5 research outputs found
Some Toxic Effects of Sio2 NPs on Thyroid Gland Function with Histological Changes and Ovary Function in Female Rats
No full textThe study investigated the effects of Silicon Dioxide Nanoparticles (SiO2 NPs) on female rats\u27 thyroid glands and ovaries. Three groups were randomly selected, and two treatment groups were given different SiO2 NPs (25 mg/kg and 100 mg/kg of body weight). The animals were divided into three experimental groups. The thyroid (T3, T4, and TSH), and ovarian (E2 and progesterone) function hormones were measured. The histological study was studied. It was found that significant decrease in T3 levels in the rats group treated with SiO2 NPs doses over 10 days as compared with the control group. The significant decrease in T4 levels was also found in the treated rats groups (10, 20, and 30 days) as compared with the control group. However, a significant elevation in TSH was seen in treated groups as compared with the control group. The current study showed that exposure to SiO2 NPs caused thyroid tissue alteration, with certain follicles filled with colloidal scalloping activity. When exposed to 100 mg/kg of SiO2 NPs for 10 days, more scalloping formation was observed. When treated with 25 mg/kg of SiO2 NPs for 20 days, few follicles filled with colloidal were observed. When treated with 100 mg/kg of SiO2 NPs for 20 days, empty follicles with necrotic cells were observed. In conclusion, SiO2NPs produced structural, functional, and ovarian effects at different concentrations. In the thyroid gland, they decreased T3 and T4 hormone levels and increased TSH levels. In the ovary, they caused structural, functional, and decreased E2 and progesterone levels.
 
Investigation of the Zinc Oxide Nanoparticles Effect on Thyroid and Testosterone Hormones in Male Rats
No full textExposure to zinc oxide nanoparticles (ZnO NPs) has been increasing steadily, causing more attention being paid to their potential toxicity, including cytotoxicity and genotoxicity. Hence, this study aimed to investigate the effect of ZnO NPs on thyroid hormone triiodothyronine (T3), thyroxine (T4), and thyroid-stimulating hormone (TSH) as well as testosterone hormone in male adult rats. A total of 54 Sprague-Dawley albino adult male rats were divided into nine groups each of six rats, daily treated intraperitoneal with ZnO NPs two different doses (30 and 60) mg/kg in three different periods of time (7, 14, and 28) days, as following: Control groups (Groups 1, 2, and 3): Respectively received intraperitoneal injection with distilled water for 7, 14, and 28 days, experimental groups (Groups 4, 5, and 6): They were rats, respectively, received intraperitoneal dose (60 mg/kg) of ZnO NPs for (7, 14, and 28) days, and group (7, 8, and 9) experimental groups were rats, respectively, received intraperitoneal dose (30 mg/kg) of ZnO NPs for (7, 14, and 28) days. Data showed high significant decrease (P < 0.01) in level of T3, T4, TSH, and level of testosterone also decrease at high and low dose for 7, 14, and 28 days
Hypothyroidism and AMH in Iraqi Patients with Chronic Kidney Disease
No full text اجريت الدراسة الحالية للتحري في وضائف الغدة الدرقية و هرمون المضاد لمولر من خلال تقييم مستوياتهم في مرضى الفشل الكلى المزمنة. تمت الدراسة على 50 مريض تم تشخيص اصابتهم بمرض الفشل الكلوي المزمن المرحلة الخامسة اعمارهم بين 20 الى 50 عام (25 ذكور و 25 اناث) و 20 شخص كمجموعة سيطرة تراوحت اعمارهم بين 20-48 عام (10 ذكور و 10 اناث) ، الذين حظروا الى مركز أمراض الكلى وزراعة الأعضاء في مدينة الطب ببغداد في العراق من أبريل 2018 إلى يوليو 2018. اظهرت الدراسة الحالية وجود زيادة غيرمعنوية في مستويات هرمون الانتي ميوليرين في المرضى مقارنة مع مجموعة السيطرة. بينما لوحظ زيادة معنوية عالية في مستويات الهرمون المحفز للغدة الدرقية مقارنة مع مجموعة السيطرة. بينما وجد انخفاض معنوي عالي في مستويات هرمون ثالث يود الثيرونين وهرمون الثيروكسين في مرضى الفشل الكلوي المزمن مقارنة مع مجموعة السيطرة.This study was conducted to investigate thyroid function and Anti-Müllerian hormone (AMH) in (Chronic kidney disease) CKD patients by evaluating their levels in CKD patients, 50 patients were diagnosed to have CKD stage-5, their ages ranged between 20-50 years (25 males and 25 females) who attended the Nephrology and Transplant Center in Medical City of Baghdad- Iraq, they were recruited from April 2018 to July 2018 and were enrolled into the study. The control group consisted of 20 healthy individuals, their ages ranged between 20-48 years (10 males and 10 females). The study showed non-significant (p>0.05) increase in AMH level in CKD patients compared to the control group. On the other hand, TSH was recorded a highly significant (P<0.01) increase in CKD patients in comparison with control. While T3 and T4 levels recorded highly significant (P<0.01) decrease in CKD patients in comparison with control.
Effect of ZnO NPs on Ovarian Histological Structure and Function in Adult Female Rats
No full text تعد تقنية النانو واحدة من أهم التقنيات المستخدمة على نطاق واسع في العديد من المجالات من خلال تغيير خصائص المواد الفيزيائية والكيميائية لتكوين مواد جديدة تسمى الجسيمات النانوية لجعلها أكثر فعالية. تعتبرجسيمات أوكسيد الزنك النانوية هي واحدة من هذه الجزيئات التي تستخدم في تطبيقات متعددة ، خاصة في مستحضرات التجميل ومنتجات الوقاية من اشعة الشمس. ولهذا فإن استخدام هذه الجسيمات بكميات كبيرة يجعلها بتماس دائم مع الجسم والتي من الممكن أن تدخل الدورة الدموية بطرق مختلفة. كان الهدف من هذه الدراسة هو التحري عن تأثير جسيمات أوكسيد الزنك النانوي على التركيب النسجي والوظيفي للمبايض وكذلك تقييم مستوى هرمونات الغدد التناسلية (FSH و LH). ولاجل ذلك تم استخدام 54 من إناث الجرذان البالغة وقسمت بشكل عشوائي إلى ثلاث مجاميع رئيسية وفقًا الى فترات التجريع (1 ، 2 ، 4) أسابيع ، بعدها قسمت كل مجموعة رئيسية إلى ثلاث مجاميع فرعية ، كل مجموعة منها تتكون من 6 جرذان ، احدى هذه المجاميع تم استخدامها كمجموعة سيطرة وحقنت بـ 1 مل من الماء المقطر ، بينما المجموعتين الأخرى استخدمت كمجاميع معالجة وحقنت بـ 1 مل من محلول اوكسيد الزنك النانوي (ZnO NPs) بتركيزين مختلفين منخفض وعالي (50 و 200) مغم / كغم على التوالي ، بمعدل ثلاث جرعات في الأسبوع عن طريق الحقن داخل الصفاق. أظهرت النتائج تغيرات نسجية مختلفة في مقاطع أنسجة المبايض لدى الجرذان المجرعة بتراكيز وفترات زمنية مختلفة ، وكذلك أظهرت النتائج ايضا" انخفاض معنوي (p≤ 0.01) في مستويات هرمون الاستروجين والبروجسترون و LH و FSH ، لدى الحيوانات المعالجة بتراكيز وفترات زمنية مختلفة. وقد تم الأستنتاج بأن لجزيئات دقائق الزنك النانوية القدرة على إتلاف أنسجة المبيض ، وتعطيل وظائفه الخلوية مما أدى الى خلل في مستويات الهرمونات التناسلية (P و E2 و LH و FSH).Nanotechnology is one of the most important techniques that is widely used in many fields by changing the physical and chemical properties of materials to form new scale substances called nanoparticles to make them more effective. ZnO NPs is one of these particles that is used in multiple applications, especially in cosmetic and sunscreen products. Thus, the uses of these particles in great quantities make them in constant contact with the body and can enter the circulating blood in a different ways. The aim of this study was to find out the effect of ZnO NPs on the histological structure and functions of the ovary, as well as to determine the levels of gonadotropin hormones (FSH and LH). Therefore, 54 adult female rats were randomly divided into three primary groups according to the duration of exposure (1, 2, and 4 weeks). These groups were then further divided into three subgroups, each of which consisted of six rats, one of which served as a control group and was injected with 1 ml of distilled water, while the others served as treated groups and were injected with 1 ml of ZnO NPs at low and high concentrations 50 and 200 mg/kg respectively, an average of three intra-peritoneal injections per week. The results showed different histological changes in the ovarian tissue sections of treatment rats at different doses and durations, as well as significant decrease (p≤ 0.01) in the levels of estrogen, progesterone, LH, and FSH hormones in treated animals at different doses and durations. It can be concluded that the ZnO NPs have ability to damage the ovarian tissue, and disruption of its cellular functions that caused an imbalance in reproductive hormones levels (P and E2, LH and FSH)
EFFECTS OF SILVER NANOPARTICLES ON THYROID GLAND STRUCTURE AND FUNCTION IN FEMALE RATS
No full textObjective: Due to their unique properties, silver nanoparticles (AgNPs) gained a broad utilization in nano-based industries and medicine, which may expose human to increased levels of NPs. However, little is known about their potential harmful effects on endocrine physiology. Hence, this study aimed to investigate the potential dose- and time-dependent outcomes of AgNPs on serum levels of thyroid hormones and thyroid gland histology in female rats.Methods: A total of 60 female rats were divided into three groups (each of 20 animals), treated with AgNPs for (10, 20, and 30) days. Within each treatment period, animals were assigned into four subgroups each of five rats; control treated with vehicle and the others treated with 12.5, 25, and 50 mg/kg of AgNPs, respectively, by intraperitoneal injection. At the end of treatments, all rats were sacrificed; blood samples were obtained and analyzed for serum levels of T3, T4, and thyroid-stimulating hormone (TSH). Thyroid gland was removed and weighed then kept in buffered formalin solution for microscopic examination.Results: The data showed a significant increase in the weight of the thyroid gland after 20 and 30 days of the treatment with 50 mg/kg of AgNPs, while the 25 mg/kg dose of AgNps resulted in significant increase only after 30 days. Serum levels of T3 and TSH were nonsignificantly altered by AgNPs in all the treatment groups. Thyroxin levels (T4) were significantly decreased after long-term exposure. Histological specimens of AgNPs treated group showed disturbance of the normal architecture of the thyroid tissue with degeneration of thyroid follicles and desquamated luminal cells.Conclusion: The results of the current study suggested the possible disrupting potential of long-term exposure to high level of AgNPs on thyroid gland function and histology in female rats
