1,721,198 research outputs found
Sensitivity Comparison of an Organic Based DNA Extraction Method to a Silica Based DNA Extraction System Utilizing Carrier RNA and Effects of a Post-PCR Purification Process
Full text linkNguyen, Chau D., Sensitivity Comparison of an Organic Based DNA Extraction Method to a Silica Based DNA Extraction System Utilizing Carrier RNA and Effects of a Post-PCR Purification Process. Master of Science (Forensic Genetics), December 2008, 2008, 84 pp., 24 tables, 9 figures, References, 33 titles. Organic extraction from forensic samples has consistently produced high DNA yields. However, organic extraction is time-consuming and contains many steps where sample manipulation can occur. In this study, sensitivity of organic extraction method is compared to QIAamp DNA Investigator Kit, a silica based extraction utilizing carrier RNA. The results suggested that the QIAamp DNA Investigator Kit is just as sensitive as organic extraction method in obtaining partial and full DNA profiles. The QIAamp DNA Investigator Kit is less time-consuming than organic extraction and can be automated on the QIAcube. The study also studied the reliability of a post-PCR purification kit in obtaining a better DNA profile. The Qiagen MinElute PCR Purification Kit increased the number of loci detected in 65% of samples tested
Waste Heat Dissipation and Effluent Water Temperatures from Black Dog Lake
No full textNorthern States Power Company's Black Dog Power Generating Plant discharges
its condenser cooling water into a pond called Black Dog Lake, which
releases it through two outlets into the Minnesota River. In this study
it was found that an average 63 percent of the heat load rejected by the plant
was dissipated to the atmosphere by the lake during a period from March
1974 through January 1975. Daily heat dissipation varied from 15 to 128 percent
because of temporary heat storage in the lake (lag) under transient
weather conditions. Daily lake effluent temperature fluctuations were determined
and compared to Minnesota River temperature fluctuations. The lake
very effectively reduces the impact of thermal discharges on the Minnesota
River. Its effectiveness was found to vary with season, with a maximum in
midsummer and a minimum in midwinter, presumably due to sinking plumes. A
mathematical model of the unsteady well-mixed type predicts summer effluent
water temperatures well, but is not applicable for winter conditions.Northern States Power CompanyStefan, Heinz; Nguyen, Chau. (1976). Waste Heat Dissipation and Effluent Water Temperatures from Black Dog Lake. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/116951
Mixing and Heat Transfer of Cooling Water Discharges from the Monticello Nuclear Power Generating Plant into the Mississippi River
No full textWater temperature data obtained in the Mississippi River downstream
from the Monticello Nuclear Power Generating Plant during 32 field surveys
have been analyzed and reduced to a form which permits the prediction of
certain thermal plume characteristics using readily available plant
operational, hydrologic and meteorological input data. To achieve this
goal, dimensionless parameters are derived by analysis and applied in order
to reduce the field data to simple graphical form and in some cases to
semi-empirical equations. All results indicate that lateral turbulent
mixing is the main mechanism by which cooling water effluents from the
Monticello plant are diluted over the first three miles downstream from the
outlet. There is no significant horizontal stratification and not much
heat transfer to the atmosphere within the river reach studied.Northern States Power CompanyStefan, Heinz; Lake, Gary; Nguyen, Chau. (1976). Mixing and Heat Transfer of Cooling Water Discharges from the Monticello Nuclear Power Generating Plant into the Mississippi River. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/116946
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
A Novel -72 (T→A) β-Promoter Mutation Causing Slightly Elevated HbA2 in a Vietnamese Heterozygote.
No full textWe report a novel β+-thalassemia mutation found in a Vietnamese family. The molecular defect T→A lies at -72 of the β-globin gene promoter, within the conserved CCAAT box. The index case was a 5-year-old child having red blood cells indices close to normal and slightly increased level of HbA2 (3.96%). The expression of the mutated β allele was inferred by luciferase reporter assay in K562 cells. The β -72 determinant is the eighth β-thalassemic mutation identified in Vietnam and it was not previously reported in any population. The absence of homozygous or compound heterozygous states did not allow us to precisely predict either its clinical impact or its relevance in management programs. Our results further underline the importance of identifying and characterizing new or rare β+-thalassemic alleles in carrier screening and prenatal diagnosis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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