1,721,048 research outputs found
Differential subcellular expression of P525LFUS as a putative biomarker for ALS phenoconversion
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La comunicazione non verbale come strumento di inclusione e integrazione. Risultati di un questionario
Full text linkL’immigrazione rappresenta una sfida d’integrazione a livello locale ed internazionale e per questo negli ultimi anni sono aumentati gli interventi volti a facilitare il processo di integrazione dei cittadini di Paesi Terzi. Un’attenzione particolare viene posta a questioni di carattere linguistico, culturale e sociale. In generale, manca invece la riflessione sull’importanza del linguaggio non-verbale nella comunicazione interculturale e di come questo aggiunga una chiave di interpretazione cruciale al messaggio che viene veicolato: la mancata attenzione al non-verbale può tradursi in episodi di esclusione sociale e di stereotipizzazione che possono avere conseguenze determinanti per l’inserimento dell’individuo nella società. Si ritiene invece che una maggiore consapevolezza delle differenze nel linguaggio non-verbale esistenti tra le culture potrebbe favorire la comprensione e l’accettazione delle diversità, migliorare le relazioni personali e professionali con i migranti e favorirne l’inserimento nella società. Questo articolo presenta i risultati di un questionario creato e distribuito nell’ambito del Programma Nazionale del Fondo Asilo, Migrazione e Integrazione (FAMI) e volto a comprendere il grado di consapevolezza sul non verbale di operatori sociali a contatto con migranti provenienti da differenti realtà geografiche, sociali e religiose e a raccogliere dati utili a sviluppare un progetto che includa le dinamiche del non verbale nella formazione degli operatori che lavorano nell’ambito dell’integrazione e dell’inclusione dei migranti
A Deletion of the Nuclear Localization Signal Domain in the Fus Protein Induces Stable Post-stress Cytoplasmic Inclusions in SH-SY5Y Cells
Full text linkMutations in Fused-in-Sarcoma (FUS) gene involving the nuclear localization signal (NLS) domain lead to juvenile-onset Amyotrophic Lateral Sclerosis (ALS). The mutant protein mislocalizes to the cytoplasm, incorporating it into Stress Granules (SG). Whether SGs are the first step to the formation of stable FUS-containing aggregates is still unclear. In this work, we used acute and chronic stress paradigms to study the SG dynamics in a human SH-SY5Y neuroblastoma cell line carrying a deletion of the NLS domain of the FUS protein (homozygous: ΔNLS–/–; heterozygous: ΔNLS+/–). Wild-type (WT) cells served as controls. We evaluated the subcellular localization of the mutant protein through immunoblot and immunofluorescence, in basal conditions and after acute stress and chronic stress with sodium arsenite (NaAsO2). Cells were monitored for up to 24 h after rescue. FUS was expressed in both nucleus and cytoplasm in the ΔNLS+/– cells, whereas it was primarily cytoplasmic in the ΔNLS–/–. Acute NaAsO2 exposure induced SGs: at rescue,>90% of ΔNLS cells showed abundant FUS-containing if compared to less than 5% of the WT cells. The proportion of FUS-positive SGs remained 15–20% at 24 h in mutant cells. Cycloheximide did not abolish the long-lasting SGs in mutant cells. Chronic exposure to NaAsO2 did not induce significant SGs formation. A wealth of research has demonstrated that ALS-associated FUS mutations at the C-terminus facilitate the incorporation of the mutant protein into SGs. We have shown here that mutant FUS-containing SGs tend to fail to dissolve after stress, facilitating a liquid-to-solid phase transition. The FUS-containing inclusions seen in the dying motor neurons might therefore directly derive from SGs. This might represent an attractive target for future innovative therapies
Detailed field mapping and revised litho-biostratigraphy of the Castelvetere Formation cropping out at the NW Campania-Basilicata border (Southern Apennines, Southern Italy)
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Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
REASSESSMENT OF THE AGE OF BASEMENT IN THE VAVILOV BASIN DRILLED AT ODP SITE 651 (TYRRHENIAN SEA, MEDITERRANEAN)
Full text linkWe carried out a biostratigraphic study based on calcareous nannofossils in sediments from Ocean Drilling Program (ODP) Site 651, drilled in the basalt-floored Vavilov sub-basin of the Tyrrhenian Sea (western Mediterranean) during Leg 107. The expedition achieved its primary objective to collect basement samples to date the ocean crust and obtain new data for reconstructing the geological evolution of the area. Studies on the ODP Site 651 succession determined the nature of the basement and provided time constraints for tectonic and magmatic processes in the basin, but contradictory lines of evidence emerged for the age of the basalts based on radiometric ages, paleomagnetic signature and planktonic foraminifera data. In an attempt to clarify the basement age, we performed detailed sampling for calcareous nannofossil analysis to update the biostratigraphy and chronology of the sediments overlying the basement. We determined the sediment accumulation rate using reliable nannofossil biohorizons that have a robust chronology, which increases up-section from ~ 2 cm/kyr to 3.4 cm /kyr. The age of the basalt/sediment contact was obtained through extrapolation of the sedimentation rate from the deepest datable portion of the sediments down to the top of basement. The inferred age for the top of the basalt is at least 4.1-4.2 Ma and differs from previous evaluations. This age for the basalt in the Vavilov Basin basement is consistent to a reversed interval C2Ar within the Gilbert magnetochron, supporting one of the chronologies previously proposed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
New geological map at a 1:10.000 scale and tectono-stratigraphic review of the NW Campania-Basilicata boundary (Southern Apennines, Southern Italy)
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