196,418 research outputs found
Genomic Typing of Human Red Cell Miltenberger Glycophorins in a Taiwanese Population
No full text[[abstract]]BACKGROUND: Antigens in the human red cell Miltenberger series are glycophorin variants of the MN (MNS) blood group system that are due to the rearrangement of glycophorin A (GPA) and glycophorin B (GPB) genes.
STUDY DESIGN AND METHODS: Taking advantage of the differences between the GPA and GPB genes, a polymerase chain reaction-based method was developed to detect all the Miltenberger glycophorin variants and Sta subtype. GPA- and GPB-specific primers were used to amplify the GPA or GPB gene, and the amplified products were used to recognize the different hybrid genes after restriction enzyme digestions.
RESULTS: Among 264 Taiwanese subjects studied, Mi.III and Sta are the most common types of Miltenberger variants found. Mi.III was present in 13 (4.92%) of 264, and Sta was found in 8 (3.03%) of 264; 1 case (0.4%) of Mi.V was also identified from the study group.
CONCLUSION: This is the first polymerase chain reaction-based method of detecting most of the Miltenberger variants and Sta. The genomic typing results were confirmed by control DNA of identified Miltenberger phenotypes. The prevalence rates of Mi.III and Sta in this study were also consistent with other previous reports using different methods.
Human glycophorins are the major sialoglyco-proteins expressed on the red cell membrane that carry the antigens of the MN (MNS) blood group system.1 They are encoded by a gene family that includes at least three common members: glycophorin A (GPA), glycophorin B (GPB), and glycophorin E (GPE). These genes are present as a single copy in the haploid genome, and they share extensive sequence homology in both coding and noncoding regions, except for the extracellular and cytoplasmic domains. The GPA (α) gene contains seven actively expressed exons, while the GPB (δ) and GPE (ɛ) genes lack exons for the cytoplasmic domains. The third exon of GPB and the third and fourth exons of GPE are silent pseudo-exons because of the inactivation of their donor-splice sites by point mutations at the first position of GT dinucleotides.2–4
Studies of genetic variation in the glycophorin genes have shown that variant genes frequently are hybrids from parts of the parental genes arranged in various configurations.4 This suggests that antigenic variation in the human MN (MNS) blood group system occurs predominantly through DNA recombination. The hybrids can be assembled in four basic configurations with respect to the arrangement of parent genes. They are GPA-B, GPB-A, GPA-B-A, and GPB-A-B, which are the results of nucleotide (nt) sequence homology and special DNA motifs inherent from the parent genes. Analysis of all the recombinations shows that all the events occur at the MN (MNS) locus within a genomic region of approximately 4 kb that spans exons of the parent genes encoding the extracellular domain. Moreover, in 18 of 20 variants, genetic exchanges are confined to a 2-kb region spanning from exon II through exon IV.5
Antigens in the Miltenberger series are variants of the MN (MNS) blood group system. As a subsystem of the MN blood group, the antigens in the Miltenberger series all occur through gene rearrangements during the recombination of the parental genes.5,6 These variants were determined by using serologic and biochemical methods in the past,7–10 and some genetics methods have been used for their detection.11–16 In this study, we developed a comprehensive polymerase chain reaction (PCR)-based method of detecting most of the Miltenberger variants, and then we used this new technique to screen for Miltenberger variants among a Taiwanese population to understand the prevalence of these variants in that group
Dr. Duane M. Jackson, Morehouse College, July 2011
No full textThis video is a conversation with Dr. Duane M. Jackson. Dr. Jackson talks about his paper, "Recall and the Serial Position Effect: The Role of Primacy and Recency on Accounting Students' Performance." Jackie Daniel, AUC Woodruff Library, is the interviewer
A literature review on school child sexual abuse prevention
No full textPlan BChild sexual abuse is a problem occurring at alarming rates in our society. Although the number of substantiated cases of child sexual abuse in the United States is startling, these astronomical statistics do not represent the total number of child sexual victimizations. Most children do not disclose their abuse, with reasons varying from embarrassment, a sense of normalization surrounding the abuse, and fear of consequences if they disclose the abuse (Palmer, Brown, Rae-Grant, & Laughlin, 1999). In the late 1970s schools started implementing child sexual abuse prevention programs to help with this problem (Kohl, 1993, cited in Roberts & Miltenberger, 1999). School child sexual abuse prevention programs help promote disclosure of past abuse and prevent future abuse of students. The purpose of this research project was to examine the literature on child sexual abuse prevention programs in schools to see what effects these programs have. Areas reviewed in this paper include facts on child sexual abuse, what school child sexual abuse prevention programs look like, two specific curricula, and evaluations of these programs. Research shows that most school districts offer some sort of child sexual abuse prevention program (Kohl, 1993, cited in Roberts & Miltenberger, 1999). Since school child sexual abuse prevention programs vary, evaluations of such programs differ. Overall, prevention programs appear to offer far more positive than negative affects on children. In general, students who were involved with these programs tend to have a better understanding of various aspects on child sexual abuse. Programs that involve the students in activities and occur repeatedly over time seem to offer the best results (Davis & Gidycz, 2000)
"Reflections on the subject of Emigration from Europe with a view to Settlement in the United States" By M. Carey.
No full text"Reflections on the subject of Emigration from Europe with a view to Settlement in the United States: containing bried sketches of the moral and political character of those states.
By M. Carey, member of the American philosophical, and of the American Antiquarian Society, and author of The Olive Branch, Cindiciae Hibernicae, essays on banking, on political economy, and on internal improvement.
To which are now added the English editor's comments on the subject; together with Important Advice to Emigrants, and Cautions Against Impositions Practiced in the Outports
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Dr. Glendon Swarthout
No full textHosted by Roger M. Busfield, MSU Assistant Professor of Speech and Theater, Meet the Author is designed to introduce a general audience to a contemporary author and their work through in-depth interviews. This episode features a conversation between Dr. Glendon Swarthout, prolific author and English professor at MSU, and assistant professors Sam S. Baskett and Theodore B. Strandness
Simulation of thermal plant optimization and hydraulic aspects of thermal distribution loops for large campuses
No full textFollowing an introduction, the author describes Texas A&M University and its utilities system. After that, the author presents how to construct simulation models for chilled water and heating hot water distribution systems. The simulation model was used in a $2.3 million Ross Street chilled water pipe replacement project at Texas A&M University. A second project conducted at the University of Texas at San Antonio was used as an example to demonstrate how to identify and design an optimal distribution system by using a simulation model. The author found that the minor losses of these closed loop thermal distribution systems are significantly higher than potable water distribution systems. In the second part of the report, the author presents the latest development of software called the Plant Optimization Program, which can simulate cogeneration plant operation, estimate its operation cost and provide optimized operation suggestions. The author also developed detailed simulation models for a gas turbine and heat recovery steam generator and identified significant potential savings. Finally, the author also used a steam turbine as an example to present a multi-regression method on constructing simulation models by using basic statistics and optimization algorithms. This report presents a survey of the author??s working experience at the Energy Systems Laboratory (ESL) at Texas A&M University during the period of January 2002 through March 2004. The purpose of the above work was to allow the author to become familiar with the practice of engineering. The result is that the author knows how to complete a project from start to finish and understands how both technical and nontechnical aspects of a project need to be considered in order to ensure a quality deliverable and bring a project to successful completion. This report concludes that the objectives of the internship were successfully accomplished and that the requirements for the degree of Degree of Engineering have been satisfied
Intern experience at CH���M Hill, Inc.: an internship report
No full textIncludes author's vita"Submitted to the College of Engineering of Texas A&M University in partial
fulfillment of the requirement for the degree of Doctor of Engineering."Includes bibliographical referencesA review of the author's internship experience with CH���M HILL, Inc.
during the period September 1975 through May 1976 is presented. During this nine month
internship the author worked as an Engineer II in the Industrial Processes discipline of this
large consulting engineering firm... The author's prime responsibility was as one of three
lead design engineers on the design of a large wastewater treatment facility for a pulp mill
in Hoquiam, Washington owned by ITT Rayonier Inc. The work generally consisted of the design
of individual treatment units and associated piping and pumping. The purpose of the project
was to provide wastewater treatment capabilities that would satisfy the effluent limitations
(standards) imposed upon the mill by the State of Washington Department of Ecology and the
U.S. Environmental Protection Agency. The author's assignment also entailed necessary
interaction with the project manager and other CH���M HILL design engineers and support staff
members, the client's representatives, and representatives of two other consulting engineering
firms working on the project. Thus, the internship position at CH���M HILL provided considerable
experience coordinating the author's work with the work of other engineers, guiding the design
and administrative efforts of a support staff, and interacting regularly with the client and
other consulting firms. This broad exposure to a variety of engineering and organizational
problems provided a valuable educational experience
Transition to turbulence in a qblique shock-wave/boundary-layer interaction at M=15
No full textDirect numerical simulations are carried out for different forcing techniques to trigger transition during the interaction between an oblique shock-wave and a laminar boundary-layer at M = 1.5. Three forcing methods are used: a) forcing of oblique unstable modes, whose shape and behaviour are determined by the local linear stability theory, b) broadband free-stream acoustic disturbances, and c) a cold plasma flow control device. While the oblique-mode breakdown is dominant for low-amplitude forcing, long streaky structures drive the transition process in a high-amplitude disturbance environment. LES are also performed on the experimental setup by the Institute of Theoretical and Applied Mechanics (ITAM) from Novosibirsk State University with cold plasma actuation. As well as the disturbance type, the effect of Reynolds number and forcing amplitude will be investigated
Letter from Cedrick M. Shimo to the Office of Redress Administration, June 4, 1991
No full textA letter from Cedrick M. Shimo to the Office of Redress Administration arguing that John Y. Udaka is entitled to a redress payment.These materials are from box 73 and 74 of the Frank Chin Papers. The Frank Chin Papers contain personal and professional correspondence between Frank Chin and Michi Weglyn relating to particular projects on which either author was working as well as files related to the Day of Remembrance Tribute to Michi Weglyn
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