1,720,956 research outputs found
Development of a real-time PCR for detection of Mycoplasma agalactiae in bulk tank milk samples and epidemiology of infection in Sardinia
No full textDEVELOPMENT OF A REAL-TIME PCR FOR DETECTION OF MYCOPLASMA AGALACTIAE IN BULK TANK
MILK SAMPLES AND EPIDEMIOLOGY OF INFECTION IN SARDINIA
Carta T. [1], Mannu F.[2], Fadda M.[1], Ibba I.[3], Muggianu D.[3], Turrini F.[2], Pittau M.[1], Chessa B.[1]
[1]Dipartimento di Medicina Veterinaria, Università degli Studi di Sassari ~ Sassari ~ Italy, [2]Nurex s.r.l. ~ Sassari ~ Italy, [3]Associazione
Regionale Allevatori Sardegna (ARAS) ~ Oristano ~ Italy
In this work the Mycoplasma agalactiae p48 gene was used as a diagnostic marker for contagious agalactia (CA) of sheep and goats by Real-Time
PCR. The p48 gene encodes an invariable, constantly expressed, immunodominant surface lipoprotein belongs to the basic membrane protein
family. The Real-Time PCR test based on p48 resulted specific and sensible. The test performance were evaluated on bulk tank milk samples
collected from 1064 ovine and 66 goat farms in sardinian region. 4.8% of sheep farms and 4.5 % of goat farms tested positive. Our results showed
that the test based on the p48 gene can be used on bulk tank milk for detection and epidemiological surveillance of Mycoplasma agalactiae
infections
Variabilità del profilo acidico del latte ovino prodotto in Sardegna: potenzialità della spettroscopia FT-MIR.
No full textL’impiego di metodi spettroscopici aspecifici on-line per l’analisi degli alimenti
è in costante crescita. In tale ambito, l’applicazione di metodi spettroscopici nel medio
infrarosso (FT-MIR) abbinati all’analisi multivariata permette di ottenere modelli che
predicono, in maniera pressoché istantanea e con un basso costo per campione ma senza
detrimento per l’accuratezza, la concentrazione degli analiti nella matrice studiata.
Consapevoli del crescente significato che il profilo acidico del latte (o quantomeno le sue
classi costituenti) ha nell’ambito della valorizzazione della materia prima o dei suoi prodotti
di trasformazione, l’obiettivo del presente studio è stato quello di cercare di oggettivarne
la variabilità, su latte ovino prodotto in Sardegna, mediante tecniche spettroscopiche online
FT-MIR. Lo studio è stato effettuato su circa 6000 campioni di latte ovino, prelevati tra il
febbraio ed il giugno 2012 da diversi allevamenti situati nelle province di Oristano, Nuoro
e Cagliari. Per ogni campione è stato acquisito lo spettro FT-MIR tramite uno spettrometro
Milkoscan FT-6000 in dotazione al Laboratorio del latte dell’Associazione Regionale Allevatori
(ARA) della Sardegna. Il tenore di grasso e la concentrazione delle principali classi degli
acidi grassi: saturi (SFA), insaturi (UFA), monoinsaturi (MUFA) e polinsaturi (PUFA) è
stato quindi valutato tramite modelli di predizione basati su regressione multivariata PLS
(Partial Least Square regression) in precedenza messi a punto e validati dal nostro gruppo
di ricerca. I valori ottenuti in predizione sono stati elaborati tramite analisi delle componenti
principali (PCA). I risultati hanno permesso di evidenziare le differenze compositive del
latte ovino dovute a variabilità sia geografica che stagionale. Di rilievo è la separazione che
si osserva tra i campioni di latte di fine inverno e di fine primavera, e quella che si nota
tra i campioni provenienti da allevamenti situati in montagna ed allevamenti situati in
pianura.Variability of fatty acids profile in Sardinian sheep milk by FT-MIR spectroscopic
techniques. - The use of on-line aspecific spectroscopic methods in food analysis has
been constantly increasing. Mid-InfraRed (MIR) spectroscopic methods, combined with
multivariate analysis, has been used to build models that predict the concentration of an
analyte in a food matrix with good accuracy, in real time and at low cost. Been aware of the
growing importance of the fatty acid profile (at least in the categories of fatty acids) in the
enhancement of milk and its products, the aim of this work was to make objective the fat
variability of Sardinian sheep milk by using FT-MIR spectroscopic techniques. The study
was conducted on around 6000 Sardinian sheep milk samples collected between February
and June 2012 in different farms of the areas of Oristano, Nuoro and Cagliari. The FT-MIR
spectrum of each sample was acquired by using the spectrometer MilkoScan FT-6000 (FOSS)
used in the Milk Laboratory of the Regional Breeding Association (ARA Sardegna). Total
fat and the classes of Saturated Fatty Acids (SFA), Unsaturated Fatty Acids (UFA), Mono-
Unsaturated Fatty Acids (MUFA), and Poli-Unsaturated Fatty Acids (PUFA), were estimated
by using Partial Least Squares (PLS) models previously built and validated by our research
group. The predicted values were analyzed by the Principal Component Analysis (PCA) which
has allowed to evidence differences between samples in relation to geographical or seasonal
variability. The greatest separation has been noted between the samples of late winter and
early summer and between samples gathered, in the same period, in farms that differ for
altitude (high altitude, defined as “mountain”, versus low altitude, defined as “plane”
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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