566 research outputs found
Bolt’s Bunch: Mt Stuart Elementary/ TeachStem Robotics Demonstration
No full textJoin us for a demonstration from the Mt. Stuart Elementary School robotics team who just returned from the 2022 VEX Robotics World Championship in Dallas, Texas. Central Washington University’s TeachSTEM program has supported the robotics club at Mt. Stuart for the last four years, through student internships and volunteering efforts at local competitions, some of which have been held at CWU. The team, named Bolt’s Bunch in honor of their robot, Bolt, is comprised of Emery Klyve, Aspen Moore, and Maddy Bryant. They were awarded the Design Award, granted based on interview skills and a very detailed engineering notebook. Jason Eng—a CWU alumnus, Mt. Stuart P.E. teacher, and robotics team head coach—says the competition builds the core competency of teamwork
“Batch” Kinetics in Flow: Online IR Analysis and Continuous Control
No full textCurrently, kinetic data is either collected under steady-state conditions in flow or by generating time-series data in batch. Batch experiments are generally considered to be more suitable for the generation of kinetic data because of the ability to collect data from many time points in a single experiment. Now, a method that rapidly generates time-series reaction data from flow reactors by continuously manipulating the flow rate and reaction temperature has been developed. This approach makes use of inline IR analysis and an automated microreactor system, which allowed for rapid and tight control of the operating conditions. The conversion/residence time profiles at several temperatures were used to fit parameters to a kinetic model. This method requires significantly less time and a smaller amount of starting material compared to one-at-a-time flow experiments, and thus allows for the rapid generation of kinetic data.Novartis (Firm
A Microfluidic System for the Continuous Recycling of Unmodified Homogeneous Palladium Catalysts through Liquid/Liquid Phase Separation
No full textA prototype microflow system for the continuous recycling of homogeneous catalysts through liquid/liquid phase separation was developed and its effectiveness demonstrated in a challenging palladium-catalyzed hydroxylation reaction. The unmodified catalyst is recycled under standard reaction conditions by using a combination of chemical and engineering methods.Novartis (Firm
Recommended from our members
Kinetics analysis and automated online screening of aminocarbonylation of aryl halides in flow
No full textTemperature, pressure, gas stoichiometry, and residence time were varied to control the yield and product distribution of the palladium-catalyzed aminocarbonylation of aromatic bromides in both a silicon microreactor and a packed-bed tubular reactor. Automation of the system set points and product sampling enabled facile and repeatable reaction analysis with minimal operator supervision. It was observed that the reaction was divided into two temperature regimes. An automated system was used to screen steady-state conditions for offline analysis by gas chromatography to fit a reaction rate model. Additionally, a transient temperature ramp method utilizing online infrared analysis was used, leading to more rapid determination of the reaction activation energy of the lower temperature regimes. The entire reaction spanning both regimes was modeled in good agreement with the experimental data
Introduction. Ruptures in the Everyday
No full textRuptures in the Everyday was jointly written by ATG26, a scholarly collective comprising the following authors:Jonathan Bach, Andrew Stuart Bergerson (lead author), Susanne Beer, Mark E. Blum, Michaela Christ, Cristina Cuevas-Wolf, Mary Fulbrook, Eva Giloi, Thomas Gurr, Jason Johnson, Craig Koslofsky, Dani Kranz, Phil Leask, Wendy Lower, Elissa Mailänder, Josie McLellan, Alexandra Oeser, Steve Ostovich, Will Rall, Leonard Schmieding (lead author), Johannes Schwartz, Sara Ann Sewell, Paul Steege, Maximilian Strnad, Julia Timpe, Heléna TóthInternational audienc
Introduction. Ruptures in the Everyday
No full textRuptures in the Everyday was jointly written by ATG26, a scholarly collective comprising the following authors:Jonathan Bach, Andrew Stuart Bergerson (lead author), Susanne Beer, Mark E. Blum, Michaela Christ, Cristina Cuevas-Wolf, Mary Fulbrook, Eva Giloi, Thomas Gurr, Jason Johnson, Craig Koslofsky, Dani Kranz, Phil Leask, Wendy Lower, Elissa Mailänder, Josie McLellan, Alexandra Oeser, Steve Ostovich, Will Rall, Leonard Schmieding (lead author), Johannes Schwartz, Sara Ann Sewell, Paul Steege, Maximilian Strnad, Julia Timpe, Heléna TóthInternational audienc
Institutions
No full textRuptures in the Everyday was jointly written by ATG26, a scholarly collective comprising the following authors:Jonathan Bach, Andrew Stuart Bergerson (lead author), Susanne Beer, Mark E. Blum, Michaela Christ, Cristina Cuevas-Wolf, Mary Fulbrook, Eva Giloi, Thomas Gurr, Jason Johnson, Craig Koslofsky, Dani Kranz, Phil Leask, Wendy Lower, Elissa Mailänder, Josie McLellan, Alexandra Oeser, Steve Ostovich, Will Rall, Leonard Schmieding (lead author), Johannes Schwartz, Sara Ann Sewell, Paul Steege, Maximilian Strnad, Julia Timpe, Heléna TóthInternational audienc
Institutions
No full textRuptures in the Everyday was jointly written by ATG26, a scholarly collective comprising the following authors:Jonathan Bach, Andrew Stuart Bergerson (lead author), Susanne Beer, Mark E. Blum, Michaela Christ, Cristina Cuevas-Wolf, Mary Fulbrook, Eva Giloi, Thomas Gurr, Jason Johnson, Craig Koslofsky, Dani Kranz, Phil Leask, Wendy Lower, Elissa Mailänder, Josie McLellan, Alexandra Oeser, Steve Ostovich, Will Rall, Leonard Schmieding (lead author), Johannes Schwartz, Sara Ann Sewell, Paul Steege, Maximilian Strnad, Julia Timpe, Heléna TóthInternational audienc
Institutions
No full textRuptures in the Everyday was jointly written by ATG26, a scholarly collective comprising the following authors:Jonathan Bach, Andrew Stuart Bergerson (lead author), Susanne Beer, Mark E. Blum, Michaela Christ, Cristina Cuevas-Wolf, Mary Fulbrook, Eva Giloi, Thomas Gurr, Jason Johnson, Craig Koslofsky, Dani Kranz, Phil Leask, Wendy Lower, Elissa Mailänder, Josie McLellan, Alexandra Oeser, Steve Ostovich, Will Rall, Leonard Schmieding (lead author), Johannes Schwartz, Sara Ann Sewell, Paul Steege, Maximilian Strnad, Julia Timpe, Heléna TóthInternational audienc
DNA fusion gene vaccination mobilizes effective anti-leukemic cytotoxic T lymphocytes from a tolerized repertoire
No full textThe majority of known human tumor-associated antigens derive from non-mutated self proteins. T cell tolerance, essential to prevent autoimmunity, must therefore be cautiously circumvented to generate cytotoxic T cell responses against these targets. Our strategy uses DNA fusion vaccines to activate high levels of peptide-specific CTL. Key foreign sequences from tetanus toxin activate tolerance-breaking CD4+ T cell help. Candidate MHC class Ibinding tumor peptide sequences are fused to the C terminus for optimal processing and presentation. To model performance against a leukemia-associated antigen in a tolerized setting, we constructed a fusion vaccine encoding an immunodominant CTL epitopederived from Friend murine leukemia virus gag protein (FMuLVgag) and vaccinated tolerant FMuLVgag-transgenic (gag-Tg) mice. Vaccination with the construct induced epitopespecificIFN-c-producing CD8+ T cells in normal and gag-Tg mice. The frequency and avidity of activated cells were reduced in gag-Tg mice, and no autoimmune injury resulted. However, these CD8+ T cells did exhibit gag-specific cytotoxicity in vitro and in vivo. Also, epitope-specific CTL killed FBL-3 leukemia cells expressing endogenous FMuLVgag antigen and protected against leukemia challenge in vivo. These results demonstrate a simple strategy to engage anti-microbial T cell help to activate epitope-specific polyclonal CD8+ T cell responses from a residual tolerized repertoire
- …
