305 research outputs found
The Impact of Interaction Between Author and Audience Shown on the Example of Hideaki Anno's "Neon Genesis Evangelion"
No full textDie vorliegende Arbeit beschäftigt sich mit Hideaki Anno als Person, seinem Werk und wie das Fandom seines Werkes dieses schließlich direkt beeinflusst hat. Dazu wird folgende Forschungsfrage gestellt: Wie genau hat das Fandom das Werk Annos verändert? Diese wird Anhand einer Analyse der Metaebene durch die Verfasserin untersucht. Dabei dient die Serie Neon Genesis Evangelion (1995-1996) als Ausgangspunkt und die Neuauflage der Serie, die sogenannten Rebuild-Filme (2007-2020), als Vergleichsobjekt. Die Metaebene beschreibt dabei, wie sich ein Medium über die reale Welt und seinen eigenen Einfluss darauf äußer
The discovery of SycO reveals a new function for type three secretion effector chaperones
No full textThe Type Three Secretion (T3S) system is a device used by many Gram-negative pathogens that allows bacteria to deliver effector proteins straight into the eukaryotic cell cytosol. These effectors interfere with various signaling pathways to subvert the host cell functions. The secretion machinery of the T3S system consist of a basal body spanning the bacterial inner and outer membrane followed by a stiff hollow needle outside the bacterium. The fully assembled secretion apparatus constitute a continuous hollow conduit that connects the bacteria to the eukaryotic target cell. After cell contact, virulence proteins -called effectors- are injected directly into the cytosol of the host cell via the T3S apparatus. Several effectors of the T3S system require the assistance of specific cytosolic chaperones to be efficiently exported. There are three classes of T3S chaperones. Effector proteins are assisted by Class I chaperones. Although Class I chaperones are well characterized, their main function is still a matter of controversy. In this thesis, we demonstrate that orf155 encodes a specific chaperone for the effector YopO that we called SycO. We showed that SycO enhances YopO secretion in vitro and is required for translocation of YopO into infected cells. By pulldown assay we demonstrated that residues 20 to 77 of YopO are required and sufficient for SycO binding. Using crosslinking experiments and size exclusion chromatography analysis, we determined the stoichiometry of purified SycO and YopO-SycO complexes. SycO alone forms dimers in solution and the YopO-SycO complex has a 1:2 stoichiometry. These results suggested that SycO is a typical chaperone of the Class I. YopO is a serine/theronine kinase that interacts with Rho and Rac and disrupts the cytoskeleton of the target cells. YopO has been shown to localize at the cell plasma-membrane. By transfection of YopO-EGFP hybrid proteins into HEK293T cells, we demonstrated that the chaperone-binding domain (CBD) coincides with the membrane localization domain of YopO. Nevertheless, the CBD was not needed for the kinase activity of YopO. By ultracentrifugation, we also showed that the CBD causes YopO aggregation in the bacteria, when SycO does not cover it. Further, we show that the CBD of YopE and YopT also caused aggregation in the bacteria in the absence of SycE and SycT respectively. YopE, YopT and T3S effectors in other systems also act at the membrane of the eukaryotic host cell. We propose a new hypothesis concerning the role of T3S chaperones. The sub-cellular localization domain of effectors is aggregation-prone and creates the need for a chaperone inside bacteria. We propose that masking such aggregation-prone localization domains may be a general function for type III effector chaperones
ON DESIGN OF RESISTIVE WAVE METER
No full textAlthough the resistive wave meter, which measures the electric resistance bet-ween two electrodes, may be regarded old fashioned, it is used frequently with suc-cess for many laboratory experiments. Morever, it is used fruitfully for measure-ment of the internal waves which are generated at the interface of fresh and saltwaters. This is the reason why the author describes the method of designing this typeof wave meter. The fundamental principle of design is led by analyzing the measur-ing circuit. Pratical examples of such designing by the author are shown herewith.Although the resistive wave meter, which measures the electric resistance bet-ween two electrodes, may be regarded old fashioned, it is used frequently with suc-cess for many laboratory experiments. Morever, it is used fruitfully for measure-ment of the internal waves which are generated at the interface of fresh and saltwaters. This is the reason why the author describes the method of designing this typeof wave meter. The fundamental principle of design is led by analyzing the measur-ing circuit. Pratical examples of such designing by the author are shown herewith
A simulation study of the growth and the spatial distribution of plankton in the estuary of the Yodo River, Osaka Bay, Japan
No full text
Precise sinusoidal signal extraction from noisy waveform in vibration calibration
No full textPrecise extraction of sinusoidal vibration parameters is essential for the
dynamic calibration of vibration sensors, such as accelerometers. However,
several standard methods have not yet been optimized for large background
noise. In this work, signal processing methods to extract small vibration
signals from noisy data in the case of accelerometer calibration is discussed.
The results show that spectral leakage degrades calibration accuracy. Three
methods based on the use of a filter, window function, and numerical
differentiation are investigated with theoretical calculations, simulations,
and experiments. These methods can effectively reduce the contribution of the
calibration system noise. The uncertainty of micro vibration calibration in the
National Metrology Institute of Japan is reduced by two orders of magnitudes
using the proposed methods. The theoretical analyses in this work can lay the
foundation for the optimization of signal processing in vibration calibration,
and can be applied to other dynamic calibration fields
A new method on recycling coefficient measurement using impurity pellet injection in a large helical device
No full text胎盤由来間葉系幹細胞の胎盤機能検査法としての役割に関する研究
No full textThe cellular and molecular mechanisms responsible for pregnancy-related disorders remain unclear. We investigated the feasibility of using placenta-derived mesenchymal stem cells (MSCs) as a tool to study such pregnancy-related disorders. We isolated and expanded adequate numbers of cells with characteristic features of MSCs from the chorionic plate (CP-MSCs), chorionic villi (CV-MSCs), and decidua basalis (DB-MSCs) of human term placental tissues. All placenta-derived MSCs expressed pregnancy-associated C14MC microRNA (miRNA) (miR-323-3p). Interestingly, the placenta-specific C19MC
miRNAs (miR-518b and miR517a) were clearly expressed in CP-MSCs and CV-MSCs of foetal origin, but were barely expressed in DB-MSCs of maternal origin. Furthermore, expression levels of placenta-specific C19MC miRNAs in CV-MSCs remained stable during the ex vivo expansion process and across different pregnancy phases (first trimester versus third trimester). High-efficiency siRNA transfection was confirmed in twice-passaged CV-MSCs with
little toxicity, and microarray analysis was used to screen for miR-518b target genes. Placenta-derived MSCs, especially CV-MSCs, are a potential tool for investigating the role of placental miRNAs in pregnancy-related disorders.長崎大学学位論文 学位記番号:博(医歯薬)甲第1006号 学位授与年月日:平成30年3月20日Author: Naoki Fuchi, Kiyonori Miura, Hanako Doi, Tao-Sheng Li & Hideaki MasuzakiCitation: Scientific Reports, 7, 46220; 201
Development of Japanese Language Learning Content Using Immersive Virtual Reality
No full textIn recent years, remarkable progress has been made in immersive virtual reality (iVR). However, iVR in Japanese language education (JLE) lags behind education for other languages. In this paper, the author reviews iVR Japanese language learning content that is currently under development. Further, the author argues that in order to collaborate with experts in different fields during the development process, it is necessary to accumulate knowledge in those fields and to consider how to reconstruct such knowledge.
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