35 research outputs found
SYNTHESIS OF MAGNETIC CROSS-LINKED ENZYME AGGREGATES WITH ETHANOL PRECIPITATION
No full textDiplomsko delo zajema pripravo magnetnih zamreženih encimskih skupkov (ali na kratko mCLEAs) iz encima celulaze. Naš namen je bil določiti optimalne pogoje za pripravo mCLEAs tako, da bosta učinkovitost in aktivnost imobilizacije zamreženih encimskih skupkov najvišja. V prvem delu diplomskega dela smo pripravili magnetne zamrežene encimske skupke s precipitacijo z etanolom. Pri tem smo spreminjali različne parametre (hitrost stresanja, čas stresanja, koncentracijo glutaraldehida in temperaturo), medtem ko je bil namen drugega dela diplomskega dela izpostavitev mCLEAs različnim temperaturam (23°C, 4°C, -15°C), ter ugotavljanje, kako se aktivnost mCLEAs pri različnih pogojih izpostavitve spreminja s časom.
Naši rezultati prikazujejo, da je bila aktivnost mCLEAs najvišja, ko smo si mCLEAs pripravili pri 10°C in uporabili 0,7 % koncetracijo glutaraldehida.
Pri izpostavitvi mCLEAs različnim temperaturam pa smo ugotovili, da aktivnost mCLEAs s časom najbolj upada kadar so bili mCLEAs izpostavljeni pri -15°C.The aim of this work was to prepare the magnetic cross-linked enzyme aggregates (or briefly mCLEAs) of the enzyme cellulase. Our goal was to optimize the preparation of mCLEAs so that the efficiency and activity of the immobilized cross-linked enzyme would be preserved. In the first part of the thesis, we used ethanol precipitation to prepare magnetic cross-linked enzyme aggregates. In this part we tested how different condition (agitation rate, the agitation time, the concentration of glutaraldehyde, and temperature) affect mCLEAS activity. In the second part of the thesis we tested how different temperatures that we used during mCLEAs preparation (23 ° C, 4 ° C, -15 ° C) affect the final mCLEAs activity.
Our results show that we obtain the highest final mCLEAs activity when we are working at 10°C and using 0.7% glutaraldehyde.
The highest decrease in mCLEAs activity we obtained when mCLEAs were exposed to -15 ° C
Synthesis and isolation of nanocellulose from bacteria Gluconacetobacter xylinus
No full textMagistrska naloga zajema izolacijo, sintezo ter karakterizacijo bakterijske nanoceluloze. V prvem delu magistrske naloge smo se osredotočili na pripravo rastnega medija in različnih produkcijskih gojišč ter gojenje bakterije Gluconacetobacter xylinus. V drugem delu smo najprej izolirali bakterijsko nanocelulozo (BNC) iz produkcijskega gojišča z kalcijevim karbonatom ter produkcijskega gojišča s citronsko kislino z obdelavo z natrijevim hidroksidom (NaOH). Na koncu smo oba vzorca BNC karakterizirali z različnimi analiznimi metodami. Z metodami FTIR, SEM in EDS smo dokazali, da sta oba vzorca vsebovala celulozo. Z metodo EDS/DLS pa smo dokazali, da je BNC pridobljena iz medija s citronsko kislino bolj temperaturno obstojna, prav tako se je v tem mediju sintetiziralo največ BNC.The master\u27s thesis involves the isolation, synthesis and characterization of bacterial nanocellulose. The first part of the master\u27s thesis is focused on the preparation of growth medium and various production media and the cultivation of the bacterium Gluconacetobacter xylinus. In the second part, the isolation of bacterial nanocellulose (BNC) from the calcium carbonate production medium, and the production medium with citric acid by treatment with sodium hydroxide (NaOH) was described. In the end, both BNC samples were characterized by different analytical methods. With the FTIR, SEM and EDX methods was demonstrated that both samples contained cellulose. However, with the EDX/DLS method was observed that BNC obtained from the medium with citric acid is more temperature resistant, and also more BNC have been produced in this medium
Participants in administrative proceeding and their legal status
No full textParticipants in administrative proceeding and their legal status Abstract This rigorous thesis deals with the participants in administrative proceedings and their legal status. The aim of the thesis is to describe the legal regulation of the participants in administrative proceedings and their selected procedural rights in the Czech Republic, to point out the deficits of the legal regulation and to present proposals de lege ferenda. The author used descriptive method and critical analysis in writing. The thesis also contains a comparison with foreign legislation. The author draws on Czech and foreign sources. The thesis contains the author's observations on the subject. The rigorous thesis is composed of the introduction, eight chapters and the end. In the introduction, the author states the aim of his thesis, methodology, sources, structure of his thesis and the reasons that led to the writing of the rigorous thesis. The first chapter is focused on the administrative procedure, its characteristics, division, subject and basic principles of administrative authority operation. In the second chapter, the author defines the subjects of the administrative procedure. The third chapter contains the characteristics of the participants. The author defines the participants according to the Administrative Code. The..
Participants in administrative proceeding and their legal status
No full textParticipants in administrative proceeding and their legal status Abstract This rigorous thesis deals with the participants in administrative proceedings and their legal status. The aim of the thesis is to describe the legal regulation of the participants in administrative proceedings and their selected procedural rights in the Czech Republic, to point out the deficits of the legal regulation and to present proposals de lege ferenda. The author used descriptive method and critical analysis in writing. The thesis also contains a comparison with foreign legislation. The author draws on Czech and foreign sources. The thesis contains the author's observations on the subject. The rigorous thesis is composed of the introduction, eight chapters and the end. In the introduction, the author states the aim of his thesis, methodology, sources, structure of his thesis and the reasons that led to the writing of the rigorous thesis. The first chapter is focused on the administrative procedure, its characteristics, division, subject and basic principles of administrative authority operation. In the second chapter, the author defines the subjects of the administrative procedure. The third chapter contains the characteristics of the participants. The author defines the participants according to the Administrative Code. The...Účastníci správního řízení a jejich právní postavení Abstrakt Tato rigorózní práce se věnuje účastníkům správního řízení a jejich právnímu postavení. Cílem autora je popsat právní úpravu účastníků správního procesu a jejich vybraná procesní práva v České republice, poukázat na deficity právní úpravy a předložit návrhy de lege ferenda. Autor při psaní užil popisnou metodu a kritickou analýzu. Rigorózní práce obsahuje rovněž komparaci se zahraniční právní úpravou. Autor vychází z českých a zahraničních pramenů. Práce obsahuje postřehy autora k předmětné problematice. Rigorózní práce je tvořena úvodem, osmi kapitolami a závěrem. V úvodu autor uvádí cíl práce, metodologii, zdroje, členění práce a důvody, které vedly k sepsání rigorózní práce. První kapitola se věnuje správnímu řízení, jeho charakteristice, členění, předmětu a základním zásadám činnosti správních orgánů. V druhé kapitole vymezuje autor subjekty správního procesu. Třetí kapitola obsahuje charakteristiku účastníků správního řízení. Autor zde vymezuje jednotlivé kategorie účastníků podle správního řádu. Čtvrtá kapitola se zaměřuje na způsobilost být účastníkem. V páté kapitole autor rozebírá procesní způsobilost. V této kapitole se autor rovněž věnuje obecné charakteristice procesní způsobilosti účastníka řízení, postavení nezletilého dítěte, které...Katedra správního práva a správní vědyDepartment of Administrative Law and Administrative ScienceFaculty of LawPrávnická fakult
Equal access to education, its legal framework and protection
No full textEqual access to education, its legal framework and protection Key words: equal access to education, primary school, inclusive education, discrimination Right to equal access to education is the topic of the diploma thesis of the author. Its aim is to analyze some of the problems of the equal access to education with respect to the primary education. The diploma thesis is divided into five chapters. First chapter is introductory and it describes selected problematics of the primary education in the Czech Republic. In the second chapter the concept and conception of equality and discrimination are examined. Third chapter contains regulation of the Czech primary education. The author tries to analyze the Czech primary education, especially pros and contras of its structure. Next she describes the evolution of the primary education with respect to the new regulation, effective from 1.9.2016. In the fourth chapter the author describes some of the aspects of the entrance proceedings; she especially focuses on the entrance criteria. Fifth chapter is devoted to the possible remedies in administrative proceedings, administrative court proceedings and civil proceedings. It refers to some processual aspects, which can be problematic. In the last chapter the author summarizes her conclusions. To sum it up..
Occupancy of buildings and their alterations
No full textThis Diploma Thesis aims on providing a comprehensive overview of an issue re- garding the occupancy of buildings and alterations of the occupancy of buildings. Con- cerned piece of work is divided into eleven chapters in total further structured into sub- chapters. First chapter contains legal sources governing the issue of the occupancy of build- ings and alterations of the occupancy of buildings. The most important legislation con- cerning the issue is listed. This chapter fluently flows into the second chapter which ex- amines next-to-last amendment to the Building Act discussed frequently in the public. At the same time, this chapter establishes seven points to rationalize amendments to the legislation which are accepted positively by the author of this piece of work. Third chapter determines basic definitions contained in the Building Act used in the field of regulation of the occupancy of buildings and alterations of the occupancy of build- ings considered as fundamental for understanding of the concerned matter by the author. This includes general requirements on construction, building, builder and relevant au- thorities. Within nine pages of chapter four the organization of the relevant state author- ities with respect to diverse subject matter jurisdiction of the building authorities is..
The position and activities of an authorized inspector under the Building Act
No full textPresented thesis deals with approved inspector as a major entity of simplified procedure. It is focused on the origin of his function, internal organization and impact of his functioning. Author brings comprehensive view of current problems, primarily from point of view of judicature, specialized literature and comparison with foreign legislation. Author considers a possibility of amendment of the act besides an analysis of current legislation. Grammatical or teleological interpretation of the provisions governing the simplified procedure doesn't bring a satisfactory answer to the question of whether the legislation intended for governing bodies applies on the activity of authorized inspector. Whether through negligence or intent the legislature concerning authorized inspector is conceptual hybrid. In an attempt to clarify its functioning and to define it either a subject of public or private law, concept of simplified procedure deforms to disadvantage of different group of subjects and loses partly its original meaning. The only way out of this confusing situation is amendment of Building Act in favor of unambiquous concept of simplified procedure. The author deals with position of the authorized inspector in initial chapters. The procedure of nomination and assumptions that an applicant must meet..
Legal framework for activities of socalled alternative schools in the Czech Republic
No full text222 Legal Framework for Activities of So-Called Alternative Schools in the Czech Republic Abstract This thesis describes the legal aspects of providing primary and secondary education at so- called alternative schools in the Czech Republic. Firstly, the thesis outlines what an alternative school is and how it can differ from a traditional school. Some examples are given below. Then it explains legal provisions of international human rights law and constitutional law concerning the right to education. A state's obligation to respect parent's philosophical, religious, and other beliefs and rights of individuals and organizations to establish and run private educational institutions lies within the scope of the right to education. The author clarifies why, under these provisions, any quantitative restriction limiting number or capacity of private schools is unacceptable. As a result, some current administrative practices of the Ministry of Education not allowing private schools to be established is labeled as unlawful. Secondly, the author thoroughly describes the general legal framework provided by Education Act nr. 561/2004 Sb., especially its aspects concerning private and alternative schools. This thesis concludes that providing education in schools needs to be seen as an act of the so-called "other"..
WORKS OF JURAJ DALMATINAC IN SPLIT
No full textJuraj Dalmatinac je poznat po svom gotičko renesansnom stilu te po svojoj naturalizaciji
koja je vidljiva na listovima kapitela koji krase kasno gotička djela u Splitu, kao što su to palače
obitelji Papalić i kapele sv. Staša u splitskoj katedrali i blaženog Arnira u Splitu.
U ovom radu, Jurjeva djela su stavljena u opsežniju analizu s palačom kraj Zlatnih vrata
u Dioklecijanovoj palači, koje je izveo sa svojom radionicom i poznatim suradnicima poput
Andrije Alešija i Ivana Pribislavića. Autorica u prvom dijelu rada obrađuje tri palače. Malu i
Veliku palaču Papalić kao i palaču kraj Zlatnih vrata Dioklecijanove palače koje je pripadala
obitelji Ventura Meraviglia. U drugom djelu su obrađene kapele blaženog Arnira i sv. Staša kao
i komad mrežišta pronađen u crvi sv. Roka u Splitu.Georgius Dalmaticus is known by his gothic renaissance style, and by his naturalization
visible on leafs of capital adorning late Gothic works in Split such as palaces Papalić family
and st. Stas's chapel in catedral of Split and blessed Arnir in Split.
In this paper (work) Juraj's work has been put in more extensiv analysis with palace next
to golden gates in Diocletians's palace which he made with his workogruop and many famous
co-workers such as Andrija Aleši and Ivan Pribislavić. In the first part of the paper, the author
deals with three palaces. The Small and Large Papalić palace as well as the palace near the
Golden Gate of Diocletian's Palace which belonged to the Ventura Meraviglia family. In the
second part, the chapels of blessed Arnir and st. Staša as well as a piece of net found in the
worm of St. Roch in Split
The fate of so-called black buildings and some other buildings
No full textThe fate of so-called black buildings and some other buildings Abstract This thesis focuses on the issue of unauthorized buildings in the context of public law. The author examines the concept of building as defined by the new Building Act, the permitting processes through which builders obtain the right to establish a building, and the theoretical definition of unauthorized building in terms of public law, highlighting the classification of unauthorized buildings based on their origin into so-called "black buildings" and "gray buildings." Furthermore, the author describes the classification of unauthorized buildings by their types, the proceedings for ordering the removal of buildings, the conditions for obtaining retrospective permits, and repeated permits. In selected parts of the thesis, the author compares the new legal regulation with the old legal regulation, describing the positive changes brought by the new Building Act compared to the old one, while also focusing on potential weaknesses of the new regulation that may pose practical problems. This work is structured into five parts, with the first part primarily focusing on defining the concept of building in terms of public building law. This section analyzes the definition of building in the new Building Act, describes the characteristics of...Osud tzv. černých a některých dalších staveb Abstrakt Tato diplomová práce se zaměřuje na problematiku neoprávněných staveb ve smyslu veřejného práva. Autor práce se zabývá pojmem stavby ve smyslu nového Stavebního zákona, povolovacích procesů, prostřednictvím kterých stavebníci získají oprávnění zřídit stavbu, teoretického vymezení neoprávněné stavby ve smyslu veřejného práva s poukazem na rozdělení neoprávněných staveb podle jejich vzniku na tzv. černé stavby a tzv. zčernalé stavby. Dále autor popisuje rozdělení neoprávněných staveb podle jejich typů, na řízení o nařízení odstranění stavby, podmínky dodatečného povolení stavby a opakované povolení. Autor ve vybraných částech práce srovnává novou právní úpravu se starou právní úpravou, popisuje pozitivní změny, které nový Stavební zákon přinesl oproti staré právní úpravě a zároveň se zaměřuje na potenciální slabá místa nové právní úpravy, která mohou v praxi činit problémy. Tato práce je strukturována do pěti částí, přičemž první část se zaměřuje zejména na definování pojmu stavby ve smyslu veřejného stavebního práva. Tato část je zaměřena rozbor definice stavby v novém Stavebním zákoně, popsání znaků stavby, které vychází přímo ze zákonné definice stavby a znaků, které dovozuje judikatura a odborná literatura. Druhá část je věnována popisu povolovacích...Katedra správního práva a správní vědyDepartment of Administrative Law and Administrative SciencePrávnická fakultaFaculty of La
