39 research outputs found
Intermediate Filaments in Breast Cancer Progression, and Potential Biomarker for Cancer Therapy: A Narrative Review
No full textWidad Aghnia Shalannandia,1 Yoan Chou,2 Muhammad Hasan Bashari,1,3 Astrid Feinisa Khairani1,3 1Graduate School of Master Program in Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Sumedang, Jatinangor, West Java, Indonesia; 2Graduate School of Master Program in Anti Aging and Aesthetic Medicine, Faculty of Medicine, Universitas Padjadjaran, Sumedang, Jatinangor, West Java, Indonesia; 3Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Sumedang, Jatinangor, West Java, IndonesiaCorrespondence: Astrid Feinisa Khairani, Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Jalan Raya Bandung – Sumedang Km 21, Jatinangor, Sumedang, West Java, 45363, Indonesia, Tel +62-22-7795594, Email [email protected]: Intermediate filaments are one of the three components of the cytoskeletons, along with actin and microtubules. The intermediate filaments consist of extensive variations of structurally related proteins with specific expression patterns in cell types. The expression pattern alteration of intermediate filaments is frequently correlated with cancer progression, specifically with the epithelial-to-mesenchymal transition process closely related to increasing cellular migration and invasion. This review will discuss the involvement of cytoplasmic intermediate filaments, specifically vimentin, nestin, and cytokeratin (CK5/CK6, CK7, CK8/CK18, CK17, CK19, CK20, CSK1), in breast cancer progression and as prognostic or diagnostic biomarkers. The potential for drug development targeting intermediate filaments in cancer will be reviewed. Keywords: breast malignancy, cytokeratin, EMT, intermediate filament, keratin, nestin, vimenti
Myeloid Cell Leukemia-1 (MCL-1) and Its Correlation with the Prognostic Scoring System in Chronic Myeloid Leukemia
No full textThe prognostic scoring system is used to assess the prognosis of Chronic Myeloid Leukemia (CML) patients to get the right treatment strategy. Poor prognosis, treatment resistance, and tumorigenesis have been associated with the overexpression of Myeloid Cell Leukemia-1 (MCL-1), an anti-apoptotic protein in several hematologic malignancies, including CML. Research on the correlation between MCL-1 levels and the scoring system prognostic in patients with CML is still limited. This study aims to evaluate the correlation between MCL-1 levels and the prognostic scoring system in chronic phase CML patients. This research was conducted on chronic phase CML patients who came to the Division of Hematology and Medical Oncology, Department of Internal Medicine, Dr. Hasan Sadikin Hospital Bandung, Indonesia. Data is collected from medical records of patients examined for MCL-1 protein levels from previous studies and results of prognostic scoring systems (Sokal, Hasford, and EUTOS. Data were analyzed to evaluate the correlation between the MCL-1 level and the prognostic scoring system. Forty patients had a male-female ratio of 1.5. The average age is 40±11 years, with an age range of 19 to 61 years. The median MCL-1 protein level was 0.27 (min 0.02-max 4.1). Statistical analysis showed no significant correlation between MCL-1 levels and Sokal, Hasford, and EUTOS scores in chronic phase CML patients (p=0.285; p=0.923 and p=0.663, respectively)
Rationality of Empirical Antibiotic Usage among Digestive Surgery Inpatients at Dr. Hasan Sadikin General Hospital, Bandung, Indonesia: Gyssens Criteria Analysis
No full textBackground: Many empirical antibiotics are prescribed unnecessarily, contributing to the rise in the incidence of antibiotic resistance. Preventing infection of surgical sites is one of the most frequent purposes of empirical antibiotic usage. Therefore, this study aimed to analyze the rationality of empirical antibiotic usage among digestive surgery inpatients based on the Gyssens criteria and clinical features outcomes.Methods: A descriptive method was used with a cross-sectional design. Data was collected from medical records of patients underwent digestive surgery and had received empirical antibiotic therapy post-surgery from July to September 2021 at Dr. Hasan Sadikin General Hospital, Bandung, Indonesia. Subsequently, the data was analyzed using the Gyssens method to qualitatively assess the rationality of antibiotic use based on specific criteria, classified from rational (category 0) to various levels of irrational use (categories I-VI). Clinical outcomes were evaluated by assessing leukocyte count, clinical symptoms of fever, and the appearance of surgical site infection before and after administration of empirical antibiotics.Results: In total, 70 empirical antibiotic prescriptions were obtained from 42 patients, with only 40% of patients using rational antibiotics, whereas 60% of antibiotics were used inappropriately (category I-VI). Clinical evaluation showed no fever, edema, or erythema and reduced leukocytosis and exudate/pus post-therapy.Conclusions: Inappropriate empirical antibiotic prescription for digestive surgery inpatients at Dr. Hasan Sadikin General Hospital based on the Gyssens criteria is high. Nonetheless, clinical outcomes showed improvements in infection-related parameters. These results emphasize the importance of stricter adherence to antibiotic stewardship and regular evaluation of empirical antibiotic practices to optimize usage and patient outcomes
Soyghurt Potentially Controls the Level of sFlt1 and PLGF in Preeclampsia Maternal Serum-Induced Placental Trophoblast Cell in vitro
No full textAstrid Feinisa Khairani,1 Uci Ary Lantika,2 Julia Ramadhanti,1 Muhammad Hasan Bashari,1,3 Widad Aghnia Shalannandia,1 Tenny Putri Wikayani,4 Achadiyani Achadiyani,1 Mulyanusa Amarullah Ritonga5 1Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Jatinangor, West Java, Indonesia; 2Department of Biomedical Sciences, Faculty of Medicine, Universitas Islam Bandung, Bandung, West Java, Indonesia; 3Research Centre of Oncology and Stem Cell, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, Indonesia; 4Culture and Cytogenetic Laboratory, Universitas Padjadjaran, Bandung, West Java, Indonesia; 5Department of Obstetrics and Gynecology, Hasan Sadikin General Hospital, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, IndonesiaCorrespondence: Astrid Feinisa Khairani, Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Jalan Raya Bandung – Sumedang Km 21, Jatinangor, West Java, 45363, Indonesia, Tel +62-22-779559445363, Email [email protected]: To observe the effect of soya yoghurt (Soyghurt), which is high in flavonoid substance, on the expression of preeclampsia biomarkers (sFLT-1 and PLGF) on preeclampsia serum-induced trophoblast primary cell culture isolated from placental tissue.Methods: The trophoblast primary culture was induced by preeclampsia serum (10%). The Soyghurt treatment was performed with 2.5%, 5%, and 7.5% Soyghurt supernatant concentrations in culture media. The expression of preeclampsia markers, sFLT-1 and PLGF, were evaluated using ELISA.Results: Expression of sFLT-1 on preeclampsia-induced cell culture treated with Soyghurt was significantly lowered compared to the untreated group (p 0.05).Conclusion: This study demonstrates the potential effect of Soyghurt’s in balancing preeclampsia marker expression by inhibiting the expression of sFLT-1 in preeclampsia serum -induced trophoblast cells.Keywords: preeclampsia, PLGF, sFLT-1, soya yoghurt, trophoblas
Aaptamine Inhibits Lipid Accumulation and Pparg and Slc2a4 Expression While Maintaining the Methylation of the Pparg Promoter During 3T3-L1 Adipocyte Differentiation
No full textEko Fuji Ariyanto,1 Ibnu Wijaya,2 Zaky Alif Pradian,2 Agung Putri Mayasari Bhaskara,2 Mohammad Parezal Lastialno,3 Putri Halleyana Adrikni Rahman,1 Muhammad Hasan Bashari,1 Nandina Oktavia,1 Muhammad Iman Pratama Putra,4 Yuni Susanti Pratiwi,1 Henhen Heryaman,1 Diah Dhianawaty1 1Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Sumedang, Indonesia; 2Undergraduate Program of Medical Doctor, Faculty of Medicine, Universitas Padjadjaran, Sumedang, Indonesia; 3Master Program of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia; 4Department of Internal Medicine, Faculty of Medicine, Universitas Padjadjaran - Hasan Sadikin General Hospitale, Bandung, IndonesiaCorrespondence: Eko Fuji Ariyanto, Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Sumedang, Indonesia, Tel +6281316099791, Email [email protected]: Excessive adipogenesis plays a role in the development of obesity and related metabolic disorders. Aaptamine is an alkaloid compound that has been proven to have various effects, however, no studies have yet investigated its effects on adipogenesis. This study aims to examine whether aaptamine inhibits lipid accumulation and Pparg and Slc2a4, two important genes in adipogenesis, mRNA expression, and increases the methylation of the Pparg promoter. This study strengthens the insights regarding these genes regulation, with future research potentially expanding to other adipogenic regulators for a broader perspective.Methods: The effects of aaptamine (0 μM, 25 μM and 50 μM) were investigated in 3T3-L1 preadipocytes. The adipocytes were differentiated using a medium containing 3-isobutyl-1-methylxanthine, dexamethasone, and insulin. Cell viability was evaluated by the MTT assay, gene expression was analyzed by RT-qPCR, and lipid accumulation was determined using Oil Red O staining. Pyrosequencing was performed to measure the methylation of the Pparg promoter region.Results: Aaptamine treatment significantly dose-dependently decreased lipid accumulation and inhibited Pparg and Slc2a4 mRNA expression. However, there were no significant differences in the methylation level of the Pparg promoter.Conclusion: Aaptamine inhibits lipid accumulation and Pparg and Slc2a4 mRNA expression while maintaining the methylation level of the Pparg promoter during 3T3-L1 adipocyte differentiation.Keywords: aaptamine, adipocyte differentiation, DNA methylation, Pparg, Slc2a
CLINICOHISTOPATHOLOGICAL STUDY OF TESTICULAR TUMOURS IN DR. HASAN SADIKIN GENERAL HOSPITAL BANDUNG
No full textObjective: The purpose of this research was to describe the clinical and histopathological characteristics of testicular tumours at Hasan Sadikin General Hospital (RSHS) between 2017-2021. Material & Methods: This research was a cross-sectional descriptive study examining the clinical and histopathological profiles of testicular tumours. The secondary data sources included medical records from the Department of Urology, Anatomic Pathology, Medical Records, and Hospital Information System (SIRS). The collected data covered age, ethnicity, clinical symptoms, site, stage, annual prevalence rate, and analyzed using Microsoft Excel. Results: There were 42 patients who had primary testicular tumour. The highest prevalence of testicular tumours at RSHS was in 2019 consist of 12(29%) patients and the lowest in 2020 were 6(14%) patients. Most patients were >35 years old amounting 19(45%) patients, Sundanese ethnicity were 29(69%) patients, and testicular enlargement revealed in 27(64%) patients. Majority patients were in stage 0/IB 36(86%) patients and occurred in unilateral testis which 34(80%) patients, with 3(7%) patients metastasized. The most common histopathological type was seminoma 18(43%) cases followed by prepubertal-type yolk sac tumour 9(21%) patients. Seminomas, and the fibroma-thecoma group were mostly in patients over 35 years old, while prepubertal-type yolk sac tumour was more commonly in children under 15 years old. Conclusion: Prevalence of testicular tumours increased from 2017 to 2019, then started to decrease in 2020. Histopathologically, seminoma was the most common type. These tumours were most commonly found in older individuals (>35 YO) and unilateral, diagnosed in stage 0/IB, and were mostly presented with enlargement of testis.
Keywords: Testicular tumours, clinical features, histopatholog
Snakebites and the Effect of Serum Anti Bisa Ular (SABU) Antivenom at Dr. Hasan Sadikin General Hospital Bandung, Indonesia: an Overview Period 2015–2019
No full textBackground: Snakebite is considered a global health issue, especially in the Southeast Asian region. However, data regarding snakebite cases in Indonesia are still very limited. This study aimed to explore the venomous snakebite cases and the serum anti bisa ular (SABU) antivenom effect in treating venomous snakebite caused by other than the snake species indicated in currently available SABU antivenom formulas.Methods: The analytical descriptive method with a cross-sectional study design was conducted in 2021 using a total sampling from the medical records of 63 patients diagnosed with snakebite at Dr. Hasan Sadikin General Hospital Bandung from 2015 to 2019. Characteristics of patients, clinical manifestation, and correlation between snakes species and outcomes after treatment with or without administration of SABU antivenom were collected.Results: Out of 63 patients, males (79%) were predominant with an average age of 39 years. Fifty-six patients arrived at the Emergency Room less than 24 hours after the occurrence (89%). Most cases were categorized as grade 2 (41.%) where the upper extremities were commonly bitten the body area (64%). Edema (83%) was the most common manifestation. There was no correlation between snake antivenom administered to a specific snake species and the stated outcome (p=0.053), meaning that SABU antivenom might be an effective alternative to treat more types of snakebites.Conclusions: Snakebites are most common in males, attack the upper extremities, categorized as grade 2 with edema. Administration of SABU antivenom provides the similar outcome compared to the group caused by the Javan spitting cobra, Banded krait, and Malayan pit viper
The AP-1 transcription factor JunB promotes multiple myeloma cell proliferation, survival and drug resistance in the bone marrow microenvironment
No full textKonsep pendidikan akhlak menurut kitab Adab Ad-Dunya wa Ad-Din karangan Imam Hasan Ali Bin Muhammad Bin Habib Al-Bashari Al-Mawardi
No full textINDONESIA:
Pendidikan Akhlak bertanggungjawab terhadap keseluruhan pembentukan karakter dalam perbaikan jati diri mereka. Pergaulan siswa yang semakin hari semakin parah yang mengarah kepada pergaulan bebas, minum-minuman keras, tidak tau sopan santun dan kenakalan remaja yang merajalela seluruh tatanan moral dan religiusitas tergugat. Pendidikan Akhlak perlu untuk membentengi anak-anak muda dengan nilai-nilai religius ajaran agama Islam. Nilai-nilai religius tidak cukup di ajarkan di kelas yang sifatnya adalah pengetahuan saja tetapi harus diaplikasikan dalam kehidupan.Pendidikan Akhlak adalah sebuah pemberdayaan potensi akal manusia agar tercipta prilaku yang baik dalam rangka mencapai kebahagiaan yang paripurna. Manusia merupakan makhluk yang multi dimensial. Bukan saja karena manusia secara teologis mempunyai rasa dan kesadaran untuk mengembangkan pola kehidupannya akan tetapi lebih dari itu sekaligus juga menjadi objek dalam keseluruhan aktivitas dan kreatifitasnya. Dalam melakukan penelitian ini, peneliti menggunakan metode penelitian kepustakaan . Sedangkan metode yang digunakan untuk mengumpulkan data adalah melalui karangan Syaikh Abu Hasan yang didokumentasikan pada karya agung beliau yakni kitab adab Ad-Dunya wa Ad-Din. Untuk menganalisis data, peneliti menggunakan tekhnik analisis deduktif kualitatif deduktif, yaitu mendeskripsikan data-data yang ada untuk menggambarkan realitas yang dijelaskan pada kitab karangan beliau sesuai dengan fenomena yang sebenarnya.
Al-Mawardi adalah seorang tokoh pemikir Islam yang hidup pada masakejayaan peradaban Islam. Ini dibuktikan dengan karya beliau yaitu kitab Adab Ad-Dunya Wa Addin. Dalam kitab tersebut dijelaskan tentang konsep dan pandangan beliau tentang Akhlak.Menurut Al-Mawardi, manusia mempunyai dua potensi dasar yaitu akal danhawa. Akal membawa kecenderungan manusia untuk berbuat baik sedangkan hawamemiliki kecenderungan membawa manusia untuk berprilaku buruk. potensi akalmanusia dapat mengontrol kecenderungan untuk berprilaku buruk, ketika potensi akalmanusia diberdayakan melalui bimbingan seorang guru. Untuk itu pendidikan harus dilakukan dalam kerangka melatih pola kerjaakal secara terus menerus dalam merespon lingkungan. Selain itu, proses pendidikan ini harusdilakukan dalam upaya bagaimana pendidikan memberikan kebebasan kepada anakdidik untuk menjadi mandiri dan menjadi dirinya sendiri.
ENGLISH:
Moral education responsible for the overall formation of enshriningthe character intheir identity. Student socializing increasingly severed thus leads to the promiscuity, boozing, doing uncivilized manners and juvenile delinquency thus absurdly rampant throughout the moral order and religiousity defendant.Moral education necessed to fortified the youth within the religious of Islamic teachings values. Religious values are unsufficiently objucated in classes thus not the only knowledge nature itself but must be applied livingly. Moral education is an empowering potential of the human mind in order to create good behavior and to achieve complete happiness. The human being are the multi dimensional beings. Not the only reason because human beings have the theological sense and awareness to develop some kind of a life pattern but ultimately thus also becomes an object in the overall activity and creativity. In conducting this study, researchers used the library research method. While the methods used to collect data is through a bouquet of Sheikh Abu Hasan documented in the book of his great work of Ad-Dunya wa Ad-Din.To analyzing the data, researcher used the qualitative deductive analysis technique, thus to describing the livings data to sketching some of the vivid reality on his book bouquet paralelly within the actual phenomenon.
Sheikh Abu Hasan al-Mawardi was a prominent Islamic thinker who live during the heyday of Islamic civilization. This evidenced by his work which thus in the Adab Wa Addin Ad-Dunya. In the book explained about the concept and his views about the Morals upon his viewing. According to Sheikh Abu Hasan al-Mawardi, humans posessed the two basic potential thus reasonable mind and appetency. The reasonable brings the human tendency to do good while the lust having tendency to bring people to evilly behave. the potential of the human mind can control the tendency to behave even more trickingly, when the potential of the human mind is empowered under the guidance of a teacher. For those, the education must be constructed in behalf of the drawing to trains the continous mind patterns of encryption behalf of the response ablity to the environment. In addition, the educational process should be conducted in an effort to study how to speech freedom to the students and encouraged them how to be independent and be themselves
العناصر الداخلية في مسرحية "القصر في الجنة" لعلي أحمد باكثير
No full textPenelitian ini membahas tentang unsur-unsur instrinsik yang terdapat dalam drama “Al-Qashru fii Al-Jannah” yang ditulis oleh Ali Ahmad Bakathir. Penulis mengambil judul ini dikarenakan drama ini mengandung banyak pelajaran hidup yang dapat kita ambil dan dapat kita terapkan dalam keseharian.Dalam penelitian ini penulis menggunakan penelitian dengan metode deskriptif kualitatif dimana hasilnya berupa kata-kata tertulis dari objek yang telah diamati oleh penulis, adapun data yang digunakan dalam penelitian ini berupa teks drama “Al-Qashru Fii Al-Jannah”. Data didapat dari penelitian kepustakaan yang berhubungan atau terkait dengan teori undur-undur instrinsik dan drama “Al-Qashru Fii Al-Jannah”. Setelah peneliti melakukan analisis, peneliti menghasilkan penelitian yang sesesuai dengan rumusan masalah Hasil dari penelitian ini adalah analisis unsur-unsur instrinsik dalam drama “Al-Qashru Fii Al-Jannah” karya Ali Ahmad Bakatsir. Drama ini bertemakan tentang keikhlasan dalam bersedekah dalam kehidupan dimana keihlasan itu akan mendapatkan ganjaran tersendiri dari Allah.swt baik di dunia maupun di akhirat. Tokoh utama dalam drama ini adalah Abu Muhammad Al-Farisy dan Hamzah Aziyat Al-Khurosani sedangkan tokoh pembantu dalam drama ini adalah Al-Hasan Al-Bashari, Asma, Misykadanah, Habibah, Abas, Abdullah, dan Ahmad. Alur dalam drama ini adalah alur maju.Latar tempat dalam drama ini yaitu: rumah Abu Muhammad, Madinah Munawaroh, dan Majlis Tujar. Sedangkan latar waktu yaitu: tadi malam (al-barihah). Gaya bahasa dalam drama ini yaitu: kalam khobari, kalam insya’i dan tibaq. Dalam drama terkandung beberapa amanat seperti tidak boleh tamak dan ridha atas apa yang ditetapkan Allah. Sw
