1,721,096 research outputs found
Stability of wide-graded rubble mounds
Full text linkThe reshaping of temporary rubble mounds like the core of breakwaters or reclamation bunds is often a concern for contractorsi n the construction stages of marine structures. The formulas found in literature for the prediction of such behavior are few, and they do not provide clear insight on the influence of relevant parameters, in particular the small dimensions and wide stone-size gradation of the material involved, usually consisting of quarry run or resulting from dredging. The previous research in the field of dynamic stability focused on berm breakwaters and gravel beaches. These two typologies of structures define the range to which the rubble mounds considered in this study generally belong. An overview on the design tools provided by the technical literature shows that, whenever the grading was included as a governing parameter, some influence was recognized in the characteristics of the structure (e.g. the permeability) and in the dynamism of the different fractions of stone sizes. However, very wide ranges of the parameter grading were never investigated and a specific analysis in this direction constitutes the main significance of this study. The Delft University of Technology provided the laboratory facilities to carry out physical model tests on a wide graded rubble mound structure representative of the core of a breakwater. The parameter D85/D15, describing the stone-size gradation of the construction material, was varied between the values 2.71 and 17.7, and two different seaward slopes of the model structure were also tested. The reshaped cross-shore profiles measured during the tests showed how if the grading increases the stability of the structure is reduced. This is not always in accordance with the findings of previous researchers, showing how the extrapolation of existing empirical formulas to structures with high values of the ratio D85/D15 do not give reliable results. Instead, the formulas given by van de Meer (1992) to estimate the whole reshaped profile of a dynamic slope predict with good agreement the shape of the measured profiles, although the physical model shows a larger horizontal extension of the displacements. This difference is governed by the grading, being more noticeable as this parameter increases. This result leads to the definition of new formulas, some of them being modifications of the ones given by van der Meer, to describe the geometry of a reshaped profile. The formulas, all including the parameter grading, are derived through curve fitting of the measured data. Also a formula for the direct estimation of the crest recession is given. As a final step, a simple numerical model is proposed in which the new formulas are implemented, constituting a quick way to assess the shape of a slope after a wave attack. As a suggestion for further utilization of the results of physical modeling, a brief comparison is also carried out between the output of the tests and the prediction of the numerical model XBeach (developed mainly at UNESCO-IHE). In conclusion, this research points out how the formulas provided by the technical literature are not reliable in representing the effects of a very wide stone-size gradation in the stability of a rubble mound structure. Physical model tests proved to be a suited way to investigate these effects, as the nature of the phenomena who play a role in the stability does not allow a simple analytical representation. The tests carried out within the present study lead to the implementation of a numerical model of practical use for engineers and contractors: further investigations through laboratory tests are recommended to validate and extend the findings of this study. Another proposed direction for further research is the comparison between the results of physical model tests and the output of numerical models.Hydraulic EngineeringCivil Engineering and Geoscience
Catalysis and electron transfer in protein crystals: the binary and ternary complexes of methylamine dehydrogenase with electron acceptors
No full textPolarized absorption microspectrophotometry has been used to detect catalysis and intermolecular electron transfer in single crystals of two multiprotein complexes: (1) the binary complex between Paracoccus denitrificans methylamine dehydrogenase, which contains tryptophan-tryptophylquinone (TTQ) as a cofactor, and its redox partner, the blue copper protein amicyanin; (2) the ternary complex between the same two proteins and cytochrome c-551i. Continuous wave electron paramagnetic resonance has been used to compare the state of copper in polycrystalline powders of the two systems. While catalysis and intermolecular electron transfer from reduced TTQ to copper are too fast to be accessible to our measurements, heme reduction occurs over a period of several minutes. The observed rate constant is about four orders of magnitude lower than in solution. The analysis of the temperature dependence of this apparent constant provides values for the parameters H-AB, related to electronic coupling between the two centers, and lambda, the reorganizational energy, that are compatible with electron transfer being the rate-determining step. From these parameters and the known distance between copper and heme, it is possible to calculate the parameter beta, which depends on the nature of the intervening medium, obtaining a value typical of electron transfer across a protein matrix. These findings suggest that the ternary complex in solution might achieve a higher efficiency than the rigid crystal structure thanks to an as yet unidentified role of protein dynamics
The effects of acid and alkaline solutions on cut marks and on the structure of bone : an experimental study on porcine ribs
No full textAmong taphonomical modifications during decomposition processes, little is known about the action of high or low pH to human tissues and bones. Moreover, acid or basic solutions are seldom used to ease decomposition and wrecking of the body. In this study a total of 60 samples of porcine bones on which two cut marks were produced before the beginning of the experiment, were put in six different solutions with different pH (1, 3, 5, 9, 12, 14) and analyzed every five days over a period of 70. days. Surveys were carried out macroscopically, with stereomicroscopy and with light microscopy on thin sections. Only the specimens exposed to extremely acid (12) pH showed evident modifications of the bone's structure, as witnessed by the analyses with stereomicroscopy as well. Many samples showed a detachment of the periosteum; cut marks became soon unrecognizable with pH 14 but still detectable in all the other samples. The information gained from the present study can be of great help in detecting the exposure of human tissues to high or low environmental pH and in understanding the effects that these solutions can exert on human bones
Electron transfer in crystals of the binary and ternary complexes of methylamine dehydrogenase with amicyanin and cytochrome c551i as detected by EPR spectroscopty
No full textEPR studies of the methylamine dehydrogenase (MADH)-amicyanin and MADH-amicyanin-cytochrome c551i crystalline complexes have been performed on randomly oriented microcrystals before and after exposure to the substrate, methylamine, as a function of pH. The results show that EPR signals from the redox centers present in the various proteins can be observed simultaneously. These results complement and extend earlier studies of the complexes under similar conditions that utilized single-crystal polarized absorption microspectrophotometry. The binary complex shows a blue copper axial signal, characteristic of oxidized amicyanin. After reaction of substrate with the MADH coenzyme tryptophan tryptophylquinone (TTQ), the binary complex exhibits an equilibrium mixture of oxidized copper/reduced TTQ and reduced copper/TTQ. radical, whose ratio is dependent on the pH. In the oxidized ternary complex, the same copper axial signal is observed superimposed on the low-spin ferric heme features characteristic of oxidized cytochrome c551i. After addition of substrate to the ternary complex, a decrease of the copper signal is observed, concomitant with the appearance of the radical signal derived from the semiquinone form of TTQ. The equilibrium distribution of electrons between TTQ and copper as a function of pH is similar to that observed for the binary complex. This result was essential to establish that the copper center retains its function within the crystalline ternary complex. At high pH, with time the low-spin heme EPR features disappear and the spectrum indicates that full reduction of the complex by substrate has occurred
Corrigendum to "The effects of acid and alkaline solutions on cut marks and on the structure of bone: An experimental study on porcine ribs" [Leg. Med. 17 (6) (2015) 503-508]
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Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
P2Y2 receptor antagonists as anti-allodynic agents in acute and sub-chronic trigeminal sensitization: role of satellite glial cells
No full textTrigeminal (TG) pain often lacks a satisfactory pharmacological control. A better understanding of the molecular cross-talk between TG neurons and surrounding satellite glial cells (SGCs) could help identifying innovative targets for the development of more effective analgesics. We have previously demonstrated that neuronal pro-algogenic mediators upregulate G protein-coupled nucleotide P2Y receptors (P2YRs) expressed by TG SGCs in vitro. Here, we have identified the specific P2YR subtypes involved (i.e., the ADP-sensitive P2Y1R and the UTP-responsive P2Y2R subtypes), and demonstrated the contribution of neuron-derived prostaglandins to their upregulation. Next, we have translated these data to an in vivo model of TG pain (namely, rats injected with Complete Freund's adjuvant in the temporomandibular joint), by demonstrating activation of SGCs and upregulation of P2Y1R and P2Y2R in the ipsi-lateral TG. To unequivocally link P2YRs to the development of facial allodynia, we treated animals with various purinergic antagonists. The selective P2Y2R antagonist AR-C118925 completely inhibited SGCs activation, exerted a potent anti-allodynic effect that lasted over time, and was still effective when administration was started 6-days post induction of allodynia, i.e. under subchronic pain conditions. Conversely, the selective P2Y1R antagonist MRS2179 was completely ineffective. Moreover, similarly to the anti-inflammatory drug acetylsalicylic acid and the known anti-migraine agent sumatriptan, the P2X/P2Y nonselective antagonist PPADS was only partially effective, and completely lost its activity under sub-chronic conditions. Taken together, our results highlight glial P2Y2Rs as potential "druggable" targets for the successful management of TG-related pain. GLIA 2015;63:1256-1269 Main Points: Pro-algogenic mediators modulate P2Y2 purinergic receptors on trigeminal satellite glial cells in vitro; Selective blockade of P2Y2 receptors reverts allodynia and satellite glial cell activation in an in vivo model of trigeminal sensitization
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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