698 research outputs found

    Reply to Alexandrova, et al. comment

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    A Reply to the Comment by O. Alexandrova, S. D. Bale, and C. Lacombe.https://journals.aps.org/prl/abstract/10.1103/PhysRevLett.111.14900

    Volvariella pulla E. F. Malysheva & A. V. Alexandrova, Phytotaxa

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    Volvariella pulla E.F. Malysheva & A.V. Alexandrova, Phytotaxa 408 (4): 247 (2019) (Figures 4 & 5) MycoBank MB 831060, Faces of Fungi 08024 Description:— Basidiome large. Pileus 70 mm diam., plano-convex with a low broad umbo, brownish grey (6E2) with slightly darker color at the center, dull, dry, non-hygrophanous, with fibrillose to appressed-squamulose surface; margin decurved to straight, crenulate; context light yellow (4A5), soft. Lamellae free, ventricose, close, moderately thin, soft, pale orange (6A3) to brownish orange (6C8) and turning light brown (6D8) with age; edge finely fimbriate, concolorous with faces; lamellulae present, very variable in length, mostly in one tier. Stipe 65 × 4 mm, central, terete, tapered from the base to apex, solid, dark brown (6F4–5), slightly paler near the base, entirely pubescent; context light yellow (4A5); annulus absent. Volva rather thick, mostly free, saccate, fragile, greyish brown (6D3). Spore print pinkish (7A2). Odor indistinct. Taste mild. Basidiospores [60,1,1] (5.5)6–7–7.5(8.0) × 4–4.4–4.5(5) µm (Q = 1.2–1.8, Q* = 1.5), broadly ellipsoid to ellipsoid, oblong, ovoid, thickened wall, hyaline in 5% KOH. Basidia [30,1,1] (16)17–18.1–19(19.5) × (7)7.5–8– 8.5(9) µm (Q = 1.8–2.8, Q* = 2.3), tetrasporic, narrowly to broadly clavate, hyaline in 5% KOH. Cheilocystidia [20,1,1] (24)29–37.5–56(87) × (9)11–12.1–13(17) µm (Q = 1.6–6.4, Q* = 2.8), abundant, varied in size and shape, narrowly to broadly utriform, broadly lageniform, broadly fusiform, lecythiform, thin-walled, hyaline and colorless in 5% KOH. Pleurocystidia [20,1,1] (25)29–36.5–44(56) × (6)12–16.6–19(32) µm (Q = 1.5–4, Q* = 2.2), ellipsoid, obpyriform, utriform, broadly fusiform, lageniform, mostly thin-walled, hyaline in 5% KOH. Hymenophoral trama inverse, made up of thin-walled hyaline hyphae. Pileipellis a trichoderm; terminal elements with light brown (6D5) intracellular pigment, slightly darker on the swollen ends, [20,1,1] 77–177 × 11–31 µm. Stipitipellis a cutis; terminal elements with light brown (6D8) intracellular pigment [20,1,1] 75–162 × 8–15 µm, forming hairs. Clamp connections not seen in any part. Habitat and distribution:—Solitary, on the soil among grasses partly covered with litter. First described from Gia Lai Province in Vietnam. Now it is also known from northern Thailand. Specimen examined:— THAILAND. Chiang Rai Province: Mae Fah Luang University, 20.0449° N, 99.8943° E, lawn, 27 September 2018, P. Sysouphanthong, MFLU 19-1534 (GenBank MT 074696 - ITS). Notes:—According to the protologue, Volvariella pulla has medium to large basidiomes, with greyish brown or ash brown pileus and colored volva; the stipe is beige, ochraceous or greyish brown at base (Malysheva et al. 2019). The specimen from Thailand is morphologically similar to the holotype. However, the stipe was darker (dark brown). This difference might be due to the more advanced maturity or drier condition of the specimen, which was photographed and described after getting back to the laboratory (Figure 4). Volvariella pulla is recorded for the first time from Thailand after it was described from Vietnam in 2019 (Malysheva et al. 2019). Although V. pulla is morphologically most similar to V. rostricystidiata and V. volvacea, it is phylogenetically most closely related to V. guttulosa E.F. Malysheva & A.V. Alexandrova (2019: 242) and V. niveosulcata E.F. Malysheva & A.V. Alexandrova (2019: 240) (Figure 1).Published as part of Niego, Allen Grace T., Sysouphanthong, Phongeun, Thongklang, Naritsada, Hyde, Kevin D., Phonemany, Monthien, Phookamsak, Rungtiwa & Raspé, Olivier, 2021, A new species of Volvariella and the first record of Volvariella pulla (Agaricales: incertae sedis) from Thailand, pp. 237-250 in Phytotaxa 480 (3) on pages 244-246, DOI: 10.11646/phytotaxa.480.3.3, http://zenodo.org/record/541688

    Testing for Pseudomonas syringae pv. atrofaciens and Xanthomonas campestris pathovars on cereals in Italy

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    During recent years, recurrent attacks of basal glume rot/leaf blight attributed to Pseudomonas syringae pv. atrofaciens have been observed on cultivars of durum wheat grown in the Po valley (IT). The main aim of this study was to test for this pathogen and Xanthomonas campestris pathovars in commercial seed lots of cereals produced in different regions of Italy, as well as in symptomatic plants collected in the field. None of the analyses led to the detection of xanthomonads. In testing for P.s. atrofaciens, representative bacterial isolates were selected and characterized; this was done by combining conventional identification tests and computerized densitometric analysis of electrophoretic patterns of cell proteins obtained using the SDS‐PAGE technique. P.s. atrofaciens was detected in seed samples (barley, wheat and durum wheat) and symptomatic plants (durum wheat) grown in northern and central Italy. The possible pathogenic role of other unrelated fluorescent pseudomonads in this interaction was also examined. Difficulties in discriminating pvs syringae and atrofaciens of P. syringae are discussed and the uncertain taxonomic relationship between these two pathovars is emphasized. Copyright © 1995, Wiley Blackwell. All rights reserve

    The phosphatidylserine receptor from <it>Hydra </it>is a nuclear protein with potential Fe(II) dependent oxygenase activity

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    Abstract Background Apoptotic cell death plays an essential part in embryogenesis, development and maintenance of tissue homeostasis in metazoan animals. The culmination of apoptosis in vivo is the phagocytosis of cellular corpses. One morphological characteristic of cells undergoing apoptosis is loss of plasma membrane phospholipid asymmetry and exposure of phosphatidylserine on the outer leaflet. Surface exposure of phosphatidylserine is recognised by a specific receptor (phosphatidylserine receptor, PSR) and is required for phagocytosis of apoptotic cells by macrophages and fibroblasts. Results We have cloned the PSR receptor from Hydra in order to investigate its function in this early metazoan. Bioinformatic analysis of the Hydra PSR protein structure revealed the presence of three nuclear localisation signals, an AT-hook like DNA binding motif and a putative 2-oxoglutarate (2OG)-and Fe(II)-dependent oxygenase activity. All of these features are conserved from human PSR to Hydra PSR. Expression of GFP tagged Hydra PSR in hydra cells revealed clear nuclear localisation. Deletion of one of the three NLS sequences strongly diminished nuclear localisation of the protein. Membrane localisation was never detected. Conclusions Our results suggest that Hydra PSR is a nuclear 2-oxoglutarate (2OG)-and Fe(II)-dependent oxygenase. This is in contrast with the proposed function of Hydra PSR as a cell surface receptor involved in the recognition of apoptotic cells displaying phosphatidylserine on their surface. The conservation of the protein from Hydra to human infers that our results also apply to PSR from higher animals.</p

    Maize Grain Yield response to n Fertilization, CliMate and HYbrids

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    abstract MikovA, A., P. AlexAndrovA and i. diMitrov, 2013. Maize grain yield response to n fertilization, climate and hybrids. Bulg. J. Agric. Sci., the relationship between soil-climate conditions, rate of nitrogen application, two maize hybrids and maize grain yield under continuous corn production and irrigation was investigated during 17 years period in eight soil climatic regions in Bulgaria. the experiment was monofactorial with four levels of n fertilization. Maize hybrids from two FAo groups (400 and 700) cultivated under irrigation as a monoculture were used. Statistical analyses were performed in order to describe some relations between maize yield and variables, connected with maize growth. According to the results from AnovA analysis factor &quot;n rate&quot; had the highest effect on the variability of maize yield (72.03 %). The influence of &quot;Hybrid&quot; and &quot;Station&quot; -environmental conditions (soils and climate) was quite low -9.51% and 2.01% respectively. Multiple regression analyses showed that n rate, temperature sum and total water use could be successfuly used as yield predictors

    Prediction of Two-Dimensional Phase of Boron with Anisotropic Electric Conductivity

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    Two-dimensional (2D) phases of boron are rare and unique. Here we report a new 2D all-boron phase (named the it phase) that can be grown on a W(110) surface. The pi phase, composed of four-membered rings and six-membered rings filled with an additional B atom, is predicted to be the most stable on this support. It is characterized by an outstanding stability upon exfoliation off of the W surface, and unusual electronic properties. The chemical bonding analysis reveals the metallic nature of this material, which can be attributed to the multicentered pi-bonds. Importantly, the calculated conductivity tensor is anisotropic, showing larger conductivity in the direction of the sheet that is in-line with the conjugated pi-bonds, and diminished in the direction where the pi-subsystems are connected by single sigma-bonds. The pi-phase can be viewed as an ultrastable web of aligned conducting boron wires, possibly of interest to applications in electronic devices.CSST Scholarship; Postdoctoral Fellowship of the Basque Country [POS 2015 1 0008]; NSF-CAREER [CHE1351968]; Department of Defense; UCLA-IDRE clusterSCI(E)ARTICLE61224-1228

    2D- compared 3D- human cell models: cytotoxic effects of humid lightweight coal ash derived from the disposal of waste

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    The presence of waste in environment represents a significant risk to huma health.. The potential toxic effects of humid lightweight coal ash (EWC100102) derived from waste disposal were investigated in vitro on human keratynocyte and andotelial cell lines, comparing different approac: direct/indirect contact, 2D/3D models.Cyotoxic potential resulted influenced by direct and indirect contact exposure; Endothelial cells resulted more susceptible to experimental exposure; 3D culture can provide a better microenvironment for mimic the real in vivo conditions, and are more sensiive and accurat tool for assessing in vitro potential of a chemical compound

    Multifractal Analysis of the Mechanical PropOKerties of the Texture of Biopolymer-Inorganic Composites of Chitosan-Silicon Dioxide

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    An original multifractal algorithm is proposed for calculating the fractal characteristics of the cluster texture of biopolymer-inorganic (BIN) composites from microphotographic images (MPIs) of their texture, which allows one to determine the quantitative dependence of the mechanical properties of biopolymer–inorganic composites on the characteristics of their cluster texture. As a result of the studies conducted on the MPI texture of the “chitosan-silicon dioxide” BIN composites using a multifractal algorithm, it was found that such important indicators as strength and plasticity can be quantitatively described using generalized fractal pixel dimensions of MPI samples of the BIN composite. It was established that when the amount of silicon dioxide in the samples of the BIN composite changes, the mechanical characteristics of the BIN composites, such as strength and plasticity, can be quantitatively described using generalized fractal pixel dimensions of the MPIs of the BIN composite samples

    Agricultural Academy

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    Abstract PoPova, T. P., R. I. alexandRova, R. Tudose, e.-M. MosoaRca and o. cosTIsoR, 2012. antimicrobial activity in vitro of four nickel complexes. Bulg. J. Agric. Sci., The in vitro effect of four nickel complexes with ligands containing the antipyrine moiety n,n&apos;-bis(4-antipyrylmethyl)-piperazine (BaMP) and n,n&apos;-tetra-(antipyryl-1-methyl)-1,2-diaminoethane (TaMen) against 33 pathogenic bacterial strains, as well as control ones, was examined. The antifungal activity of the complexes against four Candida spp. was established too. Two routine methods were used: of Bauer-Kirby and the determination of minimum inhibitory concentrations. The compounds ni(TaMen)(ncs) 2 and ni(TaMen)(clo 4 ) 2 manifested good antibacterial activity in vitro against the tested bacteria, especially towards the Gram-positive strains, as well as against K. pneumoniae and P. aeruginosa. among all tested microorganisms, the sensitivity of the strains of E. coli to the examined complexes proved most weak. Candida spp. showed high sensitivity to ni(TaMen)(ncs) 2 and ni(TaMen)(clo 4 ) 2 too and weaker to ni 2 (BaMP)(cl) 4 and ni 2 (BaMP)(ac) 4
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