1,721,067 research outputs found
Isolation and characterization of thermophilic bacteria from the hot springs in Malaysia / Siti Aisyah Mualif
Full text linkThermophiles can be defined as organisms capable of growing at high temperatures (60°C to 80°C) as compared to the mesophiles (37°C). These organisms can be isolated in hot springs and most studies aim for their thermostable enzymes. Water samples from six hot springs (Selayang, Setapak, Klah, Labis , Gadek , and Pedas) were collected using the LaMotte bottom sampling dredge. Samples were mixed with CTYE medium and another portion without CTYE medium. These samples were transported in cold boxes and in the laboratory were incubated at 60°C for 1-2 days. Reference strain Thermus aquaticus (ATCC®25104™) was included as control. After 24 h incubation, turbid samples (indicating bacterial growth) were plated and incubated in the same condition using CTYE supplemented with 3% agar. Pure colonies were chosen from each culture plate for identification by (i) conventional biochemical methods and (ii) molecular characterization. Another portion of the water samples were processed directly for DNA. Extracted DNA were used to amplify 16S rDNA gene by PCR method. The amplicons were sent for sequencing and results obtained were aligned with representative 16S rDNA sequences of related taxa using CLUSTAL W 1.83 software. Based on 16S rDNA study, five new strains of bacteria (Klah, Klah 2, Labis, Gadek, and Pedas) were isolated and confirmed to be closely related to Geobacillus spp. These strains were earlier identified by conventional tests and found to be Gram-positive bacilli, motile, oxidase and catalase positive. More isolates were identified from the same locations: four Gram negative bacilli (Sly, Sly 2, Klah 1, and Gadek 1) and three Gram-positive spore-forming bacilli (Sly 1, Klah 3, Gadek 2). Out of the four Gram-negatives, two strains (Gadek I and Klah 1) were found to be closel y related to Thermus strains. Strains Klah 1, Klah 2, Klah 3, Labis , Gadek , Gadek 2 and Pedas were found to be protease enzyme producers. All the Gram-positives were related to thermophilic Bacillus spp. Most of the organisms from the hot springs can be maintained in the laboratory using CTYE medium at 60°C. The hot springs water temperature was in the range of 48°C to 95°C and pH 6.8 to 7.9. Morphological examination, conventional biochemical tests and physiological studies were found to be useful in the preliminary identification of new isolates. However, 16S rDNA sequences and phylogenetic analysis has proven to be more discriminative tool for accurate determination of unknown organism. In conclusion, this study therefore provides evidence that the hot springs in Malaysia supports the growth of both Thermus and Bacillus spp. These bacteria may have numerous potentials as useful enzyme producers for biotechnological applications
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Pengaruh Penggunaan Smartphone Terhadap Kecerdasan Emosional Siswa Kelas IV MI Islamiyah Kedung Jambu Paron Ngawi
No full textMualif, Nursan. 2020. Pengaruh Penggunaan Smartphone Terhadap Kecerdasan Emosional Siswa Kelas IV MI Islamiah Kedung Jambu, Skripsi, Fakultas Tarbiyah dan Ilmu Keguruan, Jurusan Pendidikan Guru Madrasah Ibtidaiyah. Pembimbing Edi Irawan, M.Pd.
Kata kunci: Smartphone, Kecerdasan Emosional.
Smartphone pada masa kini menjadi kebutuhan yang umum bagi masyarakat. Dengan terus-menerus bermain smartphone, anak akan sibuk sendiri tanpa memikirkan lingkungan di sekitarnya. Anak perlu dibatasi dalam penggunaan smartphone. Jangan sampai anak punya ketergantungan dengan smartphone sejak kecil. Mengasah kecerdasan emosional sejak kecil bisa menumbuhkan kompetensi sosial pada anak. Salah satunya dengan menumbuhkan rasa empati terhadap anak.
Tujuan penelitian ini adalah: 1) Untuk mengetahui penggunaan smartphone siswa di MI Islamiyah Kedung Jambu Paron Ngawi. 2) Untuk mengetahui kecerdasaan emosional siswa di MI Islamiyah Kedung Jambu Paron Ngawi. 3) Untuk mengetahui pengaruh antara penggunaan smartphone terhadap kecerdasan emosional siswa di MI Islamiyah Kedung Jambu Paron Ngawi.
Penelitian ini menggunakan metode kuantitatif. Jenis penelitian yang digunakan peneliti adalah causalitas comparative atau hubungan sebab akibat. Pendekatan dasar kausal komparatif melibatkan kegiatan peneliti yang diawali dari mengidentifikasi pengaruh variabel mengidentifikasi pengaruh variabel satu terhadap variabel lainnya, kemudian mencari kemungkinan variabel penyebabnya. Dalam penelitian ini karena jumlah populasi kurang dari 30 maka populasi dijadikan sampel semua dengan menggunakan sampling jenuh. Sampel yang diambil adalah 24 siswa kelas IV. Teknik pengumpulan data menggunakan angket penggunaan smartphone dan kecerdasan emosional. Teknik analisis data menggunakan uji SPSS.
Berdasarkan analisis data ditemukan bahwa 1) secara umum penggunaan smartphone siswa ada tiga kriteria, yakni: a) pengguna berat sebanyak 41,7% siswa. b) pengguna sedang sebanyak 50% siswa. c) pengguna ringan sebanyak 8,3% siswa. 2) secara umum kecerdasan emosional sebanyak 25% siswa menunjukkan kriteria tinggi. Sebanyak 58,3% siswa menunjukkan kriteria sedang. Dan sebanyak 16,6% siswa menunjukkan kriteria rendah. Dengan demikian tingkat kecerdasan emosional siswa MI Islamiyah Kedung Jambu adalah rata-rata sedang. 3) terdapat pengaruh penggunaan smartphone terhadap kecerdasan emosional siswa kelas IV MI Islamiyah Kedung Jambu. Tingkat signifikansi sebesar 0,000 < 0,05 maka menunjukan bahwa ada pengaruh antar penggunaan smartphone terhadap kecerdasan emosional siswa kelas IV MI Islamiyah Kedung Jambu Paron Ngawi
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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