87,767 research outputs found
Assessing wildfire radiative power and pollution in the Amazon Basin
No full textA Master of Science project submitted to the Graduate Program at the Institute of Physics, University of Sao Paulo, on behalf of Mr Thiago F Nobrega
Complete genome sequence of the Escherichia coli phage Ayreon
No full textWe report the whole-genome sequence of a new Escherichia coli temperate phage, Ayreon, comprising a linear double-stranded DNA (dsDNA) genome of 44,708 bp
Rational identification of a colorectal cancer targeting peptide through phage display
No full textColorectal cancer is frequently diagnosed at an advanced stage due to the absence of early clinical indicators. Hence, the identification of new targeting molecules is crucial for an early detection and development of targeted therapies. This study aimed to identify and characterize novel peptides specific for the colorectal cancer cell line RKO using a phage-displayed peptide library. After four rounds of selection plus a negative step with normal colorectal cells, CCD-841-CoN, there was an obvious phage enrichment that specifically bound to RKO cells. Cell-based enzyme-linked immunosorbent assay (ELISA) was performed to assess the most specific peptides leading to the selection of the peptide sequence CPKSNNGVC. Through fluorescence microscopy and cytometry, the synthetic peptide RKOpep was shown to specifically bind to RKO cells, as well as to other human colorectal cancer cells including Caco-2, HCT 116 and HCT-15, but not to the normal non-cancer cells. Moreover, it was shown that RKOpep specifically targeted human colorectal cancer cell tissues. A bioinformatics analysis suggested that the RKOpep targets the monocarboxylate transporter 1, which has been implicated in colorectal cancer progression and prognosis, proven through gene knockdown approaches and shown by immunocytochemistry co-localization studies. The peptide herein identified can be a potential candidate for targeted therapies for colorectal cancer.Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2019 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte and the Project FCOMP-01–0124-FEDER-021053 (PTDC/SAU-BMA/121028/2010). Débora Ferreira is recipient of a fellowship supported by a doctoral advanced training (call NORTE-69-2015-15) funded by the European Social Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. Franklin L. Nobrega, Sara Granja and Ligia R Rodrigues acknowledge FCT for the grants SFRH/BD/86462/2012, SFRH/BPD/117858/2016 and SFRH/BSAB/142991/2018, respectively. Catarina Barbosa-Matos also acknowledge her research grant UMINHO/BI/395/2018info:eu-repo/semantics/publishedVersio
Análise de canais, fluxos e protagonismos: um estudo de caso sobre a comunicação interna na Embrapa caprinos e ovinos.
No full textResumo: O artigo traça uma avaliação de projeto de Comunicação Interna na Embrapa Caprinos e Ovinos, observando o impacto das mudanças em canais, veículos e processos de comunicação, por meio da pesquisa junto ao público interno da entidade. Como destaque, os resultados de satisfação e sugestões dos colaboradores, analisados sob a ótica de correntes teóricas da Comunicação Organizacional, para verificar o cumprimento de premissas de uma comunicação interna participativa e de diálogo, prevista como objetivo do projeto
Complete genome sequences of two T4-like Escherichia coli bacteriophages
No full textBacteriophages and their proteins have potential applications in biotechnology for the detection and control of bacterial diseases. Here, we describe the sequencing and genome annotations of two strictly virulent Escherichia coli bacteriophages that may be explored for biocontrol strategies and to expand the understanding of phage-host interactions.BN/Stan Brouns La
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Exploiting Bacteriophage Proteomes: The Hidden Biotechnological Potential
No full textBacteriophages encode many distinct proteins for the successful infection of a bacterial host. Each protein plays a specific role in the phage replication cycle, from host recognition, through takeover of the host machinery, and up to cell lysis for progeny release. As the roles of these proteins are being revealed, more biotechnological applications can be anticipated. Phage-encoded proteins are now being explored for the control, detection, and typing of bacteria; as vehicles for drug delivery; and for vaccine development. In this review, we discuss how engineering approaches can be used to improve the natural properties of these proteins and set forth the most innovative applications that demonstrate the unlimited biotechnological potential held by phage-encoded proteins.Accepted Author ManuscriptBN/Stan Brouns La
The whole of cyber defense : syncing practice and theory
No full textThe paper is drawn in part from and extends the dissertation of the lead author: de Nobrega, K. (2023). Cyber defensive capacity and capability: A perspective from the financial sector of a small state. [Doctoral Thesis, Tilburg University]. CentER, Center for Economic Research.This review explores the problems Chief Information Security Officers (CISOs) and other cyber
professionals face when defending their organization against cyberattacks. Using a Cyber Defense
Grid, which was developed based on military science and cybersecurity concepts and terminology,
we coded 125 articles published in Information Systems (IS) journals. We also employed
three avenues (i.e., lenses) from cybernetic theory to frame the coding results to derive cyber
defense strategies. In particular, we propose three strategic cyber defense modes: reactive, heuristic,
and proactive. Taken together, these three modes suggest ways in which organizations can
react strategically within the whole cyber defense domain.http://www.elsevier.com/locate/jsisam2024InformaticsSDG-09: Industry, innovation and infrastructureSDG-16:Peace,justice and strong institution
Prophages are associated with extensive, tolerated CRISPR-Cas auto-immunity
No full textCRISPR–Cas systems require discriminating self from non-self DNA during adaptation and interference. Yet, multiple cases have been reported of bacteria containing self-targeting spacers (STS), i.e. CRISPR spacers targeting protospacers on the same genome. STS has been suggested to reflect potential auto-immunity as an unwanted side effect of CRISPR–Cas defense, or a regulatory mechanism for gene expression. Here we investigated the incidence, distribution, and evasion of STS in over 100 000 bacterial genomes. We found STS in all CRISPR–Cas types and in one fifth of all CRISPR-carrying bacteria. Notably, up to 40% of I-B and I-F CRISPR–Cas systems contained STS. We observed that STS-containing genomes almost always carry a prophage and that STS map to prophage regions in more than half of the cases. Despite carrying STS, genetic deterioration of CRISPR–Cas systems appears to be rare, suggesting a level of escape from the potentially deleterious effects of STS by other mechanisms such as anti-CRISPR proteins and CRISPR target mutations. We propose a scenario where it is common to acquire an STS against a prophage, and this may trigger more extensive STS buildup by primed spacer acquisition in type I systems, without detrimental autoimmunity effects as mechanisms of auto-immunity evasion create tolerance to STS-targeted prophages
[Newspaper Clipping: Author Claims Evidence of Second JFK Assassin #1]
No full textNewspaper article titled "Author Claims Evidence of Second JFK Assassin." The article states that author Richard J. Whalen concluded "that there is circumstantial evidence to support the theory of a second assassin in the shooting of President John F. Kennedy.
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