170,121 research outputs found
Chlamydomonas palmellomoewusii Novis & Visnovsky 2012, sp. nov.
No full textChlamydomonas palmellomoewusii Novis & Visnovsky, sp. nov. (Figs 1A–D) Cellulae juvenes ellipsoideo-cylindricae, 10.8–11.6 µm longae, 4.6–5.8 µm latae, flagellis 2 circa aequilongis praeditae. Papilla bimamillata, ex apice cellulari subexcentrica. Aetate cellulae maiores, ellipsoideae, papillas flagellaque amittentes. Cellulae maturae in cultura liquida crescentes usque ad 13.1 µm longae, 10.8 µm latae; in agaro, in statu palmelloideo usque 18.0 µm longae, 15.4 µm latae attingentes. Chloroplastus viridis, parietalis, cupulatus, incisuris numerosis praeditus, pyrenoide unica centraliter posita, intrusiones irregulares multiplices e membranis thylacoidium compositae eae lamellis amylaceis circumdatae continens. Vacuolae apicales contractiles duae. Stigma ellipsoideum, in parte cellulae 1/3 apicali situm. Zoosporis 2–4–8 per sporangium regenerans. Type:— NEW ZEALAND: Westland: Mt Philistine, 1400 m, preserved cultured specimen from sample collected 30 November 2007, CHR610484. Young cells ellipsoidal–cylindrical, 10.8–11.6 µm long, 4.6–5.8 µm wide, with 2 flagella of approximately equal length. Papilla bimammillate, slightly offset from cell apex. Cells becoming larger and ellipsoidal with age, with papilla and flagella being lost (Fig. 1A). Mature cells in liquid culture up to 13.1 µm long, 10.8 µm wide; on agar, up to 18.0 µm long, 15.4 µm wide in palmelloid state (Fig. 1B). Chloroplast green, parietal, cup-shaped with numerous incisions, with single, centrally located pyrenoid, containing multiple irregular intrusions of thylakoid membranes and surrounded by starch plates (Fig. 1C, D). Two apical contractile vacuoles. Stigma ellipsoidal, located in apical third of cell (Fig. 1A). Reproduction by zoospores, 2–4–8(–16) per sporangium (Fig. 1B). DNA sequence data for both 18 S (Fig. 7) and rbc L (Fig. 9) show that C. palmellomoewusii belongs to the Moewusii clade. Habitat:— Alpine herbfield soil. Distribution:— New Zealand. Etymology:— Reflecting the strong resemblance of the species to the related C. moewusii Gerloff 1940, and occurring predominantly in a palmelloid form in culture. Observations:— Data for the rbc L gene (Fig. 9) show that this species is quite distinct from C. pseudogloeogama, previously found on Mt Philistine (Novis et al. 2008); the two are not sister species. They also differ in size, length:width ratio, papilla structure, and in the more cylindrical shape of the young cells in C. palmellomoewusii. In this respect, and in papilla structure, cell size, and pyrenoid location, the new species strongly resembles C. moewusii; in the absence of molecular data, the specimens would likely have been assigned to this species. Morphology of the pyrenoid is characteristic of the Moewusii clade. According to traditional classification, this strain would belong in section Chlamydella (Ettl 1983), due to its single lateral pyrenoid in a cup-shaped chloroplast. Within this section it is closest to C. gymnogama (Deason 1967), which also has numerous incisions in the chloroplast. C. gymnogama is regarded as a synonym of Lobochlamys segnis (Pröschold et al. 2001), which 18S sequences show to differ from C. palmellomoewusii. Cultures:— LCR-CG1, LCR-CG4.Published as part of Novis, Phil M. & Visnovsky, Gabriel, 2012, Novel alpine algae from New Zealand: Chlorophyta, pp. 1-30 in Phytotaxa 39 on page 14, DOI: 10.11646/phytotaxa.39.1.1, http://zenodo.org/record/489468
Variochloris pyrenoglobularis Novis & Visnovsky 2012, gen. et sp. nov.
No full textVariochloris pyrenoglobularis Novis & Visnovsky, gen. et sp. nov. (Figs 5A–F) Cellulae quoad formam variabiles, sphaericae vel ellipsoideae vel pyriformes, 6.2–9.2 µm longae, 4.6–8.5 µm latae, cytoplasmate granulari, unicae vel in greges aggregatae. Chlorplastus viridis, cupulatus, parietalis, pyrenoidibus 1–2 per thylacoides valde circulariter flexuosae penetratis, quaeque pyrenoglobulis circumdatae. Autosporis 2–4 per sporangium regenerans. Variochloris sita per analysem per sequentias geneticas rbcL in clado valde confirmato conjunctim speciebus alpinis Stichococci novae-zelandiae, Raphidonemtae nivali ex USA atque speciebus duabus “Chlorellae”. Type:— NEW ZEALAND: Westland: Mt Philistine, 1400 m, preserved cultured specimen from sample collected 30 November 2007, CHR610489. Cells variable in shape, spherical to ellipsoidal to pyriform, 6.2–9.2 µm long, 4.6–8.5 µm wide, with granular cytoplasm, single or in groups (Fig. 5A, B). Chloroplast green, cup-shaped, parietal, with 1–2 pyrenoids, penetrated by looped thylakoids, each surrounded by pyrenoglobuli (Figs 5E, F). Reproduction by autospores, 2–4 per sporangium (Fig. 5C). Analysis based on rbc L gene sequences placed Variochloris in a robustly supported clade with alpine species of Stichococcus from New Zealand, Raphidonema nivale Lagerheim from USA, and two “ Chlorella ” species (Fig. 9). The same phylogenetic position was established with 18 S rDNA sequences according to the Bayesian analysis, but no close matches were found with strains previously sequenced. Habitat:— Alpine herbfield soil. Distribution:— New Zealand. Etymology:— Referring to variable shape and presence of pyrenoglobuli. Observations:— Variochloris shares morphological features with Chlorella, but this generic name has priority in the Chlorellales (with the type species Chlorella vulgaris Beijerinck 1890). Pyrenoglobuli are known from taxa in other clades, such as Trebouxia (Tarhanen et al. 2000), and Heveochlorella hainangensis Zhang et al. (2008) in the Watanabea clade. Thylakoid patterns within the pyrenoid matrix in these taxa are also similar. The species of Stichococcus in the same clade as Variochloris do not have pyrenoids, and ultrastructure of this feature in Chlorella ellipsoidea Gerneck 1907 and C. saccharophila (Krüger) Migula 1907 appears to be unknown. The association of coccoid and non-coccoid species in this clade has been noted previously (Novis et al. 2008), and these new results confirm the polyphyly of trebouxiophycean strains referred to Stichococcus. Cultures:— LCR-CG2.Published as part of Novis, Phil M. & Visnovsky, Gabriel, 2012, Novel alpine algae from New Zealand: Chlorophyta, pp. 1-30 in Phytotaxa 39 on page 22, DOI: 10.11646/phytotaxa.39.1.1, http://zenodo.org/record/489468
Achoma brachiatum Novis & Visnovsky 2012, gen. et sp. nov.
No full textAchoma brachiatum Novis & Visnovsky, gen. et sp. nov. (Figs 4A–C) Cellulae sphaericae vel ellipsoideae, 4.6–7.7 µm longae, 3.5–7.3 µm latae. Paries cellularis nullus. Chloroplastus parietalis, cupulatus, lobis incisurisque instructus, pyrenoide unica in forma reticulata e intrusionibus thylacoidium composita in vagina amylacea segmentata includenti continens. Autosporis 2–4–8 per sporangium regenerans. Zoosporae nudae biflagellatae visae, sed sporangia non visa. Type:— NEW ZEALAND: Westland: Mt Philistine, 1400 m, preserved cultured specimen from sample collected 30 November 2007, CHR610487. Cells single, spherical to ellipsoidal, 4.6–7.7 µm long, 3.5–7.3 µm wide. Cell wall absent (Fig. 4B). Chloroplast parietal, cup-shaped, with lobes and incisions, containing single pyrenoid (Fig. 4A) with reticulate pattern of thylakoid intrusions, surrounded by segmented starch sheath (Figs 4B, C). Reproduction by autospores, 2–4–8 per sporangium. Naked biflagellate zoospores observed, but sporangia not seen. Habitat:— Alpine herbfield soil. Distribution:— New Zealand. Etymology:— “Without a bulwark” (wall), “branched” (referring to shape of intruding thylakoids in pyrenoid matrix). Observations:— Vegetative cells often resemble chlamydomonad cells, with cup-shaped chloroplast and obvious polarity, but lack flagella (Fig. 4A). In this feature the cells resemble Chloronomala cuprecola (Groover & Bold 1969) in the Gloeodendrales (sensu Ettl & Gärtner 1995), but the latter forms palmelloid colonies. Cystomonas (Ettl & Gärtner 1987) also resembles chlamydomonad cells, but retains a papilla, and other chlorococcaleans have different chloroplast and/or cell shapes, The rbc L phylogeny (Fig. 9) rules out its placement in any of the chlamydomonad clades known to date. Pyrenoid structure, with branched/reticulate thylakoids inside the matrix, is reminiscent of the structure found in pyrenoids of Hamakko caudatus Nakada & Nozaki 2009. Although no robust splits separate these two species in our rbc L phylogeny, there is also no indication that the two species are close relatives, and relationships in this part of the tree were not resolved. Attempts to amplify the 18S rDNA gene from Achoma were unsuccessful; this could provide more information on the phylogenetic position of the genus. Cultures:— LCR-CG11.Published as part of Novis, Phil M. & Visnovsky, Gabriel, 2012, Novel alpine algae from New Zealand: Chlorophyta, pp. 1-30 in Phytotaxa 39 on page 20, DOI: 10.11646/phytotaxa.39.1.1, http://zenodo.org/record/489468
FIGURE 4. A–C in Novel alpine algae from New Zealand: Chlorophyta
No full textFIGURE 4. A–C. Achoma brachiatum (photographed from culture LCR-CG11). A. Light micrograph of vegetative cells showing variable shape, prominent pyrenoid (pyr), and incised parietal chloroplast (cp). B, C, Transmission electron micrographs of zoospore showing mitochondria (mito), chloroplast containing starch (st) and prominent pyrenoid (pyr), components of flagellar basal apparatus (fba), absence of cell wall, and branching pattern of thylakoids (tm) in the pyrenoid matrix. D, E Schizochlamydella orbicularis (photographed from culture LCR-CG9). D. Light micrograph of mature cells evenly spaced in mucilage, and newly released autospores (arrow). E. Transmission electron micrograph of cell showing mitochondrion (mito), nucleus (nuc) cell wall (cw), and parietal chloroplast containing starch (st) but no pyrenoid. Scales: 10 µm in D (use for A and D), 1 µm in B, C, and E.Published as part of Novis, Phil M. & Visnovsky, Gabriel, 2012, Novel alpine algae from New Zealand: Chlorophyta, pp. 1-30 in Phytotaxa 39 on page 21, DOI: 10.11646/phytotaxa.39.1.1, http://zenodo.org/record/489468
Pseudococcomyxa simplex Fott 1981
No full text<i>Pseudococcomyxa simplex</i> (Mainx) Fott <p> Reference: Novis <i>et al</i>. 2008: 353–354, Fig 3A–I.</p> <p> <b>Observations:—</b> Previously, only data for <i>rbc</i> L were available for this species from Mt Philistine. The 18S data presented here show that <i>Pseudococcomyxa simplex</i> from Mt Philistine is a close relative of <i>P. simplex</i> UTEX 274 (Fig. 8). Both of these strains belong to a clade that includes species of <i>Coccomyxa</i> as well as <i>Paradoxia</i> spp. This clade is distinct from a second clade comprising other species of <i>Coccomyxa</i>; clearly this genus is polyphyletic and requires revision. Interestingly, the oblique division plane characteristic of <i>Pseudococcomyxa</i> (Broady 1987, Novis <i>et al</i>. 2008) is also evident in <i>Paradoxia multiseta</i> Svirenko 1928 during vegetative division (Hegewald & Reymond 1987). Some species currently regarded as <i>Coccomyxa</i>, such as <i>C. confluens</i> (Kützing) Fott 1974, appear to share this feature (Ettl & Gärtner 1995), although how it is distributed phylogenetically is presently unclear. It may be a morphological feature that can be used to separate the two clades.</p>Published as part of <i>Novis, Phil M. & Visnovsky, Gabriel, 2012, Novel alpine algae from New Zealand: Chlorophyta, pp. 1-30 in Phytotaxa 39</i> on page 25, DOI: 10.11646/phytotaxa.39.1.1, <a href="http://zenodo.org/record/4894684">http://zenodo.org/record/4894684</a>
Ornatissimo Doctrina Et Virtute Viro, Dn. Joanni Rodingo: ... D. Gulielmi, Hassiæ Landgravii, +c. Bibliothecario: + ... Margaritae Transfeldiae, novis Sponsis, Gratulantur Amici
No full textElektronische Reproduktion von: Ornatissimo Doctrina Et Virtute Viro, Dn. Joanni Rodingo: ... D. Gulielmi, Hassiæ Landgravii, +c. Bibliothecario: + ... Margaritae Transfeldiae, novis Sponsis, Gratulantur Amici. - Marpurgi : typis Pauli Egenolphi, 1588. - 1 gefalteter Bogen. -
Standort: Universität Marburg, Universitätsbibliothek. -
Signatur: VIII B 331 db, 20 . -
Bemerkungen: Holzschnitteinrahmung. -
(Hassiaca)
Digitalisiert 202
Hubungan antara Tahap Penglibatan Guru Novis di Negeri Melaka dalam Pembelajaran Tidak Formal Melalui Media Sosial dengan Pengurusan Bilik Darjah dan Pengurusan Emosi
No full textKajian tinjauan ini bertujuan untuk mengenal pasti hubungan antara tahap penglibatan guru novis dalam pembelajaran tidak formal melalui media sosial dengan tahap pengurusan bilik darjah dan tahap pengurusan emosi guru novis. Kajian ini dijalankan di 100 buah sekolah rendah dan sekolah menengah daripada tiga buah daerah di negeri Melaka iaitu daerah Alor Gajah, Melaka Tengah dan Jasin. Seramai 219 orang guru novis dari 100 buah sekolah rendah dan sekolah menengah di negeri Melaka telah dipilih sebagai responden bagi kajian ini. Kajian ini merupakan kajian kuantitatif dengan menggunakan kaedah tinjauan. Data kajian dikumpulkan menggunakan satu set soal selidik yang ditadbir secara atas talian menggunakan perisian ‘Google Form’. Instrumen soal selidik dalam kajian ini mengandungi empat bahagian yang mana Bahagian A mengandungi maklumat berkaitan demografi, bahagian B berkaitan tahap penglibatan guru novis dalam pembelajaran tidak formal melalui media sosial, bahagian C berkaitan pengurusan bilik darjah dan akhir sekali Bahagian D meliputi soalan-soalan berkaitan pengurusan emosi guru novis berdasarkan Model Kecerdasan Emosi Bar-On. Hasil analisis kajian menunjukkan bahawa tahap penglibatan guru novis dalam pembelajaran tidak formal melalui media sosial berada pada tahap yang tinggi. Begitu juga dengan tahap pengurusan bilik darjah dan tahap pengurusan emosi guru novis. Dapatan kajian juga menunjukkan bahawa terdapat hubungan yang signifikan antara tahap penglibatan guru novis dalam pembelajaran tidak formal melalui media sosial dengan tahap pengurusan bilik darjah dan pengurusan emosi guru novis. Selain itu, kajian ini turut membincangkan implikasi pembelajaran tidak formal melalui penggunaan media sosial terhadap tahap profesionalisme guru novis serta langkah-langkah yang sesuai bagi meningkatkan keberkesanan pembelajaran tidak formal melalui media sosial dalam kalangan guru novis
FIGURE 4 in Novel alpine algae from New Zealand: Cyanobacteria
No full textFIGURE 4. Godleya alpina Novis & Visnovsky (photographed from cultures LCR-CYTOL and LCR-CY2). A–M light micrographs, N transmission electron micrograph, A. Typical tapered, curved, isopolar trichomes; heterocytes are lacking in 10% BG-11 medium. B. False branching sometimes results in more than two emergent branches (arrows). C. Tolypothrix-like false branching (arrow indicates base of false branch). D. Scytonematoid false branching, especially common in older cultures. E. Culturing on distilled water agar results in development of intercalary heterocytes (h) with two thickened poles. F. Adjacent intercalary heterocytes occur prior to trichome division (arrow). G. Trichomes as a whole are seldom straight around the tapered central region. H. Trichome ends are usually straight and untapered. I. Loop formation (arrow) precedes scytonematoid-type branching. J–M. Hormogone development is strictly isopolar. N. Cells in longitudinal section showing sheath (s) and peripheral thylakoid membranes (tm), which are widened. Scales: 50 µm in B (use for A–M), 1 µm in N.Published as part of Novis, Phil M. & Visnovsky, Gabriel, 2011, Novel alpine algae from New Zealand: Cyanobacteria, pp. 1-24 in Phytotaxa 22 (1) on page 13, DOI: 10.11646/phytotaxa.22.1.1, http://zenodo.org/record/477668
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Mitomycin C in highly myopic eyes - Author reply
No full textOphthalmology. 2005 Feb;112(2):208-18; discussion 219.
Mitomycin C modulation of corneal wound healing after photorefractive keratectomy in highly myopic eyes.
Gambato C, Ghirlando A, Moretto E, Busato F, Midena E.
SourceRefractive Surgery Service and Antimetabolite Therapy Research Unit, Department of Ophthalmology, University of Padova, Padova, Italy.
Abstract
PURPOSE: To evaluate the role of topical mitomycin C in corneal wound healing (CWH) after photorefractive keratectomy (PRK) in highly myopic eyes.
DESIGN: Prospective, double-masked, randomized clinical trial.
PARTICIPANTS: Seventy-two eyes of 36 patients affected by high (>7 diopters) myopia.
METHODS: In each patient, one eye was randomly assigned to PRK with intraoperative topical 0.02% mitomycin C application, and the fellow eye was treated with a placebo. Postoperatively, mitomycin C-treated eyes received artificial tears (3 times daily, tapered in 3 months), whereas the fellow eye was treated with fluorometholone sodium 2% and artificial tears (3 times daily, tapered in 3 months).
MAIN OUTCOME MEASURES: Uncorrected visual acuity (UCVA) and best-corrected visual acuity (BCVA), contrast sensitivity, manifest refraction, and biomicroscopy. Contrast sensitivity was determined using the Pelli-Robson chart. Corneal confocal microscopy documented CWH.
RESULTS: Mean follow-up was 18 months (range, 12-36). No side effects or toxic effects were documented. At 12-month follow-up examination, UCVAs (logarithm of the minimum angle of resolution) were 0.4+/-0.48 and 0.5+/-0.53 (P = .03) in mitomycin C-treated eyes and corticosteroid-treated eyes, respectively. At 1 year, corneal haze developed in 20% of corticosteroid-treated eyes, versus 0% of mitomycin C-treated eyes. At 12, 24, and 36 months, corneal confocal microscopy showed activated keratocytes and extracellular matrix significantly more evident in untreated eyes (Ps = 0.004, 0.024, and 0.046, respectively).
CONCLUSION: Topical intraoperative application of 0.02% mitomycin C can reduce haze formation in highly myopic eyes undergoing PRK.
Comment in
Ophthalmology. 2006 Feb;113(2):357; author reply 357-8
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