3,644 research outputs found
Miastenia gravis: perfil clínico de uma série de 20 casos.
No full textTrabalho de Conclusão de Curso - Universidade Federal de Santa Catarina. Curso de Medicina. Dapartamento de Clínica Cirúrgica
Evaluation of phylogenetic markers suitable for Planktothrix spp. discrimination
No full textIn Portugal, potentially toxic cyanobacteria from Planktothrix genus have become frequently observed in freshwater reservoirs. Identification of Planktothrix species through optical microscopy is complicated due to limited morphological differences among them.
The aim of this work was to determine the most suitable phylogenetic markers that could be used for the molecular identification of Planktothrix species. In order to do so, several genes of interest were selected: rpoB, rpoC1, cpcA, cpcB, rbcX, 16S rRNA genes and 16S rRNA–tRNAIle–tRNAAla-23S rRNA internal transcribed spacer (ITS), and their sequences retrieved from public databases.
Phylogenetic analysis showed that 16S rRNA, cpcA, rbcX genes and ITS region trees do not allow a clear discrimination of Planktothrix species, however cpcB and rpoB seemed putative suitable phylogenetic markers for Planktothrix species identification. The applicability of these markers was then evaluated in 20 Planktothrix isolates, isolated over the years from several Portuguese freshwater reservoirs and maintained in the Estela Sousa e Silva Algae Culture Collection (ESSACC). The selected genes, cpcB and rpoB, were amplified by PCR and sequenced and the resulting trees compared with the phylogenetic clustering obtained with our previously characterized rpoC1 phylogenies. The phylogenetic analyses, based on the three gene regions, revealed that Planktothrix isolates analyzed in this study could be phylogenetically resolved into their corresponding species.
This work contributes for the discussion of the appropriate genes that can be used in phylogenetic identification of Planktothrix species.The authors would like to thank the Ph.D research grant SFRH/BD65706/2009 to Catarina Churro and the financial support through project PPCDT/AMB/60675/2006 both given by Fundação para a Ciência e Tecnologia (Portugal)
Advanced Systems Theory Applied to AMB Systems
No full textIn this thesis we look at the application of advanced systems theoretic algorithms for an experimental Active Magnetic Bearing System (AMB). These systems are inherently unstable and therefore require a feedback controller. Accurate modeling of these systems with input-output data is hindered due to the strong correlation between the inputs and noise. Recently a predictor based subspace identification algorithm has been developed specially catered to data from closed-loop experiments. We use this algorithm to build models for a flexible AMB spindle. In order to improve the accuracy of the model, we use frequency domain method which leads to a nonlinear optimization problem. We consider a variety of problem scenarios for designing controllers for rejecting disturbances that are caused due to mass imbalances. For ensuring performance against an uncertainty in the rotational speed we use a robust synthesis algorithm that is known to be convex. We synthesize H-infinity controllers and observe that for a model with flexible dynamics the resulting controllers are unstable. To realize the performance benefits of an unstable controller, we use a switching method based on the Youla parameters of the stabilizing controllers. We also design and implement LPV controllers for rejecting disturbances over a range of rotational speeds.Delft Center for Systems and ControlMechanical, Maritime and Materials Engineerin
Cytotoxicity of AmB, MFLB-AmB or MFLB toward human mesangial cells and isolated mouse peritoneal macrophages.
No full textConcentration of amphotericin B (left vertical axis) in AmB or MFLB-AmB or iron concentration (right vertical axis–panel B) in MFLB required to reduce by half the cell population (LC50) at different time windows, A) human mesangial cells and B) mice peritoneal macrophage cells 5×104 cells were treated in culture medium with AmB alone or with the MFLB-AmB complex rangin AmB concentration from 0.25 to 1.0 μg/mL at 37°C for 6, 12 or 24 hours. After indicated times, cells were incubated with MTT (0.5 mg/mL- Invitrogen, Grant Island, NY, USA) for 4 hours at 37°C. Formazan crystals were dissolved in 200 μL of dimethyl sulfoxide (DMSO). Cell viability was assessed by measuring the absorbance at 590 nm, using a microplate reader. LC50 values were estimated following Probit Analyses. (AmB = amphotericin B, MFLB-AmB = nanosized magnetite surface-functionalized with a bilayer of lauric acid conjugated with amphotericin B, MFLB = nanosized magnetite surface-functionalized with a bilayer of lauric acid treated at the same corresponding iron concentration of MFLB-AmB, ranging from 0.42 to 204.70 μg of Fe/mL).</p
Intervenció a l’aula d’Educació Primària amb alumnes amb TDA-H: repositori digital de propostes d’implementació
No full text[cat] Aquest Treball de Fi de Grau té com a objectiu compilar tot un conjunt de pautes,
activitats i recursos, a través d’una proposta fonamentada en autors actuals i especialitzats en
l’atenció als nens amb TDA-H de l’Educació Primària. Aquestes s’emmarcaran en el
constructivisme i en el respecte per les intel·ligències múltiples.
Tot es compartirà amb la comunitat educativa amb una plataforma web de creació
pròpia adreçada als tutors, sense excloure a les famílies que en vulguin fer ús.[eng] This final degree project aims to compile a set of guidelines, activities and resources,
through a proposal based on current and author specializing in attention to Primary Education
children with ADD(H). This is put in a constructivism frame and respects multiple
intelligences.
Everything will be shared with the school community through a web platform
addressed to tutors, without excluding families who want to use it
Fungal burden as assessed by CFU from mice lungs treated with AmB, MFLB-AmB, and MFLB.
No full textLung fragments were weighed, macerated and homogenized in 1 mL cold, sterile PBS. Part of homogenized pulmonary tissue was plated on Petri dishes containing BHI (Brain Heart Infusion) medium, supplemented with 4% (v/v) horse serum, 5% (v/v) supernatant isolated from the infiltrated culture Pb192, and 40 mg/L gentamicin. CFU per gram of lung were determined seven days after plating with incubation at 37°C. Negative Control Group = animals uninfected; Positive Control, MFLB, AmB and MFLB-AmB Groups = animals infected with Paracoccidioides brasiliensis; (AmB = Amphotericin B; MFLB-AmB = nanosized magnetite surface-functionalized with a bilayer of lauric acid conjugated with AmB; MFLB = nanosized magnetite surface-functionalized with a bilayer of lauric acid).</p
DNA fragmentation in bone marrow cells from mice treated with AmB, MFLB-AmB or MFLB.
No full textFemale BALB/c mice uninfected (NC–negative control) and infected with Paracoccidioides brasiliensis, treated with PBS (PC–positive control), AmB (Amphotericin B), MFLB-AmB (nanosized magnetite surface-functionalized with a bilayer of lauric acid conjugated with Amphotericin B) and MFLB (nanosized magnetite surface-functionalized with a bilayer of lauric acid) for 30 or 60 days.</p
Author profiling resources
No full textEl zip conté tots els recursos que s'han generat durant el desenvolupament de la tesi. Per una banda, hi ha el codi, amb el qual es poden extreure el conjunt de features tal i com es descriu a la tesi, per altre banda, hi ha també tots els datasets que s'han creat i que s'utilitzen per a tots els experiments. Utilitzant el codi, les eines externes corresponents i els datasets, es poden emular tots els experiments descrits.The zip file contains every resource that has been generated during the development of the thesis. One of the folders contains the code that is used to extract the described feature set, the other one contains every dataset that has been compiled and used in every experiment. Using the code, the external tools mentioned in the experiments and the corpora, it is possible to repeat every experiment described in the thesis
MIC determination of free AmB and MFLB-AmB against liquid suspensions of <i>Paracoccidioides brasiliensis</i>.
No full textFungal burden as assessed by Colony Forming Units (CFU) in Paracoccidioides brasiliensis strain 18 (Pb18) (2 x 104 cells/mL) while incubated in RPMI 1640 medium at 37°C for 72 hours with AmB or MFLB-AmB, as a function of amphotericin B, or MFLB, as a function of iron concentration. After 72 hour incubation, cell suspensions were plated on BHI (brain heart infusion) media, supplemented with 4% (v/v) of horse serum, 5% (v/v) of the supernatant from the culture filtrate of isolate Pb192 and 40 mg/L gentamycin, and then incubated for five days at 37°C in a rotary shaker (220 rpm).(AmB = amphotericin B, MFLB-AmB = nanosized magnetite surface-functionalized with a bilayer of lauric acid conjugated with amphotericin B, MFLB = nanosized magnetite surface-functionalized with a bilayer of lauric acid).</p
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