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Daughter of fortune: Isabel Allende's popularity from a readership perspective
No full textThe primary aim of this thesis is to explore and critically interrogate Isabel Allende’s popularity cross-culturally in Britain and Spain. It analyses readers’ responses to Allende’s works as well as the discourses surrounding her public representation, an approach that is ‘readerly’ but must also take account of production and text. This approach is intended to further the understanding of Allende’s work which so far has always been analysed from a textual perspective. However, the relationship between Allende’s popularity, her texts, public representation and readers has not been yet analysed in detail.This thesis is innovative in other ways too. Methodologically, it approaches readers through the under studied cultural form of the reading group. It also incorporates a comparative dimension by looking at the reception of Allende in two different cultural contexts: the British and Spanish respectively. Finding out about Allende’s popularity has involved asking readers about their reading experiences as well as analysing the production of discourses around her public representation. Paul Ricoeur’s (1984, 1988) perspective on authorial intentions and readers’ responses to texts helps in understanding the intricacies surrounding what is involved in reading any text. It draws attention to Allende’s and her publishers’ authorial strategies, her ‘strategies of persuasion’ and the specificity of the lives and contexts of British and Spanish reading publics. Equally, this ‘readerly’ approach draws on feminist audience research and primarily on the work of Ien Ang and Janice Radway. Their work with viewers and readers respectively is particularly useful in establishing and developing methodological parameters for the study of reading groups. As a whole, this thesis contributes to the understanding of Allende’s cross-cultural popularity by situating readers at the centre
Los estudios y los estudiantes de jurisprudencia y teólogía tras la unificación de las facultades de leyes y cánones
No full textLa reunión de Leyes y Cánones, que venía materializándose desde los plcines de estudios anteriores, se completa en 1842
con la facultad de Jurisprudencia. Las adaptaciones para los estudiantes de ambas facultades son, ciertamente, ventajosas, especialmente para los canonistas. Por sus conexiones con Cánones, los estudiantes de Teología también se vieron afectados. Los grados académicos también sufren modificaciones. Sin embargo, las medidas
que se adoptaron para la transición, y la inmediata publicación del siguiente plan de estudios, impidieron la aplicación exacta y completa del plan.The creation of the Faculty of Jurisprudence in 1842 completes the unification of the studies of Laws and of Canons that had been taking place in previous programs of studies. The adjustments made in both faculties were clearly advantageous for the students,
particularly for those of the Canons. The students of Theology, because of its inherent connections with Canons, were also
affected by the cheinges. Academic degrees and titles were also modified; nonetheless the measures taken for the transition and the swift publication of the resulting program of study prevented the precise and total application of the program.Publicad
Regulation of adenovirus replication by miR-199 confers a selective oncolytic activity in hepatocellular carcinoma
No full textOncolytic virotherapy represents a growing field of experimental cancer therapy. For safe and effective virotherapy, restricted tissue expression and replication of the virus is desirable. Various methods have been developed to achieve such restricted expression. They included the engineering of viral genomes through the insertion of tissue-specific promoters or genes encoding for tissue specific binding proteins. Here, we employed a new approach based on the use of microRNAs (miRNAs) to achieve tumor-specific viral expression and replication. miRNAs are approximately 22-nucleotide (nt)- long non-coding RNAs that are able to bind the 3’ untranslated regions (UTRs) of homologous target mRNAs and causing either their degradation or translation inhibition. Since miRNA are differentially expressed in cancer versus normal cells, it is theoretically possible to make virus expression restricted to cancer cells in a miRNA-dependent manner.
Several studies have shown that miR-199 is significantly down-regulated in primary hepatocellular carcinoma (HCC) tissue and HCC cell lines. With this notion in mind, we developed a conditionally replication-competent oncolytic adenovirus, Ad-199T, by introducing four copies of miR-199 target sites within the 3′ UTR of the E1A gene, which is essential for adenovirus replication.
In vitro studies of the properties of Ad-199T virus revealed that E1A expression was indeed tightly regulated both at RNA and protein levels depending upon the expression of miR-199. Consequently, Ad-199T could replicate in the HCC derived cells HepG2, negative for miR-199 expression, while its replication was strictly controlled in HepG2-199 cells, which were engineered to express high level of miR-199. A replication-competent miRNA independent Ad-Control was also generated,. Thus, these in vitro studies proved that cytotoxicity of Ad-199T was effective in HCC derived cells, which lacks expression of miR-199, and could be successfully controlled in cells that express miR-199 at high level.
To assess in vivo properties of Ad-199T, we tested an orthotopic tumor model. HepG2 cells were implanted in the liver of newborn B6D2 mice. The cells could survive at least one week in this environment, enough for testing in vivo properties of Ad-199T. These studies revealed that intrahepatic delivery of Ad-199T led to virus replication in HepG2 derived xenograft tumors and a faster removal of cancer cells. Conversely, Ad-199T replication was not detected in normal, miR-199 positive, liver parenchyma.
These results demonstrate that Ad-199T is a conditionally replicative adenovirus (CRAd) miR-199 dependent, with antitumor activity in vivo. This system allows replication of the oncolytic virus in HCC cells and, at the same time, tightly control replication in normal liver tissues, thus avoiding or reducing hepatotoxicity
A Discussion About Writing Fiction and Creative Prose with Isabel Huggan
No full textAward-winning Canadian author Isabel Huggan talks to students about writing, with a focus on fiction and creative non-fiction.Presentation for English 2905 (Introduction to Creative Writing), taught by Dr. Stepanie McKenzie
Expression of microRNAs: miR-23a, miR-27a, miR-181b, miR-203 and miR-210 in neurospheres and adhered cells of cultures of glioblastoma patients
No full textIntrodução: Glioblastomas são as neoplasias primárias malignas mais comuns do sistema nervoso central em adultos, com uma sobrevida baixa a um ano do diagnóstico, devido ao seu alto poder tumorigênico, rápida progressão e invasividade, em parte pela presença de uma subpopulação de células neoplásicas com características de células tronco normais (NSCs) denominadas células tronco tumorais (CSCs) REF. Acredita-se que as CSCs sejam as responsáveis pela gliomagênese, heterogeneidade, progressão e reestabelecimento tumoral. Estudos demonstram que muitos microRNAs apresentam níveis de expressão alterados nos tumores cerebrais e têm sido associados a metástase, à patogênese dos glioblastomas, à expressão de oncogenes e de genes supressores de tumor em CSCs, incluído o GBM, mediante diversos mecanismos moleculares REF. Objetivo: Avaliar a expressão dos miRNAs miR-23a, miR-27a, miR-181b, miR-203 e miR-210, envolvidos na patogênese do glioma e metástase cerebral, comparando sua expressão em Células Aderidas e Neuroesferas. Materiais e Métodos: Foram usadas culturas celulares de dez pacientes adultos com glioblastoma sendo verificada a expressão dos microRNAs utilizando a PCR em tempo real. Resultados e Conclusões: Não foram observadas diferenças estatisticamente significativas na expressão dos microRNAs miR-23a, miR-27a, miR-181b, miR-203 e miR-210 quando comparados em culturas de células aderidas e neuroesferas de tecidos de glioblastoma. Entretanto, apresentaram-se hiperexpressos nas células aderidas nos microRNAs: miR-27a, miR-181b e miR-210 e em neuroesferas no miR 203.Introduction: Glioblastomas are the most common primary malignant neoplasms of the central nervous system in adults, with a one-year low survival of the diagnosis, due to its high tumorigenic power, rapid progression and invasiveness, in part by the presence of a subpopulation of neoplastic cells with characteristics of normal stem cells (NSCs) called cancer stem cells (CSCs). CSCs are believed to be responsible for gliomagenesis, heterogeneity, progression and tumor reestablishment. Studies have shown that many microRNAs have altered levels of expression in brain tumors and have been associated with metastasis, the pathogenesis of glioblastomas, the expression of oncogenes and tumor suppressor genes in CSCs, including GBM, by various molecular mechanisms. Objective: To evaluate the expression of the miRNAs miR-23a, miR-27a, miR-181b, miR-203 and miR-210, involved in the pathogenesis of glioma and cerebral metastasis, comparing its expression in Adhered Cells and Neurospheres. Materials and Methods: Cell cultures of ten adult patients with glioblastoma were used and the expression of microRNAs was verified using real-time PCR. Results and Conclusions: No statistically significant differences were found in the expression of miR-23a, miR-27a, miR-181b, miR-203 and miR-210 microRNAs when compared in cultures of adhered cells and glioblastoma tissue neurospheres. However, hyperexpressions were observed in the adhered cells in the miR-203, miR-27a, miR-181b and miR-210 and neurospheres in miR-203
Humanismo y Reforma en la corte renacentista de Isabel de Vilamarí : Escipión Capece y sus lectoras
No full textDurante la primera mitad del siglo XVI y en la corte salernitana del último príncipe de la casa Sanseverino y de su esposa, Isabel de Vilamarí (noble señora de origen catalán) se desarrolló un intenso clima intelectual. Allí se congregaron artistas y humanistas italianos y españoles. En este ambiente de intercambio cultural, atento en participar en las ideas de la Reforma que se difundió en Nápoles gracias a B. Ochino y a Valdés, nace el poema De principiis rerum del último académico pontaniano: Escipión Capece. En esta obra no sólo se rastrean motivos lucrecianos y virgilianos sino también el influjo de los tratados cosmológicos de Pontano. En este estudio, la autora propone el análisis de la figura y de la obra de Capece a través de sus lectoras: Isabel de Vilamarí y las mujeres cultas de su corte.During the first half of sixteenth century and in the Salernitan court of the last prince Sanseverino and his wife Isabel de Vilamarí (a lady coming from a noble Catalan family) an intense intellectual climate developed. Italian and Spanish artists and humanists met there. In this environment of cultural exchange, that shared in the Reform ideas divulged in Naples by B. Ochino and Valdés, Scipione Capece (the last member of the Pontanian Academy) writes his poem De principiis rerum. In his book Capece uses Latin literature (Vergil and Lucretius mainly) and Pontano's treatises on cosmology. The author of this paper studies Scipione Capece through his female readership: Isabel de Vilamarí and the learned women from her court
Humanismo y Reforma en la corte renacentista de Isabel de Vilamarí : Escipión Capece y sus lectoras
No full textDurante la primera mitad del siglo XVI y en la corte salernitana del último príncipe de la casa Sanseverino y de su esposa, Isabel de Vilamarí (noble señora de origen catalán) se desarrolló un intenso clima intelectual. Allí se congregaron artistas y humanistas italianos y españoles. En este ambiente de intercambio cultural, atento en participar en las ideas de la Reforma que se difundió en Nápoles gracias a B. Ochino y a Valdés, nace el poema De principiis rerum del último académico pontaniano: Escipión Capece. En esta obra no sólo se rastrean motivos lucrecianos y virgilianos sino también el influjo de los tratados cosmológicos de Pontano. En este estudio, la autora propone el análisis de la figura y de la obra de Capece a través de sus lectoras: Isabel de Vilamarí y las mujeres cultas de su corte.During the first half of sixteenth century and in the Salernitan court of the last prince Sanseverino and his wife Isabel de Vilamarí (a lady coming from a noble Catalan family) an intense intellectual climate developed. Italian and Spanish artists and humanists met there. In this environment of cultural exchange, that shared in the Reform ideas divulged in Naples by B. Ochino and Valdés, Scipione Capece (the last member of the Pontanian Academy) writes his poem De principiis rerum. In his book Capece uses Latin literature (Vergil and Lucretius mainly) and Pontano's treatises on cosmology. The author of this paper studies Scipione Capece through his female readership: Isabel de Vilamarí and the learned women from her court
Isabel Allende recuerdos para un cuento = Isabel Allende : memories for a story
No full textA simple description of the childhood and youth of the Chilean author Isabel Allend
Reforma e contra - reforma penal (1984-1996): uma ilusão ... que sobrevive
No full textDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Jurídicas. Programa de Pós-Graduação em Direit
DataSheet_1_Small RNA Expression Profiling Reveals hsa-miR-181d-5p Downregulation Associated With TNF-α Overexpression in Sjögren’s Syndrome Patients.doc
No full textMicroRNAs (miRNAs) are small non-coding RNAs (sRNA), that alter gene expression by binding to target messenger RNAs (mRNAs) and repressing translation. Dysregulated miRNA expression has been implicated in the pathogenesis of autoimmune diseases such as Sjögren’s syndrome (SS). The aim of this study was to characterize the global profile of sRNAs in labial salivary glands (LSG) from SS-patients and to validate potential miRNA candidates implicated in glandular inflammation. LSG from 21 SS-patients and 9 sicca controls were analyzed. A global next generation sequencing (NGS)-based sRNA profiling approach was employed to identify direct targets whereby differentially expressed miRNAs were predicted using bioinformatics tools. miRNA levels were validated by TaqMan and target mRNA levels were determined by quantitative real-time PCR. We also performed in vitro assays using recombinant TNF-α. NGS shows that ~30% of sRNAs were miRNAs. In comparison with samples from sicca controls, four miRNAs were found differentially expressed in LSG from SS-patients with low focus score (LFS) and 18 from SS-patients with high focus score (HFS). The miRNA with the most significant changes identified by NGS was hsa-miR-181d-5p and downregulation was confirmed by TaqMan analysis. Levels of TNF-α mRNA, a direct target of hsa-miR-181d-5p, were significantly increased and negatively correlated with hsa-miR-181d-5p presence. Moreover, positive correlations between TNF-α transcript levels, focus score, ESSDAI, and autoantibody levels were also detected. Furthermore, TNF-α stimulation decreased hsa-miR-181d-5p levels in vitro. Downregulation of hsa-miR-181d-5p in LSG from SS-patients could contribute to the glandular pro-inflammatory environment by deregulation of its direct target TNF-α. Further dissection of the pathophysiological mechanisms underlying the hsa-miR-181d-5p-mediated action in inflammatory conditions could be useful to evaluate the benefits of increasing hsa-miR-181d-5p levels for restoration of salivary gland epithelial cell architecture and function.</p
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