2,085 research outputs found
The Reproducibility of Lists of Differentially Expressed Genes in Microarray Studies
No full textReproducibility is a fundamental requirement in scientific experiments and clinical contexts. Recent publications raise concerns about the reliability of microarray technology because of the apparent lack of agreement between lists of differentially expressed genes (DEGs). In this study we demonstrate that (1) such discordance may stem from ranking and selecting DEGs solely by statistical significance (P) derived from widely used simple t-tests; (2) when fold change (FC) is used as the ranking criterion, the lists become much more reproducible, especially when fewer genes are selected; and (3) the instability of short DEG lists based on P cutoffs is an expected mathematical consequence of the high variability of the t-values. We recommend the use of FC ranking plus a non-stringent P cutoff as a baseline practice in order to generate more reproducible DEG lists. The FC criterion enhances reproducibility while the P criterion balances sensitivity and specificity
Le Corbeau et le Renard: 99 versions réinventées: Style d'exercices
No full textThis book represents a fascinating effort. At first I thought it was going to be a collection of various parodies of La Fontaine's FC. It is not that, but rather a creative presentation of some 97 different versions of La Fontaine created by the author. The first two versions are a French translation of the Greek prose of the most original FC text in the Aesopic manuscripts and then La Fontaine's verse. Thereafter the fun begins! The imagination and the execution of this project are both remarkable. Let me offer a sample of five approaches: (1) an acrostic, so that the opening letters of each verse spell "M Jean La Fontaine"; (2) a prose version after the manner of Balzac; (3) a scientific version needing more than a full page; (4) a tourist guide version; and (5) a rhyming version in English. T of C at the end. 136 pages. 5¼" x 8". Printed upon demand.Language note: FrenchNicolas Mille
FC Omniworld
No full textHet maken van een stedenbouwkundig plan voor de locatie Almere Poort in Almere. Hierbij is het programma van de Gemeente, zoals gespecificeerd voor de locatie, overgenomen. De kern van het nieuwe plan is een centrale (groene) as waaraan alle centrumfuncties voor deze wijk worden gerealiseerd. Deze nieuwe as zorgt, door de combinatie van functies met verschillende gebruikstijden, voor een continue drukte op deze centrale as. Aan een van de eindes van de as ligt een verhoogd maaiveld. Dit maaiveld gaat over het spoortalud zodat men het spoor over kan steken zonder tunnel of spoorwegovergang. In dit talud worden diverse functies gehuisvest welke normaal dichte dozen zijn (zoals parkeren, expo ruimten en sportzalen). Deze functies met voornaleijk dichte gevels worden omkleed met kleinschalige functies om continue activiteit bij het verhoogde maaiveld te genereren en om interessante gevelbeelden te hebben (i.p.v. dichte wanden van grootschalige functies). In het verhoogde maaiveld ligt verdiept het nieuwe stadion voor FC Omniworld. Dit stadion wordt zo opgenomen in een natuurlijke en groene omgeving. De dakconstructie bestaat uit houten spanten en een semi-transparant doek om zo goed mogelijk aan te sluiten op het park bovenop het nieuwe maaiveld. Bezoekers komen ook via het park binnen. Op dit niveau kan men rondlopen om versnaperingen te kopen en vervolgens af te dalen naar de tribunes. Het stadion is in de toekomst uit te breiden door nieuwe tribunes te positioneren boven de promenade van het stadion en onder het dak. Hiervoor zijn geen aanpassingen vereist. Tevens is het stadion multifuntioneel te gebruiken door verplaatsbare tribunedelen.Architectur
Plant expression of cocaine hydrolase-Fc fusion protein for treatment of cocaine abuse
No full textBACKGROUND: A recently reported cocaine hydrolase (CocH3) fused with fragment crystallizable (Fc) region of human immunoglobulin G1, denoted as CocH3-Fc, is known as a promising therapeutic candidate for the treatment of cocaine overdose and addiction. A challenge for practical therapeutic use of this enzyme exists in the large-scale protein production and, therefore, it is interesting to identify a low-cost and feasible, sustainable source of CocH3-Fc production.
RESULTS: CocH3-Fc was transiently expressed in plant Nicotiana benthamiana leaves. The plant-expressed protein, denoted as pCocH3-Fc, was as active as that expressed in mammalian cells both in vitro and in vivo. However, compared to the mammalian-cell expressed CocH3-Fc protein, pCocH3-Fc had a shorter biological half-life, probably due to the lack of protein sialylation in plant. Nevertheless, the in vivo half-life was significantly extended upon the PEGylation of pCocH3-Fc. The Fc fusion did not prolong the biological half-life of the plant-expressed enzyme pCocH3-Fc, but increased the yield of the enzyme expression in the plant under the same experimental conditions.
CONCLUSIONS: It is feasible to express pCocH3-Fc in plants. Further studies on the pCocH3-Fc production in plants should focus on the development of vectors with additional genes/promoters for the complete protein sialylation and for a better yield
Stable Foliage Cluster (FC), a Basic Unit of the Crown Structure of Tree Species and Its Application to Modeling of Tree and Forest Structure: Configuration of the FC Model
No full textThe "foliage cluster (FC) model" shows that the size (the amount of leaves, number of yearly shoots and total twig length), turnover time, and branching (i.e., forking) structure of a twig becomes stable in the top of the crown in an oak species by forming a "stable FC" [1]. A stable FC can be treated as a basic component of the crown structure and as an alternative to individual leaf or current-year shoot. The FC model is composed of several equations approximating the branching structure of twigs. Differences in size and branching morphology among twigs are represented by differences in values of the parameters of the equations. We described details of our analyses of data taken from real twigs and applied to the equations implemented in the FC model
Financial analysis of FC SLOVAN Liberec
No full textThesis "Financial analysis of FC Slovan Liberec" focuses especially on reviewing financial position and efficiency of this football club in four year period. The first theoretical part is complemented with practical calculations in the second part. In the second part author focuses on horizontal analysis, vertical analysis and other models of financial analysis
Cuidando do recém-nascido em UTIN: convivendo com a fragilidade do viver/sobreviver à luz da complexidade
No full textDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências da Saúde, Programa de Pós-Graduação em Enfermagem, Florianópolis, 2009.Este estudo trata da elaboração de um modelo que contempla o fenômeno do cuidado em Unidade de Terapia Intensiva Neonatal a partir da compreensão do ser e fazer enquanto enfermeiro neste sistema complexo. Tal compreensão aborda as transformações ocorridas desde a criação das UTINs até a atualidade, vislumbrando sua busca pela integralidade ao oferecer um cuidado sensível e compartilhado. O referencial metodológico da Teoria Fundamentada nos Dados (Grounded theory) associado ao Paradigma da Complexidade de Edgar Morin foi utilizado na análise e na construção do Modelo Teórico: CUIDANDO DO RECÉM-NASCIDO EM UTIN: Convivendo com a fragilidade do viver/sobreviver à luz da complexidade. Participaram da pesquisa 11 sujeitos, entre enfermeiros e mães com bebês internados na UTIN. Utilizou-se a entrevista aberta para coleta dos dados. A análise comparativa dos dados subsidiou o processo de codificação, amostragem, saturação teórica, ordenação e integração dos dados. Os dados foram inseridos no software NVIVO, dando seqüência na organização e agrupamento dos mesmos. A organização dos códigos foi feita de acordo com suas propriedades e, então, agrupados em códigos preliminares, subcategorias e categorias. Através das inter-relações entre categorias, emergente dos dados coletados, identificou-se a categoria central, eixo norteador do Modelo Teórico, e integradora das categorias analíticas denominada: Convivendo com a fragilidade do viver/sobreviver: cuidado altamente complexo, sensível, singular e compartilhado em torno da qual giram as demais categorias: Buscando conhecimentos e competências; Gerenciando o cuidado na UTIN e Vivenciando as singularidades na UTIN. Neste contexto, o cuidado em UTIN se fortalece como uma das áreas da Enfermagem em constante desenvolvimento, visando conciliar os avanços tecnológicos importantes para o sobreviver do bebê prematuro com abordagens que valorizam as inter-relações em seu quotidiano, de modo sistêmico. Busca atuar nas diversas esferas do cuidado complexo em saúde, desde os serviços de apoio da instituição hospitalar e seus gestores até a academia, visando evitar atuações compartimentadas e isoladas, integrando e aplicando conhecimentos científicos, com ganhos para a prática profissional. Oferecer suporte tecnológico já não basta. A pesquisa ressalta que é necessário exercitar as potencialidades já inatas dos profissionais de enfermagem e caminhar para o encontro de outras, um verdadeiro convite a novas percepções de cuidar do neonato, sua família e os membros deste sistema complexo, numa constante inquietação e adaptação para as demandas que surgem. Assim, as indagações norteadoras desta pesquisa convidam para a ampliação de novos horizontes de investigação, de forma que as possibilidades de estudos indicadas permitam o aprofundamento no âmbito das organizações de saúde e da academia.This study deals with the elaboration of a model which contemplates the phenomenon of care in a Neonatal Intensive Care Unit (NICU), based on the comprehension of the human being and human actor as a nurse in this complex system. Such comprehension encompasses the transformations which have occurred since the creation of the NICUs, discerning its search for the entirety to offer sensible and shared care. The methodological reference of the Grounded Theory associated with Edgar Morins Complexity Theory was utilized in analysis and the construction of the Theoretical Model: CARING FOR THE NEWBORN IN THE NICU: Living with the fragility of living/surviving in the light of complexity. The sample was composed of 11 subjects, including nurses and mothers with newborns in the NICU. Open interviews were used in order to collect data. Comparative data analysis subsidized the data codification, sampling, theoretical saturation, ordering, and integration processes. Data was inserted into NVIVO software, providing a sequence in data organization and grouping. Organizing the codes was done according to their properties and thus, grouped in preliminary codes, subcategories, and categories. Through the relationships in these categories which emerged from the data collected, the central category, the guiding axis of the Theoretical Model and the integrating factor of the analytical categories was identified, denominated: Living with the fragility of living/surviving: highly complex, sensitive, singular, and shared care around which are the following categories: Seeking knowledge and competencies; Managing NICU care; and Living the singularities of the NICU. In this context, NICU care is strengthened as one of the Nursing areas in constant development, seeking to conciliate important technological advances towards premature newborn survival with the approaches which systematically value day-to-day relationships. It seeks to enact in the diverse spheres of complex health care, from the support services of the hospital institution and its management to academia, searching to avoid fragmented and isolated care through integrating and applying scientific knowledge with gains for professional practice. Offering technological support is no longer enough. Research highlights that it is necessary to exercise already innate potential in nursing professionals and work towards finding others, a true invitation towards new perceptions for care for the newborn, his/her family, and the members of this complex system in a constant unrest and adaptation of demands which may arise. Thus, the guiding questions of this study invite towards the amplification of new investigative horizons, in such a manner that the possibilities for suggested studies permit more profound in the realm of health care and academic organizations
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