3 research outputs found
Histopathological Effect of Extracellular Products of Clinical Isolates of Pseudomonas aeruginosa on Mice Lungs.
No full textThe bacterial secretions into the growth medium that are produced as a result of the dynamics of growth and death of bacteria such as proteins, nucleic acids, and polysaccharides stimulate inflammatory response. The effect of extracellular bacterial secretions that are released by bacteria during growth on inflammatory response as well as the body\u27s tissues is not clear. The current study aims to highlight the effect of sterile bacterial secretion (IBS) of clinical isolate Pseudomonas aeruginosa on lung tissue. Here, P. aeruginosa was isolated from urine and grown on Luria-Bertani (LB) broth for a day, and IBSs were collected after filtration using a Millipore filter. These materials were administered to the experimental mice intranasally (i.n.). The tissue sections of the mouse lungs and the number of leukocytes in the bronchoalveolar lavage (BAL) were examined. The histological sections showed an increase in the leukocyte infiltration into the alveoli cavity and walls was seen as early as 4 h post-installation, and this continued until the second day. However, at 72 post instillation, it was found that the lung tissue had almost completely recovered and returned to its normal shape. The study also showed an increase in the number of Leukocytes number in BAL of mice that were given the IBS as early as 4 h post instillation this increase continued until 72 h. The results showed the effect of IBS in stimulating a safe inflammatory response in the lungs of mice, and this could help in the possibility of using these materials in the future to increase the immunostimulation of weak immunostimulatory antigens.
 
Seroprevalence of hepatitis E virus infection in acute leukemia patients with allogeneic transplantation
No full textAim: Hepatitis E Virus (HEV) infection is an emerging threat in allogeneic Hematopoietic Stem Cell Transplant (allo-HSCT) recipients. The aim of this study was to determine the prevalence of HEV infection in patients with acute leukemia before and after the transplantation.Material and Methods: The study was carried out with the serum samples taken before and after the transplantation from patients (n=42) with acute leukemia, who were 4 months to 64 years of age and who had allogenic transplant at Gazi University Hospital. All samples were tested in terms of HEV-specific anti-IgG and IgM positive value by using ELISA kit.Results: While 1 (2.3%) of the samples that were included in the study was positive anti-HEV IgG before transplantation, it was found negative after the transplantation (58th month). Positive anti-HEV IgM was not found before and after the transplantation in the collected samples. The average age of the seronegative group was determined as 30.8 ± 33.1 for male and female, respectively. There was no statistically significant difference in terms of anti-HEV IgM positivity and anti-HEV IgG positivity compared with seronegative cases of seropositive cases.Conclusions: This study was conducted to determine the prevalence of HEV infection in allogeneic stem cell patients. With this study, the incidence and importance of HEV can be determined in these patients before and after transplantation. This study can also create an important epidemiological data for our country
Resistance Patterns of Multi-Drug Gram-Negative Isolates to Carbapenems: An Emerging Problem
No full textObjective: Infections caused by multidrug-resistant (MDR) Gram-negative bacteria and resistance to carbapenems constitute a major public health problem worldwide, due to limited treatment options and high mortality rates. In this study, we aimed to determine the resistance profiles of MDR Gram-negative bacilli isolated from clinical samples to carbapenems. Material and Method: Specimens, from which MDR Gram-negative bacteria were isolated, were cultured onto 5% sheep blood and EMB agar, and VITEK 2 automated system (bio M & eacute;rieux, France) was used for identification and antimicrobial susceptibility testing. Data were analyzed using chi-square and Fisher's exact tests. Results: 1072 MDR bacterial strains were isolated from the specimens of 272 patients, with the majority of transtracheal aspirates (64%) sent predominantly from palliative care (49.8%) and intensive care (42%) units. The leading pathogens were Pseudomonas aeruginosa (42,1%), Acinetobacter baumannii (31,2%), and Klebsiella spp (22,5%). The highest resistance rate among carbapenems was detected to meropenem (91,6%), followed by imipenem (44,3%) and ertapenem (25%). While ertapenem resistance was significantly lower in P. aeruginosa strains (p<0,01), meropenem resistance was significantly higher in A. baumannii strains when compared to other antibiotics. Conclusion: Since Gram-negative MDR bacteria continue to spread rapidly, monitoring resistance profiles through active hospital surveillance is crucial to determine the appropriate treatment. The data obtained in this study once again highlight the importance of resistance to carbapenems and is considered to contribute to epidemiological data.Emerging Sources Citation Inde
