4,620 research outputs found
Advances in Engineering and Application of Optogenetic Indicators for Neuroscience
No full textOur ability to investigate the brain is limited by available technologies that can record biological processes in vivo with suitable spatiotemporal resolution. Advances in optogenetics now enable optical recording and perturbation of central physiological processes within the intact brains of model organisms. By monitoring key signaling molecules noninvasively, we can better appreciate how information is processed and integrated within intact circuits. In this review, we describe recent efforts engineering genetically-encoded fluorescence indicators to monitor neuronal activity. We summarize recent advances of sensors for calcium, potassium, voltage, and select neurotransmitters, focusing on their molecular design, properties, and current limitations. We also highlight impressive applications of these sensors in neuroscience research. We adopt the view that advances in sensor engineering will yield enduring insights on systems neuroscience. Neuroscientists are eager to adopt suitable tools for imaging neural activity in vivo, making this a golden age for engineering optogenetic indicators. Keywords: optogenetic tools; neuroscience; calcium sensor; voltage sensor; neurotransmitter
[David Mitchell and Descendants]
No full textCopy of "David Mitchell and Descendants" originally written by Thomas Mitchell, Jr. The manuscript starts with David Mitchell, possibly a Revolutionary soldier, and details what is known about his life and those of his descendants. The record ends with the marriage of his widow, Sarah Patterson Mitchell Frear, to Abraham Frear. It states that she was the mother and grandmother of many Frears and Mitchells in the family. After the record, there is a note about the original manuscript's author; it was then copied by Ora Osterhout
In the Garden, Danielle Mitchell, Spring 2020
No full textDanielle Mitchell is a rising senior from Compton, California majoring in anthropology and sociology. She is a gifted writer who conducted very special interviews in SIS Seminar
Hall Street, Danielle Mitchell, Spring 2020
No full textDanielle Mitchell is a rising senior from Compton, California majoring in anthropology and sociology. She is a gifted writer who conducted very special interviews in SIS Seminar
Peer Interview Script, Danielle Mitchell, Spring 2020
No full textDanielle Mitchell is a rising senior from Compton, California majoring in anthropology and sociology. She is a gifted writer who conducted very special interviews in SIS Seminar
Excerpts of Interviews with Peers, Danielle Mitchell, Spring 2020
No full textDanielle Mitchell is a rising senior from Compton, California majoring in anthropology and sociology. She is a gifted writer who conducted very special interviews in SIS Seminar
David Mitchell on Cloud Atlas
No full textAuthor David Mitchell joined LMU via Skype to discuss his novel, Cloud Atlas
Local Church Week Pastor Andre Mitchell
No full textPastor Andre Mitchell, Pastor, Author, CEO, Deliverance Temple/Andre Mitchell Ministries, Muncie, IN, speaks on how connecting with other believers is important to your faith for Local Church Week
Letter from Carl Hayden to Bishop Walter Mitchell
No full textLetter from Carl Hayden to Walter Mitchell on building a Union Chapel in the Grand Canyon
W. F. Mitchell
No full textEarlier this year, an article was published in the News Bulletin
(February 2012) on the background of Captain Boyns Hedley
Hocking, a dentist who became one of the first casualties in the
bombing of Darwin in 1942. The author, W F Mitchell, has kindly
provided a summary of the 70th anniversary activities held in
Darwin in February 2012 to commemorate this significant event in
the Northern Territory?s historyDate:2012-09News Bulletin no. 413, p. 36 - 37
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