESTOKK MED BIOLOGISK-KJEMISK RENSING AV KOMMUNALT AVLØPSVANN. ALUMINIUMSULFAT, KALK OG JERN(2)-SULFAT + KALK BENYTTET SOM FELLINGSMIDDEL. RESULTATER FRA FORSØKENE MED VURDERIN
Forskningsprogrammet for rensing av avløpsvann PRA. Avsluttende rapport fra prosjektkomiteen
No full textForskningsprogram for rensing av avløpsvann kom i gang i 1970 på bakgrunn av Ressursutvalgets innstilling nr. 1. Hovedhensikten med programmet har vært å fremskaffe bedre grunnlag for de betydelige investeringer som var forestående på avløpssektoren for å redusere vannforurensningsproblemene. Den forskningsmessige delen av programmet ble avsluttet i 1977, mens arbeidet med å omsette forskningsresultatene slik at de blir best mulig tilgjengelige for brukerne har pågått helt til slutten av 1979. Denne rapporten markerer avslutningen på forskningsprogrammet og er laget for å gi en kort oppsummering av hva som har vært gjennomført, hvilke resultater som er fremkommet og hvilke erfaringer som kan trekkes av den måten programmet har vært lagt opp.Prosjektkomiteen for rensing av avløpsvann PR
PRA 2.1 Forsøksanlegget på kjeller. Utført arbeid, oppnådde resultater, planer for videre arbeid samt oversikt over tilsvarende forskning i andre land
No full textUNDERSØKELSE AV DRIFTSFORHOLD VED MEKANISKE, BIOLOGISKE OG KJEMISKE RENSEANLEGG, FORSØK MED BIODAMMER, RENNEFORSØK OG SLAM 74 0244PRA Prosjektkomiteen for rensing av avløpsvan
NreB from Achromobacter xylosoxidans 31A is a nickel-induced transporter conferring nickel resistance
No full textThere are two distinct nickel resistance loci on plasmid pTOM9 from Achromobacter xylosoxidans 31A, ncc and nre. Expression of the nreB gene was specifically induced by nickel and conferred nickel resistance on both A. xylosoxidans 31A and Escherichia coli. E. coli cells expressing nreB showed reduced accumulation of Ni2+, suggesting that NreB mediated nickel efflux. The histidine-rich C-terminal region of NreB mas not essential but contributed to maximal Ni2+ resistance.NIGMS NIH HHS [R01 GM055425, R37 GM055425, GM 55425
Reactivation of a Trypanosoma cruzi Infection in a Rhesus Monkey (Macaca mulatta) Experimentally Infected with SIV
No full textTrypanosoma cruzi-like flagellates were incidentally noted in blood smears of a routinely monitored rhesus monkey experimentally infected with the simian immunodeficiency virus (SIV). Immunodeficiency in the course of the SIV infection reactivated a chronic infection of Chagas' disease that had been unnoticed when the macaque was imported to Europe. The animal developed no specific clinical symptoms of American trypanosomiasis, but histologically a chagasic myocarditis was detected. Analysis of the small subunit rRNA gene of the trypanosome identified the protozoan as T. cruzi
Gastrointestinal Pathology in Rhesus Monkeys with Experimental SIV Infection
No full textThe updated results of current pathomorphological investigations in SIV-infected rhesus monkeys (Macaca mulatta) are summarized. After experimental infection with several SIVmac251 subtypes and various vaccination trails, 147 rhesus monkeys were morphologically examined until now. The pathology of the gastrointestinal tract in SIV-infected animals resembled those of human cases with HIV and AIDS. Alterations were considered to be primary SIV-induced (SIV enteropathy, giant cell disease) or secondary caused by opportunistic agents. Typical secondary gastrointestinal opportunistic infectious agents were parasites (Cryptosporidium sp., Trichuris sp., Trichomonas sp., Spironucleus sp.), viruses (cytomegalovirus, adenovirus) and bacteria (Mycobacterium simiae). Five animals developed malignant lymphomas involving the intestinal tract. The present observations revealed that SIV infection of rhesus monkeys provide an excellent model for studies on the pathogenesis of HIV in man
SIV-associated Lymphomas in Rhesus Monkeys (Macaca mulatta) in Comparison with HIV-associated Lymphomas
No full textA retrospective study was performed to characterize malignant lymphomas of 16 Simian immunodeficiency virus (SIV)-infected rhesus monkeys ( Macaca mulatta), 2–9 years of age, on the basis of clinical data, histologic and immunophenotypic results, and cell death indices compiled with the TdT-mediated X-duTP nick end labeling method. We particularly focused on providing immunohistochemical evidence of expression products of EBNA2, Bcl2, c-Myc, P21, P53, and Bc16. Results were compared with data from the literature on human HIV-associated lymphomas. According to the updated Kiel classification, the lymphomas were classified as 11 centroblastic lymphomas, three immunoblastic lymphomas, one Burkitt-like lymphoma, and one immunocytoma. Using antibodies to CD20, the B-cell origin of tumor cells was demonstrated. SIV antigen was not demonstrated in the tumor cells. Infection with rhesus lymphocryptovirus was present in 94% of the monkeys. Lymphomas revealed expression of Bc12 in 15/16 (94%), c-Myc in 14/16 (88%), P21 in 10/16 (63%), P53 in 12/16 (75%), and Bc16 in 1/16 (6%) monkeys. This study provided evidence that the expression of these gene products, which are thought to play an important role in cell proliferation and apoptosis in HIV- and non-HIV-associated lymphomas, are also involved in the pathogenesis of lymphomas in SIVinfected rhesus monkeys. A tentative relationship between the described gene products and the cell death indices was established for the expression of Bc12. The present primate model represents a suitable animal model for studying the pathogenesis of AIDS-associated lymphomas
P. patens genomische und transkriptomische Analysen
No full textThe model organism Physcomitrium patens, formerly Physcomitrella patens is a moss in the
Funariaceae family. Due to P. patens ability to generate easily transgenic plants via homologous
recombination, the interest of scientists worldwide was attracted. P. patens was the world's first
completely sequenced non-seed plant genome (V1). Constant improvements of the genome assembly
and the associated gene annotations resulted in the current P. patens pseudo-chromosomal genome
version (V3). This genome version is the basis of all analyses performed in this thesis.
Since P. patens became a U.S. Department of Energy Joint Genome Institute (DOE JGI) plant flagship
genome 1 and a member of the JGI Gene Atlas project 2, hundreds of P. patens RNA-seq samples were
generated. During my time as a PhD student, I analysed the JGI Gene Atlas RNA-seq samples and
several dozen other RNA-seq samples from different projects. These RNA-seq samples contained data
from five different P. patens ecotypes/accessions (Gransden, Kaskaskia, Reute, Villersexel, and
Wisconsin).To efficiently analyse this data, I developed a powerful RNA-seq pipeline to perform
differentially expressed gene (DEG) calling. The performance of the RNA-seq pipeline was tested by
comparing its results to commercial software solutions and multiple RNA-seq samples from different
species.
My newly generated gene expression results, together with previous published expression data from
a variety of other projects, were stored at our novel online tool PEATmoss. Furthermore, my gene
version lookup tables were implemented in a database structure. This, allows PEATmoss users to find
gene models of different gene annotation versions and to use them in PEATmoss.
With an updated version of my RNA-seq pipeline, I identified and analysed sequence variations in P.
patens accessions. A clear clustering by individual accessions could be shown. I could demonstrate,
that due to decades of vegetative propagation in laboratories, somatic mutations have accumulated
in Gransden laboratory plants. In addition, we used restriction fragment length polymorphism (RFLP)
to offer a simple method for quick identification of unknown P. patens plants.
1 https://jgi.doe.gov/our-science/science-programs/plant-genomics/plant-flagship-genomes/
2 https://jgi.doe.gov/doe-jgi-plant-flagship-gene-atlas/Der in dieser Arbeit verwendete Modelorganismus Physcomitrium patens, früher Physcomitrella
patens (zu Deutsch Kleines Blasenmützenmoos), ist ein Laubmoos (Bryopsida) der Familie Funariaceae.
Aufgrund einer sehr effizienten homologen Rekombination wurden Wissenschaftler bereits früh auf
das Moos aufmerksam. P. patens war die erste Pflanze, außerhalb der Samenpflanzen, deren Genom
vollständig sequenziert wurde (V1). Durch kontinuierliches Voranbringen und Verbessern der
Genomassemblierung konnte diese in eine pseudo-chromosomale Struktur gegliedert werden (V3).
Diese V3-Genomversion ist die Basis aller in dieser Arbeit durchgeführt Analysen.
Mit was für einer Genantwort reagieren Organismen infolge eines induzierten Stresses? Diese und
weitere Fragen versucht das U.S. Department of Energy Joint Genome Institute (DOE JGI) im Rahmen
des Gene Atlas Projekts1 zu beantworten. In dessen Folge hunderte RNA-seq-Experimente
durchgeführt wurden. Diese P. patens JGI Gene Atlas Daten sowie duzende weiterer RNA-seq-
Experimente, unterschiedlichster Projekte, durfte ich in meiner Zeit als Doktorand analysieren. Die
gleichbleibend hohe Qualität und Effizienz der Datenanalyse konnte mittels einer neu entwickelten
RNA-seq-Pipeline gewährleistet werden, welche unter anderem zuverlässig differenziell exprimierte
Gene (DEG) detektiert. Die Leistungsfähigkeit der RNA-seq-Pipeline wurde in unterschiedlichsten
Projekten getestet und konnte sich auch gegen kommerzielle Software behaupten.
Die auf Basis meiner RNA-seq-Pipeline berechneten Expressionswerte wurden zusammen mit
Microarray-Expressionsdaten, aus bereits veröffentlichten Projekten, auf der für diesen Zweck neu
entwickelten Onlineplattform PEATmoss zur Verfügung gestellt. Benutzer können Expressionsdaten
unterschiedlichster Experimente interaktiv miteinander vergleichen und Resultate in übersichtlicher
Form herunterladen. Des Weiteren ermöglicht PEATmoss das Konvertieren aller bisher verwendeten
P. patens Genmodellversionen untereinander.
Die zuvor beschriebenen Sequenzdaten beinhalten fünf unterschiedliche P. patens Ökotypen
(Gransden, Kaskaskia, Reute, Villersexel und Wisconsin). Sequenzvariation unter den Ökotypen wurde
bereits in früheren Studien aufgezeigt. In dieser Arbeit wurden erstmals Sequenzvariationen für fünf
unterschiedliche Ökotypen auf Basis von RNA-seq-Daten untersucht. Hierfür wurde die RNA-seq-
Pipeline modifiziert und die Funktionalität auf Variationsdetektion ausgeweitet. Eine klare
Gruppierung der einzelnen Ökotypen und Gransden-Stämme kann beobachtet werden. Zusätzlich
stellen wir mittels Restriktionsfragmentlängenpolymorphismus (RFLP) eine Methode bereit, die eine
klare Identifikation einzelner P. patens Pflanzen in Laboren ermöglicht.
1 https://jgi.doe.gov/doe-jgi-plant-flagship-gene-atlas
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