11,215 research outputs found

    Isolation and properties of a liver mitochondrial precursor protein to aspartate aminotransferase expressed in Escherichia coli.

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    The precursor to rat liver mitochondrial aspartate aminotransferase has been expressed in Escherichia coli JM105 using the pKK233-2 expression vector. This mammalian natural precursor has been isolated as a soluble dimeric protein. The amino-terminal sequence and the amino acid composition of the isolated protein correspond to those predicted from the inserted cDNA (Mattingly, J. R., Jr., Rodriguez-Berrocal, F. J., Gordon, J., Iriarte, A., and Martinez-Carrion, M. (1987) Biochem. Biophys. Res. Commun. 149, 859-865). The isolated precursor contains bound pyridoxal phosphate and shows catalytic activity with a specific activity equal to that of the mature form of the enzyme. This precursor can also be processed by mitochondria into a form with the sodium dodecyl sulfate-polyacrylamide gel electrophoresis mobility of mature enzyme. The isolation of this precursor as a stable and catalytically active entity indicates that the presequence peptide does not necessarily interfere with much of the folding and basic structural properties of the mature protein component

    Letter from T.H. Hayes, Jr. to Attorney Henry M. Beaty Jr

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    A letter of recommendation for Russell B. Sugarmon, Jr. to be admitted to the bar in Memphis and Shelby County. The author commends his ability, character, and family background

    2015 Kansas Performance Tests with Soybean Varieties

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    Soybean performance tests are conductd each year to provide information on the relative performance of new and established varieties and brands at several locations in Kansas. Main Station, Manhattan: Jane Lingenfelser, Assistant Agronomist; William T. Schapaugh, Jr., Professor (Senior Author); Brent Christenson, Research Assistant; Cheyenne Stephens, Research Assistant; Research Centers: Patrick Evans, Colby; Lonnie Mengarelli, Parsons; Monty Spangler, Garden City; Josh Coltrain, Crawford County Extension; Experiment Fields: Eric Adee, Topeka; Gary Cramer, Hutchinson; Andrew Esser, Scandia; James Kimball, Ottawa; Cooperators: Vernon Egbert, McCune; Lance Rezac, Onaga; Dale Roberds, Pittsburg; Clayton Short, Assaria

    Alf Mapp, Jr., 13th Annual ODU Literary Festival

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    An Eminent Scholar at Old Dominion University, Alf Mapp, Jr., is the author of six books, most recently Thomas Jefferson: A Strange Case of Mistaken Identity, cited as one of the Forty Best Books of 1987. He has written many other books and is the author of more than 800 articles in the New York Times and other metropolitan newspapers, scholarly journals, and popular magazines

    Burdock Burr Breese, Jr.

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    Burtis Burr Breese, Jr. (1905-1998) was the son of the psychologist and author Burtis Burr Breese, Sr. (1868-1939). Burtis Burr Breese, Jr. received his undergraduate degree from Princeton University (1927) and his medical degree from Harvard Medical School (1931). He interned at Johns Hopkins Hospital (1931/32) and the New York Hospital (1932/33). In 1933/34 Breese was made assistant resident pediatrician at Strong Memorial Hospital and assistant in pediatrics at the University of Rochester School of Medicine & Dentistry (URSMD). He was appointed instructor in pediatrics in 1934. Between 1935 and 1952 held appointments in the Dept. of Pediatrics, during which period he also served in the U.S. Navy (1942-46). During his military service Breese became interested in meningococcal and streptococcal infections. He was appointed assistant professor of pediatrics at URSMD in 1952 and clinical associate professor in 1958. Breese was one of the co-founders of the Elmwood Pediatric Group (EPG), using his practice to conduct research on childhood infections. He was the first to culture strep throat in an office setting by constructing an incubator from parts of chicken hatching equipment. Breese and his partner at EPG, Frank A. Disney, M.D., received the Practitioner's Excellence Award from the American Academy of Pediatrics. Breese was the author of at least 69 articles and co-authored with his daughter Caroline Breese Hall the monograph Beta Hemolytic Streptococcal Diseases (Boston, 1978)

    Poem titled "Home" with a recorded reading by the author, Charles E. Broadwater Jr.

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    Charles E. Broadwater Jr. is one of the 2007 recipients of Virginia Tech's Alumni Distinguished Service Award, which recognizes outstanding service to the university and the Alumni Association. Broadwater graduated from Virginia Tech with a bachelor's degree in 1972

    Henry Adams, Jr. letter to father, February 5, 1952

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    This letter was written by Henry Adams Jr. to his father, Henry Adams, expressing his feelings and experiences during his time in the army. Junior, as he was called in the family, had been posted to Alaska after his basic Army training, and assigned to an otherwise all-white company. In this three-page letter written on decorative notepaper, Junior writes about his army experiences, and says that he feels like Jackie Robinson, a test case for integrated units in the military. The United States Army was not integrated during World War II; African Americans and whites served in separate units. Harry Truman issued an executive order intended to end segregation in the Army in January of 1948, and letters such as this indicate that the executive order took some time to become fully effective

    January 22, 1985 letter from Ralph A. Patterson, Jr., Diamond Shamrock Thermal Power Company, to Takeshi Yoshihara, DPED, Energy Division

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    The author of the letter is named as Ralph A. Patterson Jr., but "crn" actually wrote the letter (per "RAP/crn"). The original letter included attachments (not included in the digital file)

    Memorandum to Colonel W. L. Magill, Jr. Provost Marshal and Director of Evacuation

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    Memorandum to the Colonel W.L. Magill Jr., Provost Marshal and Director of Evacuation presumably from a committee with the following members: Galen M. Fisher, Gordon Chapman, C. A. Richardson, and F. H. Smith. The memo includes the following subtitles: General Purpose and General Considerations.The Bishop James Chamberlain Baker Collection includes letters, documents, and articles about Japanese Americans during World War II. Subjects in the collection include Japanese Americans mass removal, Pearl Harbor and the aftermath, religion, and support from the non-Japanese American community. The collection was digitized and made accessible online by CSUDH Gerth Archives and Special Collections
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