1,872 research outputs found
Ask questions, get sales : close the deak and create long-term relationships / Stephan Schiffman.
No full textIncludes index.v, 168 pages ;In Ask Questions, Get Sales, the author and sales guru Stephan Schiffman helps readers boost their careers to the gold-medal level by teaching them how to strengthen their questioning skills during the sales process. The premise is simple yet effective: In order to be successful, salespeople need to change their mindset from "need-orientated" to "do-orientated". The message of the book centers around six core "do" questions: What do you do? How do you do it? When and where do you do it? Why do you do it that way? Who do you do it with? How can we help you do it better? With this indispensable guide in their briefcase, salespeople will have information at the ready to score big sales over the short term and the long term
Unemployment Benefits and Unemployment Rates of Low-Skilled and Elder Workers in West Germany: A Search Equilibrium Approach
No full textApproach Author & abstract Download 16 References 1 Citations Related works & more Corrections Author Listed: Launov, Andrey ([email protected]) (University of Kent) Wolff, Joachim ([email protected]) (Institute for Employment Research (IAB), Nuremberg) Klasen, Stephan ([email protected]) (University of Göttingen) Registered: Stephan Klasen Abstract In this paper we investigate whether the extension of the entitlement to unemployment benefits in the mid 80s can explain the increase in the unemployment rates of unskilled and elder workers in western Germany. To answer this question we estimate a version of the Burdett-Mortensen search equilibrium model and analyze how workers’ search behaviour responded to these reforms. We try both nonparametric and fully-parametric estimation methods and identify the cases in which the nonparametric approach cannot be applied. We find that the entitlement reforms are largely responsible for the increase of unemployment among unskilled workers
Unemployment Benefits and Unemployment Rates of Low-Skilled and Elder Workers in West Germany: A Search Equilibrium Approach
No full textApproach Author & abstract Download 16 References 1 Citations Related works & more Corrections Author Listed: Launov, Andrey ([email protected]) (University of Kent) Wolff, Joachim ([email protected]) (Institute for Employment Research (IAB), Nuremberg) Klasen, Stephan ([email protected]) (University of Göttingen) Registered: Stephan Klasen Abstract In this paper we investigate whether the extension of the entitlement to unemployment benefits in the mid 80s can explain the increase in the unemployment rates of unskilled and elder workers in western Germany. To answer this question we estimate a version of the Burdett-Mortensen search equilibrium model and analyze how workers’ search behaviour responded to these reforms. We try both nonparametric and fully-parametric estimation methods and identify the cases in which the nonparametric approach cannot be applied. We find that the entitlement reforms are largely responsible for the increase of unemployment among unskilled workers
MALDI-MS tissue imaging identification of biliverdin reductase B overexpression in prostate cancer
No full textAbstract not availableJohannes Dominikus Pallua, Georg Schaefer, Christof Seifarth, Michael Becker, Stephan Meding, Sandra Rauser, Axel Walch, Michael Handler, Michael Netzer, Marina Popovscaia, Melanie Osl, Christian Baumgartner, Herbert Lindner, Leopold Kremser, Bettina Sarg, Georg Bartsch, Christian W. Huck, Günther K. Bonn, Helmut Klocke
Measuring Vulnerability to Poverty Using Long-Term Panel Data
No full textMeasuring Vulnerability to Poverty Using Long-Term Panel Data Author & abstract Download & other version 16 References 4 Citations Related works & more Corrections Author Listed: Katja Landau (Georg-August-University Göttingen) Stephan Klasen (Georg-August-University Göttingen) Walter Zucchini (Georg-August-University Göttingen) Registered: Stephan Klasen Abstract We investigate the accuracy of ex ante assessments of vulnerability to income poverty using cross-sectional data and panel data. We use long-term panel data from Germany and apply di fferent regression models, based on household covariates and previous-year equivalence income, to classify a household as vulnerable or not. Predictive performance is assessed using the Receiver Operating Characteristics (ROC), which takes account of false positive as well as true positive rates. Estimates based on cross-sectional data are much less accurate than those based on panel data, but for Germany, the accuracy of vulnerability predictions is limited even when panel data are used. In part this low accuracy is due to low poverty incidence and high mobility in and out of poverty
Measuring Vulnerability to Poverty Using Long-Term Panel Data
No full textMeasuring Vulnerability to Poverty Using Long-Term Panel Data Author & abstract Download & other version 16 References 4 Citations Related works & more Corrections Author Listed: Katja Landau (Georg-August-University Göttingen) Stephan Klasen (Georg-August-University Göttingen) Walter Zucchini (Georg-August-University Göttingen) Registered: Stephan Klasen Abstract We investigate the accuracy of ex ante assessments of vulnerability to income poverty using cross-sectional data and panel data. We use long-term panel data from Germany and apply di fferent regression models, based on household covariates and previous-year equivalence income, to classify a household as vulnerable or not. Predictive performance is assessed using the Receiver Operating Characteristics (ROC), which takes account of false positive as well as true positive rates. Estimates based on cross-sectional data are much less accurate than those based on panel data, but for Germany, the accuracy of vulnerability predictions is limited even when panel data are used. In part this low accuracy is due to low poverty incidence and high mobility in and out of poverty
Evaluation of in-store processes related to returnable packaging services offered in grocery stores - the store management perspective
No full textAuthor Stephan LehnerMasterarbeit Universität Linz 202
Evaluation of in-store processes related to returnable packaging services offered in grocery stores - the store management perspective
No full textAuthor Stephan LehnerMasterarbeit Universität Linz 202
Matrix-assisted laser desorption/ionization imaging protocol for in situ characterization of tryptic peptide identity and distribution in formalin-fixed tissue
No full textLink to a related website: https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/rcm.6488, Open Access via UnpaywallRATIONALE: Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry provides the means tomap the in situ distribution of tryptic peptides in formalin-fixed clinical tissue samples. The ability to analyze clinicalsamples is of great importance to further developments in the imaging field. However, there is a requirement in this fieldof research for additional methods describing the characterization of tryptic peptides by MALDI imaging.
METHODS AND RESULTS: This protocol gives highly detailed instructions, with examples, for (1) successfully performingtryptic peptide MALDI imaging on formalin-fixed cancer tissue using a MALDI-TOF/TOF MS instrument, (2) tentativelygenerating identifications through nLC/MS/MS, and (3) validating these identifications by in situ MS/MS of peptides ofinterest.
CONCLUSIONS: This protocol provides a detailed and straightforward description of the methods required for groupsnew to MALDI imaging to begin analysis of formalin-fixed clinical samples.Ove J. R. Gustafsson, James S. Eddes, Stephan Meding, Shaun R. McColl, Martin K. Oehler and Peter Hoffman
Internal calibrants allow high accuracy peptide matching between MALDI imaging MS and LC-MS/MS
No full textOne of the important challenges for MALDI imaging mass spectrometry (MALDI-IMS) is theunambiguous identification of measured analytes. One way to do this is to match trypticpeptide MALDI-IMS m/z values with LC-MS/MS identified m/z values. Matching using currentMALDI-TOF/TOF MS instruments is difficult due to the variability of in situ time-of-flight (TOF)m/z measurements. This variability is currently addressed using external calibration, whichlimits achievable mass accuracy for MALDI-IMS and makes it difficult to match these data todownstream LC-MS/MS results. To overcome this challenge, the work presented here details amethod for internally calibrating data sets generated from tryptic peptide MALDI-IMS onformalin-fixed paraffin-embedded sections of ovarian cancer. By calibrating all spectra tointernal peak features the m/z error for matches made between MALDI-IMS m/z values and LCMS/MSidentified peptide m/z values was significantly reduced. This improvement wasconfirmed by follow up matching of LC-MS/MS spectra to in situ MS/MS spectra from the samem/z peak features. The sum of the data presented here indicates that internal calibrantsshould be a standard component of tryptic peptide MALDI-IMS experiments.This article is part of a Special Issue entitled: Imaging Mass Spectrometry: A User’s Guide toa New Technique for Biological and Biomedical Research.Johan O.R. Gustafsson, James S. Eddes, Stephan Meding, Tomas Koudelka, Martin K. Oehler, Shaun R. McColl and Peter Hoffman
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