1,721,507 research outputs found
Editorial: Where do we come from and where do we go? Moving forward towards new goals for the journal
No full textTherapeutic approach to intrusive luxation injuries in primary dentition: a clinical follow up study
No full textAim This paper reports the results of a fifteen-years study carried out at the Dentoalveolar trauma study Centre of the University of Cagliari Dental Department, on treatment modes utilised for the recovery of periodontal injuries in primary dentition .Materials and Methods The data referred to dental injuries were collected and recovered according to Andreasen's dental trauma Classification: clinical Signs and symptoms, patient's age and gender, lesion site.The Authors focused their attention on intrusive luxation in pimary dentition, which are a very frequent trauma in children between 1-4 years of age. Results This careful conservative approach allowed the repositioning of about 60% of the 85 intruded teeth examined.It substantially reduced the number and severity of undesiderable sequelae, both local( enamel-hypoplasia) and occlusal(tooth retention), so that only in 25 % of the followed-up cases damages of the successor tooth were found in the permanent dentition.Conclusion The author conclude their study emphasizing that all those involved in paediatric Dentistry must be familiar with periodontal injuries and trained in their treatment, particularly as regard intrusive luxation in primary dentition
Heterologous Leader Sequences in Fusion Constructs Enhance Expression of Geranyl Diphosphate Synthase and Yield of β-Phellandrene Production in Cyanobacteria ( Synechocystis)
No full textFusion constructs as protein overexpression vectors proved to be critical in the heterologous expression of terpene synthases in cyanobacteria. The concept was recently applied to the heterologous overexpression of the beta-phellandrene synthase (beta-PHLS) from plants, fused to the highly expressed endogenous cpcB gene encoding the beta-subunit of phycocyanin. Overexpressed CpcB*PHLS fusion proteins enhanced the heterologous yield of C10H16 beta-phellandrene hydrocarbons production in Synechocystis. This work extended the concept of fusion constructs as protein overexpression vectors by showing that highly expressed heterologous genes could also serve as leader sequences for protein overexpression in cyanobacteria. Examined are the kanamycin nptI and chloramphenicol cmR resistance cassettes, both of which are overexpressed in Synechocystis. Evidence showed a dual purpose of the nptl gene, as a leader sequence fused to a heterologous geranyl-diphosphate synthase (GPPS), promoting its expression, while at the same time serving as a selectable marker for the screening of transformants. The work further showed that enhanced GPPS expression increased the yield of beta-phellandrene in Synechocystis transformants harboring the beta-PHLS gene. Moreover, the research evaluated the expression efficacy of a DNA fragment comprising 87 nucleotides from the S' end of the cmR gene in fusion with the GPPS gene. This short fusion construct substantially increased the intracellular geranyl-diphosphate synthase level, suggesting that "short-stretch" cmR leader sequences can be used to drive a higher expression level of heterologous biosynthetic genes, while avoiding undesirable internal recombinations, as these sequences are shorter than the threshold of 200 bp, commonly assumed to be the threshold of high efficiency recombinations
Ecocentrico: Alessandro Melis intervista Stefan Tischer
No full textIntervista di Stefan Tischer sulle grande tematiche dell'architettura del paesaggio nei suoi progett
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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