486 research outputs found
Analysis of Water-Soluble Proteins by Two-Dimensional Electrophoresis in the Encystment Process of Colpoda cucullus Nag-1 and Cytoskeletal Dynamics
No full textSogame, Yoichiro, Kojima, Katsuhiko, Takeshita, Toshikazu, Kikuchi, Shiho, Shimada, Yuto, Nakamura, Rikiya, Arikawa, Mikihiko, Miyata, Seiji, Kinoshita, Eiji, Suizu, Futoshi, Matsuoka, Tatsuomi (2020): Analysis of Water-Soluble Proteins by Two-Dimensional Electrophoresis in the Encystment Process of Colpoda cucullus Nag-1 and Cytoskeletal Dynamics. Acta Protozoologica 59: 107-120, DOI: 10.4467/16890027AP.20.009.13264, URL: http://dx.doi.org/10.4467/16890027ap.20.009.1326
The efficacy of second-line tyrosine kinase inhibitor for patients with metastatic non-clear cell renal cell carcinoma following first-line immune-oncology combination therapy
No full textCitation: Kazutoshi Fujita, Yuto Matsushita, Shingo Toyoda, Takahiro Kojima, Shimpei Yamashita, Hisanori Taniguchi, Keisuke Monji, Ryo Ishiyama, Shuichi Tatarano, Kimihiko Masui, Eijiro Nakamura, Tomoyuki Kaneko, Goshi Kitano, Takanobu Motoshima, Satoru Kira, Takaya Murashima, Hiroaki Hara, Naotaka Nishiyama, Hideaki Miyake, Hiroshi Kitamura, Hirotsugu Uemura, The efficacy of second-line tyrosine kinase inhibitor for patients with metastatic non-clear cell renal cell carcinoma following first-line immune-oncology combination therapy, World Journal of Urology, 42(1), 2024-09-26, https://doi.org/10.1007/s00345-024-05252-
<Posters>Indices of smooth Calabi-Yau varieties
No full text於 京都大学理学研究科セミナーハウス (2024年10月22日-10月25日)2024年度科学研究費補助金 基盤研究(A)(課題番号 20H00111, 代表 小木曽啓示)2024年度科学研究費補助金 基盤研究(A)(課題番号 21H04429, 代表 並河良典)Date : October 22nd - 25th, 2024Location: Kyoto University (North Campus), Science Seminar HouseJSPS KAKENHI Grant-in-Aid (A) 20H00111 (Keiji Oguiso)JSPS KAKENHI Grant-in-Aid (A) 21H04429 (Yoshinori Namikawa)Organizers: Yohsuke Matsuzawa, Yusuke Nakamura, Kazuhiko Yamak
Overexpression of the translocon accessory protein YajC alleviates toxicity of the endogenous pore-forming toxin LdrA in Escherichia coli
No full textFig. 7 in Analysis of Water-Soluble Proteins by Two-Dimensional Electrophoresis in the Encystment Process of Colpoda cucullus Nag-1 and Cytoskeletal Dynamics
No full textFig. 7. Effects of 10 µM taxol (A) and 10 µM cytochalasin B (B) on Ca2+/overpopulation-mediated globulation of C. cucullus Nag-1 (A-1, B-1) and ciliary resorption (A-2, B-2). A-1, B-1 − The rate of encysting (rounded) cells was expressed as a percentage of the total number of tested cells (100 randomly selected cells). Open squares (negative control). The cells were suspended in 1 mM Tris-HCl (pH 7.2) solu- tion without inhibitors at low cell density ( 30,000 cells/ml) (Ca2+/overpopulation stimulation). In this condition, the encystment was markedly induced. Open circles (experiment). The cells were suspended in an encystment-inducing medium containing taxol (Ta) or cytochalasin B (CB) at high cell density (> 30,000 cells/ml). Points and attached bars correspond to the means of 5 measurements (100 cells per measurement) obtained from different batches and standard errors, respectively. A-2, B-2 − Length of cilia at 2 h after onset of encystment induction in the presence or absence of taxol (Ta) or cytochalasin B (CB). In the negative control [Induced without 'Ta' (0 h) or Induced without 'CB' (0 h)], the cultured cells were collected, then suspended in encystment-inducing medium, and quickly fixed with 3.7% paraformaldehyde. In the positive control [Induced without 'Ta' (2 h) or Induced without 'CB' (2 h)], the cells were suspended for 2 h in an encystment-inducing medium without inhibitors at high cell density (> 30,000 cells/ml), and then fixed with 3.7% paraformaldehyde. In the experimental groups [Induced with 'Ta' (2 h) or Induced with 'CB' (2 h)], the cells were suspended for 2 h in an encystment-inducing medium containing inhibitors at high cell density (> 30,000 cells/ml), and then fixed with 3.7% paraformaldehyde. Columns and attached bars correspond to the means in 26 cells and standard errors, respectively.Published as part of Sogame, Yoichiro, Kojima, Katsuhiko, Takeshita, Toshikazu, Kikuchi, Shiho, Shimada, Yuto, Nakamura, Rikiya, Arikawa, Mikihiko, Miyata, Seiji, Kinoshita, Eiji, Suizu, Futoshi & Matsuoka, Tatsuomi, 2020, Analysis of Water-Soluble Proteins by Two-Dimensional Electrophoresis in the Encystment Process of Colpoda cucullus Nag-1 and Cytoskeletal Dynamics, pp. 107-120 in Acta Protozoologica 59 on page 117, DOI: 10.4467/16890027AP.20.009.13264, http://zenodo.org/record/835695
Serotonin receptor 4 (5-hydroxytryptamine receptor Type 4) regulates expression of estrogen receptor beta and cell migration in hormone-naive prostate cancer
No full textBackground: Estrogens are considered to potentially play some roles in the development and progression of prostate cancer through estrogen receptor beta (ERβ). However, additional factors which could influence the clinical outcome of the patients through modulating these steroid signalings have also been proposed. Among these, increased expression of serotonin receptor especially that of 5-hydroxytryptamine receptor Type 4 (5-HTR4) has been recently proposed to be involved in autocrine/paracrine mechanisms of castration-resistant prostate cancer, but the presence and clinical significance of 5-HTR4 in hormone-naive prostate cancer (HNPC) and its interaction with hormonal signaling pathways have remained virtually unknown. Materials and Methods: We evaluated the status of 5-HTR4 in 112 human HNPC cases (acinar adenocarcinoma) using immunohistochemistry and correlated the findings with clinicopathological features of individual patients and the status of androgen receptor (AR) and ERβ. To further elucidate its underlying mechanisms, androgen-dependent human prostate carcinoma cell line, LNCaP, expressing 5-HTR4, was treated by 5-HTR4 agonist. Results: 5-HTR4 immunoreactivity was detected in 34% of prostate cancer cases examined (38/112) and was significantly correlated with the status of ERβ but not with that of AR and other clinicopathological factors of the patients. Results of in vitro studies demonstrated that 24 h incubation with 5-HTR4 agonist (10 nM) increased the expression level of ERβ messenger RNA compared to controls. 5-HTR4 agonist (100 nM) significantly inhibited LNCaP carcinoma cell migration (P < 0.05). Conclusion: Results of our present study indicated that 5-HTR4 signaling upregulated ERβ expression in HNPCs and could impact on biological processes in HNPC
Generalized dual expression for set relations by means of sublinear scalarization functions (Study on Nonlinear Analysis and Convex Analysis)
No full textIn the literature, characterization for set relations are of various application to optimality conditions of set optimization problems, variational principles for set-valued maps, theorems of the alternative, certain robustness of vector optimization problems, and so on. In this paper, the author presents properties of scalarization functions as dual expression of set relations. Comparing to existing results, one can confirm their uniqueness in their relaxed conditions. Also, we show our results suggest some robust positive semi-definite optimization problems
ネズミマラリア原虫Plasmodium yoeliiのErythrocyte Binding-Likeタンパク質は赤血球侵入過程における不可逆的結合形成に重要である
No full textPlasmodium malaria parasites multiply within erythrocytes and possess a repertoire of proteins whose function is to recognize and invade these vertebrate host cells. One such protein involved in erythrocyte invasion is the micronemal protein, Erythrocyte Binding-Like (EBL), which has been studied as a potential target of vaccine development in Plasmodium vivax (PvDBP) and Plasmodium falciparum (EBA-175). In the rodent malaria parasite model Plasmodium yoelii, specific substitutions in the EBL regions responsible for intracellular trafficking (17XL parasite line) or receptor recognition (17X1.1pp. parasite line), paradoxically increase invasion ability and virulence rather than abolish EBL function. Attempts to disrupt the ebl gene locus in the 17XL and 17XNL lines were unsuccessful, suggesting EBL essentiality. To understand the mechanisms behind these potentially conflicting outcomes, we generated 17XL-based transfectants in which ebl expression is suppressed with anhydrotetracycline (ATc) and investigated merozoite behavior during erythrocyte invasion. In the absence of ATc, EBL was secreted to the merozoite surface, whereas following ATc administration parasitemia was negligible in vivo. Merozoites lacking EBL were unable to invade erythrocytes in vitro, indicating that EBL has a critical role for erythrocyte invasion. Quantitative time-lapse imaging revealed that with ATc administration a significant number of merozoites were detached from the erythrocyte after the erythrocyte deformation event and no echinocytosis was observed, indicating that EBL is required for merozoites to establish an irreversible connection with erythrocytes during invasion.長崎大学学位論文 学位記番号:博(医歯薬)甲第1093号 学位授与年月日:平成30年9月5日Author: Yuto Kegawa, Masahito Asada, Takahiro Ishizaki, Kazuhide Yahata, Osamu KanekoCitation: Parasitology International, 67(6), pp.706-714; 201
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