306 research outputs found
Somatic gene therapy for cancer. The utility of transferrinfection in generating ‘tumor vaccines’
No full textThe last few years have seen the development of a branch of somatic gene therapy which aims at strengthening the immune surveillance of the body, leading to eradication of disseminated cancer tumor cells and occult micrometastases after surgical removal of the primary tumor. Such a tumor vaccination protocol calls for cultivation of the primary tumor tissue and the insertion of one of three types of genes into the isolated cultured tumor cells followed by irradiation of the transfected or transduced cells to render them incapable of further proliferation. The cells so treated constitute the ‘tumor vaccine’. A review of the literature suggests that for mouse models, in the initial period after inoculation, rejection of the tumor cells is usually effected by non-T-cell immunity, whereas the long-term systemic immune response is based on cytotoxic T-cells. High expression of the gene inserted into the tumor cells may be critical for the success of the vaccination procedure. Examples are given which indicate that transferrinfection, a procedure to introduce genes by adenovirus-augmented receptor-mediated endocytosis, meets some important prerequisites for successful application of this type of gene therapy
mlcotten13/Measles_primers
No full textMeasles primers for full genome sequencing of Measle virus genotype B3. These are version 1 primers used for the MSA deposition in June 2022
Structural Modification of the Flavanone Naringenin – Potential Impacts in Dietary Polyphenol Research
No full textEmerg Infect Dis
No full textWe describe a cluster of COVID-19 breakthrough infections after vaccination in Kyamulibwa, Kalungu District, Uganda. All but 1 infection were from SARS-CoV-2 Omicron strain BA.5.2.1. We identified 6 distinct genotypes by genome sequencing. Infections were mild, suggesting vaccination is not protective against infection but may limit disease severity.MC_PC_20011/MRC_/Medical Research CouncilUnited Kingdom/MC_PC_20001/MRC_/Medical Research CouncilUnited Kingdom/220977/Z/20/Z/WT_/Wellcome TrustUnited Kingdom/MC_PC_20010/MRC_/Medical Research CouncilUnited Kingdom/MC_EX_MR/L016273/1/MRC_/Medical Research CouncilUnited Kingdom
Measles whole-genome sequencing
No full textThis is an optimised protocol for PCR amplification of the Measles virus (MV) genome using specific primers designed for B3 genotype. The method generates cDNA amplicons suitable for whole genome sequencing using the MinION platform. The method covers the entire ca. 16,000 nt MV genome in 8 overlapping amplicons of about 2200 bp (see Figure below). Both the reverse transcription and PCR steps are performed in two separate reactions to avoid short overlapping PCR products.
The method has worked successfully with MV directly from oral fluid samples and with tissue culture passaged material. Primer sequences can be found at the following GitHub site: https://github.com/mlcotten13/Measles_primer
The adenovirus protease is required for virus entry into host cells
No full textWe have analyzed the mechanisms used by adenovirus to gain entry into the host cell. Using both virus infection and the ability of adenovirus particles to enhance polylysine/DNA uptake as a measure of virus entry, we have demonstrated that the adenovirus-encoded 23K protease is required for two functions in the infection process. A proteolytic processing of the capsid is required to generate a virus capsid that can increase membrane interactions at pH 5. We have found that forms of adenovirus capsid that have not undergone the processing reactions (immature capsids) are deficient in their ability to disrupt membranes at pH 5 and are unable to enhance the entry of polylysine/DNA complexes. A second role of the protease was revealed by experiments using inhibitors of the protease. Mature virus capsids lose their ability to enhance gene delivery and become noninfectious after exposure to inhibitors of the protease (1 μM N-ethylmaleimide, 100–300 μMcopper chloride, 1 μMMDL28170, or anti-protease antiserum), suggesting that the viral protease activity is required during the cellular entry process
Computer-Assisted Language Instruction: Needs and Means
No full textForeign language teachers are sometimes hesitant about using computers to aid their instruction either because the technology may not seem readily applicable to their courses, or it may seem overly time consuming to prepare the materials. In reality, computer software can be easily learned and used by instructors to support any type of teaching situation. In this paper, the author will discuss the various ways in which computers can be used to assist the instruction of English as a foreign language (EFL). Various practical uses for computer-assisted language instruction (CALI) will be explained, along with a detailed description of a project in which student-created vocabulary quizzes were uploaded to a teacher-made homepage accessible via the campus intranet.Foreign language teachers are sometimes hesitant about using computers to aid their instruction either because the technology may not seem readily applicable to their courses, or it may seem overly time consuming to prepare the materials. In reality, computer software can be easily learned and used by instructors to support any type of teaching situation. In this paper, the author will discuss the various ways in which computers can be used to assist the instruction of English as a foreign language (EFL). Various practical uses for computer-assisted language instruction (CALI) will be explained, along with a detailed description of a project in which student-created vocabulary quizzes were uploaded to a teacher-made homepage accessible via the campus intranet.departmental bulletin pape
The white doe : the fate of Virginia Dare : an Indian legend /
No full textIncludes brief history and photograph of Scuppernong (a variety of muscadine grape) vineyards on Roanoak Island, North Carolina.In verse.Spine dated 1904.Title-page in red and black.Includes bibliographical references (p. 81-89).Includes brief history and photograph of Scuppernong (a variety of muscadine grape) vineyards on Roanoak Island, North Carolina.Mode of access: Internet.Imperfect: front flyleaf lacking.ACQ: 35669; Janice B. Longone; Gift; 6/21/2001
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