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การคัดเลือกบาซิลลัสที่ผลิตแบคเทอริโอซินต้านเชื้อก่อโรคปลาจากอาหารหมักและการศึกษาคุณสมบัติเบื้องต้นของแบคเทอริโอซิน
Get PDFScreening of Bacteriocin–Producing Bacillus against Fish Pathogens from Fermented Foods and Preliminary Characterization of the Bacteriocin
Siriporn Tipsing, Saowanit Tongpim and Ratchanu Meidong
รับบทความ: 27 พฤศจิกายน 2561; แก้ไขบทความ: 23 มีนาคม 2562; ยอมรับตีพิมพ์: 30 เมษายน 2562
บทคัดย่อ
งานวิจัยนี้มีวัตถุประสงค์เพื่อคัดเลือกและศึกษาคุณสมบัติของแบคเทอริโอซินจากบาซิลลัสที่แยกจากอาหารหมักของไทย โดยบาซิลลัส 258 ไอโซเลตแยกมาจากตัวอย่างอาหารหมักจำนวน 62 ตัวอย่างและนำไปศึกษาความสามารถในการผลิตแบคเทอริโอซินต้านเชื้อก่อโรคปลาด้วยวิธี agar well diffusion assay ผลการวิจัยพบว่า ไอโซเลต BB60a ซึ่งแยกมาจากผลิตภัณฑ์ปลาส้ม มีประสิทธิภาพสูงสุดในการยับยั้งเชื้อก่อโรคปลาที่เป็นแบคทีเรียแกรมบวก (Streptococcus agalactiae) และแบคทีเรียแกรมลบ (Aeromonas hydrophila) เมื่อจำแนกระบุชนิดของไอโซเลต BB60a ด้วยลักษณะทางสัณฐานวิทยาร่วมกับลำดับ 16S rDNA พบว่า มีความใกล้เคียงกับ Bacillus megaterium ร้อยละ 99.83 นอกจากนี้ยังศึกษาผลของแหล่งไนโตรเจนต่อ B. megaterium BB60a ในการผลิตสารแบคเทอริโอซิน พบว่า yeast extract ความเข้มข้น 12 กรัมต่อลิตรที่เป็นองค์ประกอบของอาหารเลี้ยงเชื้อช่วยส่งเสริมให้เชื้อผลิตแบคเทอริโอซินได้สูงสุด (p < 0.05) จากการศึกษาคุณสมบัติเบื้องต้นของสารแบคเทอริโอซินพบว่ามีความเสถียรในช่วง pH 4–10 และในช่วงอุณหภูมิ 40–70ºC และพบ ว่าสารนี้ถูกทำลายได้ด้วยเอนไซม์ trypsin และ proteinase K แต่ทนต่อเอนไซม์ α-amylase และ lipase จากผลการศึกษานี้สรุปได้ว่าแบคเทอริโอซินจาก B. megatherium BB60a ออกฤทธิ์การยับยั้งเป็นแบบ broad spectrum ทนสภาพกรด–เบสได้ในช่วงกว้าง และทนต่อความร้อน จึงเหมาะสมที่จะนำไปประยุกต์ใช้ในการเลี้ยงปลาเพื่อยับยั้งการติดเชื้อก่อโรคปลาคำสำคัญ: บาซิลลัส แบคเทอริโอซิน เชื้อก่อโรคปลา
Abstract
The aims of this study were to screen and preliminarily characterize the properties of bacteriocin produced by Bacillus isolated from Thai fermented foods. In this study, 258 isolates of Bacillus were obtained from 62 samples of Thai fermented foods and screened for their bacteriocin against fish pathogens. Antimicrobial activity of bacteriocin was evaluated using an agar well diffusion assay. Isolate BB60a derived from fermented fish product showed the highest antimicrobial activities against fish pathogens which were both Gram positive (Streptococcus agalactiae) and Gram negative (Aeromonas hydrophila) bacteria. Bacterial identification based on cell morphology and 16S rDNA sequence revealed that the isolate BB60a was closely related to Bacillus megaterium (99.83% similarity). The effect of nitrogen sources on bacteriocin production was investigated. B. megaterium BB60a produced the highest bacteriocin when cultivated in the medium containing 12 g/L yeast extract. This bacteriocin showed stability over a wide range of pH (4–10) and temperatures (40–70ºC). It was denatured completely by trypsin and proteinase K, but not by α-amylase and lipase. From this study, it can be concluded that B. megaterium BB60a produces a broad-spectrum bacteriocin that tolerates acidic, alkaline and high temperature conditions and, therefore, it has the potential application in fish culture to prevent bacterial fish infection.
Keywords: Bacillus, Bacteriocin, Fish pathogen
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
Author Under Sail The Imagination of Jack London, 1893-1902
No full textIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Intro -- Title Page -- Copyright Page -- Dedication -- Contents -- Acknowledgments -- Introduction -- 1. Spirit Truth -- 2. From Absorption to Theatricality and Back Again -- 3. "I Will Build a New Present" -- 4. Sons as Authors -- 5. Fathers as Publishers -- 6. The Daughter as Author -- 7. Lovers as Authors -- 8. At Sea with the Family -- 9. Yellow News, Yellow Stories -- 10. The Return Home -- Notes -- Bibliography -- Index -- About Jay WilliamsIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
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