8,302 research outputs found
Barbara James
No full textDate:1943Barbara was born in Holdredge, Nebraska in the United States of America in 1943. In 1960 she arrived in Darwin working in a variety of occupations such as a journalist, historian, author, activist, advocate and editor. Barbara wrote 13 books including "No Man's Land" which explored the contributions of women in the Northern Territory. She also received a number of awards including 2001 NT Heritage Award, the 2000 NT Literary Essay Awards and the Chief Minister's Women's Achievement Award in 1999.JournalistHistorianAuthorActivistEditorAmerica
RUOLO DELLE PROTEINE MINORITARIE DEL RETICOLO SARCOPLASMATICO NELL’ACCOPPIAMENTO ECCITAZIONE-CONTRAZIONE DEL MUSCOLO SCHELETRICO
No full textMuscle strength is restored in JP45 and CASQ1 double knock-out mice.
Skeletal muscle constitutes approximately 40% of human body mass, and alterations in muscle mass and in calcium release process are implicated in decrement of strength with age, in neuromuscular diseases, myopaties as well as obesity and diabetes. The action potential leads to muscle fiber contraction and generation of mechanical force in a process called Excitation-Contraction Coupling (ECC), that takes place at the triad, a structure made up of two membrane compartments: transverse tubules, invaginations of sarcolemma and the terminal cisternae of the Sarcoplasmic Reticulum (SR). The main components of ECC machinery are the 1,4 dihydropyridine receptor (DHPR) Cav 1.1 subunit on T tubules, Ryanodine receptor (RyR1) on the SR and Calsequestrin-1, which serve as channel and voltage sensor, Ca2+ release channel and main Ca storage protein of SR respectively. ECC is activated by a bidirectional signalling: depolarization of sarcolemma induces conformational changes of the DHPR that triggers the opening of RyR1 to release calcium ions via an orthograde signalling. Cav1.1 activity is enhanced by a retrograde stimulatory signal delivered by RyR1. The myoplasmic calcium activates the contractile proteins and is subsequently pumped back into the SR by a Ca2+ ATPase (SERCA) pump leading to muscle relaxation. Yet to be identified minor protein components and other proteins aside the DHPR, RyR1 and calsequestrin are essential for the structure and regulation of the machinery involved in ECC. JP45 is a membrane protein interacting with Cav1.1 and the sarcoplasmic reticulum calcium storage protein calsequestrin (CASQ1). We hypothesized that JP45 and CASQ1 form a signalling pathway that modulates Cav1.1 channel activity. We tested this in flexor digitorum brevis (FDB) muscle fibres from JP45 and CASQ1 double knock-out mice (DKO). Our results show that calcium transient evoked by tetanic stimulation in DKO fibres, result from massive calcium influx due to enhanced Cav1.1 channel activity. This enhanced activity restores muscle strength both in vitro and in vivo.
Downregulation of EC uncoupling in aged JP45 KO mice.
The decline in muscular strength with age, termed sarcopenia, is caused largely by a loss of total muscle mass - but also a disproportionate loss of strength. The loss of muscle strength in old age is characterized in part by a deficit in Ca2+ release caused by activation of DHPR, a phenomenon known as excitation-contraction uncoupling (ECU). On the basis of our previous data showing that ablation of JP45 results in a significant loss of muscle strength in 3 months old mice, we hypothesized that ablation of JP45 expression will result in more marked muscle weakness in JP45KO than WT mice with aging. To our surprise however, we found that JP45KO exhibit sustained in vivo and in vitro skeletal muscle force with no further decline in ECC with aging. Calorie restriction induced by the absence of expression of JP45 in specific areas of the brain (nucleus of the solitary tract, area postrema and nucleus arcuate) associated with central regulation of food intake lead to a significant decrease of body weight in JP45KO compared with WT. The latter event inhibited downregulatation of the expression of DHPR which in turn prevented worsening of the EC uncoupling in aged JP45KO mice.
Peroxisome proliferator-activated receptor-γ coactivator-1α (PCG-1α) affects calcium signals in skeletal muscle.
Peroxisome proliferator-activated receptor-γ coactivator-1α (PCG-1α) is implicated in muscle plasticity promoting fibre switching towards oxidative activity. Since fibre type switch involves the activation of calcium dependent transcriptional factors, we analysed the effect of PGC-1α on calcium handling in muscles overexpressing the coactivator. We demonstrate that PGC-1α causes a reduction of maximal muscle force in vivo and ex vivo by diminishing the expression of calcium release molecules and altering calcium release and uptake process. Furthermore, we demonstrate that PGC-1α increases resistance to fatigue and drives fibre type switching partly through remodelling of calcium transients.
Expression of retinaldehyde in skeletal muscle.
We identified the primary structure and role of SRP35, a novel minor protein component of the sarcoplasmic reticulum. We show that SRP35 is a transmembrane component of the SR, is located near the SERCA Ca2+ATPase and is a short-chain dehydrogenase/reductase belonging to the DHRS7C subfamily. We demonstrated that retinol is the substrate of SRP35, since its transient overexpression leads to an increased production of all-trans-retinaldehyde. We show that transfection of C2C12 myotubes with the fusion protein encoding SRP35-EYFP, or adding retinoic acid to C2C12 cell culture, results in a decrease of calcium released by RyR1 and a significant reduction of RyR1 protein expression.
The definition of the role of minor components which make up the ECC molecular machinery is important not only to understand how mutations in genes involved in calcium homeostasis cause myopathies, but also to define new therapeutic targets for innovative strategies aimed to treat neuromuscular disorders linked to defect of EC coupling
Barbara Ras - Sowell Conference 2017
No full textBarbara Ras, San Antonio, Poet, author of "Bite Every Sorrow" and "The Last Skin
Exclusive interview with author Barbara Kingsolver
No full textExclusive interview with author Barbara Kingsolver for her 2018 novel *Unsheltered
Dataset for publication: Post‐war architecture and urban planning as means of reinventing Opole’s past and identity
No full textThe collection includes files related to the publication: Barbara Szczepańska, Post‐War Architecture and Urban Planning as Means of Reinventing Opole’s Past and Identity, „Urban Planning”, Vol 8, No 1 (2023): Bombed Cities: Legacies of Post-War Planning on the Contemporary Urban and Social Fabric, pp. 266-278, https://doi.org/10.17645/up.v8i1.6079. The collection includes figures used in the publication:Opole_plan A plan of Opole, with areas of Ostrówek (left), Market Square (center) and Central Square (right) highlighted in red. Originally published in: "Guidebook to the city of Opole" ("Przewodnik po mieście Opolu", Opole: Księgarnia Opolska, 1948, https://polona.pl/preview/2f383a4a-5e9e-444d-9e94-366b8ac8610d). Author: Z. Streer. Licence: CC0Opole_Monument to the Opole Silesian Fighters for Freedom A photograph depicting Monument to the Opole Silesian Fighters for Freedom (Pomnik Bojownikom o Wolność Śląska Opolskiego) in Opole. Author: Barbara Szczepańska. Licence: CC0Opole_monument of Kazimierz I Opolczyk A photograph depicting the monument of Kazimierz I Opolczyk in the Market Square in Opole. Author: Barbara Szczepańska. Licence: CC0Opole_Market Square_eastern frontage A photograph depicting eastern frontage of the Market Square in Opole. Author: Barbara Szczepańska. Licence: CC0Opole_Market Square_eastern frontage_before 1945 A photograph depicting eastern frontage of the Market Square in Opole before 1945. Originally published on Wikimedia Commons: https://commons.wikimedia.org/wiki/File:Market_Square_in_Opole,_eastern_frontage.jpg. Author: unknown. Licence: CC0Opole_monument of Frederick the Great A photograph depicting monument of Frederick the Great in Opole, before 1945. Originally published on Wikimedia Commons: https://commons.wikimedia.org/wiki/File:Opole_Oppeln_Denkmal_Friedrich_der_Große.jpg. Author: unknown. Licence: CC0</ul
'A date with Barbara': paracosms of the self in biographies of Barbara Newhall Follett
No full textIn 1927, 13-year-old Barbara Newhall Follett published her first book, the critically acclaimed novel, The House Without Windows and Eepersip's Life There.
Twelve years later, on December 7, 1939, 25-year-old Barbara quarrelled with her husband and left her apartment in Boston with $30 in her pocket, and a notebook. She was never seen again.
The House Without Windows is set in a paracosm (Farksolia) she invented, and ends with the metamorphosis of the titular character into a 'fairy-a wood nymph … invisible for ever to all mortals, save those few who have minds to believe, eyes to see'.
In Barbara's (auto)biography, The Unconscious Autobiography of a Child Genius (1966), written by Harold Grier McCurdy 'in collaboration with Helen Follett' (Barbara's mother), the authors wonder: 'Can we be far wrong in substituting Barbara's name for Eepersip's in the closing scenes of [House Without Windows]?
In this paper, I grapple with the formal and ethical challenges of writing about Barbara Newhall Follett, and the ways her family and others have approached the problem of writing her unresolved life story: a child raised and educated in solitude, a celebrated 'natural' child author, a young woman whose disappearance remains unsolved. The paper will explore the ways in which adults write the stories of children's lives, as nostalgia and fable, as fairytale and paracosmic narrative, and the ways in which Barbara's biographers have, consciously and unconsciously, created biographical concordances, or paracosms of the self, in seeking to make meaning of her life's story
Barbara Ehrenreich: Blood Rites: A New Evolutionary Perspective on Violence
No full textBarbara Ehrenreich, author, social critic and political essayist, discusses the emotional and social aspects of warfare and violence.
Barbara Ehrenreich is an American author and political activist who describes herself as a myth buster by trade” and has been called a veteran muckraker by The New Yorker.During the 1980s and early 1990s she was a prominent figure in the Democratic Socialists of America. She is a widely read and award-winning columnist and essayist, and author of 21 books. Ehrenreich is perhaps best known for her 2001 book Nickel and Dimed: On (Not) Getting By in America
Barbara Dicker Oration 2018 - The phenomenon of hallucinations
No full textThe 2018 Barbara Dicker Oration was presented by Professor Iris Sommer on 13 September 2018. Professor Sommer is a best-selling author and Professor of Cognitive Aspects of Neurological and Psychiatric Disorder at the Department of Neuroscience at the University Medical Center Groningen, Netherlands. Entitled The phenomenon of hallucinations, Professor Sommer offered a holistic view into the research and experiences of hallucinations. It’s actually more common than you might think but what happens in our brains when we hallucinate? And what does this mean for new treatments and interventions
Designer: from author to creative commons
No full textThe essay explores the transformations that have occurred in the role of design, from authorship to networking, sharing and opensource modes
Death, Women, and "Value Production": The Circulation of Hair Strings among the Walpiri of the Central Australian Desert
No full text15 p
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